##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780466_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 75502 Sequences flagged as poor quality 0 Sequence length 76 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.47872904029032 34.0 23.0 34.0 12.0 34.0 2 29.12969192869063 34.0 27.0 34.0 11.0 34.0 3 29.203411830150195 34.0 27.0 34.0 11.0 34.0 4 29.40711504331011 34.0 31.0 34.0 11.0 34.0 5 29.591891605520384 34.0 31.0 34.0 11.0 34.0 6 32.283211040767135 37.0 31.0 38.0 11.0 38.0 7 31.99282138221504 37.0 28.0 38.0 11.0 38.0 8 31.369937220206086 37.0 27.0 38.0 11.0 38.0 9 31.780403168127997 37.0 27.0 38.0 12.0 38.0 10-11 31.957782575296015 37.0 31.0 38.0 11.0 38.0 12-13 32.13441365791635 37.0 31.0 38.0 11.0 38.0 14-15 31.827276098646394 37.0 28.0 38.0 11.0 38.0 16-17 31.631956769357103 37.0 27.0 38.0 11.0 38.0 18-19 31.671319965034037 37.0 29.0 38.0 11.0 38.0 20-21 31.74452332388546 37.0 27.5 38.0 11.0 38.0 22-23 31.59497761648698 37.0 27.0 38.0 11.0 38.0 24-25 31.940710179862783 37.0 31.0 38.0 11.0 38.0 26-27 31.89043336600355 37.0 31.0 38.0 11.0 38.0 28-29 31.943326004609148 37.0 31.0 38.0 11.0 38.0 30-31 31.857109745437207 37.0 31.0 38.0 11.0 38.0 32-33 31.59844772323912 37.0 27.0 38.0 11.0 38.0 34-35 31.628526396651743 37.0 27.0 38.0 11.0 38.0 36-37 31.611394400148342 37.0 27.0 38.0 11.0 38.0 38-39 31.914439352599928 37.0 31.0 38.0 11.0 38.0 40-41 31.625003311170566 37.0 27.0 38.0 11.0 38.0 42-43 31.876797965616806 37.0 29.0 38.0 11.0 38.0 44-45 31.042674366241954 37.0 26.5 38.0 11.0 38.0 46-47 31.677803237000344 37.0 27.0 38.0 11.0 38.0 48-49 31.55534290482371 37.0 27.0 38.0 11.0 38.0 50-51 31.47206034277238 37.0 27.0 38.0 11.0 38.0 52-53 31.4570739847951 37.0 27.0 38.0 11.0 38.0 54-55 31.23156340229398 37.0 27.0 38.0 11.0 38.0 56-57 31.23739106248841 37.0 26.5 38.0 11.0 38.0 58-59 31.135274562263252 37.0 26.5 38.0 11.0 38.0 60-61 31.220424624513257 37.0 26.5 38.0 11.0 38.0 62-63 31.2009880532966 37.0 26.5 38.0 11.0 38.0 64-65 31.300448994728615 37.0 27.0 38.0 11.0 38.0 66-67 31.31371354401208 37.0 27.0 38.0 11.0 38.0 68-69 30.80813091043946 37.0 26.0 38.0 11.0 38.0 70-71 30.758297793435936 36.5 25.0 38.0 11.0 38.0 72-73 30.822176895976266 37.0 25.0 38.0 11.0 38.0 74-75 30.507589202935023 36.0 25.0 38.0 11.0 38.0 76 29.869857752112527 35.0 24.0 37.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -2.946585392185881 1101 2 -0.49417888281486455 1101 3 0.3398995163298828 1101 4 -2.3756568514124723 1101 5 -0.49464264376321054 1101 6 -2.049715031105933 1101 7 -0.5965102425351958 1101 8 -0.3981562160467824 1101 9 -0.733412201837222 1101 10-11 -1.2062111470606318 1101 12-13 -1.466665272308262 1101 14-15 -1.1993344742873155 1101 16-17 -1.6423934588321671 1101 18-19 -0.2509370606659509 1101 20-21 -1.1059629766939487 1101 22-23 -0.10282264071176783 1101 24-25 -0.9294694587841725 1101 26-27 -1.0532376070403373 1101 28-29 -0.3630393644506569 1101 30-31 -1.6405826023275267 1101 32-33 0.27364701321011253 1101 34-35 -1.5872321756737442 1101 36-37 -0.004231876324983119 1101 38-39 -0.8670388183566047 1101 40-41 0.027184793527148088 1101 42-43 -1.0417358471269722 1101 44-45 -0.9279544896004737 1101 46-47 0.285830913978657 1101 48-49 -0.9119913027100033 1101 50-51 -1.2958308797416755 1101 52-53 0.24202866713269344 1101 54-55 -1.4021490265609806 1101 56-57 -1.0057888948260612 1101 58-59 -0.5332672302737826 1101 60-61 0.01875648209991354 1101 62-63 -1.3973700838713548 1101 64-65 -0.9585757321930188 1101 