##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780467_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 101822 Sequences flagged as poor quality 0 Sequence length 50 %GC 54 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 18.06129323721789 16.0 10.0 27.0 7.0 30.0 2 18.313861444481546 16.0 10.0 27.0 7.0 31.0 3 18.592249219225707 16.0 10.0 28.0 7.0 31.0 4 20.43211683133311 19.0 10.0 32.0 7.0 35.0 5 20.561096816012256 19.0 10.0 32.0 7.0 35.0 6 20.774007581858537 17.0 10.0 32.0 8.0 35.0 7 20.50071693740056 17.0 10.0 31.0 8.0 35.0 8 20.518110035159395 17.0 10.0 31.0 8.0 35.0 9 20.501325843138027 17.0 10.0 31.0 8.0 35.0 10 20.391683526153482 17.0 10.0 31.0 8.0 35.0 11 20.62495334996366 17.0 10.0 31.0 8.0 35.0 12 20.41832806269765 17.0 10.0 31.0 8.0 37.0 13 20.355846477185676 17.0 10.0 31.0 8.0 35.0 14 20.56789298972717 17.0 10.0 31.0 8.0 37.0 15 20.396093182219953 17.0 10.0 31.0 8.0 37.0 16 20.289495393922728 17.0 9.0 31.0 7.0 36.0 17 20.4037830724205 17.0 9.0 31.0 7.0 37.0 18 20.08400934964939 17.0 8.0 30.0 7.0 36.0 19 20.159552945335978 18.0 8.0 30.0 7.0 37.0 20 20.25007365795211 18.0 8.0 30.0 7.0 37.0 21 20.063051207008307 18.0 8.0 30.0 7.0 37.0 22 20.096364243483727 18.0 8.0 30.0 7.0 37.0 23 19.966844100489087 17.0 8.0 30.0 7.0 37.0 24 19.545893814696235 17.0 8.0 30.0 7.0 36.0 25 19.45167056235391 17.0 8.0 30.0 7.0 36.0 26 19.132692345465617 16.0 8.0 30.0 7.0 36.0 27 18.927088448468897 16.0 8.0 29.0 7.0 36.0 28 18.790889984482725 16.0 8.0 28.0 7.0 35.0 29 18.49482430123156 16.0 8.0 28.0 7.0 35.0 30 18.31582565653788 15.0 8.0 28.0 7.0 35.0 31 18.204621790968552 15.0 8.0 27.0 7.0 35.0 32 17.914360354343856 15.0 8.0 27.0 7.0 35.0 33 17.910942625365834 15.0 8.0 27.0 7.0 35.0 34 17.530003339160494 15.0 8.0 26.0 7.0 34.0 35 17.434955117754512 15.0 8.0 26.0 7.0 34.0 36 17.35084755750231 14.0 8.0 26.0 7.0 34.0 37 17.165416118324135 14.0 8.0 26.0 7.0 33.0 38 17.146048987448683 13.0 8.0 26.0 7.0 33.0 39 16.85221268488146 13.0 8.0 25.0 7.0 33.0 40 16.307379544695646 13.0 8.0 24.0 7.0 33.0 41 16.43282394767339 13.0 8.0 24.0 7.0 33.0 42 16.501934748875488 13.0 8.0 24.0 7.0 33.0 43 16.491249435289035 13.0 8.0 24.0 7.0 33.0 44 16.50854432244505 13.0 8.0 24.0 7.0 33.0 45 16.561676258568877 13.0 8.0 24.0 7.0 33.0 46 16.354373318143427 13.0 8.0 24.0 7.0 32.0 47 16.277798511127262 13.0 8.0 24.0 7.0 32.0 48 16.20107638820687 13.0 8.0 24.0 7.0 32.0 49 15.886409616782228 12.0 8.0 23.0 7.0 32.0 50 15.891202294199681 12.0 8.0 23.0 7.0 32.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.17053407130275744 1101 2 -0.12771463441646347 1101 3 -0.34799091242075164 1101 4 0.15507948362942514 1101 5 0.753151809536039 1101 6 -0.12796050013391636 1101 7 -0.03836123557552895 1101 8 0.05211179526342846 1101 9 -0.33223748367094075 1101 10 -0.5184913395536164 1101 11 -0.7844935361637582 1101 12 -0.7194299211826483 1101 13 -0.7288113997466468 1101 14 -0.44184893098780265 1101 15 0.01820129620505284 1101 16 -0.04641072201925667 1101 17 -0.3129674902648958 1101 18 -0.1835292037970504 1101 19 -0.42355250163894453 1101 20 -1.0445673174952574 1101 21 -1.0250554985119145 1101 22 -1.070041777519851 