##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780468_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 13172 Sequences flagged as poor quality 0 Sequence length 76 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 19.065593683571212 12.0 12.0 23.0 12.0 32.0 2 18.228135438809595 12.0 11.0 23.0 11.0 32.0 3 19.01989067719405 21.0 11.0 23.0 11.0 32.0 4 20.3020801700577 21.0 11.0 31.0 11.0 33.0 5 20.29175523838445 21.0 11.0 31.0 11.0 33.0 6 19.617977528089888 11.0 11.0 27.0 11.0 34.0 7 21.743546917704222 21.0 11.0 31.0 11.0 36.0 8 20.00576981475858 21.0 11.0 28.0 11.0 34.0 9 21.07660188278166 21.0 12.0 31.0 10.0 35.0 10-11 20.245141208624354 21.0 11.0 28.0 10.5 34.0 12-13 20.45422107500759 21.0 11.0 29.5 11.0 34.0 14-15 20.09554357728515 21.0 11.0 27.5 11.0 34.0 16-17 19.67210750075919 16.5 11.0 27.0 11.0 34.0 18-19 20.09516398420893 21.0 11.0 27.5 11.0 34.0 20-21 20.186418159732767 16.0 11.0 29.0 11.0 34.0 22-23 19.47361828120255 16.0 11.0 27.0 10.5 34.0 24-25 19.74229426055269 16.0 11.0 27.0 10.5 34.0 26-27 19.33886273914364 11.5 11.0 26.0 10.5 34.0 28-29 18.770687822654114 11.0 11.0 26.0 11.0 34.0 30-31 18.79084421500152 11.0 11.0 26.0 11.0 34.0 32-33 19.321932887944122 16.0 11.0 26.0 11.0 34.0 34-35 19.4260552687519 16.0 11.0 26.5 11.0 34.0 36-37 19.095809292438503 11.0 11.0 26.0 11.0 34.0 38-39 18.561190403887032 11.0 11.0 26.0 11.0 34.0 40-41 18.866383237169757 11.0 11.0 26.0 11.0 34.0 42-43 19.080132098390525 11.0 11.0 26.0 11.0 34.0 44-45 19.01924536896447 11.0 11.0 26.0 11.0 34.0 46-47 19.17389158821743 11.0 11.0 26.0 11.0 34.0 48-49 19.3958396598846 16.0 11.0 26.0 11.0 34.0 50-51 19.512526571515338 21.0 11.0 26.0 11.0 34.0 52-53 19.360006073489217 15.5 11.0 26.0 11.0 34.0 54-55 19.479881566960216 20.5 11.0 26.0 11.0 34.0 56-57 18.786251138779228 15.0 11.0 26.0 11.0 33.5 58-59 18.67916793197692 15.0 11.0 26.0 11.0 33.5 60-61 19.062291223808078 19.0 11.0 26.0 11.0 33.0 62-63 18.606210142726997 15.0 11.0 25.5 11.0 33.0 64-65 18.884717582751293 15.0 11.0 25.5 11.0 33.0 66-67 19.391056787124203 19.0 11.0 26.0 11.0 33.0 68-69 18.762868205283937 11.0 11.0 25.0 11.0 33.0 70-71 18.862701184330398 13.5 11.0 25.0 11.0 33.0 72-73 18.58005617977528 11.0 11.0 25.0 11.0 32.5 74-75 18.836774977224415 15.0 11.0 25.0 11.0 33.0 76 18.5705283935621 19.0 11.0 25.0 11.0 32.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -1.2731780039407568 1101 2 0.26179682760062306 1101 3 0.6788056801426947 1101 4 -1.6531548259069098 1101 5 0.8670965420919146 1101 6 -0.9671577603208732 1101 7 -0.4062752799853371 1101 8 0.3746613422659557 1101 9 1.5763306973540736 1101 10-11 -1.469743501019721 1101 12-13 2.2840835441624243 1101 14-15 0.18408044303962257 1101 16-17 0.9250543750709959 1101 18-19 -2.3113086571219625 1101 20-21 -0.25364046515311856 1101 22-23 0.8500667951851923 1101 24-25 0.0469211145252828 1101 26-27 0.6427345032419183 1101 28-29 0.6631596131672133 1101 30-31 1.0473782534088123 1101 32-33 1.8240711954794513 1101 34-35 0.5169810245925888 1101 36-37 0.5100811031755477 1101 38-39 1.4329552056758388 1101 40-41 1.4296139340951868 1101 42-43 0.11002771274198508 1101 44-45 0.6310450885918115 1101 46-47 0.5024198024148347 1101 48-49 1.6659888992255851 1101 50-51 -0.60720088967064 1101 52-53 -2.04240669804042 1101 54-55 -1.8276400797790657 1101 56-57 0.6482402742124584 1101 58-59 0.5151516726871321 1101 60-61 -1.2053240478285403 1101 62-63 -0.15600655086792514 1101 64-65 0.023006501371551025 1101 66-67 -0.45614461132241146 