66-67 -1.8792194228998262 1101 68-69 -0.1217674944080791 1101 70-71 -0.19188279512195905 1101 72-73 -1.1534583004450312 1101 74-75 -2.1810834746665435 1101 76 -1.2053226381683437 1102 1 0.7933876210283373 1102 2 0.36953784899231934 1102 3 0.6116517962479904 1102 4 0.8589188046455973 1102 5 0.16574261586828243 1102 6 0.8900765192383844 1102 7 1.870198300178373 1102 8 1.63148271937105 1102 9 2.0305232159926696 1102 10-11 0.07354411070970457 1102 12-13 -3.019103388816731 1102 14-15 -0.8805530672521407 1102 16-17 -1.6382523830830529 1102 18-19 -0.17903821458368796 1102 20-21 0.8050387914058135 1102 22-23 0.49046788599584445 1102 24-25 0.6202583469109655 1102 26-27 -4.939986164643166 1102 28-29 0.10159864038835309 1102 30-31 0.5035058957182628 1102 32-33 -5.494231732369638 1102 34-35 -0.9539151274697595 1102 36-37 0.4025939193199015 1102 38-39 -0.04808292779267731 1102 40-41 -0.09297619660871703 1102 42-43 -0.01071267571435186 1102 44-45 -5.319402470244437 1102 46-47 -0.2387363668700324 1102 48-49 0.1329672865325051 1102 50-51 -6.0744112817517255 1102 52-53 -1.8508896280469074 1102 54-55 0.17560083385219727 1102 56-57 -1.4221647556114725 1102 58-59 0.8409883066701944 1102 60-61 0.7768408855988937 1102 62-63 -3.9305103220995328 1102 64-65 -0.24118088759967904 1102 66-67 -0.4555501506124635 1102 68-69 -7.372069932524585 1102 70-71 -0.9193395232068262 1102 72-73 0.01626764409247272 1102 74-75 -5.590433465360757 1102 76 -2.5221614619167134 1103 1 0.5078440129040835 1103 2 -0.6297154365512867 1103 3 0.2533782000711682 1103 4 0.2993309910255242 1103 5 0.09181788468548646 1103 6 -1.0545172680973423 1103 7 0.24519830017837307 1103 8 0.6473130658465536 1103 9 0.0786116269843049 1103 10-11 0.4640757714026549 1103 12-13 -0.8856655154595003 1103 14-15 -1.1386175833811727 1103 16-17 -0.989208177587237 1103 18-19 -0.87938170323363 1103 20-21 -0.6704689744245371 1103 22-23 -0.6563361761307966 1103 24-25 0.37765082002925254 1103 26-27 -0.6840124489920285 1103 28-29 0.12385073118882417 1103 30-31 -0.15800844119930701 1103 32-33 -5.231388243719696 1103 34-35 -1.4611582576967628 1103 36-37 -0.013400704336007152 1103 38-39 0.2821142048238059 1103 40-41 0.2760500877401455 1103 42-43 0.4789826647874378 1103 44-45 -4.194103784461877 1103 46-47 -0.5958152199166271 1103 48-49 -0.8756199297159988 1103 50-51 -0.8344919269130138 1103 52-53 -0.5127026507470234 1103 54-55 -0.17565364643454018 1103 56-57 0.2114971320826733 1103 58-59 0.1255611860011392 1103 60-61 0.02624351403377645 1103 62-63 0.07031106380249597 1103 64-65 0.17802460821872046 1103 66-67 -0.14947189493743096 1103 68-69 -2.631777220457682 1103 70-71 -0.5324667633501967 1103 72-73 0.320777799409079 1103 74-75 -1.4348928440943247 1103 76 -0.5948914499692819 1104 1 -1.5656723883481263 1104 2 -0.7653660422406858 1104 3 -0.39215739268778194 1104 4 -0.8880924471124914 1104 5 -0.43024894531315283 1104 6 -0.4575559233307729 1104 7 0.6523712959589645 1104 8 1.0561193813494825 1104 9 0.26792359105126806 1104 10-11 0.08473726589019748 1104 12-13 -0.5375047015218399 1104 14-15 -0.3354581305432802 1104 16-17 -0.5952378495621922 1104 18-19 -0.07393390140975598 1104 20-21 0.5172692180349685 1104 22-23 -0.549030473122766 1104 24-25 -0.8256804716544295 1104 26-27 0.01453797975439386 1104 28-29 -0.6820394280598414 1104 30-31 -0.5029111225658482 1104 32-33 -1.1837242311975764 1104 34-35 -0.2018347242817633 1104 36-37 -0.7572162072623705 1104 38-39 -1.0329633778629983 1104 40-41 -0.38241595282062946 