1101 23 -1.4297310559744005 1101 24 -1.5621012448061649 1101 25 -1.0936632551498917 1101 26 -1.3590503934594906 1101 27 -1.6068149609275402 1101 28 -1.7108052577088877 1101 29 -0.9678908480034742 1101 30 -0.989496907384023 1101 31 -1.1903611122793798 1101 32 -0.5998524668003888 1101 33 -0.9028561804243331 1101 34 -1.0686443570895072 1101 35 -1.8816130773038395 1101 36 -2.2845789986470564 1101 37 -1.4103026077827003 1101 38 -1.6542529216985287 1101 39 -1.8385112511396589 1101 40 -2.0969695697629103 1101 41 -1.724682415970543 1101 42 -1.209903369494933 1101 43 -1.7450698026816927 1101 44 -0.84302192933683 1101 45 -0.7006665279113875 1101 46 -1.053239023916717 1101 47 -0.4477152683568928 1101 48 -0.7729990813652883 1101 49 -0.05558219812397169 1101 50 -0.43920016438114473 1102 1 -0.840797229197495 1102 2 -0.44034621336383495 1102 3 0.16139505249153174 1102 4 -0.7426398146161901 1102 5 -0.40869029572711923 1102 6 -1.1180482194321613 1102 7 -0.49809807768079395 1102 8 -1.2004320643856943 1102 9 -0.3752199398112914 1102 10 -0.5247194097290553 1102 11 -0.6641426589707748 1102 12 -1.7088158860949285 1102 13 -0.6324956102729615 1102 14 -0.831234895900085 1102 15 -1.0661846687072298 1102 16 -0.7408844062297852 1102 17 -0.09165170079121054 1102 18 -0.7560730634461734 1102 19 -0.980219168305613 1102 20 -0.18018135258297718 1102 21 -0.947423919564546 1102 22 -0.6233751108531855 1102 23 0.34649701420103796 1102 24 -1.0960486132272145 1102 25 -0.8953299218165576 1102 26 -0.810892498722648 1102 27 -1.2500605749626281 1102 28 -0.9887877138492378 1102 29 -1.5482417251964549 1102 30 -0.05283024071735554 1102 31 -0.4805365508758719 1102 32 -0.19660685276529932 1102 33 0.15609118799671862 1102 34 0.030127572735054287 1102 35 0.4598781507663361 1102 36 0.10261398380908204 1102 37 0.013469322041860465 1102 38 -0.5884634480143198 1102 39 -0.42473932131509784 1102 40 0.702504114447617 1102 41 0.5018965313978789 1102 42 -0.141920913354582 1102 43 -0.5637540132080083 1102 44 0.17925877241755472 1102 45 0.7009124194570333 1102 46 -0.20394077830268031 1102 47 0.46842508252030157 1102 48 1.1091061817926047 1102 49 0.9581020124023425 1102 50 0.7787822917592067 1103 1 -0.014643660343853782 1103 2 -0.26442696318358827 1103 3 -0.9291781270326211 1103 4 -0.8661077309824456 1103 5 -0.8482180534776589 1103 6 -0.7718874407731846 1103 7 -1.005758495849502 1103 8 -1.2165640038233292 1103 9 -0.6742466160910325 1103 10 -0.7931945359006463 1103 11 -0.6789227599080476 1103 12 -1.4333111997214587 1103 13 -0.6416881120754141 1103 14 -0.7064151410334638 1103 15 0.07747070259774702 1103 16 -0.18257510558090218 1103 17 -1.2121455724566772 1103 18 -1.416040619322164 1103 19 -0.4425479354289017 1103 20 -1.6800011074495984 1103 21 -1.11135686837493 1103 22 -1.465840864277844 1103 23 -1.8679045719561351 1103 24 -0.8475806968609589 1103 25 -0.6504669081179273 1103 26 -1.9730229961992158 1103 27 -1.794851490607904 1103 28 -0.5361020613618557 1103 29 -0.17044792562904476 1103 30 -0.22748777496392947 1103 31 -0.6409547195853165 1103 32 -0.9257885398597487 1103 33 -2.1214863174106338 1103 34 -1.24005988220366 1103 35 -1.9008824836965346 1103 36 -1.6661315100625824 1103 37 -0.6625400507050756 1103 38 -0.34603374361633854 1103 39 -0.8781459543360057 1103 40 -0.32327093962592457 1103 41 -1.0663262515869807 1103 42 -1.1870723192666226 1103 43 -1.58835747391457 1103 44 -0.4305105138117149 1103 45 -1.4888857059935798 1103 46 -1.9997230421815573 1103 47 -1.4374869578546097 1103 48 -1.9521771635570708 1103 49 -1.0155821981239725 1103 50 -1.3245882922350258 1104 1 0.5837334446208082 1104 2 1.484896830551687 1104 3 1.862157707536788 1104 4 1.2500688679181735 1104 5 1.4340435292812614 1104 6 1.5457549988045116 1104 7 1.5216387644244698 1104 8 1.033937697598887 1104 9 0.6303952127197192 1104 10 2.1451180022722873 1104 11 0.3685425572545036 1104 12 0.5299119047196896 1104 13 0.2727172626737371 1104 14 1.3501680541502026 1104 15 1.7959507654194908 1104 16 2.8455638002737373 1104 17 1.933784102091792 1104 18 2.223519628474712 1104 19 1.6169145896561687 1104 20 1.4449443597871188 1104 21 2.5182566033989104 1104 22 2.736497500611783 1104 23 2.401181895193325 1104 24 0.5999369717543459 1104 25 1.8108802692662458 1104 26 1.6289750842475144 1104 27 2.5479196462911418 1104 28 1.43641342594292 1104 29 1.0623851604890966 1104 30 1.6653226255246842 1104 31 2.138471159482826 1104 32 2.212419295407681 1104 33 1.4274622909132475 1104 34 -0.07153183055022794 1104 35 0.45707057450083255 1104 36 -0.09676583516510462 1104 37 0.6371283900941584 1104 38 0.16421841588108776 1104 39 0.24828280406203973 1104 40 -0.25416702199220964 1104 41 0.09404369867914042 1104 42 0.5714129787003941 1104 43 1.4266499425412356 1104 44 1.5923496205570125 1104 45 0.6760213701777857 1104 46 0.716357579055682 1104 47 0.7362762390741047 1104 48 0.28292448551369276 1104 49 0.11817576365946536 1104 50 0.7325620436867943 1105 1 -0.16181612258480982 1105 2 0.7853622886604583 1105 3 0.19867575424591521 1105 4 0.9861051246550652 1105 5 0.21597232235655284 1105 6 -0.368216910390327 1105 7 0.4511259439116486 1105 8 0.6151887183403524 1105 9 0.17032661889316358 1105 10 -0.6841323651946425 1105 11 -0.3942371259073454 1105 12 -0.9063529981057243 1105 13 -0.8575293484645954 1105 14 -0.9774899566288298 1105 15 0.8869192449229999 1105 16 5.123549038188457E-4 1105 17 -0.73655723385464 1105 18 0.12365028338243889 1105 19 -0.9225268606133064 1105 20 -0.6909775739055171 1105 21 -0.13146575492217139 1105 22 -0.18106741854549213 1105 23 -0.4492182354615828 1105 24 -0.3664602191651376 1105 25 -1.0523812038678386 1105 26 -1.6798196242287204 1105 27 -0.6601482942608747 1105 28 -0.029779616683246246 1105 29 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1106 15 -0.2284653704616133 1106 16 -0.7623081579166922 1106 17 -0.10835210564951225 1106 18 -0.16481125507910122 1106 19 0.0326013445149016 1106 20 0.07825319532525299 1106 21 0.25955988610346736 1106 22 -0.5144007518788278 1106 23 -0.2120387482820938 1106 24 -0.4177422704471887 1106 25 -0.5459709474575831 1106 26 -0.5003324447415416 1106 27 0.5898517057391253 1106 28 -0.5554206423242718 1106 29 -0.15789084800347197 1106 30 -0.3797533176404322 1106 31 0.4357927338744645 1106 32 0.7070706101226882 1106 33 