##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780470_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 113684 Sequences flagged as poor quality 0 Sequence length 76 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.995188417015584 34.0 34.0 34.0 32.0 34.0 2 33.3434872101615 34.0 34.0 34.0 33.0 34.0 3 33.50814538545442 34.0 34.0 34.0 34.0 34.0 4 33.534375989585165 34.0 34.0 34.0 34.0 34.0 5 33.529432461911966 34.0 34.0 34.0 34.0 34.0 6 37.28208015200028 38.0 38.0 38.0 36.0 38.0 7 36.88662432708209 38.0 37.0 38.0 35.0 38.0 8 36.92929523943563 38.0 37.0 38.0 35.0 38.0 9 36.69413461876781 38.0 37.0 38.0 34.0 38.0 10-11 36.42114985398121 38.0 37.0 38.0 34.0 38.0 12-13 36.26860420111889 38.0 37.0 38.0 34.0 38.0 14-15 36.48408747053235 38.0 37.0 38.0 34.0 38.0 16-17 36.6061495021287 38.0 37.0 38.0 34.0 38.0 18-19 36.63588983498118 38.0 37.0 38.0 34.0 38.0 20-21 36.38598219626333 38.0 37.0 38.0 34.0 38.0 22-23 36.43565057527884 38.0 37.0 38.0 34.0 38.0 24-25 35.64603198339256 38.0 36.5 38.0 31.5 38.0 26-27 35.84324970972169 38.0 36.5 38.0 32.0 38.0 28-29 35.74656064177897 38.0 36.5 38.0 31.0 38.0 30-31 35.51899563702896 38.0 36.0 38.0 31.0 38.0 32-33 36.00230463389747 38.0 37.0 38.0 33.5 38.0 34-35 35.9326510326871 38.0 37.0 38.0 33.0 38.0 36-37 35.99339396924809 38.0 37.0 38.0 33.5 38.0 38-39 35.48985345343232 38.0 36.5 38.0 29.5 38.0 40-41 35.21939762851413 37.5 36.0 38.0 29.0 38.0 42-43 35.75458287885718 38.0 36.5 38.0 32.5 38.0 44-45 35.96629253017135 38.0 37.0 38.0 33.0 38.0 46-47 36.20846821012631 38.0 37.0 38.0 34.0 38.0 48-49 35.97815875584955 38.0 37.0 38.0 32.5 38.0 50-51 36.114206924457264 38.0 37.0 38.0 34.0 38.0 52-53 35.988265719010585 38.0 37.0 38.0 33.0 38.0 54-55 36.19413461876781 38.0 37.0 38.0 34.0 38.0 56-57 36.19418739664333 38.0 37.0 38.0 34.0 38.0 58-59 36.22743306006122 38.0 37.0 38.0 34.0 38.0 60-61 35.978202737412474 38.0 37.0 38.0 32.5 38.0 62-63 36.32735037472292 38.0 37.0 38.0 34.0 38.0 64-65 36.290551001020376 38.0 37.0 38.0 34.0 38.0 66-67 35.495078463108264 38.0 36.0 38.0 31.0 38.0 68-69 34.352960838816365 37.5 34.5 38.0 24.0 38.0 70-71 33.77971834207101 37.0 34.0 38.0 22.0 38.0 72-73 33.02747088420534 37.0 33.5 38.0 16.0 38.0 74-75 33.451646669716055 37.5 34.0 38.0 21.0 38.0 76 33.048388515534285 37.0 34.0 38.0 19.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.38380797016066026 1101 2 0.4906016266114932 1101 3 0.12573315507844995 1101 4 0.31302720458653255 1101 5 0.21794453494263877 1101 6 -1.0950680212330965 1101 7 0.10448016202064991 1101 8 0.47543343723685894 1101 9 0.2768176420965247 1101 10-11 0.06319966574179148 1101 12-13 0.3490364791851448 1101 14-15 0.3963790703155041 1101 16-17 0.037797426128896916 1101 18-19 -0.311565464244417 1101 20-21 0.003592294818943742 1101 22-23 0.4580078591046828 1101 24-25 -1.8288084867303525 1101 26-27 -0.561037528778904 1101 28-29 0.03983472358392248 1101 30-31 0.7279940047029854 1101 32-33 -0.1648884680345546 1101 34-35 0.15201050181825337 1101 36-37 0.4403179891848694 1101 38-39 0.1873454247598545 1101 40-41 1.36794970166261 1101 42-43 0.5736232113680231 1101 44-45 0.38549588794702316 1101 46-47 0.6807091235730169 1101 48-49 -1.6387875010498547 1101 50-51 -1.8379242757522434 1101 52-53 -2.0002634307482836 1101 54-55 -1.3328605539290308 1101 56-57 -1.2151065978302498 1101 58-59 -1.5806879794408317 1101 60-61 -1.812836735629503 1101 62-63 -1.3041417222064737 1101 64-65 -1.756084128400758 1101 66-67 -1.8826643090282005 1101 68-69 -1.136505044143611 1101 70-71 -1.1956709736811604 1101 72-73 -1.4450421200172343 1101 74-75 -1.1890157750420514 1101 