1104 42-43 -0.09096935644571857 1104 44-45 -2.4782163474127437 1104 46-47 -0.12410908135667142 1104 48-49 0.03589157157704648 1104 50-51 0.3952617140288659 1104 52-53 -0.2757607016521568 1104 54-55 -0.8936678018721338 1104 56-57 -0.12507732007466643 1104 58-59 -0.04198168864157381 1104 60-61 -0.23121701407293216 1104 62-63 -0.1325438429621677 1104 64-65 -0.2269110798172136 1104 66-67 -0.228815504574019 1104 68-69 -3.632559852824631 1104 70-71 -0.7451130815753224 1104 72-73 -0.176389411697496 1104 74-75 -0.8813675956936216 1104 76 -0.9195477722777099 1105 1 -1.078711888119365 1105 2 -0.29924840714333456 1105 3 -0.29938122472479023 1105 4 0.2958331286081126 1105 5 0.25309201345864096 1105 6 0.5342086031294997 1105 7 1.43596135238721 1105 8 1.1489972218253115 1105 9 1.7654071963585771 1105 10-11 1.1281514288712344 1105 12-13 -0.5879342678885706 1105 14-15 0.07702725403300192 1105 16-17 0.8107659123207895 1105 18-19 0.6913042664515636 1105 20-21 0.6155865379475287 1105 22-23 0.48589403503645556 1105 24-25 0.39761778466197484 1105 26-27 0.7800049107695983 1105 28-29 -0.5280309892412802 1105 30-31 0.1918425757718083 1105 32-33 0.8924538990449129 1105 34-35 0.8006308341545143 1105 36-37 1.009521630163281 1105 38-39 0.3612209544027749 1105 40-41 0.8983781095046268 1105 42-43 0.6394713938974235 1105 44-45 1.5687055172611402 1105 46-47 0.9714376625811099 1105 48-49 0.7359068224539698 1105 50-51 0.4740324933486768 1105 52-53 0.192059948035201 1105 54-55 -0.5134888582495414 1105 56-57 0.3116315406848216 1105 58-59 0.37317259939665703 1105 60-61 1.2197514166118282 1105 62-63 1.1428107399263503 1105 64-65 0.8381084921415081 1105 66-67 0.8457104473348842 1105 68-69 0.8748622406124085 1105 70-71 1.0182441447985262 1105 72-73 0.5204231550250924 1105 74-75 -0.5780784899033691 1105 76 -0.5686250942237194 1106 1 -0.41373422043929864 1106 2 0.18928516640318804 1106 3 -0.3369896021597647 1106 4 -0.18845918081650126 1106 5 0.24440305200535306 1106 6 -0.07392486532375386 1106 7 0.09099269270173949 1106 8 0.5573221101637102 1106 9 0.4692130740750464 1106 10-11 0.34928831285577644 1106 12-13 0.06483569044945625 1106 14-15 0.49853571779459216 1106 16-17 0.6155551627805664 1106 18-19 0.1863780194073108 1106 20-21 0.31436295201501707 1106 22-23 0.5066499559162345 1106 24-25 0.6484904341383881 1106 26-27 0.15537555221387578 1106 28-29 -0.7554453194593442 1106 30-31 0.08573590610247805 1106 32-33 0.5025015663496397 1106 34-35 0.6986246786915409 1106 36-37 0.22930731495853607 1106 38-39 0.407264943443046 1106 40-41 -0.009599595708753128 1106 42-43 0.6159098580343496 1106 44-45 1.0164403819535544 1106 46-47 0.708563736972124 1106 48-49 0.28706472509256287 1106 50-51 0.623602736939862 1106 52-53 0.13439939929819644 1106 54-55 0.28749076116268313 1106 56-57 0.3716003880368497 1106 58-59 0.09155078503641434 1106 60-61 0.43167387279169134 1106 62-63 0.3727304186412077 1106 64-65 -0.3302471735117578 1106 66-67 0.7630808635792938 1106 68-69 1.1569626046855213 1106 70-71 0.34221291708393053 1106 72-73 -0.12640715687672355 1106 74-75 -1.0904550991440694 1106 76 0.47346851385344735 1107 1 -0.2539770513363351 1107 2 0.31469454414901676 1107 3 0.6590164686126627 1107 4 0.1206709413977336 1107 5 0.08240928253494673 1107 6 0.5553186844805467 1107 7 0.4033179582980324 1107 8 0.38718072506905443 1107 9 0.05224686271631285 1107 10-11 -0.10202568986009908 1107 12-13 0.38830401859067365 1107 14-15 -0.5904889646880385 1107 16-17 0.1081863633556921 