1101 68-69 -1.8218917984078793 1101 70-71 0.6931872908106342 1101 72-73 -1.4230511863669904 1101 74-75 -0.8905115004360056 1101 76 -1.2188914853065285 1102 1 -1.72772345848621 1102 2 -1.4654758996721036 1102 3 -2.1393761380391254 1102 4 -2.683457856209941 1102 5 -1.648054973059601 1102 6 -0.5732183663814787 1102 7 -2.8911237648338215 1102 8 -2.8677629001582865 1102 9 -0.6963965753731998 1102 10-11 -3.3485313798076035 1102 12-13 -1.0340982740193922 1102 14-15 -1.4674347084755297 1102 16-17 -1.7113092612926408 1102 18-19 -0.6749450207583223 1102 20-21 -1.3900041015167588 1102 22-23 -1.0438725987542021 1102 24-25 -1.6197455521413815 1102 26-27 0.3245526850601017 1102 28-29 -0.2004767504691536 1102 30-31 -1.8920156859851254 1102 32-33 -1.2062318348235763 1102 34-35 -0.19514018752862228 1102 36-37 -2.095979502885058 1102 38-39 -2.1276508549302235 1102 40-41 0.23264423712549132 1102 42-43 -0.20815410543983504 1102 44-45 -1.1871367295900086 1102 46-47 0.42666222665725684 1102 48-49 -0.7127989795622938 1102 50-51 -0.8496251320948822 1102 52-53 -0.7090733647070877 1102 54-55 -2.1155188676578547 1102 56-57 -4.033577907605721 1102 58-59 -2.439393781858321 1102 60-61 -2.432596775101267 1102 62-63 -1.5650974599588316 1102 64-65 -0.8709328925678435 1102 66-67 -3.7288718840496813 1102 68-69 -3.9128008893169692 1102 70-71 -3.5189339213105804 1102 72-73 -1.2866875500033537 1102 74-75 -0.057178167102669875 1102 76 -3.5219217883368312 1103 1 2.5132225873378857 1103 2 1.4295721417174008 1103 3 2.571636426631976 1103 4 2.4429279531027 1103 5 0.6076730387659737 1103 6 0.9278902810686311 1103 7 1.9152324805468126 1103 8 0.515829117580072 1103 9 -0.23150374462671053 1103 10-11 1.7608551686033351 1103 12-13 1.52909463063693 1103 14-15 1.980828410519294 1103 16-17 3.5750913299860017 1103 18-19 1.8849219415476597 1103 20-21 0.6018658171824285 1103 22-23 1.5230157974024898 1103 24-25 1.1973276185903252 1103 26-27 -0.3871989779110727 1103 28-29 0.6088358881117806 1103 30-31 1.6408741883681657 1103 32-33 1.242770382471324 1103 34-35 1.2039728945112884 1103 36-37 0.7251587084970552 1103 38-39 -0.63060724813052 1103 40-41 1.2714468978793683 1103 42-43 1.5830506247892835 1103 44-45 -0.46910273106820455 1103 46-47 -0.692332599654641 1103 48-49 -0.7501234437160242 1103 50-51 1.3942773069295065 1103 52-53 -0.25933204911211405 1103 54-55 0.00569325355426642 1103 56-57 0.46198750259383203 1103 58-59 1.3577237347713904 1103 60-61 1.3929760260812891 1103 62-63 -0.2524588790275679 1103 64-65 0.6626960800855173 1103 66-67 -0.3559967916623954 1103 68-69 -0.008883668326575389 1103 70-71 -2.473848925006074 1103 72-73 0.48160513292347673 1103 74-75 -0.40159797493711125 1103 76 0.9104507172064054 1104 1 -0.06338779415054674 1104 2 -1.278995713191918 1104 3 -0.8480008466638331 1104 4 -0.0773972501493354 1104 5 -0.5618078868125131 1104 6 -0.0370878302509432 1104 7 -0.8002146739247316 1104 8 0.47955644716105894 1104 9 0.4457945668179413 1104 10-11 0.019766988490765414 1104 12-13 -1.3079910479121644 1104 14-15 0.2610035199626992 1104 16-17 0.7933527433693648 1104 18-19 -1.5572294030427045 1104 20-21 -0.8259015374141931 1104 22-23 -3.2058772607588644 1104 24-25 -1.324873757269586 1104 26-27 -2.7430463825389673 1104 28-29 -0.838005887998289 1104 30-31 0.29096799699856035 1104 32-33 -0.1887493173410597 1104 34-35 1.170827178438742 1104 36-37 -0.5703384772440323 1104 38-39 0.3315566042772353 1104 40-41 -1.2463767418954887 1104 42-43 -0.3118837091694395 