76 -1.882262817130254 1102 1 -0.8391389318333111 1102 2 -0.03210131829901286 1102 3 0.22579289967031713 1102 4 0.28486011887793694 1102 5 0.1757623637245942 1102 6 0.2578109212876285 1102 7 0.038873132073675265 1102 8 0.45927750385208554 1102 9 0.38517939560029646 1102 10-11 0.24112652840391036 1102 12-13 0.3539405144344556 1102 14-15 -0.46033348351686243 1102 16-17 -0.19024643635896865 1102 18-19 -0.49441503234080386 1102 20-21 0.24686480483861573 1102 22-23 0.33898542998915104 1102 24-25 -5.278847071779268 1102 26-27 -1.6683973156898588 1102 28-29 0.007193016880087555 1102 30-31 1.160681764529727 1102 32-33 0.1860425518554223 1102 34-35 0.4894865417475529 1102 36-37 -3.1269382404335104 1102 38-39 -0.41784239730083783 1102 40-41 0.564173345584841 1102 42-43 0.5664227433687259 1102 44-45 0.2571694833267699 1102 46-47 0.9823757487503926 1102 48-49 -3.732356244670875 1102 50-51 -0.8147732464027158 1102 52-53 -0.6058682202730594 1102 54-55 0.15372180648021327 1102 56-57 0.4376715256916981 1102 58-59 -0.6983474850543345 1102 60-61 -5.717606345547104 1102 62-63 -2.641238135041597 1102 64-65 -0.2163601982023522 1102 66-67 1.1517510652467706 1102 68-69 -1.3928280134498223 1102 70-71 -1.9878158045292835 1102 72-73 -5.834365263578512 1102 74-75 -2.2280115680270356 1102 76 -0.9537571787343992 1103 1 0.7079143993030641 1103 2 0.46368601624264727 1103 3 -0.06418348303962773 1103 4 0.3074887781847906 1103 5 0.3273084723641304 1103 6 0.35005016992825944 1103 7 0.6953788716243992 1103 8 0.5112904658996484 1103 9 0.4738485887677655 1103 10-11 0.26870383383588603 1103 12-13 -0.6869119049646741 1103 14-15 -0.1256490214967556 1103 16-17 0.18152815583085413 1103 18-19 0.3971761139216241 1103 20-21 -0.431670923178082 1103 22-23 0.31797702662780836 1103 24-25 -3.6493748907499963 1103 26-27 -0.6171697658463984 1103 28-29 -1.1226581392686512 1103 30-31 0.770051787048537 1103 32-33 0.993698947747113 1103 34-35 0.23530957684394593 1103 36-37 -0.9477544100386055 1103 38-39 -0.40912596955326563 1103 40-41 -0.2722467126345265 1103 42-43 -0.4900115950725379 1103 44-45 1.1001584436167704 1103 46-47 0.9240630118909436 1103 48-49 -2.5829521693152415 1103 50-51 -0.5742723401578615 1103 52-53 -0.13698262681999296 1103 54-55 -0.30442615858816424 1103 56-57 0.32519830110774706 1103 58-59 -0.5062675158117358 1103 60-61 -4.503803961848522 1103 62-63 -1.5509157315704059 1103 64-65 0.06433953267038817 1103 66-67 -0.5187383069727076 1103 68-69 -0.31265555230850595 1103 70-71 -0.004012871591399403 1103 72-73 -4.717201535342117 1103 74-75 -2.2757349711137635 1103 76 -3.007945128424076 1104 1 -0.006290174965407402 1104 2 0.3825155714939257 1104 3 0.22625720867901578 1104 4 -0.16647034753152212 1104 5 -0.1432732001344732 1104 6 -0.058073974298118 1104 7 0.4371507667509249 1104 8 0.3033691717163691 1104 9 0.8135818409610778 1104 10-11 -0.651662310491929 1104 12-13 0.26904935067708635 1104 14-15 0.3017965893354315 1104 16-17 0.3589813735396632 1104 18-19 0.7534872858877506 1104 20-21 -0.019875193171493777 1104 22-23 0.0909117596264366 1104 24-25 0.8250862888230088 1104 26-27 0.9129838930612522 1104 28-29 1.396886462384579 1104 30-31 0.7685506967295552 1104 32-33 0.26516301793322583 1104 34-35 0.2699512929553123 1104 36-37 1.2181870677239601 1104 38-39 1.6792202954703157 1104 40-41 1.7752404824565104 1104 42-43 0.6602242181025773 1104 44-45 0.7742494218611071 1104 46-47 0.8096251600728976 1104 48-49 0.20540423821049103 1104 50-51 0.7022071973440376 1104 52-53 0.64650030842013 1104 54-55 0.15647449560322713 1104 56-57 0.24999671887740504 1104 58-59 -0.6199345374659728 1104 60-61 