##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780466_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 75502 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.85066620751768 34.0 34.0 34.0 31.0 34.0 2 33.22888135413631 34.0 34.0 34.0 32.0 34.0 3 33.42747211994384 34.0 34.0 34.0 34.0 34.0 4 33.45033244152473 34.0 34.0 34.0 34.0 34.0 5 33.46936504993245 34.0 34.0 34.0 34.0 34.0 6 37.15977060210326 38.0 37.0 38.0 36.0 38.0 7 36.713742682313054 38.0 37.0 38.0 34.0 38.0 8 36.73098725861566 38.0 37.0 38.0 34.0 38.0 9 36.50499324521205 38.0 37.0 38.0 34.0 38.0 10-11 36.19453789303594 38.0 36.5 38.0 33.0 38.0 12-13 36.048151042356494 38.0 37.0 38.0 32.0 38.0 14-15 36.22953696590819 38.0 37.0 38.0 33.5 38.0 16-17 36.3752549601335 38.0 37.0 38.0 34.0 38.0 18-19 36.424982119678944 38.0 37.0 38.0 34.0 38.0 20-21 36.1401353606527 38.0 37.0 38.0 33.0 38.0 22-23 36.156572011337445 38.0 36.5 38.0 32.5 38.0 24-25 35.31258774601997 37.5 35.5 38.0 29.0 38.0 26-27 35.64167836613599 38.0 36.0 38.0 31.0 38.0 28-29 35.44332600460915 38.0 36.0 38.0 31.0 38.0 30-31 35.074468226007255 37.0 35.5 38.0 29.0 38.0 32-33 35.61703001245 38.0 36.0 38.0 31.0 38.0 34-35 35.4201213212895 38.0 36.0 38.0 31.0 38.0 36-37 35.51443670366348 38.0 36.0 38.0 31.0 38.0 38-39 35.04191941935313 37.5 35.5 38.0 29.0 38.0 40-41 34.78162830123705 37.0 34.5 38.0 27.0 38.0 42-43 35.391969749145716 37.5 36.0 38.0 29.0 38.0 44-45 35.641605520383564 38.0 36.0 38.0 31.5 38.0 46-47 35.83683213689704 38.0 37.0 38.0 32.0 38.0 48-49 35.555217080342246 38.0 36.5 38.0 31.0 38.0 50-51 35.710855341580356 38.0 36.0 38.0 31.0 38.0 52-53 35.60630844216047 38.0 36.0 38.0 31.0 38.0 54-55 35.81149505973352 38.0 37.0 38.0 32.0 38.0 56-57 35.8308058064687 38.0 37.0 38.0 32.0 38.0 58-59 35.85922227225769 38.0 37.0 38.0 32.0 38.0 60-61 35.54143598845064 38.0 36.5 38.0 29.5 38.0 62-63 35.93114089692988 38.0 36.5 38.0 32.5 38.0 64-65 35.953835659982516 38.0 37.0 38.0 33.0 38.0 66-67 34.98145082249477 38.0 35.5 38.0 27.5 38.0 68-69 33.58671955709782 37.0 34.0 38.0 22.0 38.0 70-71 32.82319673651029 37.0 32.0 38.0 16.0 38.0 72-73 32.02719133267993 37.0 29.0 38.0 11.0 38.0 74-75 32.47392122062992 37.0 31.5 38.0 11.0 38.0 76 32.10232841514132 37.0 31.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6642428386896739 1101 2 0.6708322326349361 1101 3 0.38934805478935886 1101 4 0.30135116532879636 1101 5 0.2571522731378266 1101 6 -1.2817701773632066 1101 7 0.5269876999178678 1101 8 0.6349450943596509 1101 9 0.763055891634906 1101 10-11 0.06601418199608133 1101 12-13 0.8945209101839708 1101 14-15 0.7215875520585229 1101 16-17 0.08140140301406973 1101 18-19 -0.0876109251016004 1101 20-21 -0.027503755191041535 1101 22-23 0.7466967265091569 1101 24-25 -2.0235903352822646 1101 26-27 -0.5053194414307569 1101 28-29 0.48035161490351896 1101 30-31 1.0443769913424035 1101 32-33 0.007423110315087911 1101 34-35 0.7404934826924787 1101 36-37 0.3618767498906337 1101 38-39 0.2069792616820365 1101 40-41 1.2505991645710495 1101 42-43 1.2461070307234294 1101 44-45 0.46578291524375004 1101 46-47 0.6916864526806776 1101 48-49 -1.905989958818033 1101 50-51 -1.6635375687886125 1101 52-53 -2.186936371851594 1101 54-55 -0.987576404694714 1101 56-57 -1.1897860775428626 1101 58-59 -0.82456580222577 1101 60-61 -1.7249098617353908 1101 62-63 -0.8087844689774855 1101 64-65 -1.3790341257289924 1101 66-67 -1.5653459494923325 1101 68-69 -0.9350972995967979 1101 70-71 -1.3709325959399514 1101 72-73 -2.094014036520907 1101 74-75 -0.7299812345779486 1101 76 -0.5838130019031809 1102 1 -0.7365341946250439 1102 2 0.22859791288805553 1102 3 -0.041533203353203874 1102 4 0.37137722153575936 1102 5 0.05628683483520547 1102 6 0.4471754768337064 1102 7 0.3179099035285162 1102 8 0.3924362538608648 1102 9 0.6438022158485666 1102 10-11 0.7640064581918793 1102 12-13 1.0192184720674646 1102 14-15 -0.02911038205649419 1102 16-17 0.3914237369057503 1102 18-19 -0.1531469385019406 1102 20-21 0.8454606036401557 1102 22-23 0.9271475919474668 1102 24-25 -4.83455488022922 1102 26-27 -1.209651285837971 1102 28-29 0.16768876361001617 1102 30-31 2.019786446023218 1102 32-33 0.6737757066299963 1102 34-35 1.0418614335579122 1102 36-37 -2.191352358614857 1102 38-39 0.0531429504964791 1102 40-41 1.1064874020547606 1102 42-43 0.632902117267264 1102 44-45 1.1487928724371272 1102 46-47 1.576457530290945 1102 48-49 -2.4976287081200965 1102 50-51 -0.21804622317163336 1102 52-53 0.2700243577221997 1102 54-55 0.8211547510841797 1102 56-57 1.0358792863134525 1102 58-59 0.07098835746155885 1102 60-61 -5.934499476475761 1102 62-63 -2.4786500003379928 1102 64-65 0.6148705829998349 1102 66-67 1.557374132398607 1102 68-69 -0.6848762871270466 1102 70-71 -0.7000550601126676 1102 72-73 -4.752573035218095 1102 74-75 -1.3822355617694555 1102 76 -0.17033356769510277 1103 1 0.6054610264024376 1103 2 0.48591571456069715 1103 3 0.16082641432899436 1103 4 0.5262457997914325 1103 5 0.29463808931549096 1103 6 0.4246247002506678 1103 7 1.1475096645798857 1103 8 0.8223944378513082 1103 9 0.707272944641872 1103 10-11 0.3326295167342863 1103 12-13 -0.490488815865632 1103 14-15 -0.2490924609095444 1103 16-17 0.07802767955808321 1103 18-19 0.44900359913247456 1103 20-21 -0.4174575564554246 1103 22-23 0.5900060148865336 1103 24-25 -2.825594306752521 1103 26-27 -0.5099798401987812 1103 28-29 -0.22060275371375582 1103 30-31 1.2728479752944253 1103 32-33 1.5373509754471968 1103 34-35 0.5817359855292423 1103 36-37 -0.3272543896781741 1103 38-39 -0.07200639239479756 1103 40-41 -0.1057288464518038 1103 42-43 -0.24456204043882934 1103 44-45 1.2062898855792952 1103 46-47 1.3981421181045661 1103 48-49 -2.134501467976726 1103 50-51 -0.23977561385263613 1103 52-53 0.6668284198488479 1103 54-55 -0.0336839585932438 1103 56-57 0.7507837068630323 1103 58-59 0.03716219258700448 1103 60-61 -4.320924207658557 1103 62-63 -1.2977210875542369 1103 64-65 0.10471526639290119 1103 66-67 -0.41902969077941066 1103 68-69 0.5137497582731925 1103 70-71 0.7603690736985627 1103 72-73 -4.730918315982731 1103 74-75 -1.9908825151744765 1103 76 -2.757848501984231 1104 1 0.21535281897082825 1104 2 0.4916663610830838 1104 3 0.4432886438104191 1104 4 0.0037025848737783917 1104 5 -0.311153390199955 1104 6 0.0610409245599115 1104 7 0.7039272499889009 1104 8 0.7023284245125296 1104 9 1.0904735707477684 1104 10-11 -0.43023287607910277 1104 12-13 0.5672728555648803 1104 14-15 0.7015506587311293 1104 16-17 0.38052359625877585 1104 18-19 0.788308757700598 1104 20-21 0.26110171943948757 1104 22-23 0.6561384498940157 1104 24-25 0.5436975810928573 1104 26-27 1.103229633055605 1104 28-29 1.4609084073043448 1104 