1104 44-45 0.5296464871932081 1104 46-47 0.09565989565492572 1104 48-49 2.0237977570344405 1104 50-51 -0.32398410645385667 1104 52-53 -0.94100809664182 1104 54-55 1.0697958176568285 1104 56-57 1.0142076401798263 1104 58-59 -0.42307676554130325 1104 60-61 -0.45474129724578916 1104 62-63 -1.6583375531989226 1104 64-65 0.19083866920371761 1104 66-67 -0.41884857402637365 1104 68-69 -0.05266102917710924 1104 70-71 1.715331812955153 1104 72-73 1.341517578201774 1104 74-75 1.402029292104789 1104 76 -1.123320389735433 1105 1 1.3725096417468912 1105 2 -1.278995713191918 1105 3 -0.48902648768947543 1105 4 -0.7184228911749742 1105 5 -1.741295066299692 1105 6 -3.1909339840970965 1105 7 -0.5950864687965236 1105 8 -2.4948025271979155 1105 9 -1.4260003049769274 1105 10-11 -1.0186945499707747 1105 12-13 1.2561115161903977 1105 14-15 -1.0851503261911475 1105 16-17 -2.0271600771434564 1105 18-19 -0.17261401842732482 1105 20-21 0.6228164113037593 1105 22-23 -1.50074905563066 1105 24-25 -1.4530788854747172 1105 26-27 0.26977413028154373 1105 28-29 -0.2354417854341868 1105 30-31 -1.4910832850527242 1105 32-33 -1.2656723942641364 1105 34-35 0.6323656399772055 1105 36-37 0.3270974201918655 1105 38-39 1.9982232709439032 1105 40-41 0.01003351451476675 1105 42-43 0.9573470600613305 1105 44-45 -1.4959945384478175 1105 46-47 -0.263314463319432 1105 48-49 -0.46338173014504136 1105 50-51 0.5734517909820411 1105 52-53 0.7641201084863845 1105 54-55 -0.22507597721496353 1105 56-57 0.9757461017182862 1105 58-59 -0.2948716373361755 1105 60-61 0.9683356258311342 1105 62-63 -1.0942349890963605 1105 64-65 -1.2194177410526912 1105 66-67 -0.9829511381289358 1105 68-69 -1.3731738496899304 1105 70-71 -1.3359502383268982 1105 72-73 -3.158482421798226 1105 74-75 0.5943369844124824 1105 76 1.825397558982516 1106 1 1.9924191440093324 1106 2 -1.429825275786186 1106 3 -0.3042602735114954 1106 4 -0.02837764230619655 1106 5 -0.3815665595123612 1106 6 -0.49033066584672014 1106 7 0.1311578750948783 1106 8 -0.15920675042566756 1106 9 0.9729438880849095 1106 10-11 1.2267805631514008 1106 12-13 1.8228535976383604 1106 14-15 1.3409431881376612 1106 16-17 -0.7104179956954937 1106 18-19 0.8451084551775025 1106 20-21 2.208035114169519 1106 22-23 1.207018666842945 1106 24-25 -0.6638631992002075 1106 26-27 -1.1687628229612876 1106 28-29 -0.2953965365654092 1106 30-31 0.8463978612519512 1106 32-33 -0.872007235893097 1106 34-35 -0.605567995015253 1106 36-37 0.2667655951541583 1106 38-39 0.21013880638884785 1106 40-41 -0.3515803618049915 1106 42-43 -2.1569063336038283 1106 44-45 0.9670522187165851 1106 46-47 -0.9993627287794631 1106 48-49 -0.31217509364428864 1106 50-51 -1.5260957203301757 1106 52-53 -0.3784138281652005 1106 54-55 -0.8178361582104401 1106 56-57 -0.3063051803329948 1106 58-59 -0.29901945030751875 1106 60-61 0.4234638309593386 1106 62-63 -0.36723649739198905 1106 64-65 -1.297849113601714 1106 66-67 -0.11568115321189154 1106 68-69 -0.6258133670353274 1106 70-71 1.3518325671029672 1106 72-73 0.9191948029378239 1106 74-75 0.23649384715757904 1106 76 -1.1957185797806815 1107 1 0.3761726454098948 1107 2 -0.80097373516994 1107 3 0.3021823035193165 1107 4 1.970221797469712 1107 5 -1.3731631981678234 1107 6 -1.0645603577234724 1107 7 2.9378805641705092 1107 8 1.5088604764650881 1107 9 1.7516553726787478 1107 10-11 0.9501699188936961 1107 12-13 0.7213129813918648 1107 14-15 0.7436042525634292 1107 16-17 -0.44291099289436886 1107 18-19 -0.25503160084490517 1107 