1107 18-19 0.8026213295758531 1107 20-21 0.8458151446821631 1107 22-23 0.03676418229214207 1107 24-25 0.21908313323575612 1107 26-27 0.6939169692599663 1107 28-29 -0.446834408044694 1107 30-31 -0.45963512992276634 1107 32-33 0.5796642790263782 1107 34-35 -0.15815301920764924 1107 36-37 -0.15189326016727378 1107 38-39 0.4839683542586002 1107 40-41 0.314787336154815 1107 42-43 0.38298390548222727 1107 44-45 0.3479764471344815 1107 46-47 -0.050702178835841494 1107 48-49 0.1317208477860703 1107 50-51 0.5885160686756272 1107 52-53 0.47532119816391827 1107 54-55 0.3636350218863882 1107 56-57 0.2731700022232886 1107 58-59 0.46242705310894294 1107 60-61 0.049454845712851636 1107 62-63 0.16332870923949727 1107 64-65 0.5624159784971852 1107 66-67 0.8432390231767108 1107 68-69 0.5492263637717123 1107 70-71 -0.05929678816409378 1107 72-73 0.41494998027480534 1107 74-75 -0.5945289069561959 1107 76 -0.6015774163326704 1108 1 -0.5113803211877368 1108 2 -0.2687363172901449 1108 3 -0.5455443002634581 1108 4 -0.7874043857647308 1108 5 0.17438715252111692 1108 6 -0.08833134921098207 1108 7 0.5169825325435156 1108 8 1.263763340240878 1108 9 -0.7557646278677908 1108 10-11 -0.13751705418963667 1108 12-13 -0.10950603212393872 1108 14-15 0.3536274306731677 1108 16-17 -1.0052033589551925 1108 18-19 -0.5150897744422984 1108 20-21 0.497187631116887 1108 22-23 -0.5584409813777533 1108 24-25 -1.1007824553020313 1108 26-27 -0.84700937042156 1108 28-29 0.07293719886384054 1108 30-31 0.062778602034534 1108 32-33 0.20281567811138146 1108 34-35 -0.06613081826428768 1108 36-37 -0.40117509866072965 1108 38-39 0.3884085429311597 1108 40-41 0.08220053614062905 1108 42-43 0.4012571260376063 1108 44-45 0.19354512465336526 1108 46-47 0.28293260500640827 1108 48-49 -0.014172332339477123 1108 50-51 0.4694165190781483 1108 52-53 0.022976977147500577 1108 54-55 0.2552458315469899 1108 56-57 0.5765001437276993 1108 58-59 -0.9619354677266081 1108 60-61 0.6204996377120011 1108 62-63 -0.3108795398650166 1108 64-65 0.22978307428751776 1108 66-67 0.17375290763250106 1108 68-69 0.5670917395209045 1108 70-71 1.2314788262629754 1108 72-73 0.281434003397834 1108 74-75 0.14424649468227457 1108 76 0.18194565349745417 1109 1 0.3656287396980282 1109 2 0.0892279472538462 1109 3 0.05301611975517062 1109 4 0.5140775348043256 1109 5 0.7837698267526356 1109 6 0.42802058575387747 1109 7 -0.5037812916583633 1109 8 0.9095250474133749 1109 9 0.9682594215860156 1109 10-11 1.3699348892433392 1109 12-13 1.2514559460283152 1109 14-15 0.5732734633601062 1109 16-17 -0.21198632110270665 1109 18-19 0.7295880136853974 1109 20-21 1.3920474837716412 1109 22-23 1.5242925269633396 1109 24-25 1.4351392992919259 1109 26-27 1.1256978867551766 1109 28-29 0.6297921400104229 1109 30-31 0.59571111113776 1109 32-33 1.2971420393612796 1109 34-35 0.3182031642913934 1109 36-37 0.9840565043539193 1109 38-39 1.2286744418218376 1109 40-41 1.0969519968909012 1109 42-43 0.9896558050112674 1109 44-45 0.4556164262030329 1109 46-47 0.05015252201885545 1109 48-49 0.4884189116269866 1109 50-51 1.4023744325339855 1109 52-53 1.560939548067985 1109 54-55 0.9135826932626294 1109 56-57 1.7368016087120353 1109 58-59 1.3012112847503197 1109 60-61 1.6709357457651777 1109 62-63 0.6655875614983522 1109 64-65 0.7144219090745452 1109 66-67 1.2162374533179978 1109 68-69 1.8255302186469926 1109 70-71 0.8863861003759332 1109 72-73 0.7038999192247459 1109 74-75 1.9098405331275323 1109 76 -0.09130356320167365 1110 1 