30-31 1.1168914624320294 1104 32-33 0.5332166349000289 1104 34-35 0.3270231775804149 1104 36-37 1.5679069006444024 1104 38-39 1.3907249945658648 1104 40-41 2.116479197647827 1104 42-43 0.5256587979986307 1104 44-45 1.0731658213353867 1104 46-47 1.0122873474498775 1104 48-49 0.7669634156492435 1104 50-51 0.8158848598100832 1104 52-53 0.756780100807056 1104 54-55 0.11519483547236575 1104 56-57 -0.11203445020787939 1104 58-59 -0.9211881544934286 1104 60-61 0.5997832239461829 1104 62-63 0.2671012889259572 1104 64-65 0.7961879763425443 1104 66-67 0.05274450276897369 1104 68-69 1.8360156491129942 1104 70-71 1.271053706882178 1104 72-73 0.8465537814720108 1104 74-75 0.028717321493552106 1104 76 -0.4073823253134812 1105 1 0.9809468406270767 1105 2 0.6920628838831462 1105 3 0.46387732765973766 1105 4 0.25346111265579196 1105 5 0.16706327392489584 1105 6 0.40606659686940105 1105 7 1.1723833528814822 1105 8 -0.023003282060599872 1105 9 1.0881351029525348 1105 10-11 0.8128406839839357 1105 12-13 0.4092229343610825 1105 14-15 0.5886941694829915 1105 16-17 0.5524009792082936 1105 18-19 0.8774755514579056 1105 20-21 0.929268055670498 1105 22-23 0.7076864139019463 1105 24-25 0.899297194737315 1105 26-27 0.8409399680665359 1105 28-29 1.6140017935073843 1105 30-31 1.9665178159473555 1105 32-33 1.5430026142605158 1105 34-35 0.027470536636890586 1105 36-37 0.8470233665078553 1105 38-39 0.2092395591533318 1105 40-41 0.9037040464099633 1105 42-43 1.0120855175350059 1105 44-45 1.426096531517885 1105 46-47 1.5180572625533273 1105 48-49 0.3238237684528613 1105 50-51 1.4333928665204638 1105 52-53 1.166164473741837 1105 54-55 1.2245182048994394 1105 56-57 1.0099979833237143 1105 58-59 0.6222768447440217 1105 60-61 1.0760426921989321 1105 62-63 0.277903993414796 1105 64-65 1.1571508150391026 1105 66-67 1.2153951051786152 1105 68-69 0.9051783759697827 1105 70-71 1.8987289648761774 1105 72-73 1.492936781827865 1105 74-75 0.31647595094807457 1105 76 1.04023091244769 1106 1 -0.6976366522228261 1106 2 0.3773085394024207 1106 3 0.32143841312309007 1106 4 0.48603627311069886 1106 5 0.2761726084592979 1106 6 0.34571303308154455 1106 7 -0.9580073688840827 1106 8 0.5192967592260445 1106 9 -0.7057131874051592 1106 10-11 0.6996026852600679 1106 12-13 1.336802411146735 1106 14-15 -0.11452055858817545 1106 16-17 0.3675400401248581 1106 18-19 0.8604823449871617 1106 20-21 0.4522665918713713 1106 22-23 0.6938272388841185 1106 24-25 -0.1360216507380443 1106 26-27 0.9625103617909616 1106 28-29 0.8459295043507566 1106 30-31 1.0620588586227342 1106 32-33 0.26937539510350916 1106 34-35 1.4878635104011124 1106 36-37 1.5048227884948702 1106 38-39 0.4488897486965513 1106 40-41 1.1846502611755696 1106 42-43 0.9854462501852268 1106 44-45 1.4264624812983229 1106 46-47 1.3621532727623915 1106 48-49 1.768856234766723 1106 50-51 0.4165591766206802 1106 52-53 0.861318923315828 1106 54-55 0.13482297538324417 1106 56-57 0.9843908820213159 1106 58-59 1.3078133834220935 1106 60-61 1.2948618942407464 1106 62-63 1.0142324849510373 1106 64-65 -0.44163685902162797 1106 66-67 0.5854547831428079 1106 68-69 2.3766718264070477 1106 70-71 1.1652530049617482 1106 72-73 0.9926458986690605 1106 74-75 0.18156246522950426 1106 76 0.42186961679779955 1107 1 0.8108304777396285 1107 2 0.1584412177313581 1107 3 0.3897346029146007 1107 