20-21 -0.8185755300881858 1107 22-23 2.176906622025019 1107 24-25 0.582635400239571 1107 26-27 -0.9719841114766972 1107 28-29 2.1079648079724045 1107 30-31 -0.940716984686425 1107 32-33 -2.140214518806264 1107 34-35 -0.7925427849312179 1107 36-37 0.8802109733054202 1107 38-39 -1.9047071319865019 1107 40-41 0.6666269211081719 1107 42-43 -1.2633489106346403 1107 44-45 0.948144655691376 1107 46-47 -0.6177100677150378 1107 48-49 0.47984171307839674 1107 50-51 -0.8377203701901195 1107 52-53 -0.26426816990189295 1107 54-55 0.7556932535542664 1107 56-57 0.4794091053812899 1107 58-59 0.22619063372609816 1107 60-61 1.744892402387908 1107 62-63 -0.3140152088765795 1107 64-65 0.34010606847111546 1107 66-67 0.500565345387546 1107 68-69 0.5947748682587886 1107 70-71 -1.8533495057261646 1107 72-73 -1.9176399309557333 1107 74-75 0.9633846034600992 1107 76 -1.2881555545705972 1108 1 -1.2661307875912122 1108 2 -1.5789360828997303 1108 3 0.06851674427236176 1108 4 0.7520601141917496 1108 5 0.017202250337160052 1108 6 -0.42945515285082436 1108 7 -1.110292193304577 1108 8 0.6184950279601047 1108 9 -0.7992859340765115 1108 10-11 -0.008855551759680225 1108 12-13 -0.7581997539348251 1108 14-15 -0.017469944557277017 1108 16-17 -0.5182155333151925 1108 18-19 1.3208266494319503 1108 20-21 0.07123620856076229 1108 22-23 -0.6879881731023367 1108 24-25 -0.0150943893506863 1108 26-27 2.58670913326306 1108 28-29 0.6652737780720734 1108 30-31 2.2838123619359436 1108 32-33 -0.9588745409546569 1108 34-35 -0.789220525795006 1108 36-37 -1.0417159369935867 1108 38-39 0.4153371648019828 1108 40-41 -0.2240153823248292 1108 42-43 3.092409671868129 1108 44-45 0.1948223965551641 1108 46-47 0.9534416487855175 1108 48-49 0.0014363974637809918 1108 50-51 -1.884508853025114 1108 52-53 -0.21083516879447473 1108 54-55 0.6684839512286835 1108 56-57 -0.7714214594027631 1108 58-59 0.34496139784569735 1108 60-61 2.312998714680269 1108 62-63 1.5458961975464511 1108 64-65 -1.4093402216728492 1108 66-67 0.8289152900165639 1108 68-69 -1.945922806159814 1108 70-71 -1.3314779664127627 1108 72-73 -0.007617782561492703 1108 74-75 -0.037445961323953725 1108 76 -1.779849906730064 1109 1 -0.21509719585994702 1109 2 1.1077564235602182 1109 3 -1.495436744099731 1109 4 0.7837138609617753 1109 5 -0.976337801342428 1109 6 -2.1439254370885514 1109 7 0.5053408816308256 1109 8 0.3898128574174713 1109 9 -0.9665985955752205 1109 10-11 0.8509635696873481 1109 12-13 2.8661542512331337 1109 14-15 2.2674137763729547 1109 16-17 1.930104880121501 1109 18-19 2.8402064943931897 1109 20-21 4.123884787372134 1109 22-23 1.930874875993272 1109 24-25 -0.31418999658582436 1109 26-27 0.21849207899949263 1109 28-29 0.07225052225811979 1109 30-31 0.11682269785325872 1109 32-33 -1.2592621378538809 1109 34-35 1.572536580148146 1109 36-37 0.9179093860038314 1109 38-39 0.23219762991826087 1109 40-41 0.6606745401557923 1109 42-43 -0.52128541857115 1109 44-45 3.2913986239453443 1109 46-47 1.8584804084754367 1109 48-49 0.019524252760941607 1109 50-51 -2.3635140209837715 1109 52-53 -0.5083157889495098 1109 54-55 0.13069325355426642 1109 56-57 1.4159170418892266 1109 58-59 2.541666824202288 1109 60-61 1.7984638309593386 1109 62-63 0.3288419339805593 1109 64-65 0.6278044811695302 1109 66-67 0.11961296443516645 1109 68-69 -0.6135584650745471 1109 70-71 -0.3156510930277534 1109 72-73 0.0327568944410892 1109 74-75 -1.5028852378097426 1109 76 1.9856539692389283 1110 1 -1.9455776763404273 1110 2 -1.8749763091725136 