0.2031724058131239 1110 2 0.6340523635068358 1110 3 0.16132647092266694 1110 4 0.08151640224319223 1110 5 0.2220489221745865 1110 6 -0.7005140713522096 1110 7 0.6686217236017953 1110 8 1.6612624991508298 1110 9 -0.07145376098430845 1110 10-11 -0.12477921261362468 1110 12-13 0.16608179636845932 1110 14-15 0.23712711292803945 1110 16-17 -0.2686327634634367 1110 18-19 0.7326860096405348 1110 20-21 -0.6633671145000974 1110 22-23 0.38961703514499746 1110 24-25 -0.028953364800742065 1110 26-27 -0.5603565350135327 1110 28-29 0.19268973147944735 1110 30-31 0.11705694676930989 1110 32-33 1.0107107100728037 1110 34-35 0.9810823700277389 1110 36-37 0.0906569823829706 1110 38-39 0.3445597148499715 1110 40-41 0.2785202998877807 1110 42-43 0.4744599969583163 1110 44-45 1.7161330652910998 1110 46-47 0.8123146841810183 1110 48-49 1.097244063309283 1110 50-51 0.5849355650951189 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0.13574580539911096 2116 40-41 0.04464470454721692 2116 42-43 -0.2567760161248316 2116 44-45 0.9970047012582732 2116 46-47 0.6087512481335011 2116 48-49 0.5951783032817417 2116 50-51 0.2492352150635604 2116 52-53 0.699299095708696 2116 54-55 0.24477988551532448 2116 56-57 0.4475126447187918 2116 58-59 -0.35295484029842683 2116 60-61 -0.6820084892536684 2116 62-63 0.09406799825904244 2116 64-65 0.739548647668073 2116 66-67 -0.358622809258371 2116 68-69 0.2889645095608131 2116 70-71 -1.4054506343179405 2116 72-73 0.3159108382420328 2116 74-75 -0.09130336392173533 2116 76 -1.0927854298336754 2117 1 -0.7324948050218723 2117 2 -0.17563987907131207 2117 3 -0.5515038090217104 2117 4 -0.9407642676884826 2117 5 -1.0663602924091222 2117 6 -0.6295605675128009 2117 7 -1.2615131092175993 2117 8 -0.5138690081954458 2117 9 0.7618493407751679 2117 10-11 0.2518003871203405 2117 12-13 0.2527708343997688 2117 14-15 0.00996706697604921 2117 16-17 -0.3728409985441523 2117 18-19 -0.5436759227616648 2117 20-21 -0.14734438035655728 2117 22-23 -0.043027018304428566 2117 24-25 0.4400290404232763 2117 26-27 0.23886046722360277 2117 28-29 -0.15107349483412236 2117 30-31 -0.4779911214331207 2117 32-33 0.7898690630566598 2117 34-35 0.28472829783473586 2117 36-37 -0.4131219643489956 2117 38-39 -0.3122514587397305 2117 40-41 -0.358232224448642 2117 42-43 -0.46871975997982673 2117 44-45 0.205143888194538 2117 46-47 -0.16528371959187993 2117 48-49 0.216689011613294 2117 50-51 0.622316906167736 2117 52-53 0.5067414930070342 2117 54-55 -0.5818562876988835 2117 56-57 0.02080380019265249 2117 58-59 -0.19906265032706827 2117 60-61 0.900986423754496 2117 62-63 -0.2329742793453704 2117 64-65 -0.06847084534265946 2117 66-67 -0.3960485349027927 2117 68-69 0.6288311363295023 2117 70-71 0.8898513791048792 2117 72-73 0.730457924977383 2117 74-75 0.7138947722728517 2117 76 1.1803304595349395 2118 1 1.3678334171894626 2118 2 1.3427062728906698 2118 3 1.2075223017900285 2118 4 0.9904070447104765 2118 5 0.7819226645300823 2118 6 0.925085981255144 2118 7 1.745198300178373 2118 8 -1.6158138472685835 2118 9 -0.1428682411579132 2118 10-11 0.05249112341583384 2118 12-13 1.5559844727674772 2118 14-15 1.1664359838427494 2118 16-17 0.8174788956409245 2118 18-19 0.19587730312379747 2118 20-21 -0.7528653718815654 2118 22-23 0.5181104921056026 2118 24-25 1.4005199040885898 2118 26-27 1.432952467419554 2118 28-29 0.7359592783986244 2118 30-31 1.0351867554100664 2118 32-33 1.6345023849593652 2118 34-35 1.5308949563366703 2118 36-37 0.5665233597091976 2118 38-39 