FastQCFastQC Report
Wed 25 May 2016
SRR1780468_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780468_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13172
Sequences flagged as poor quality0
Sequence length76
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGT348126.427269966595805No Hit
ATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTCTT259819.723656240510174RNA PCR Primer, Index 15 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTC8266.270877619192226No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG4193.1809899787427875No Hit
CTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTC3292.497722441542666No Hit
TATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTCT2321.7613118736714242No Hit
CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATATCGTATGCCGT2041.5487397509869418No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCC1230.9337989675068328No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCC970.736410567871242No Hit
CTTATACACATCTCCGAGCCCACGAGACCGCGGACAAACTCGTATGCCGT960.7288187063467962No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCC940.7136349832979046No Hit
CTTAATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG890.6756756756756757No Hit
ATACACATCTCCGAGCCCACGAGACCGCGGACAATATCGTATGCCGTCTT830.6301245065290009No Hit
CTTATACACATCTCCGAGCCCACGAGACCGCGGACAACCTCGTATGCCGT820.6225326450045551No Hit
CTTATAACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG820.6225326450045551No Hit
ATACACATCTCCGAGCCCACGAGACCGCGGACAAACTCGTATGCCGTCTT730.5542058912845429No Hit
TTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTC680.516246583662314No Hit
CTTAACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTC610.46310355299119343No Hit
CTTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG480.3644093531733981No Hit
ATACACATCTCCGAGCCCACGAGACCGCGGACAACCTCGTATGCCGTCTT470.3568174916489523No Hit
CTTATCACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTC440.33404190707561493No Hit
CTTATTACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG380.2884907379289402No Hit
CTTATACACATCTCCGAGCCCACGCGACCGCGGACAATCTCGTATGCCGT370.2808988764044944No Hit
CTTATACACATCTCCGAGCCCACGAGACAGCGGACAATCTCGTATGCCGT370.2808988764044944No Hit
CTTATACACATCTCCGAGCCCACGAGACCGCGGACCATCTCGTATGCCGT360.2733070148800486No Hit
ATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTCTGCCGTCTT290.22016398420892802No Hit
ATACACATCTCCGAGCCCACGAGACCGCGGACACTCTCGTATGCCGTCTT280.21257212268448225TruSeq Adapter, Index 19 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCGCGGCCAATCTCGTATGCCGT270.20498026116003645No Hit
ACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTCTTCT270.20498026116003645RNA PCR Primer, Index 15 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTAGTATGCCGT260.19738839963559063No Hit
ATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTCTTCTGCTT250.18979653811114486RNA PCR Primer, Index 36 (96% over 27bp)
TCTTATACACATCTCCGAGCCCACGAGACCGCGGACAACCTCGTATGCCG250.18979653811114486No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGCGGACAATATCGTATGCCG250.18979653811114486No Hit
CTTACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTCT240.18220467658669906No Hit
TCTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGT240.18220467658669906No Hit
CTTATACACATCTCCGAGCACACGAGACCGCGGACAATCTCGTATGCCGT240.18220467658669906No Hit
CTTTACACATCTCCGAGCCCACGAGACCGCGGACAATATCGTATGCCGTC240.18220467658669906No Hit
TCTTTACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGT210.1594290920133617No Hit
CTTATACACATCTCCGAGCCCACGAGACCGCGGACACTCTCGTATGCCGT210.1594290920133617No Hit
CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTCTGCCGT210.1594290920133617No Hit
ATACACATCTCCGAGCCCACGAGACCGCGGACCATCTCGTATGCCGTCTT200.1518372304889159Illumina PCR Primer Index 7 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACCGCGGACAAACTCGTATGCCGTC190.1442453689644701No Hit
CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCACGTATGCCGT190.1442453689644701No Hit
CTTTACACATCTCCGAGCCCACGAGACCGCGGACAACCTCGTATGCCGTC180.1366535074400243No Hit
CTTATACACATCTCCGAGCCCACGTGACCGCGGACAATCTCGTATGCCGT180.1366535074400243No Hit
CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCAGT180.1366535074400243No Hit
CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCCCGTATGCCGT170.1290616459155785No Hit
ATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCAGTCTT160.12146978439113272No Hit
ATACACATCTCCGAGCCCACGAGACCGCGGCCAATCTCGTATGCCGTCTT160.12146978439113272Illumina PCR Primer Index 4 (95% over 22bp)
TCTTATACACATCTCCGAGCCCACGAGACCGCGGACAAACTCGTATGCCG160.12146978439113272No Hit
CTTATACACATATCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGT160.12146978439113272No Hit
ATACACCTCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTCTT160.12146978439113272RNA PCR Primer, Index 15 (95% over 21bp)
CTTATACACATCTACGAGCCCACGAGACCGCGGACAATCTCGTATGCCGT150.11387792286668692No Hit
CTTATACACATCTCCGCGCCCACGAGACCGCGGACAATCTCGTATGCCGT150.11387792286668692No Hit
CTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTCTTCTGCTTGA140.10628606134224113RNA PCR Primer, Index 15 (96% over 28bp)
ATACACATCTACGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTCTT140.10628606134224113RNA PCR Primer, Index 36 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCGCGGACAAAATCGTATGCCGT140.10628606134224113No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTACA1400.070.000011
CTTTATA150.00217618270.01
TTGAACA150.00217618270.055
CTTGAAC150.00217618270.054
TTTATAC150.00217618270.02
TTTACAC1450.067.5862052
TTACACA1600.061.253
CTTATAC5750.058.4347841
TTATACA5950.055.882352
TAAAAAA200.00680094952.561
GACAATA552.0045263E-950.90909633
TATACAC6700.050.1492543
CAATATC505.5973942E-848.99999635
TCTTATA1000.048.9999961
ACAATAT505.5973942E-848.99999634
CTATACA651.5825208E-1048.4615361
AACCAAC305.668809E-446.66666470
TATCGTA551.1851989E-744.54545638
AATATCG551.1851989E-744.54545636
ATATCGT551.1851989E-744.54545637
Produced by FastQC (version 0.11.5)