0.6574668936715327 1104 62-63 0.5560876661678193 1104 64-65 0.8810949462309168 1104 66-67 -0.11513264745650531 1104 68-69 1.6474894854723132 1104 70-71 1.799668856690964 1104 72-73 1.850048325198479 1104 74-75 0.8259588267461737 1104 76 0.33087529857526476 1105 1 0.6445388350100671 1105 2 0.40997988547229625 1105 3 0.3320011681908923 1105 4 -0.19986523375637688 1105 5 -0.2789533595857776 1105 6 -0.027756732649280025 1105 7 0.6310130641393243 1105 8 -0.39245207087041223 1105 9 0.6909157214312245 1105 10-11 0.2425497636451155 1105 12-13 -0.36365575235073067 1105 14-15 0.3629049321948088 1105 16-17 -0.00121540030719558 1105 18-19 0.6199517073394176 1105 20-21 0.3302484011265605 1105 22-23 0.23977693925340304 1105 24-25 0.7159520351273869 1105 26-27 0.44359912886745434 1105 28-29 0.9684587968024161 1105 30-31 1.3000794556083122 1105 32-33 0.8994802205008554 1105 34-35 -0.8887183829638303 1105 36-37 0.28882633846875194 1105 38-39 0.26391902826237157 1105 40-41 0.9443252984329007 1105 42-43 0.5233130008208704 1105 44-45 1.2669998590820413 1105 46-47 1.0812932434331017 1105 48-49 0.1906461996959692 1105 50-51 0.5748757594489504 1105 52-53 0.3037484056541544 1105 54-55 0.7595755888998283 1105 56-57 0.7020440852929752 1105 58-59 0.4477589877560533 1105 60-61 0.8100594428405685 1105 62-63 0.05014732362108987 1105 64-65 0.8292732737509141 1105 66-67 0.5088720032064131 1105 68-69 -0.21067506592434881 1105 70-71 0.8344755513006916 1105 72-73 0.8766968191066908 1105 74-75 -0.39800088053563343 1105 76 0.737300248013959 1106 1 -0.6043540086175199 1106 2 0.10833219132360483 1106 3 0.02699876466435569 1106 4 0.0907876049618821 1106 5 -0.21896747391181748 1106 6 0.03586001225984603 1106 7 -0.8317220467808681 1106 8 0.06769541360894493 1106 9 -0.9385525366768377 1106 10-11 0.3687144512378069 1106 12-13 1.1248840285703778 1106 14-15 -0.5760710632231536 1106 16-17 0.3014039322283679 1106 18-19 0.44467837011953293 1106 20-21 0.1741367956731139 1106 22-23 0.5384739210377489 1106 24-25 -0.5053923032891419 1106 26-27 0.5734816117615082 1106 28-29 0.803561534777117 1106 30-31 0.8210259336748607 1106 32-33 0.24870239840273456 1106 34-35 0.6274261559170498 1106 36-37 0.9335748886819957 1106 38-39 0.09829559439003077 1106 40-41 0.9097196302013799 1106 42-43 1.0407257834909345 1106 44-45 1.1617765356696452 1106 46-47 1.0091721056956828 1106 48-49 1.2399389492280477 1106 50-51 -0.153520628101802 1106 52-53 0.28054670375281177 1106 54-55 -0.00812101445692548 1106 56-57 0.270667324837639 1106 58-59 0.7257651857861873 1106 60-61 0.45881216598499464 1106 62-63 0.40204917791623984 1106 64-65 -0.6508592781805973 1106 66-67 0.5051030690425833 1106 68-69 1.4873550650934177 1106 70-71 0.7197966522181218 1106 72-73 0.302509671325609 1106 74-75 -0.09800497164463451 1106 76 0.49508613982458627 1107 1 0.54769585095395 1107 2 -0.2648901224225142 1107 3 0.04861384162713023 1107 4 0.18238868869300973 1107 5 -0.16464197744981846 1107 6 0.29714639714318025 1107 7 0.5680179371418035 1107 8 0.12218962279286671 1107 9 -0.3798653201390252 1107 10-11 0.5960647683184774 1107 12-13 -0.06487808649168869 1107 14-15 0.25952831353220773 1107 16-17 0.634135192710886 1107 18-19 0.5743886804516976 1107 20-21 0.7617632061610848 1107 22-23 0.16600585240144028 1107 24-25 0.3415157879745152 1107 26-27 -0.288739640086888 1107 28-29 0.9951695655026782 1107 30-31 0.7052691578742127 1107 32-33 0.8804918449257642 1107 34-35 -0.016237688946638684 1107 36-37 1.033442895787779 1107 38-39 0.5829328124164945 1107 40-41 0.7989746146698877 1107 42-43 0.9053536726962932 1107 44-45 0.8408253928890659 1107 46-47 