FastQCFastQC Report
Wed 25 May 2016
SRR1780467_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780467_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101822
Sequences flagged as poor quality0
Sequence length50
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTGACGCTGCCGACGAGACCCAATGTGTAGATCTCGGTG1670.16401170670385573No Hit
ATACACATCTGACGCTGCCGACGAGACCCAATGTGTAGATCTCGGTGGTC1260.1237453595490169No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTGTA404.0775194E-738.49741734
ATTTCGG404.09329E-738.478542
CTTTACA1500.036.7002941
TTTACAC1500.036.646192
CGAACTT250.002337826235.21495425
GAACTTC250.002337826235.21495426
CTATACA654.5292836E-1033.8771971
GTATATC353.1985994E-431.42646438
GACGAAT353.1985994E-431.42646423
TTACACA1900.031.2303543
CTTATAC7850.030.2952751
TTATACA7750.030.073522
GTAAGCA300.00570874929.34579529
GTAGTTC300.00572249829.33136738
ATCTCCG300.005736273229.31695242
ACATCTA300.00575007529.3025558
CCTCTGA300.00575007529.3025559
GGTGGTC1001.8189894E-1228.58402844
CTTAATA852.5465852E-1028.49671
TTAATAC852.582965E-1028.4546892
Produced by FastQC (version 0.11.5)