4 0.4703800705386101 1107 5 0.21654324509161427 1107 6 0.4660501207083527 1107 7 0.8325110431296494 1107 8 0.15596901739362323 1107 9 -0.3341180120716629 1107 10-11 0.6979452046306278 1107 12-13 0.2407284435774386 1107 14-15 0.3832827803366641 1107 16-17 0.7931331386151541 1107 18-19 0.8650154546604583 1107 20-21 1.1052540509336026 1107 22-23 -0.005010527710659574 1107 24-25 0.6787998206254784 1107 26-27 0.46093275974607195 1107 28-29 1.076698735119983 1107 30-31 1.3171511183878835 1107 32-33 1.6476004929547798 1107 34-35 0.6465622882587709 1107 36-37 1.7495308322683343 1107 38-39 0.8556714405249721 1107 40-41 0.6640016470690071 1107 42-43 1.378628612993758 1107 44-45 1.1219054080496562 1107 46-47 1.425816504649923 1107 48-49 1.328225992734609 1107 50-51 1.4518255717001551 1107 52-53 0.8305656682635103 1107 54-55 1.145406887836316 1107 56-57 1.4041129045470697 1107 58-59 1.2243003232735248 1107 60-61 1.6776800107037246 1107 62-63 1.1418984327104411 1107 64-65 1.2017651698944292 1107 66-67 0.6788128788373484 1107 68-69 0.9901593254085697 1107 70-71 -0.3093855444431526 1107 72-73 0.6585736884286248 1107 74-75 -0.580419322453217 1107 76 0.8613615890000546 1108 1 0.05993499240430822 1108 2 0.5302538219091062 1108 3 0.09353287932698606 1108 4 0.4244354665027146 1108 5 0.055480011414275054 1108 6 -0.17144677612464676 1108 7 0.1584854375687783 1108 8 -0.988691762121924 1108 9 -0.20493760280826478 1108 10-11 0.2911022780781565 1108 12-13 1.3053718453577687 1108 14-15 0.6315627391975411 1108 16-17 -0.3507385498623421 1108 18-19 0.30242014310248777 1108 20-21 1.0396265019035624 1108 22-23 0.004458564745981164 1108 24-25 1.2201074065388724 1108 26-27 0.0910441191105491 1108 28-29 -0.7733651014583742 1108 30-31 0.3244391277505869 1108 32-33 0.7143185835317922 1108 34-35 0.44308319473818614 1108 36-37 1.211060427615358 1108 38-39 1.5868662484832612 1108 40-41 1.1476257914815378 1108 42-43 -0.11317902611678221 1108 44-45 1.0232069649528768 1108 46-47 1.1304570692489904 1108 48-49 1.1440840627420528 1108 50-51 0.7380971608763147 1108 52-53 0.3168585362991152 1108 54-55 -0.5608473236046194 1108 56-57 0.2909891679793475 1108 58-59 0.35311721868792745 1108 60-61 1.1483151846584008 1108 62-63 1.1885257334871255 1108 64-65 0.8831585805409503 1108 66-67 0.9116656645947003 1108 68-69 1.176571000409453 1108 70-71 -0.7465242471420197 1108 72-73 0.060868258772991624 1108 74-75 -0.8860103427745329 1108 76 0.29587589903303524 1109 1 -0.7761222520702447 1109 2 0.5634996513612194 1109 3 0.4449936296022017 1109 4 0.3906138050580594 1109 5 0.06320293460844795 1109 6 0.03631001953967683 1109 7 -0.20588072383354472 1109 8 0.6530560573378068 1109 9 0.7378687313436529 1109 10-11 0.5929652639363923 1109 12-13 0.6459002558921085 1109 14-15 0.8842683027507618 1109 16-17 0.9908568434817155 1109 18-19 0.7242340138790198 1109 20-21 1.139668842491247 1109 22-23 0.37813965543952577 1109 24-25 0.0315732376846114 1109 26-27 0.07109323419226143 1109 28-29 -1.0714174344247525 1109 30-31 0.4333729917526199 1109 32-33 0.852772305269454 1109 34-35 0.5471902317438477 1109 36-37 1.4699477169709354 1109 38-39 1.4092559512523337 1109 40-41 0.422653312863531 1109 42-43 1.2117003032083105 1109 44-45 1.156512071227752 1109 46-47 1.1406865552342609 1109 48-49 1.0844007703379575 1109 50-51 0.3608086521118139 1109 