0.8706788095453106 1107 48-49 0.7336320707111312 1107 50-51 0.8399144621595056 1107 52-53 0.6314691653706674 1107 54-55 0.6618024693615396 1107 56-57 0.5475100151108521 1107 58-59 0.656508613433239 1107 60-61 1.2179896418441842 1107 62-63 0.9300554897568958 1107 64-65 0.8355891205371861 1107 66-67 0.6648988274062688 1107 68-69 0.8942306047260189 1107 70-71 0.18122098456233005 1107 72-73 0.8850498894612358 1107 74-75 -0.1856214578548503 1107 76 0.7648776307488347 1108 1 -0.3112384023798569 1108 2 0.245173872429298 1108 3 -0.12056137556209734 1108 4 0.2923329166433888 1108 5 -0.08388869726677228 1108 6 -0.16105137912509804 1108 7 -0.006380547002493131 1108 8 -0.4279426570684137 1108 9 -0.4203089939440403 1108 10-11 0.33612590306518086 1108 12-13 1.0967178521590526 1108 14-15 0.5514020398916557 1108 16-17 -0.17875583728446287 1108 18-19 0.5290560144762182 1108 20-21 0.849706969344382 1108 22-23 0.21061902318546544 1108 24-25 1.0357035181235972 1108 26-27 -0.25338605959281324 1108 28-29 -0.6798822739024217 1108 30-31 -0.007075770801911574 1108 32-33 0.5999334094032562 1108 34-35 0.5638810054153751 1108 36-37 1.2842115490357884 1108 38-39 1.2360690964249343 1108 40-41 0.48727231586040887 1108 42-43 -0.1736031039652488 1108 44-45 0.9257034809088012 1108 46-47 0.8983458575593275 1108 48-49 1.1296314986901095 1108 50-51 0.7108969082643952 1108 52-53 -0.11501379912827758 1108 54-55 -0.36760745520319205 1108 56-57 0.12888447437223505 1108 58-59 0.44828402367956954 1108 60-61 0.9500782335999389 1108 62-63 0.8747392693857989 1108 64-65 0.7285630935806964 1108 66-67 1.0278193522355537 1108 68-69 1.1671159145111503 1108 70-71 -0.8600226248903127 1108 72-73 -0.8758408545236023 1108 74-75 -1.3097902398075831 1108 76 -0.3737033996040324 1109 1 -0.8770944190390892 1109 2 0.19772481694811717 1109 3 0.29357665805384414 1109 4 0.20431417501018956 1109 5 0.019636514692173535 1109 6 -0.2877805179024264 1109 7 -0.6697004934549682 1109 8 0.22072609200193938 1109 9 0.5741131390323133 1109 10-11 0.27822764335969197 1109 12-13 0.32706516734572233 1109 14-15 0.5871375746231706 1109 16-17 0.6261489671183327 1109 18-19 0.5310826394948194 1109 20-21 0.7565565900494207 1109 22-23 0.2518394604902454 1109 24-25 -0.23125319762829832 1109 26-27 0.35359742992080356 1109 28-29 -0.699906816517327 1109 30-31 0.46021933554941796 1109 32-33 0.3583373957188911 1109 34-35 -0.019452327917953482 1109 36-37 0.9666635793793859 1109 38-39 0.6057329369723092 1109 40-41 0.33613071240957026 1109 42-43 1.0011700627757918 1109 44-45 0.748438866897196 1109 46-47 0.8505621300567228 1109 48-49 0.7923564726600674 1109 50-51 0.1242726333871147 1109 52-53 -0.537555819059854 1109 54-55 -0.6236501444788161 1109 56-57 -0.1572972897211784 1109 58-59 -0.20348973803395864 1109 60-61 0.5536034455588847 1109 62-63 0.28792906560772025 1109 64-65 0.6294629894605066 1109 66-67 0.43734634911194803 1109 68-69 -0.10776137241432338 1109 70-71 -0.08730211321397974 1109 72-73 -1.0087800185872666 1109 74-75 -0.8189007559462169 1109 76 0.16110249062354143 1110 1 -0.5046927308759521 1110 2 -0.6238742048639025 1110 3 0.08568212880681614 1110 4 -0.11854702484781399 1110 5 0.2560878222297447 1110 6 -0.236987712317287 1110 7 0.5074383066870283 1110 8 0.491867574272284 1110 9 0.5771601157214619 1110 10-11 -0.520671895156454 1110 12-13 0.288218679750571 1110 14-15 0.6612215094632319 1110 16-17 0.39732003168662544 1110 18-19 -0.270474375454107 1110 20-21 0.6782078739211599 1110 22-23 -0.3515802531531165 1110 24-25 -0.9464792063846588 1110 26-27 1.0839104106331447 1110 28-29 0.24193438570726045 1110 30-31 0.001777533790260577 