1110 3 0.7951037964408094 1110 4 -1.3296495024015869 1110 5 1.0071456821410543 1110 6 1.6233418301787168 1110 7 0.9205060467959889 1110 8 2.385308352912965 1110 9 0.14226026328363872 1110 10-11 1.1580206267444026 1110 12-13 1.0346977947766796 1110 14-15 1.409305668264846 1110 16-17 -0.72154677153015 1110 18-19 -0.11474846056176347 1110 20-21 -0.07018428169693536 1110 22-23 1.9053493504677483 1110 24-25 2.1234976911018606 1110 26-27 -0.3806070200996068 1110 28-29 1.0452234952310953 1110 30-31 1.0518827579133188 1110 32-33 1.157404528812787 1110 34-35 0.09355760116916656 1110 36-37 0.5616781547725971 1110 38-39 -0.18334291062227948 1110 40-41 -0.48234347786223 1110 42-43 3.115351218065488 1110 44-45 2.0282604858072055 1110 46-47 1.0769488769439093 1110 48-49 1.340845574082259 1110 50-51 1.069293786824037 1110 52-53 0.26308060744688433 1110 54-55 0.0327202805812945 1110 56-57 -0.2117105857383983 1110 58-59 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60-61 -0.3765361690406621 2116 62-63 -0.8211580660194393 2116 64-65 2.5805822589473095 2116 66-67 1.269612964435165 2116 68-69 0.34477486825878856 2116 70-71 -0.46287331524997555 2116 72-73 1.479979116663312 2116 74-75 2.2943369844124817 2116 76 3.7023206359055933 2117 1 2.292839312076559 2117 2 2.103788169591965 2117 3 2.433134684471696 2117 4 1.2321265593744748 2117 5 1.0554082304036037 2117 6 1.673534880371765 2117 7 -0.097833721543779 2117 8 1.4731461907508034 2117 9 0.5254648964882733 2117 10-11 3.1406461093698894 2117 12-13 -1.844163209084325 2117 14-15 1.1245566335158124 2117 16-17 1.2118509118675327 2117 18-19 2.024730303917 2117 20-21 0.8183292318165769 2117 22-23 0.7542875744059714 2117 24-25 1.5231115907157644 2117 26-27 -0.037460301952886965 2117 28-29 1.8043933794009739 2117 30-31 1.3926163486469072 2117 32-33 -0.07473832833007066 2117 34-35 -0.7032570706455061 2117 36-37 1.4694966875911284 2117 38-39 -1.1249452272245932 2117 40-41 1.8511507306319785 2117 42-43 1.3378415655558378 2117 44-45 1.376716084262803 2117 46-47 2.29895659895163 2117 48-49 2.1762702845069697 2117 50-51 -0.6353394178091669 2117 52-53 2.551208020574297 2117 54-55 2.2378361106971205 2117 56-57 0.7472662482384358 2117 58-59 1.5476192051546676 2117 60-61 0.494892402387908 2117 62-63 1.108603838742468 2117 64-65 1.0603441637092104 2117 66-67 -0.09467274985054885 2117 68-69 2.1304891539730733 2117 70-71 3.0514123990357405 2117 72-73 1.374026735710931 2117 74-75 -0.2151868251113278 2117 76 1.5332730168579758 2118 1 -0.9325301460515192 2118 2 2.056990140035314 2118 3 -2.0902643303066277 2118 4 -1.0314202386550804 2118 5 -2.0702075331431935 2118 6 -1.2722779275100073 2118 7 1.6250726823971071 2118 8 2.2231461907508034 2118 9 -0.04226909365950959 2118 10-11 2.143109163557078 2118 12-13 0.17123087958562522 2118 14-15 1.3277586039591611 2118 16-17 0.060373079355215964 2118 18-19 -2.251747528595317 2118 20-21 1.5640188869889897 2118 22-23 -1.9273626718994485 2118 24-25 0.10439237889310249 2118 26-27 -0.46541596697751686 2118 28-29 1.5894919015684614 2118 30-31 0.007148368351344914 2118 32-33 -0.4977678849803162 2118 34-35 0.3158315993052341 2118 36-37 0.5821814166551711 2118 38-39 2.133059698883777 2118 40-41 1.9034906321098148 2118 42-43 -0.7760746906017992 2118 44-45 1.9345978576618208 2118 46-47 -0.16163453405329875 2118 48-49 1.0463441761325853 2118 50-51 0.9262369368706338 2118 52-53 0.2574888087516385 2118 54-55 2.9884518742439248 2118 56-57 0.4178327507014892 2118 58-59 