FastQCFastQC Report
Wed 25 May 2016
SRR1780469_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780469_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences521323
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGGTA551.3763201E-731.98639126
TCGGTCA651.7502316E-830.44858229
CAGGTAA454.0238687E-529.37724320
GAACGCG300.00570558229.37159317
GGTACAT300.00574860629.3264871
CAGTTGT554.9629416E-627.9934641
CGTGTAC407.012661E-427.50942436
TCGTTCG655.907568E-727.08360123
CGGTGGT1500.026.37864743
TGTTCGT450.001398266424.44108410
TTGTCCG450.001402181524.42935831
TTAGCGC551.6006836E-423.98058742
CTTAGCG551.6006836E-423.98058741
TCGGTGG1850.023.76454542
TTCGGTC752.0681528E-623.45668628
CGGTAAG752.0681528E-623.45668628
TCGGTAA752.0681528E-623.45668627
CGTTCGG904.0479063E-722.01388224
CGAGCTT602.863095E-422.00753837
ATTGTCC500.002581982221.99486530
Produced by FastQC (version 0.11.5)