0.8399643323961818 1106 34 0.08545820701305828 1106 35 1.8153099996192381 1106 36 0.10362612955806938 1106 37 -0.8190205565006501 1106 38 -0.8914324088780141 1106 39 -0.19953689216529824 1106 40 -0.5677388005321404 1106 41 0.4568560455679176 1106 42 0.30291714332557973 1106 43 -0.4650698026816933 1106 44 0.18364473732983555 1106 45 0.32574372849886757 1106 46 0.08317123249354097 1106 47 -0.7184844991261237 1106 48 -0.14966574803195698 1106 49 -0.3873770699188448 1106 50 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1.3291183993056563 2213 18 1.4963889966119481 2213 19 1.9476949421238352 2213 20 1.8512200035681339 2213 21 1.2246377530218275 2213 22 0.5760113922142978 2213 23 0.49305013011966636 2213 24 0.505138018997517 2213 25 0.5594246794104372 2213 26 1.119968011448485 2213 27 1.3255367773137685 2213 28 1.7821599774649393 2213 29 1.2291234669249533 2213 30 1.8873538083623984 2213 31 1.1366736525467935 2213 32 1.758470641584072 2213 33 1.0464689729498993 2213 34 0.8858750703133573 2213 35 0.9030085995877748 2213 36 1.6727625146453775 2213 37 1.8024990282091196 2213 38 1.2629249924119001 2213 39 1.353554188533142 2213 40 2.2213126388260456 2213 41 2.041329853968108 2213 42 2.1500148309140634 2213 43 2.1791019764594104 2213 44 1.5834402383523312 2213 45 2.0064500365057896 2213 46 2.1069245752652854 2213 47 2.0286446498435176 2213 48 1.546142022929189 2213 49 2.29730123745885 2213 50 2.0599818397088363 2214 1 -0.5116968620004343 2214 2 -0.44004021581181263 2214 3 0.03976102556374528 2214 4 -0.2808895086186354 2214 5 0.04617506534999194 2214 6 -0.6595108877308142 2214 7 -0.46673332859878514 2214 8 0.027383113092888323 2214 9 -1.0698343828957455 2214 10 -0.9046153705613662 2214 11 -0.16108268345057652 2214 12 -0.13369348707411888 2214 13 0.31211883281149255 2214 14 -0.04681017129788145 2214 15 -0.8893180836399104 2214 16 -0.7043176987634432 2214 17 -1.0859907460788492 2214 18 -0.34802532782805784 2214 19 -0.5558005636544507 2214 20 -0.6012339841619259 2214 21 -0.5204043357212171 2214 22 0.49290395891425476 2214 23 0.23802088201009397 2214 24 0.04975922031011493 2214 25 -0.5959113171653954 2214 26 -1.3443992306687917 2214 27 -0.05743511596629958 2214 28 -0.19793704065462236 2214 29 -1.028045886763163 2214 30 -0.8319000081592165 2214 31 -0.4704386316592277 2214 32 -0.4764416140872072 2214 33 0.15853916841287585 2214 34 -0.42244280670190903 2214 35 -0.9340936974588772 2214 36 -0.05558675058504292 2214 37 0.04055010539559589 2214 38 0.5783052178363555 2214 39 -0.25812365424043193 2214 40 -0.4358067790652367 2214 41 -0.7953800903891466 2214 42 0.45676329717173303 2214 43 0.33144182522528354 2214 44 0.501474194694179 2214 45 0.4123567279025657 2214 46 -0.715874682831446 2214 47 -0.14081604355068933 2214 48 -0.36672001159784706 2214 49 -0.6086054539379262 2214 50 -0.20392884655168686 2215 1 0.8894659286972413 2215 2 0.7247853655835357 2215 3 0.29659242091258164 2215 4 0.45341281696275715 2215 5 0.940651809536039 2215 6 0.9691228331994175 2215 7 1.3403887644244712 2215 8 0.6087784619300969 2215 9 0.5198458496623921 2215 10 1.383175327113051 2215 11 1.3871731305029087 2215 12 