1110 32-33 0.8514249987638962 1110 34-35 1.0558937849680916 1110 36-37 1.3012541603757128 1110 38-39 0.8373672603265518 1110 40-41 -1.8653345974156963 1110 42-43 -0.11932849755238095 1110 44-45 0.937188718985702 1110 46-47 0.7760788341943012 1110 48-49 1.4354361870922858 1110 50-51 -0.34731356942912583 1110 52-53 0.783880500113419 1110 54-55 -0.13585777276738042 1110 56-57 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2116 38-39 -3.5023812921601234 2116 40-41 -0.6613306283395985 2116 42-43 -1.4925306914482874 2116 44-45 -0.7124857949660353 2116 46-47 -0.7124882639094849 2116 48-49 -0.013630970983804502 2116 50-51 -0.7383711537447297 2116 52-53 0.2863339125375006 2116 54-55 -0.4908392903937866 2116 56-57 -0.20353564981050454 2116 58-59 -2.758334258322165 2116 60-61 -1.9346723729217103 2116 62-63 0.18940064526474742 2116 64-65 -1.6162444642958604 2116 66-67 -0.677051325012684 2116 68-69 -0.8219409957236437 2116 70-71 1.0201379491464522 2116 72-73 0.2761133787919121 2116 74-75 0.4243733771174476 2116 76 -0.9002361517731217 2117 1 0.732654410249971 2117 2 0.6289295849344896 2117 3 0.288744814716253 2117 4 0.16485057347762222 2117 5 0.0698919425338076 2117 6 0.2904442585982139 2117 7 0.6046290084607406 2117 8 0.44752472972011503 2117 9 -0.6081984829344833 2117 10-11 -0.1302014754142533 2117 12-13 -0.9396759991080899 2117 14-15 0.12221633154101141 2117 16-17 -1.065762484563237 2117 18-19 -1.0275912642940312 2117 20-21 -0.02080611748569794 2117 22-23 0.1743809675145087 2117 24-25 -1.5663425973728664 2117 26-27 -1.508631177997458 2117 28-29 -0.510698642826398 2117 30-31 -0.15364826768600182 2117 32-33 -1.157669415690215 2117 34-35 0.6500879019670975 2117 36-37 -0.993457748845394 2117 38-39 -0.040488859536843336 2117 40-41 0.14428311768840274 2117 42-43 0.1687732981951129 2117 44-45 -0.5950796516213259 2117 46-47 -1.0905511096197316 2117 48-49 -0.3952127057137105 2117 50-51 -0.1502165109296172 2117 52-53 0.5272483529282255 2117 54-55 -0.23365440160950612 2117 56-57 0.18045281670709556 2117 58-59 -1.2397924200262977 2117 60-61 -0.1720031955649972 2117 62-63 -0.46763713277456276 2117 64-65 -1.5768482067932155 2117 66-67 -0.8416447109135916 2117 68-69 0.17292189706204653 2117 70-71 -0.428479209850849 2117 72-73 0.5650918417788446 2117 74-75 0.3074615258758371 2117 76 0.24596839100668433 2118 1 -0.5108189025985581 2118 2 0.1949134246977806 2118 3 -0.8481847403082128 2118 4 0.07945168876156572 2118 5 0.31492437681171737 2118 6 0.2165282463073268 2118 7 0.8869551721115911 2118 8 0.7407419824798751 2118 9 -1.3181677557238274 2118 10-11 -0.866436823086957 2118 12-13 -0.786597587818683 2118 14-15 0.7348884608229014 2118 16-17 -3.3374251680729614 2118 18-19 -0.5775685033972522 2118 20-21 -0.17143204214712426 2118 22-23 0.7835592991407765 2118 24-25 0.6807838394087398 2118 26-27 -1.9632800911646413 2118 28-29 -0.18798895806482108 2118 30-31 -0.6418922644994751 2118 32-33 -0.028381831759411114 2118 34-35 0.04077869518435051 2118 36-37 -2.7311270330151842 2118 38-39 -1.679308922533096E-4 2118 40-41 -0.39706205131125216 2118 42-43 0.5022090555639593 2118 44-45 -2.071647308387 2118 46-47 -0.15651218848623927 2118 48-49 0.1828344093426253 2118 50-51 -0.522362863495907 2118 52-53 -1.450906876387812 2118 54-55 -0.5005600576923612 2118 56-57 0.5076122567890309 2118 58-59 -0.4599528843485885 2118 60-61 -0.619755557008034 2118 62-63 -0.9075085337077624 2118 64-65 0.3147245711742386 2118 66-67 0.5851277939230997 2118 68-69 -1.0398754171521318 2118 70-71 -0.09703503095019528 2118 72-73 0.9636408346091656 2118 74-75 0.4865784030807987 2118 76 