0.8678162494896426 2118 60-61 1.7941534861317514 2118 62-63 2.5759683707621726 2118 64-65 -1.8688430284090138 2118 66-67 -1.0355594493579368 2118 68-69 -0.08625961449983066 2118 70-71 1.7940232364741604 2118 72-73 1.2133124499966463 2118 74-75 -1.1240538201852175 2118 76 0.3620907508481217 2119 1 -0.13176386252661487 2119 2 -0.10520653940274372 2119 3 -1.782473781136769 2119 4 -2.8922120649641485 2119 5 -1.5504118754165006 2119 6 -1.1624439556070705 2119 7 0.45904458533452797 2119 8 -0.9250019573973454 2119 9 0.6167347377581152 2119 10-11 -2.917554948831171 2119 12-13 -2.0273642672853853 2119 14-15 -0.8344380754788965 2119 16-17 -2.014339564322942 2119 18-19 -1.5301638759771805 2119 20-21 -1.0751892867019421 2119 22-23 -0.9765325314141329 2119 24-25 -0.027152959548789823 2119 26-27 0.45923281974023666 2119 28-29 -1.7610828110752124 2119 30-31 1.1029338089643659 2119 32-33 -0.6203732489649916 2119 34-35 -0.4830189754074077 2119 36-37 -1.0589424658480233 2119 38-39 -0.06872829600766295 2119 40-41 -0.2606217561405053 2119 42-43 -0.03980393708966545 2119 44-45 -0.4447124871657664 2119 46-47 -0.6322603322653038 2119 48-49 -0.4619572287205429 2119 50-51 -1.5533288357985846 2119 52-53 -3.059241714875437 2119 54-55 0.33902658688759857 2119 56-57 -0.32482369885151385 2119 58-59 -1.1666665091310477 2119 60-61 -3.7709806134851043 2119 62-63 0.680693785832414 2119 64-65 1.8361378145028624 2119 66-67 -0.6396462948240931 2119 68-69 -0.19226216877824953 2119 70-71 -0.8202807226573796 2119 72-73 -1.5829838462996513 2119 74-75 0.18692957700507407 2119 76 0.08750582109077953 >>END_MODULE >>Per sequence quality scores fail #Quality Count 12 40.0 13 250.0 14 689.0 15 1039.0 16 1353.0 17 1549.0 18 1704.0 19 1685.0 20 1449.0 21 1072.0 22 756.0 23 463.0 24 330.0 25 244.0 26 165.0 27 116.0 28 65.0 29 45.0 30 40.0 31 32.0 32 21.0 33 18.0 34 9.0 35 11.0 36 17.0 37 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 6.006878104700038 27.09209017959496 31.218952999617883 35.682078716087126 2 18.000303674460977 22.85150318858184 33.09292438505922 26.055268751897962 3 4.760097175827513 26.6246583662314 43.63802004251443 24.97722441542666 4 3.423929547525053 25.30367446097783 23.147585788035226 48.12481020346189 5 2.938050409960522 29.054054054054053 19.936228363194655 48.07166717279077 6 19.268144549043427 29.221075007591864 12.503795930762223 39.006984512602486 7 5.1548739750986945 32.99423018524142 10.302156088672943 51.54873975098694 8 14.864864864864865 33.98876404494382 13.741269359246889 37.40510173094443 9 14.599149711509263 27.262374734284844 10.750075918615245 47.388399635590645 10-11 8.22957789249924 28.91740054661403 24.100364409353173 38.752657151533555 12-13 7.3868812632857574 25.82751290616459 24.312936532037654 42.472669298511995 14-15 12.978779941540447 29.924458110313935 18.942413544395095 38.15434840375052 16-17 16.918463407227453 29.20209535378075 18.353325235347707 35.52611600364409 18-19 12.30261160036441 27.43319161858488 17.336015791071972 42.92818098997874 20-21 9.99848162769511 28.382174309140602 18.535529911934407 43.08381415122988 22-23 19.1808381415123 28.609930154873975 14.356210142726997 37.85302156088673 24-25 13.695718190100212 35.484360765259645 14.151229881566959 36.668691163073184 26-27 10.184482235044033 36.027178864257515 20.391740054661405 33.396598846037044 28-29 14.219556635286972 30.22699665958093 24.058609170968722 31.494837534163377 30-31 13.657758882477983 29.057849984816276 25.728818706346797 