2.05598674548402 2215 13 2.4536886002533542 2215 14 1.8398177356788636 2215 15 1.3736179628717196 2215 16 1.9485892779807408 2215 17 1.419532509735106 2215 18 0.8031374628696177 2215 19 1.0697808316943878 2215 20 1.7050160158380727 2215 21 1.7749445014880827 2215 22 2.395374889146815 2215 23 1.7886022773589332 2215 24 0.43289875519383614 2215 25 1.9484200781834424 2215 26 1.5384496065405102 2215 27 1.8877683724057945 2215 28 2.0000280756244457 2215 29 1.8941924853298602 2215 30 2.0221697592826438 2215 31 2.1938055543872856 2215 32 1.821814199866278 2215 33 1.7271438195756659 2215 34 1.713022309577159 2215 35 1.5654702560294922 2215 36 0.8170876680196102 2215 37 1.7963640588839667 2215 38 0.5269970783014699 2215 39 0.9706554155270091 2215 40 1.1130304302370888 2215 41 1.3265675840294584 2215 42 1.1817632971717345 2215 43 0.953680197318306 2215 44 0.9503114039965048 2215 45 1.4005834720886128 2215 46 2.099677642749949 2215 47 1.7789513983097756 2215 48 1.4982509186347102 2215 49 1.2094178018760289 2215 50 1.796216502285521 2216 1 0.12476004634429927 2216 2 -0.6034009089262682 2216 3 0.08429666927859714 2216 4 0.16697490846602747 2216 5 0.1851779533268889 2216 6 0.6351767547680467 2216 7 0.23405706507806556 2216 8 -0.6412215380699031 2216 9 0.6167821241721967 2216 10 -0.24345865981505455 2216 11 0.34910123507807356 2216 12 0.4042302095363084 2216 13 0.2513193192076031 2216 14 1.1314844023455315 2216 15 1.4480052177736802 2216 16 1.163785356412113 2216 17 0.8991893724802011 2216 18 0.1037910576408585 2216 19 1.5737024003218387 2216 20 0.41347189819101615 2216 21 0.5873628021416799 2216 22 1.19688632705531 2216 23 0.38386371526742735 2216 24 0.20757195780821647 2216 25 0.12672890171285545 2216 26 0.21271431242286098 2216 27 -0.28759927465302937 2216 28 0.7507633697420957 2216 29 1.4777300670292064 2216 30 0.9520717200669573 2216 31 0.40990032562911694 2216 32 0.9618468796048418 2216 33 2.234006564673706 2216 34 1.849787015459512 2216 35 0.6959032625654409 2216 36 1.583264138607845 2216 37 0.6133575229362549 2216 38 1.1398647253602938 2216 39 0.43495280114792223 2216 40 0.9113964433089841 2216 41 1.4038143160556018 2216 42 1.0188548004397084 2216 43 1.1950609162725563 2216 44 1.8078277438657828 2216 45 1.600379223722598 2216 46 1.5326433290244594 2216 47 0.5743762349110817 2216 48 0.47941921928830666 2216 49 0.7687315273662243 2216 50 0.8137410120894444 >>END_MODULE >>Per sequence quality scores fail #Quality Count 3 2.0 4 3.0 5 5.0 6 26.0 7 660.0 8 3672.0 9 7930.0 10 7268.0 11 5796.0 12 5278.0 13 5520.0 14 5374.0 15 5279.0 16 4886.0 17 4708.0 18 4031.0 19 3725.0 20 3358.0 21 3178.0 22 2968.0 23 2781.0 24 2576.0 25 2533.0 26 2288.0 27 2141.0 28 2150.0 29 1864.0 30 1802.0 31 1695.0 32 1602.0 33 1405.0 34 1382.0 35 1157.0 36 1058.0 37 775.0 38 528.0 39 418.