52-53 -0.25971865059224086 1109 54-55 -0.13726361626275718 1109 56-57 0.42166915781761105 1109 58-59 0.3776493551939879 1109 60-61 1.2707726323677733 1109 62-63 0.25129142067486043 1109 64-65 0.7468811650104215 1109 66-67 0.13029552317713922 1109 68-69 -0.1470550475200909 1109 70-71 0.9752472830468193 1109 72-73 0.5906336919172048 1109 74-75 0.43933473301511583 1109 76 1.2088236507478314 1110 1 -0.9120847451755942 1110 2 -0.588844529554386 1110 3 -0.014631301451302647 1110 4 -0.19443859428005794 1110 5 0.39337392192229004 1110 6 0.05590921056744236 1110 7 0.9221391327577422 1110 8 0.8273711887957944 1110 9 1.1320404540868054 1110 10-11 -0.6015465948611762 1110 12-13 0.706655909504903 1110 14-15 1.0557244527783425 1110 16-17 0.6136459591279788 1110 18-19 0.039420629065631374 1110 20-21 0.9667694124489614 1110 22-23 -1.1818989545990846 1110 24-25 -0.9672500754244169 1110 26-27 1.0244578232711348 1110 28-29 0.3008844593057134 1110 30-31 -0.2984067471699774 1110 32-33 0.7396540850083682 1110 34-35 1.053873445569927 1110 36-37 1.347186179923682 1110 38-39 0.8794067142602415 1110 40-41 -0.9393709563035983 1110 42-43 -0.15913992477477734 1110 44-45 0.9944510305952861 1110 46-47 0.5903464191798378 1110 48-49 1.538031952540571 1110 50-51 -0.22667985680526925 1110 52-53 0.35961394731179297 1110 54-55 -0.36296687053744847 1110 56-57 0.6554989059330723 1110 58-59 -0.877397528424332 1110 60-61 0.06437439739811168 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-1.3432560769413016 2116 56-57 -0.22396973468206482 2116 58-59 -2.368015534965899 2116 60-61 -2.3806247418685444 2116 62-63 0.15075846864054654 2116 64-65 -2.386603821340813 2116 66-67 -1.1542618666577766 2116 68-69 -1.6574671104344247 2116 70-71 0.3744023590899772 2116 72-73 -0.34768685923319254 2116 74-75 -1.136968399702937 2116 76 -1.749450104614187 2117 1 0.3967307817607377 2117 2 0.5413930906161184 2117 3 0.3520474578449111 2117 4 -0.1085506929559159 2117 5 0.10569026511112156 2117 6 0.07211333415411048 2117 7 0.64307147449545 2117 8 -0.08432238397160319 2117 9 -2.129190575600603 2117 10-11 -0.7994878246815986 2117 12-13 -1.8414576402864995 2117 14-15 -0.17377854014747385 2117 16-17 -1.4338931907601733 2117 18-19 -2.03933262082856 2117 20-21 -0.9854647008589765 2117 22-23 -0.19817865712040117 2117 24-25 -2.4047916438384647 2117 26-27 -2.6410146962282255 2117 28-29 -2.323533582897568 2117 30-31 -0.7080596887021215 2117 32-33 -2.233243309029959 2117 34-35 0.45218954690616187 2117 36-37 -1.4622944416374821 2117 38-39 0.10253084510249266 2117 40-41 -0.37774389299868005 2117 42-43 0.009673183635648286 2117 44-45 -0.8672244032278087 2117 46-47 -1.296678189321284 2117 48-49 -1.4276249795593188 2117 50-51 -0.6644481620232341 2117 52-53 0.2498279868546902 2117 54-55 -1.3017479334795752 2117 56-57 0.03003787443176975 2117 58-59 -1.2692894203162197 2117 60-61 -1.3788507069008205 2117 62-63 -0.5744584765994887 2117 64-65 -1.5099740603011895 2117 66-67 -0.9774568395129108 2117 68-69 -0.24200094534231198 2117 70-71 -0.7276153385118675 2117 72-73 0.6834976831512805 2117 74-75 0.30647622048259393 2117 76 0.3800330741144933 2118 1 -1.0518559031949302 2118 2 -0.28562890517088135 2118 3 -1.5546786480679913 2118 4 -0.08327268384407205 2118 5 0.20004190377275677 2118 6 -0.2766350124877306 2118 7 0.5089412633554957 2118 8 