0.5277141517896595 2119 1 0.462944660808148 2119 2 -0.7827796683525321 2119 3 -0.01815173700788364 2119 4 -0.06826775944225005 2119 5 -0.2029187744098806 2119 6 -0.6022622658374104 2119 7 -0.9350881833030869 2119 8 0.11212408471887869 2119 9 -0.14404566725598755 2119 10-11 -0.3468722767042536 2119 12-13 -0.01427287333284255 2119 14-15 -0.08383675702262394 2119 16-17 -0.27335365300779557 2119 18-19 -0.4788316512947759 2119 20-21 -1.24820626633214 2119 22-23 -0.009618040175894293 2119 24-25 0.8456519449243274 2119 26-27 0.3400416079117434 2119 28-29 -0.12563290231535262 2119 30-31 0.9977781773648502 2119 32-33 0.3710827549870288 2119 34-35 0.2797083435448755 2119 36-37 -0.19817121944821992 2119 38-39 -1.5410718414271898 2119 40-41 -1.317302096898544 2119 42-43 -0.14759981021470736 2119 44-45 0.04197070809090775 2119 46-47 -0.1250462721104313 2119 48-49 -0.4733198751505725 2119 50-51 0.4282627746074681 2119 52-53 0.0464991591797741 2119 54-55 -0.2033641510513391 2119 56-57 -1.6719411302043454 2119 58-59 -0.10766639670701039 2119 60-61 0.44615607012589464 2119 62-63 -0.5073791322784089 2119 64-65 0.1719734255560752 2119 66-67 0.7144579931300683 2119 68-69 0.6872359630506324 2119 70-71 0.1172786666066088 2119 72-73 0.42905859940722735 2119 74-75 -0.3005265664089549 2119 76 0.17888826200643848 >>END_MODULE >>Per sequence quality scores pass #Quality Count 16 1.0 17 1.0 18 1.0 19 0.0 20 2.0 21 5.0 22 14.0 23 24.0 24 65.0 25 134.0 26 270.0 27 524.0 28 859.0 29 1429.0 30 2229.0 31 3404.0 32 5136.0 33 7877.0 34 11344.0 35 15682.0 36 23359.0 37 41324.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.43270820871891 21.07156679919778 10.616269659758629 30.87945533232469 2 19.50406389641462 19.141655817881144 38.892896097955735 22.461384187748497 3 17.353365469195314 25.072129763203264 34.258118996516664 23.31638577108476 4 12.465254565286232 21.34249322683931 35.4385841455262 30.753668062348265 5 11.908447978607366 37.58312515393547 35.88983498117589 14.618591886281271 6 24.100137222476338 37.887477569402904 17.820449667499386 20.191935540621373 7 24.203933710988355 34.111220576334404 19.905175750325462 21.779669962351782 8 21.22990042574153 35.463213820766335 22.156152141022485 21.15073361246965 9 20.885964603638154 19.543647303050562 18.95957214735583 40.61081594595545 10-11 20.85033953766581 24.859258998627777 29.613226135603952 24.67717532810246 12-13 23.415784103303892 20.67441328595053 27.49331480243482 28.41648780831076 14-15 22.896361845114527 23.681872559023258 21.68994757397699 31.73181802188523 16-17 23.888146089159424 27.656046585271454 22.30393019246332 26.1518771331058 18-19 22.60036592660357 27.002920375778473 22.903838710812426 27.492874986805532 20-21 21.22638190070722 25.11435206361493 23.080204778156997 30.579061257520845 22-23 22.387934977657366 29.549892684986457 23.429858203441118 24.632314133915063 24-25 23.6167798458886 30.5733436543401 23.23326061714929 22.576615882622004 26-27 22.093698321663556 28.81408113718729 28.168431793392212 20.92378874775694 28-29 19.33649414165582 24.312568171422537 29.276767179198483 27.07417050772316 30-31 18.596284437563774 25.624978009218534 30.41369058090848 25.36504697230921 32-33 18.239154146581754 30.13792618134478 27.732134689138316 23.890784982935152 34-35 18.949016572252912 30.6665845677492 31.01535836177474 19.369040498223143 36-37 18.949016572252912 28.060237148587312 30.748830090426093 22.241916188733683 38-39 20.65726047640829 24.836828401534078 28.68037718588368 25.825533936173954 40-41 19.862953449913796 24.982407374828473 30.313852433060063 24.840786742197672 42-43 24.029326906160936 