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 31.739352707538636 19.008808840300446 20.94360769200519 28.308230760155727 2 19.296195171777093 27.769156730637018 33.94979276748708 18.984855330098803 3 19.189369684351124 32.77582447801065 27.5529846202196 20.481821217418634 4 17.808703509099303 25.824731636892196 29.798371652213195 26.56819320179531 5 17.984678845020625 39.20644274209389 27.320762129247694 15.488116283637792 6 25.528864096167826 39.44236019720689 13.437174677378168 21.59160102924712 7 30.828308224155883 31.80452161615368 14.501777611910981 22.865392547779457 8 24.423012708452006 37.41922177918328 17.254620808862526 20.903144703502193 9 25.18881544701873 27.213977744821694 13.424802836405778 34.1724039717538 10 23.4114434994402 30.37064681503015 25.425742963210308 20.79216672231934 11 32.49523659863679 25.557367066726904 19.952267771906733 21.995128562729576 12 24.64519677460542 26.740131805101306 22.635709164481373 25.9789622558119 13 29.061818610407215 21.686604985561907 22.10305066100929 27.14852574302159 14 35.04267627906063 22.91750562305403 17.373027020125132 24.66679107776021 15 27.670880356739445 26.946007798764377 17.90376285470136 27.479348989794815 16 31.79023579291146 22.16964891743805 19.511691275563905 26.52842401408659 17 38.76322277115889 21.725123510750098 16.065728345103278 23.445925372987734 18 32.36153324087462 19.982885302015404 17.591745600834095 30.063835856275883 19 31.208981511546675 20.534089008275032 20.954236404246736 27.302693075931554 20 36.14526624597604 20.320144911842014 19.6448084741925 23.889780367989445 21 28.754232616741476 22.66024883849122 17.442318292778957 31.143200251988347 22 33.51805997720395 19.020948787485754 15.783319577093897 31.677671658216404 23 40.69548133595285 21.394891944990178 15.937131630648329 21.972495088408646 24 30.582023068527096 29.20145137024691 15.40556751919919 24.810958042026805 25 36.5706032051723 22.31141854912403 15.260432531221443 25.857545714482228 26 35.13287812546053 26.73674902981775 15.467898020336984 22.66247482438473 27 31.779419566541566 27.41536164109013 17.856090228518656 22.949128563849644 28 36.835280132821175 24.492342152056665 16.8973681366722 21.77500957844996 29 32.64069008881553 23.683486599072545 19.769315413031517 23.906507899080406 30 36.22369235911097 22.216870312300326 18.60433111502128 22.95510621356742 31 34.461650323265275 21.169453888026414 20.517027924617288 23.851867864091027 32 31.59792497691144 23.010944960798568 20.593032166787843 24.798097895502153 33 36.20464348526681 21.467522131719345 20.60190416302308 21.725930219990765 34 31.15727002967359 22.30726904710437 22.41928193841256 24.11617898480948 35 34.40883456799827 22.86258866990234 20.13813837417225 22.59043838792714 36 36.37962544461257 19.979562560182366 22.285652524220332 21.35515947098473 37 32.48450195015081 20.75608869501999 24.408814485150362 22.35059486967884 38 34.739934369534886 18.494429270401447 24.07400129688943 22.691635063174235 39 31.89522190671763 19.90823073975456 26.57869656209163 21.61785079143618 40 26.264534025851383 32.87886836254894 22.249109760176278 18.6074878514234 41 31.503915269058076 16.73298552775076 26.445996797044636 25.31710240614653 42 25.33725031686301 20.333271107006357 31.5811709684709 22.748307607659733 43 25.86505020336785 17.751950169964434 29.4500225964278 26.93297703023991 44 26.71159532768123 16.849230285585172 32.31916377675826 24.120010609975342 45 26.07794402255602 19.090096373942686 