FastQCFastQC Report
Wed 25 May 2016
SRR1780468_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780468_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13172
Sequences flagged as poor quality0
Sequence length76
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCCGC150.00217618270.01
GTCCGCC150.00217618270.02
CTTACGC150.00217618270.012
ATTATAC200.00680094952.51
GACGAAC200.00680094952.523
TGTACGC200.00680094952.51
CTTATAC1051.0913936E-1136.6666681
TTATACA1350.036.2962952
CCGACGA903.8326107E-935.00000421
TGCCGAC1054.5110937E-1033.33333619
CTGCCGA1054.5110937E-1033.33333618
GCTGCCG1001.07229425E-831.49999817
TATACAC1450.031.379313
GCCGACG901.5457044E-731.11111320
CGACGAA604.7245473E-429.16666622
CATCTCA500.006958172727.9999988
CGCTGCC1108.9524656E-725.45454816
CATCTGA700.001163256425.0000029
ATACACA1901.6370905E-1123.947374
ACGCTGC1051.8493722E-523.33333412
Produced by FastQC (version 0.11.5)