0.6840744120288988 2118 40-41 0.6773333465110412 2118 42-43 1.0428659941964327 2118 44-45 1.2344634394608924 2118 46-47 0.007816755595499103 2118 48-49 0.34709610513213107 2118 50-51 1.3311544116789342 2118 52-53 0.3490968547862927 2118 54-55 1.7143275167394627 2118 56-57 1.9150378667188903 2118 58-59 1.7333578660105537 2118 60-61 -0.40342743016212523 2118 62-63 1.619080783604712 2118 64-65 0.5827073254308672 2118 66-67 -1.0534482311747801 2118 68-69 1.988601870665466 2118 70-71 1.3828705335652707 2118 72-73 0.23320127480347708 2118 74-75 1.5140711304759584 2118 76 1.142511422646681 2119 1 0.18875799139870963 2119 2 1.0065748860293589 2119 3 1.4162814258776208 2119 4 0.5866965824233752 2119 5 1.349075949201616 2119 6 1.4715580007198596 2119 7 -0.7964683664882948 2119 8 1.7166679045562319 2119 9 1.2907084011778558 2119 10-11 1.2939144810800727 2119 12-13 1.611915129701785 2119 14-15 1.3111135994145222 2119 16-17 1.2751735428428717 2119 18-19 1.1130914150459397 2119 20-21 0.6323536062206259 2119 22-23 2.025653071181029 2119 24-25 1.9327583469109726 2119 26-27 2.143810131653133 2119 28-29 1.5678023440920583 2119 30-31 1.2298947846071542 2119 32-33 1.984471971334063 2119 34-35 2.361825613270984 2119 36-37 1.7859272526532415 2119 38-39 1.8213615166517698 2119 40-41 1.2857275070949825 2119 42-43 2.1323428798411967 2119 44-45 1.0704123445703821 2119 46-47 1.4668191886855197 2119 48-49 1.415374693939917 2119 50-51 1.6589220515816088 2119 52-53 1.3148511126938374 2119 54-55 1.5783786116299758 2119 56-57 2.5657982073514916 2119 58-59 2.7558031214850125 2119 60-61 1.5638779226359318 2119 62-63 1.539397085307872 2119 64-65 1.647245038326247 2119 66-67 1.7925979975356832 2119 68-69 1.2922819193272694 2119 70-71 1.815382699015391 2119 72-73 0.6449713477961794 2119 74-75 1.3035480161207254 2119 76 2.4287644640092125 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 3.0 12 60.0 13 392.0 14 936.0 15 1511.0 16 1925.0 17 2136.0 18 2282.0 19 2164.0 20 1746.0 21 1435.0 22 1094.0 23 861.0 24 814.0 25 758.0 26 813.0 27 942.0 28 1018.0 29 1211.0 30 1601.0 31 2012.0 32 2586.0 33 3479.0 34 5141.0 35 7943.0 36 13804.0 37 16835.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 35.66485780189598 26.830308929214276 9.138544819402409 28.366288449487342 2 21.914941523953324 24.3665646812625 29.25391716666004 24.464576628124128 3 20.46038515536012 24.473523880162116 27.54364122804694 27.522449736430822 4 16.78101242351196 19.714709544118037 32.82429604513788 30.679981987232125 5 12.846017324044395 33.50772164975762 34.22690789647957 19.419353129718417 6 23.50931101162883 34.90636009642129 17.966411485788456 23.617917406161425 7 23.105348202696618 35.10370586209637 18.912081799157637 22.878864136049376 8 20.535879844242537 38.11422213981086 18.25382109083203 23.096076925114566 9 21.125268204815768 22.904029032343516 18.493549839739345 37.47715292310138 10-11 21.048449047707347 27.307223649704643 25.909909671266984 25.734417631321026 12-13 20.90143307462054 25.029800535085162 25.829117109480542 28.239649280813754 14-15 20.196153744271676 26.646976239040026 22.72257688538052 30.434293131307783 16-17 21.2775820508066 28.46348441100898 24.151015867129345 26.10791767105507 18-19 19.805435617599535 28.06018383618977 24.525840375089402 27.608540171121295 20-21 19.45445153770761 27.929061481815054 25.075494688882415 27.540992291594925 22-23 21.602739000291383 27.631056130963415 24.40531376652274 26.360891102222457 24-25 20.471643135281184 