FastQCFastQC Report
Wed 25 May 2016
SRR1780466_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780466_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75502
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATCCT150.002217921269.9913946
TCCCGAC150.002217921269.9913919
GCATAAC200.00693040252.4935430
AGCGTTT200.00693040252.4935411
GTCCTAT200.00693040252.493543
GGCAAGA200.00693040252.4935412
CCGTGTA200.00693040252.4935469
TCTCGGC200.00693040252.493542
TAGATTC200.00693040252.4935438
AGGGGAC200.00693040252.4935446
CCCGACT200.00693040252.4935420
ACGGTGA350.00124908839.9950829
TCTGAAC501.6966484E-434.9956933
CTGTACC400.002406644934.9956934
CCCACGC400.002406644934.9956939
AGTCAGG450.004285836631.10728516
GTCAGGC450.004285836631.10728517
TCTGTAC450.004285836631.1072853
CAGCAGA500.007172751227.99655569
ATGTTGG500.007172751227.99655550
Produced by FastQC (version 0.11.5)