32.69149531883958 22.14046428466171 46 25.782654394357618 20.39763853007338 28.05669885364584 25.76300822192316 47 30.842829076620827 21.205304518664047 27.35068762278978 20.601178781925345 48 30.573592542019902 22.633279959134356 23.661797498943976 23.131329999901766 49 28.67007848491695 23.823462962781058 25.00417472962486 22.502283822677132 50 30.144602931352903 23.5834413925891 22.747455695705135 23.52449998035286 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 1.0 5 2.5 6 4.0 7 5.5 8 7.0 9 8.0 10 9.0 11 8.5 12 8.0 13 12.5 14 17.0 15 28.5 16 40.0 17 40.0 18 40.0 19 70.5 20 101.0 21 138.0 22 175.0 23 216.5 24 258.0 25 318.5 26 379.0 27 500.5 28 622.0 29 782.5 30 943.0 31 1097.5 32 1252.0 33 1497.0 34 1742.0 35 1975.5 36 2209.0 37 2499.5 38 2790.0 39 3089.0 40 3388.0 41 3735.5 42 4083.0 43 4413.5 44 4744.0 45 5035.0 46 5326.0 47 5726.5 48 6127.0 49 6447.0 50 6767.0 51 7027.0 52 7287.0 53 7341.0 54 7395.0 55 7225.5 56 7056.0 57 6660.0 58 6264.0 59 5899.0 60 5534.0 61 5156.0 62 4778.0 63 4415.0 64 4052.0 65 3717.5 66 3383.0 67 3157.5 68 2932.0 69 2707.0 70 2482.0 71 2334.5 72 2187.0 73 2034.0 74 1881.0 75 1705.5 76 1530.0 77 1370.0 78 1210.0 79 1078.5 80 947.0 81 822.5 82 698.0 83 578.5 84 459.0 85 396.5 86 334.0 87 250.0 88 166.0 89 124.5 90 83.0 91 72.0 92 61.0 93 40.5 94 20.0 95 18.5 96 17.0 97 16.5 98 16.0 99 17.0 100 18.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10410323898568088 2 0.0039284241126672035 3 0.0 4 9.821060281668009E-4 5 0.0019642120563336017 6 0.0 7 0.0 8 0.0 9 0.0029463180845004026 10 0.0 11 0.0039284241126672035 12 0.004910530140834004 13 0.007856848225334407 14 0.00883895425350121 15 0.01080316630983481 16 0.16204749464752216 17 0.00883895425350121 18 0.15222643436585415 19 0.18758225137985896 20 0.2386517648445326 21 0.22588438647836423 22 0.049105301408340046 23 0.02160633261966962 24 0.12276325352085012 25 0.04812319538017325 26 0.03633792304217163 27 0.03535581701400484 28 0.03044528687317083 29 0.037320029070338434 30 0.09133586061951249 31 0.04714108935200644 32 0.03928424112667203 33 0.04419477126750604 34 0.04714108935200644 35 0.03928424112667203 36 0.04714108935200644 37 0.03437371098583803 38 0.03928424112667203 39 0.04419477126750604 40 0.16106538861935535 41 0.04026634715483884 42 0.04223055921117244 43 0.03535581701400484 44 0.03044528687317083 45 0.03044528687317083 46 0.020624226591502818 47 0.02160633261966962 48 0.024552650704170023 49 0.018660014535169217 50 0.025534756732336825 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 101822.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 96.57441417375419 #Duplication Level Percentage of deduplicated Percentage of total 1 98.82339780747249 95.43811749916522 2 0.748469501901682 1.445660073461531 3 0.16474464579901155 0.4773035296890652 4 0.07525372709337563 0.290703384337373 5 0.04372851709479936 0.2111527960558622 6 0.03457603677263205 0.20034962974602738 7 0.020338845160371793 0.13749484394335212 8 0.015254133870278847 0.11785272338001611 9 0.009152480322167308 0.07955058828151088 >10 0.05796570870705961 0.9575533774626308 >50 0.005084711290092948 