29.220418002172128 24.239755238271833 26.068183624274855 26-27 19.318031310428864 29.723053693941882 25.688723477523773 25.270191518105477 28-29 20.116023416598235 28.022436491748564 27.32709067309475 24.53444941855845 30-31 20.382241530025695 27.913830097215968 26.93637254642261 24.767555826335727 32-33 18.799501999947022 29.77735689120818 26.26288045349792 25.160260655346878 34-35 19.716034012344043 28.578050912558606 25.942359142804168 25.763555932293187 36-37 20.13853937644036 27.87012264575773 26.739026780747533 25.252311197054382 38-39 19.426637704961458 27.13173160975868 27.968133294482268 25.473497390797593 40-41 19.19882917008821 27.134380546210696 27.299939074461605 26.36685120923949 42-43 18.404148234483856 26.50393367063124 28.09064660538794 27.00127148949697 44-45 18.300177478742285 26.950941696908693 28.64890996265 26.099970861699028 46-47 18.01276787369871 27.45953749569548 28.16415459193134 26.363540038674472 48-49 17.443246536515588 28.02773436465259 27.73171571613997 26.79730338269185 50-51 17.502847606685915 27.88005615745278 27.243649174856294 27.373447061005006 52-53 17.181001827766153 27.634367301528435 27.476755582633572 27.707875288071836 54-55 16.874387433445474 27.90522105374692 26.841673068263095 28.378718444544514 56-57 17.41609493788244 27.880718391565786 26.919154459484517 27.784032211067256 58-59 17.495563031442877 27.436359301740353 26.654260814283067 28.413816852533706 60-61 17.228020449789412 26.662207623639112 26.933723609970595 29.176048316600884 62-63 17.423379513125482 27.099944372334505 26.415194299488753 29.061481815051255 64-65 17.91806839553919 27.705226351619828 25.150989377764827 29.22571587507616 66-67 17.473047071600753 29.18333289184393 24.213265873751688 29.130354162803634 68-69 17.4273529178035 28.629042939259886 25.125162247357686 28.81844189557892 70-71 18.10945405419724 29.13300309925565 24.6536515588991 28.103891287648008 72-73 17.89489020158406 29.15684352732378 24.714577097295436 28.233689173796723 74-75 17.796217318746525 29.482662710921563 24.289422796747107 28.43169717358481 76 17.69754443590898 28.734338163227463 24.141082355434293 29.42703504542926 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 1.0 9 0.5 10 0.0 11 1.0 12 2.0 13 2.5 14 3.5 15 4.5 16 8.0 17 11.0 18 14.5 19 22.5 20 35.5 21 44.0 22 48.5 23 64.0 24 110.0 25 145.0 26 182.5 27 268.5 28 381.0 29 445.0 30 535.0 31 720.5 32 978.0 33 1140.0 34 1299.5 35 1648.0 36 2055.5 37 2274.0 38 2477.0 39 3033.5 40 3765.5 41 4306.5 42 4469.0 43 4671.0 44 5044.5 45 5303.5 46 5391.0 47 5373.5 48 5165.0 49 4751.0 50 4528.0 51 4345.5 52 3821.5 53 3128.0 54 2776.0 55 2662.5 56 2260.0 57 1822.0 58 1673.0 59 1461.5 60 1064.0 61 784.5 62 691.0 63 584.0 64 403.0 65 277.0 66 181.0 67 137.0 68 114.0 69 72.5 70 47.0 71 40.0 72 34.5 73 28.5 74 23.5 75 19.0 76 16.5 77 12.0 78 10.0 79 10.0 80 9.0 81 6.0 82 4.5 83 5.0 84 4.5 85 5.0 86 4.5 87 3.0 88 3.0 89 4.0 90 3.5 91 4.0 92 6.0 93 4.0 94 4.0 95 5.5 96 5.0 97 3.5 98 3.0 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.6635585812296363 2 0.0013244682260072581 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 75502.