31.555572426358943 32-33 9.319010021257213 36.74840570907987 21.87974491345278 32.05283935621014 34-35 9.804889158821743 34.72517461281506 20.539781354388094 34.9301548739751 36-37 10.863953841481932 30.30671120558761 23.538566656544184 35.29076829638627 38-39 11.186607956270878 24.8177953234133 28.17339811721834 35.822198603097476 40-41 8.791375645308229 27.376252657151532 25.254327361068935 38.5780443364713 42-43 4.930914060127543 25.762982083206804 29.02368660795627 40.282417248709386 44-45 6.3164287883389 28.19237777102946 30.83054965077437 34.66064378985727 46-47 6.984512602490131 32.45520801700577 24.78742787731552 35.772851503188576 48-49 7.303370786516854 35.397054357728514 19.268144549043427 38.03143030671121 50-51 7.105982386881263 32.481779532341335 18.899939265107804 41.5122988156696 52-53 6.4986334649256 30.811569996963257 21.507743698754936 41.18205283935621 54-55 6.620103249316732 31.16079562708776 19.294716064378985 42.92438505921652 56-57 10.636197995748557 31.077285150318858 17.996507743698757 40.29000911023383 58-59 9.326601882781658 29.930914060127545 18.13316125113878 42.60932280595202 60-61 6.388551472821136 28.22654114788946 19.082143941694504 46.3027634375949 62-63 9.193744306103858 29.99544488308533 17.18797449134528 43.622836319465534 64-65 9.45945945945946 31.270877619192227 14.230944427573641 45.038718493774674 66-67 7.094594594594595 39.23094442757364 12.169754023686608 41.504706954145156 68-69 8.051169146674765 38.255390221682354 15.5671120558761 38.12632857576678 70-71 6.863042818098998 41.37564530822958 15.908745824476162 35.85256604919526 72-73 7.595657455208017 39.15502581232918 16.371849377467353 36.877467354995446 74-75 7.094594594594595 40.4342544791983 13.411023382933497 39.06012754327361 76 5.488915882174309 35.203461888855145 14.295475250531432 45.01214697843912 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 1.0 9 0.5 10 0.5 11 3.0 12 5.0 13 3.5 14 2.5 15 3.0 16 4.0 17 5.0 18 8.0 19 10.0 20 11.0 21 13.0 22 17.5 23 20.5 24 22.0 25 25.0 26 36.5 27 51.0 28 60.5 29 67.0 30 81.0 31 110.0 32 130.5 33 136.0 34 162.5 35 214.0 36 270.0 37 301.0 38 336.0 39 401.0 40 484.0 41 562.0 42 587.0 43 643.0 44 719.5 45 774.0 46 808.0 47 848.5 48 847.5 49 833.0 50 860.0 51 807.5 52 720.5 53 659.0 54 632.0 55 608.5 56 542.0 57 459.5 58 420.0 59 376.5 60 302.5 61 243.5 62 215.0 63 181.0 64 138.5 65 107.0 66 73.5 67 63.0 68 63.5 69 48.5 70 28.5 71 24.0 72 23.0 73 17.0 74 13.0 75 14.0 76 15.0 77 10.0 78 5.0 79 6.0 80 6.0 81 7.0 82 7.0 83 6.0 84 5.5 85 4.5 86 4.0 87 4.0 88 2.5 89 2.0 90 2.0 91 2.0 92 3.0 93 1.5 94 1.0 95 2.5 96 3.0 97 3.0 98 2.5 99 3.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.6604919526267841 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0037959307622228975 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 13172.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.82538718493774 #Duplication Level Percentage of deduplicated Percentage of total 1 99.8859228838695 99.71150926207106 2 0.07605141075366949 0.1518372304889159 3 0.03042056430146779 0.09110233829334953 4 0.0 0.0 5 0.0 0.0 6 0.007605141075366947 0.045551169146674765 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.007591861524445794 0.0 2 0.0 0.0 0.0 0.007591861524445794 0.0 3 0.0 0.0 0.0 0.007591861524445794 0.0 4 0.0 0.0 0.0 0.007591861524445794 0.0 5 0.0 0.0 0.0 0.007591861524445794 0.0 6 0.0 0.0 0.0 0.007591861524445794 0.0 7 0.0 0.0 0.0 0.007591861524445794 0.0 8 0.0 0.0 0.0 