23.693747581014037 31.358854368248828 20.918071144576196 44-45 19.83788395904437 25.363287709792054 29.865240491186096 24.93358783997748 46-47 23.75532176911439 22.03432321170965 28.441557299180182 25.768797719995778 48-49 21.450248056014917 21.896660919742445 29.467207346680276 27.185883677562366 50-51 17.913690580908483 22.233999507406494 32.81420428556349 27.038105626121528 52-53 18.729548573238098 22.29645332676542 35.86476549030647 23.109232609690018 54-55 17.547324161711412 22.574416804475565 33.17661236409697 26.701646669716055 56-57 21.57955385102565 22.523398191478133 30.278227367087716 25.618820590408504 58-59 17.885542380634035 27.30595334435805 32.13776784771824 22.670736427289683 60-61 19.317582069596426 32.67522254670842 26.33308117237254 21.674114211322614 62-63 16.28681256817142 37.234791175539215 24.125206713345765 22.353189542943596 64-65 16.220400408148905 38.470233278209776 22.868213644840083 22.44115266880124 66-67 16.221719855036767 37.928820238556 22.153073431617464 23.69638647478977 68-69 16.048432497097217 36.1581225150417 22.614000211111502 25.179444776749587 70-71 16.536188029977833 34.43052672319764 23.253052320467262 25.78023292635727 72-73 16.604359452517507 32.45487491643503 23.655923436895254 27.28484219415221 74-75 16.768410682242006 32.11401780373667 23.804581119594665 27.312990394426656 76 16.23095598325182 32.45839344146933 23.74212730023574 27.5685232750431 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 0.5 11 0.0 12 0.0 13 1.0 14 2.0 15 3.0 16 8.5 17 13.0 18 18.0 19 24.5 20 32.0 21 38.0 22 47.5 23 75.0 24 135.5 25 178.0 26 245.5 27 340.5 28 439.0 29 510.0 30 652.0 31 891.0 32 1209.0 33 1430.0 34 1653.0 35 2163.5 36 2890.5 37 3330.0 38 4785.5 39 7740.5 40 9423.5 41 9403.0 42 9199.0 43 8521.5 44 7791.5 45 7605.0 46 7471.0 47 7484.5 48 7097.0 49 6336.0 50 5976.0 51 5591.0 52 4759.5 53 3919.5 54 3526.0 55 3307.0 56 2707.5 57 2045.0 58 1763.0 59 1453.5 60 985.5 61 697.0 62 567.0 63 474.5 64 317.0 65 209.0 66 114.0 67 62.0 68 48.5 69 23.0 70 7.0 71 3.0 72 3.0 73 1.5 74 0.0 75 0.0 76 0.0 77 0.5 78 0.5 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 113684.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.077759403258156 #Duplication Level Percentage of deduplicated Percentage of total 1 59.615469031210225 23.892544245452306 2 17.55629691409508 14.072340874705326 3 8.875817567270971 10.671686429048943 4 4.718844651244458 7.564828823757081 5 2.543786488740617 5.097463143450265 6 1.626355296080067 3.9108405756306954 7 1.110574601641719 3.115653917877626 8 0.8033009964444054 2.5755603251117134 9 0.5004170141784821 1.8050033425987826 >10 2.587682718054519 15.494704619823372 >50 0.030727360519731354 0.9033813025579677 >100 0.019753303191255872 1.4769008831497836 >500 0.004389622931390193 1.0370852538615811 >1k 0.00658443439708529 8.382006262974562 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGT 4331 3.8096829808944093 No Hit ATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTCTT 3922 3.4499137961366597 Illumina PCR Primer Index 2 (95% over 21bp) CTTTACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTC 1276 1.1224094859434925 No Hit CTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTC 657 0.5779177368846979 No Hit TCTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCG 522 0.45916751697688324 No Hit TATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTCT 338 0.2973153653988248 No Hit TCTTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCC 265 0.23310228352274726 No Hit CTCTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCC 258 0.22694486471271244 No