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.75706603798575 #Duplication Level Percentage of deduplicated Percentage of total 1 80.99133332085431 57.30709120288204 2 10.179136327050147 14.404916426054939 3 4.121820189805889 8.749437101003947 4 1.8699810942852328 5.292575031125003 5 1.0875465623420624 3.847580196551085 6 0.5896336783782266 2.5032449471537177 7 0.3519083540797035 1.7430001854255517 8 0.20403197124833874 1.1549362930783291 9 0.18531344177601408 1.1801011893724669 >10 0.4192950601800723 3.817117427352918 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0026489364520145163 0.0 2 0.0 0.0 0.0 0.0026489364520145163 0.0 3 0.0 0.0 0.0 0.0026489364520145163 0.0 4 0.0 0.0 0.0 0.003973404678021774 0.0 5 0.0 0.0 0.0 0.0052978729040290325 0.0 6 0.0 0.0 0.0 0.0052978729040290325 0.0 7 0.0 0.0 0.0 0.0052978729040290325 0.0 8 0.0 0.0 0.0 0.0052978729040290325 0.0 9 0.0 0.0 0.0 0.01324468226007258 0.0 10 0.0 0.0 0.0 0.01324468226007258 0.0 11 0.0 0.0 0.0 0.014569150486079839 0.0 12 0.0 0.0 0.0 0.014569150486079839 0.0 13 0.0 0.0 0.0 0.017218086938094356 0.0 14 0.0 0.0 0.0 0.01986702339010887 0.0 15 0.0 0.0 0.0 0.02119149161611613 0.0 16 0.0 0.0 0.0 0.02119149161611613 0.0 17 0.0 0.0 0.0 0.022515959842123387 0.0 18 0.0 0.0 0.0 0.023840428068130647 0.0 19 0.0 0.0 0.0 0.030462769198166935 0.0 20 0.0 0.0 0.0 0.033111705650181456 0.0 21 0.0 0.0 0.0 0.033111705650181456 0.0 22 0.0 0.0 0.0 0.03443617387618871 0.0 23 0.0 0.0 0.0 0.037085110328203226 0.0 24 0.0 0.0 0.0 0.04238298323223226 0.0 25 0.0 0.0 0.0 0.04238298323223226 0.0 26 0.0 0.0 0.0 0.051654260814283065 0.0 27 0.0 0.0 0.0 0.07417022065640645 0.0 28 0.0 0.0 0.0 0.09271277582050806 0.0 29 0.0 0.0 0.0 0.12185107679266774 0.0 30 0.0 0.0 0.0 0.16026065534687822 0.0 31 0.0 0.0 0.0 0.20396810680511773 0.0 32 0.0 0.0 0.0 0.25164896294137906 0.0 33 0.0 0.0 0.0 0.30727662843368386 0.0 34 0.0 0.0 0.0 0.37217557150803954 0.0 35 0.0 0.0 0.0 0.4701862202325766 0.0 36 0.0 0.0 0.0 0.6039575110593097 0.0 37 0.0 0.0 0.0 0.8715000927127758 0.0 38 0.0 0.0 0.0 1.1734788482424308 0.0 39 0.0 0.0 0.0 1.5284363328123758 0.0 40 0.0 0.0 0.0 1.9972980848189452 0.0 41 0.0 0.0 0.0 2.585361977166168 0.0 42 0.0 0.0 0.0 3.254218431299833 0.0 43 0.0 0.0 0.0 3.924399353659506 0.0 44 0.0 0.0 0.0 4.544250483430902 0.0 45 0.0 0.0 0.0 5.275356944186909 0.0 46 0.0 0.0 0.0 5.961431485258669 0.0 47 0.0 0.0 0.0 6.520357076633732 0.0 48 0.0 0.0 0.0 7.107096500754947 0.0 49 0.0 0.0 0.0 7.6289369818018065 0.0 50 0.0 0.0 0.0 8.195809382532913 0.0 51 0.0 0.0 0.0 8.746788164551932 0.0 52 0.0 0.0 0.0 9.357368016741278 0.0 53 0.0 0.0 0.0 10.040793621361024 0.0 54 0.0 0.0 0.0 10.73746390824084 0.0 55 0.0 0.0 0.0 11.32287886413605 0.0 56 0.0 0.0 0.0 11.94140552568144 0.0 57 0.0 0.0 0.0 12.424836428174087 0.0 58 0.0 0.0 0.0 13.035416280363433 0.0 59 0.0 0.0 0.0 13.495006754787953 0.0 60 0.0 0.0 0.0 14.069825964875102 0.0 61 0.0 0.0 0.0 14.561203676723796 0.0 62 0.0 0.0 0.0 15.117480331646844 0.0 63 0.0 0.0 0.0 15.611506979947551 0.0 64 0.0 0.0 0.0 16.17705491245265 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAATCCT 15 0.0022179212 69.99139 46 TCCCGAC 15 0.0022179212 69.99139 19 GCATAAC 20 0.006930402 52.49354 30 AGCGTTT 20 0.006930402 52.49354 11 GTCCTAT 20 0.006930402 52.49354 3 GGCAAGA 20 0.006930402 52.49354 12 CCGTGTA 20 0.006930402 52.49354 69 TCTCGGC 20 0.006930402 52.49354 2 TAGATTC 20 0.006930402 52.49354 38 AGGGGAC 20 0.006930402 52.49354 46 CCCGACT 20 0.006930402 52.49354 20 ACGGTGA 35 0.001249088 39.99508 29 TCTGAAC 50 1.6966484E-4 34.995693 3 CTGTACC 40 0.0024066449 34.995693 4 CCCACGC 40 0.0024066449 34.995693 9 AGTCAGG 45 0.0042858366 31.107285 16 GTCAGGC 45 0.0042858366 31.107285 17 TCTGTAC 45 0.0042858366 31.107285 3 CAGCAGA 50 0.0071727512 27.996555 69 ATGTTGG 50 0.0071727512 27.996555 50 >>END_MODULE