0.007591861524445794 0.0 9 0.0 0.0 0.0 0.007591861524445794 0.0 10 0.0 0.0 0.0 0.007591861524445794 0.0 11 0.0 0.0 0.0 0.015183723048891588 0.0 12 0.0 0.0 0.0 0.015183723048891588 0.0 13 0.0 0.0 0.0 0.015183723048891588 0.0 14 0.0 0.0 0.0 0.015183723048891588 0.0 15 0.0 0.0 0.0 0.015183723048891588 0.0 16 0.0 0.0 0.0 0.015183723048891588 0.0 17 0.0 0.0 0.0 0.015183723048891588 0.0 18 0.0 0.0 0.0 0.015183723048891588 0.0 19 0.0 0.0 0.0 0.022775584573337383 0.0 20 0.0 0.0 0.0 0.022775584573337383 0.0 21 0.0 0.0 0.0 0.022775584573337383 0.0 22 0.0 0.0 0.0 0.030367446097783177 0.0 23 0.0 0.0 0.0 0.045551169146674765 0.0 24 0.0 0.0 0.0 0.053143030671120556 0.0 25 0.0 0.0 0.0 0.06073489219556635 0.0 26 0.0 0.0 0.0 0.06073489219556635 0.0 27 0.0 0.0 0.0 0.06073489219556635 0.0 28 0.0 0.0 0.0 0.06832675372001215 0.0 29 0.0 0.0 0.0 0.08351047676890373 0.0 30 0.0 0.0 0.0 0.09869419981779533 0.0 31 0.0 0.0 0.0 0.10628606134224111 0.0 32 0.0 0.0 0.0 0.10628606134224111 0.0 33 0.0 0.0 0.0 0.11387792286668691 0.0 34 0.0 0.007591861524445794 0.0 0.1214697843911327 0.0 35 0.0 0.007591861524445794 0.0 0.1290616459155785 0.0 36 0.0 0.007591861524445794 0.0 0.15942909201336167 0.0 37 0.0 0.007591861524445794 0.0 0.23534770725781962 0.0 38 0.0 0.007591861524445794 0.0 0.30367446097783174 0.0 39 0.0 0.007591861524445794 0.0 0.3795930762222897 0.0 40 0.0 0.007591861524445794 0.0 0.4099605223200729 0.0 41 0.0 0.007591861524445794 0.0 0.44791982994230184 0.0 42 0.0 0.007591861524445794 0.0 0.4858791375645308 0.0 43 0.0 0.007591861524445794 0.0 0.5238384451867598 0.0 44 0.0 0.007591861524445794 0.0 0.5845733373823262 0.0 45 0.0 0.007591861524445794 0.0 0.6073489219556635 0.0 46 0.0 0.007591861524445794 0.0 0.6453082295778925 0.0 47 0.0 0.007591861524445794 0.0 0.6604919526267841 0.0 48 0.0 0.007591861524445794 0.0 0.6680838141512299 0.0 49 0.0 0.007591861524445794 0.0 0.6756756756756757 0.0 50 0.0 0.007591861524445794 0.0 0.698451260249013 0.0 51 0.0 0.007591861524445794 0.0 0.7136349832979046 0.0 52 0.0 0.007591861524445794 0.0 0.7288187063467962 0.0 53 0.0 0.007591861524445794 0.0 0.7591861524445794 0.0 54 0.0 0.007591861524445794 0.0 0.7971454600668084 0.0 55 0.0 0.007591861524445794 0.0 0.8275129061645916 0.0 56 0.0 0.007591861524445794 0.0 0.8275129061645916 0.0 57 0.0 0.007591861524445794 0.0 0.8502884907379289 0.0 58 0.0 0.007591861524445794 0.0 0.8882477983601579 0.0 59 0.0 0.007591861524445794 0.0 0.9034315214090495 0.0 60 0.0 0.007591861524445794 0.0 0.9034315214090495 0.0 61 0.0 0.007591861524445794 0.0 0.9337989675068327 0.0 62 0.0 0.007591861524445794 0.0 0.9489826905557243 0.0 63 0.0 0.007591861524445794 0.0 0.9641664136046159 0.0 64 0.0 0.007591861524445794 0.0 0.9869419981779533 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGTCCGC 15 0.002176182 70.0 1 GTCCGCC 15 0.002176182 70.0 2 CTTACGC 15 0.002176182 70.0 12 ATTATAC 20 0.006800949 52.5 1 GACGAAC 20 0.006800949 52.5 23 TGTACGC 20 0.006800949 52.5 1 CTTATAC 105 1.0913936E-11 36.666668 1 TTATACA 135 0.0 36.296295 2 CCGACGA 90 3.8326107E-9 35.000004 21 TGCCGAC 105 4.5110937E-10 33.333336 19 CTGCCGA 105 4.5110937E-10 33.333336 18 GCTGCCG 100 1.07229425E-8 31.499998 17 TATACAC 145 0.0 31.37931 3 GCCGACG 90 1.5457044E-7 31.111113 20 CGACGAA 60 4.7245473E-4 29.166666 22 CATCTCA 50 0.0069581727 27.999998 8 CGCTGCC 110 8.9524656E-7 25.454548 16 CATCTGA 70 0.0011632564 25.000002 9 ATACACA 190 1.6370905E-11 23.94737 4 ACGCTGC 105 1.8493722E-5 23.333334 12 >>END_MODULE