FastQCFastQC Report
Wed 25 May 2016
SRR1780466_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780466_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75502
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTCTT37124.916426054938942RNA PCR Primer, Index 27 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGT29363.8886387115573093No Hit
CTTTACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTC12231.6198246404068768No Hit
CTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTC4720.6251490026754258No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCG3000.3973404678021774No Hit
TATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTCT2700.35760642102195966No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCC1740.2304574713252629No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCC1420.18807448809303065No Hit
CTTATAACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCG1300.17218086938094354No Hit
CTTAATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCG1260.1668829964769145No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGACCCTATATCGTATGCCGT1250.16555852825090725No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCC1150.15231384599083467No Hit
ATACACATCTCCGAGCCCACGAGACTTGACCCTAACTCGTATGCCGTCTT1080.14304256840878388No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGACCCTACCTCGTATGCCGT900.11920214034065323No Hit
CTTAACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTC880.11655320388863871No Hit
ATACACATCTCCGAGCCCACGAGACTTGACCCTATATCGTATGCCGTCTT860.1139042674366242No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGACCCTAACTCGTATGCCGT780.10330852162856613No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATATG150.0022168470.01
CCTTGAA150.0022168470.056
AGTTTGG200.006927030652.5000048
AGCCTTG200.006927030652.50000422
GAACAGT200.006927030652.50000413
TATAACA200.006927030652.5000043
CTTTACA2100.051.6666681
TTTACAC2150.048.837212
TTACACA2250.046.6666683
ACATAGA305.844378E-446.6666641
AAAAGTA350.001248332440.00000431
CCTCTAG350.001248332440.0000041
GTGAGTA459.120466E-538.8888961
CTATACA753.4677214E-837.3333361
CTTGGGC501.6954217E-435.00000447
GTTGTTG501.6954217E-435.00000421
TATTAAG501.6954217E-435.00000415
TCTTTAT400.002405192735.0000041
CTTTATA400.002405192735.0000042
TTTGATA501.6954217E-435.00000437