FastQCFastQC Report
Wed 25 May 2016
SRR1780469_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780469_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences521323
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGT251384.821962583657348No Hit
ATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGTCTT208834.005769935337593RNA PCR Primer, Index 24 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGTC60101.1528361495656245No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCG35210.6753970187388625No Hit
CTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGTC31610.6063419415602228No Hit
TATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGTCT17880.342973549987244No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCC16720.32072246956301564No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCC13500.258956539419899No Hit
CTTAATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCG8770.16822584079351957No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCC7510.14405656378099566No Hit
CTTATAACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCG6280.12046274574496042No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT21750.042.2760444
GCCGTCT24400.037.6845843
TTCCATG250.002352777235.19631617
TTTACAC7650.033.0779532
CTATACA3950.032.90151
TCGTTGT756.184564E-1132.2632934
CTTATAA651.7220373E-830.4993061
CTTTACA8400.030.4186211
CATCGTT801.3096724E-1030.24683632
TTACACA8550.029.853423
GGAATAT300.005707385529.3697011
GGTCTAA300.005707385529.3697011
GTATCAA300.005744992829.33026134
TGCCGTC34550.026.61371442
TTATACA40550.025.558322
CTTATAC41550.025.2346151
CTTTATA2200.025.0045782
AACGCTG450.001398000424.44188537
GTCGTGG551.5899468E-424.00439514
TCTTTAT2300.023.942691
Produced by FastQC (version 0.11.5)