Hit CTTAATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCG 189 0.1662503078709405 No Hit CTTATACACATCTCCGAGCCCACGAGACTTCCATATATATCGTATGCCGT 145 0.1275465324935787 No Hit TCTTAATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCC 142 0.12490763871784948 No Hit CTTATAACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCG 130 0.11435206361493262 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.004398156292882024 0.0 2 0.0 0.0 0.0 0.004398156292882024 0.0 3 0.0 0.0 0.0 0.005277787551458428 0.0 4 0.0 0.0 0.0 0.006157418810034833 0.0 5 0.0 0.0 0.0 0.008796312585764047 0.0 6 0.0 0.0 0.0 0.008796312585764047 0.0 7 0.0 0.0 0.0 0.008796312585764047 0.0 8 0.0 0.0 0.0 0.008796312585764047 0.0 9 0.0 0.0 0.0 0.011435206361493262 0.0 10 0.0 0.0 0.0 0.012314837620069666 0.0 11 0.0 0.0 0.0 0.012314837620069666 0.0 12 0.0 0.0 0.0 0.013194468878646072 0.0 13 0.0 0.0 0.0 0.013194468878646072 0.0 14 0.0 0.0 0.0 0.014074100137222476 0.0 15 0.0 0.0 0.0 0.016712993912951692 0.0 16 0.0 0.0 0.0 0.02023151894725731 0.0 17 0.0 0.0 0.0 0.02199078146441012 0.0 18 0.0 0.0 0.0 0.024629675240139332 0.0 19 0.0 0.0 0.0 0.029027831533021357 0.0 20 0.0 0.0 0.0 0.029907462791597762 0.0 21 0.0 0.0 0.0 0.03166672530875057 0.0 22 0.0 0.0 0.0 0.03166672530875057 0.0 23 0.0 0.0 0.0 0.0378241441187854 0.0 24 0.0 0.0 0.0 0.04574082544597305 0.0 25 0.0 0.0 0.0 0.04750008796312586 0.0 26 0.0 0.0 0.0 0.051018612997431476 0.0 27 0.0 0.0 0.0 0.08004644453045283 0.0 28 0.0 0.0 0.0 0.10467611977059217 0.0 29 0.0 0.0 0.0 0.1310650575278843 0.0 30 0.0 0.0 0.0 0.17416698919812815 0.0 31 0.0 0.0 0.0 0.2243059709369832 0.0 32 0.0 0.0 0.0 0.2920375778473664 0.0 33 0.0 0.0 0.0 0.3624080785334788 0.0 34 0.0 0.0 0.0 0.44421378558108443 0.0 35 0.0 0.0 0.0 0.595510362056226 0.0 36 0.0 0.0 0.0 0.7485662010485205 0.0 37 0.0 0.0 0.0 1.0617149291017205 0.0 38 0.0 0.0 0.0 1.4302804264452342 0.0 39 0.0 0.0 0.0 1.888568312163541 0.0 40 0.0 0.0 0.0 2.5122268744942122 0.0 41 0.0 0.0 0.0 3.292459800851483 0.0 42 0.0 0.0 0.0 4.25213750395834 0.0 43 0.0 0.0 0.0 5.167833644136378 0.0 44 0.0 0.0 0.0 6.0333908025755605 0.0 45 0.0 0.0 0.0 6.898947961014743 0.0 46 0.0 0.0 0.0 7.692375356250659 0.0 47 0.0 0.0 0.0 8.446219344850638 0.0 48 0.0 0.0 0.0 9.107702051300095 0.0 49 0.0 0.0 0.0 9.776221807818162 0.0 50 0.0 0.0 0.0 10.504556489919425 0.0 51 0.0 0.0 0.0 11.2548819534851 0.0 52 0.0 0.0 0.0 12.084374230322648 0.0 53 0.0 0.0 0.0 13.012385208120756 0.0 54 0.0 0.0 0.0 13.979099961296225 0.0 55 0.0 0.0 0.0 14.767249568980683 0.0 56 0.0 0.0 0.0 15.522852820097816 0.0 57 0.0 0.0 0.0 16.21424298933887 0.0 58 0.0 0.0 0.0 16.830864501600928 0.0 59 0.0 0.0 0.0 17.467717532810244 0.0 60 0.0 0.0 0.0 18.055311213539284 0.0 61 0.0 0.0 0.0 18.63850673797544 0.0 62 0.0 0.0 0.0 19.285915344287673 0.0 63 0.0 0.0 0.0 19.986101826114492 0.0 64 0.0 0.0 0.0 20.67397347032124 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTGCGA 20 7.897963E-5 70.00001 10 CATCGTT 15 0.0022197352 70.0 32 GCTAATC 15 0.0022197352 70.0 14 TTTACAC 205 0.0 61.463417 2 ATCTCTT 20 0.006936009 52.500004 37 TCGTTGT 20 0.006936009 52.500004 34 CCGTGCG 20 0.006936009 52.500004 9 TGACGAT 20 0.006936009 52.500004 42 GTAATCG 20 0.006936009 52.500004 21 ACGATTC 20 0.006936009 52.500004 24 GCATTTC 20 0.006936009 52.500004 54 CTTATAA 40 7.424278E-7 52.500004 1 GGTGCTA 20 0.006936009 52.500004 37 GAACGAA 20 0.006936009 52.500004 67 GGTGCAC 20 0.006936009 52.500004 1 GATCTCT 20 0.006936009 52.500004 36 CCAACGA 20 0.006936009 52.500004 16 GCATGTA 20 0.006936009 52.500004 2 CTATACA 130 0.0 51.15385 1 GCGAGAA 35 2.0755095E-5 50.0 13 >>END_MODULE