0.3565044882245487 >100 0.0020338845160371793 0.2877570662528727 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTATACACATCTGACGCTGCCGACGAGACCCAATGTGTAGATCTCGGTG 167 0.16401170670385573 No Hit ATACACATCTGACGCTGCCGACGAGACCCAATGTGTAGATCTCGGTGGTC 126 0.1237453595490169 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0039284241126672035 0.0 2 0.0 0.0 0.0 0.0039284241126672035 0.0 3 0.0 0.0 0.0 0.0039284241126672035 0.0 4 9.821060281668009E-4 0.0 0.0 0.007856848225334407 0.0 5 9.821060281668009E-4 0.0 0.0 0.007856848225334407 0.0 6 9.821060281668009E-4 0.0 0.0 0.007856848225334407 0.0 7 9.821060281668009E-4 0.0 0.0 0.007856848225334407 0.0 8 9.821060281668009E-4 0.0 0.0 0.01178527233800161 0.0 9 9.821060281668009E-4 0.0 0.0 0.013749484394335213 0.0 10 9.821060281668009E-4 0.0 0.0 0.018660014535169217 0.0 11 9.821060281668009E-4 0.0 0.0 0.026516862760503624 0.0 12 9.821060281668009E-4 0.0 0.0 0.026516862760503624 0.0 13 9.821060281668009E-4 0.0 0.0 0.028481074816837225 0.0 14 9.821060281668009E-4 0.0 0.0 0.028481074816837225 0.0 15 9.821060281668009E-4 0.0 0.0 0.029463180845004027 0.0 16 9.821060281668009E-4 0.0 0.0 0.029463180845004027 0.0 17 9.821060281668009E-4 0.0 0.0 0.04223055921117244 0.0 18 9.821060281668009E-4 0.0 0.0 0.04517687729567284 0.0 19 9.821060281668009E-4 0.0 0.0 0.05499793757734085 0.0 20 9.821060281668009E-4 0.0 0.0 0.057944255661841255 0.0 21 9.821060281668009E-4 0.0 0.0 0.06187267977450846 0.0 22 9.821060281668009E-4 0.0 0.0 0.08249690636601127 0.0 23 9.821060281668009E-4 0.0 0.0 0.08740743650684528 0.0 24 9.821060281668009E-4 0.0 0.0 0.09231796664767929 0.0 25 9.821060281668009E-4 0.0 0.0 0.09330007267584609 0.0 26 9.821060281668009E-4 0.0 0.0 0.09919270884484689 0.0 27 9.821060281668009E-4 0.0 0.0 0.1139242992673489 0.0 28 9.821060281668009E-4 0.0 0.0 0.12472746557718371 0.0 29 9.821060281668009E-4 0.0 0.0 0.14829801025318692 0.0 30 9.821060281668009E-4 0.0 0.0 0.15811907053485494 0.0 31 9.821060281668009E-4 0.0 0.0 0.17285066095735696 0.0 32 9.821060281668009E-4 0.0 0.0 0.19347488754885978 0.0 33 9.821060281668009E-4 0.0 0.0 0.2209738563375302 0.0 34 9.821060281668009E-4 0.0 0.0 0.26320441554870266 0.0 35 9.821060281668009E-4 0.0 0.0 0.32409498929504427 0.0 36 9.821060281668009E-4 0.0 0.0 0.4370371825342264 0.0 37 9.821060281668009E-4 0.0 0.0 0.5892636169000806 0.0 38 9.821060281668009E-4 0.0 0.0 0.7405079452377679 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGTGTA 40 4.0775194E-7 38.497417 34 ATTTCGG 40 4.09329E-7 38.4785 42 CTTTACA 150 0.0 36.700294 1 TTTACAC 150 0.0 36.64619 2 CGAACTT 25 0.0023378262 35.214954 25 GAACTTC 25 0.0023378262 35.214954 26 CTATACA 65 4.5292836E-10 33.877197 1 GTATATC 35 3.1985994E-4 31.426464 38 GACGAAT 35 3.1985994E-4 31.426464 23 TTACACA 190 0.0 31.230354 3 CTTATAC 785 0.0 30.295275 1 TTATACA 775 0.0 30.07352 2 GTAAGCA 30 0.005708749 29.345795 29 GTAGTTC 30 0.005722498 29.331367 38 ATCTCCG 30 0.0057362732 29.316952 42 ACATCTA 30 0.005750075 29.302555 8 CCTCTGA 30 0.005750075 29.302555 9 GGTGGTC 100 1.8189894E-12 28.584028 44 CTTAATA 85 2.5465852E-10 28.4967 1 TTAATAC 85 2.582965E-10 28.454689 2 >>END_MODULE