0.05558845715905392 2118 9 -2.0638890520616826 2118 10-11 -1.3320161154766765 2118 12-13 -1.5535038853264211 2118 14-15 0.1043040542776481 2118 16-17 -4.613239685640885 2118 18-19 -1.4162247973827178 2118 20-21 -1.1667217429400054 2118 22-23 0.005564055856623895 2118 24-25 0.7271340897626715 2118 26-27 -3.5506556113313508 2118 28-29 -1.5464062620726011 2118 30-31 -2.455916446650477 2118 32-33 -1.5882376753651357 2118 34-35 -1.1383899857446025 2118 36-37 -4.494136894976034 2118 38-39 -1.0235162817284476 2118 40-41 -0.8627779399295576 2118 42-43 0.36505945708884013 2118 44-45 -2.572804180820512 2118 46-47 -0.6699942134235712 2118 48-49 -0.7304956991987623 2118 50-51 -1.6682692564238621 2118 52-53 -2.7977642337890174 2118 54-55 -2.2486445189888116 2118 56-57 -0.2924526953031972 2118 58-59 -1.6415521940388444 2118 60-61 -2.144049354821263 2118 62-63 -2.372185415315016 2118 64-65 -0.8643805659593369 2118 66-67 -0.33411681109964064 2118 68-69 -2.254101077610958 2118 70-71 -1.204076417236216 2118 72-73 0.9360083381260509 2118 74-75 0.019477739122677917 2118 76 0.13910817331598224 2119 1 0.659762101671248 2119 2 -1.5167724574823147 2119 3 -0.1878294996494958 2119 4 -1.1554746303160925 2119 5 -0.4298242762759088 2119 6 -0.7130097083514784 2119 7 -0.8922752816566728 2119 8 -0.9149711535465457 2119 9 -0.9913829693366196 2119 10-11 -0.9989565047710869 2119 12-13 -0.7539296760806806 2119 14-15 -0.8693881598342728 2119 16-17 -0.8905207075386912 2119 18-19 -0.2531469385019349 2119 20-21 -1.4077801371005947 2119 22-23 -0.18257463027476462 2119 24-25 0.2515562308818886 2119 26-27 -0.47678091546760015 2119 28-29 -0.8415704956492434 2119 30-31 0.01747163120840156 2119 32-33 -0.3845576267033408 2119 34-35 -0.1511941219976407 2119 36-37 -0.5945930993555919 2119 38-39 -1.6667644569109257 2119 40-41 -1.515271857204496 2119 42-43 -1.2518201049549518 2119 44-45 -0.757688609044358 2119 46-47 -1.5763202474868265 2119 48-49 -1.4948509303423165 2119 50-51 -0.36249066761607907 2119 52-53 -0.2609941607963222 2119 54-55 -0.6121785822491574 2119 56-57 -2.834028121093951 2119 58-59 -1.2734560869828826 2119 60-61 -1.1053328098090915 2119 62-63 -1.5837425929305837 2119 64-65 -1.126333120703869 2119 66-67 -0.40142216389769203 2119 68-69 -0.14969110194185475 2119 70-71 -0.45051802307563094 2119 72-73 0.13400103885597758 2119 74-75 -0.8086244506583462 2119 76 -0.11107430843584254 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 5.0 22 12.0 23 21.0 24 65.0 25 126.0 26 242.0 27 460.0 28 752.0 29 1293.0 30 1967.0 31 3000.0 32 4374.0 33 6287.0 34 8827.0 35 11400.0 36 15387.0 37 21283.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.88249317898863 22.088156605123043 10.528197928531695 31.501152287356625 2 18.74652327090673 18.06177319806098 42.03597255701836 21.155730974013935 3 16.40883685200392 26.235066620751766 34.80305157479272 22.55304495245159 4 11.8976980742232 21.53452888665201 34.744774972848404 31.82299806627639 5 11.092421392810786 38.960557336229506 35.710312309607694 14.236708961352019 6 23.228523747715293 37.33146141824057 16.657836878493285 22.782177955550846 7 23.433816322746416 35.83481232285238 18.87764562528145 21.85372572911976 8 20.40740642631983 34.90503562819528 23.31593865063177 21.37161929485312 9 19.6166988953935 20.07496490159201 17.875023178193956 42.433313024820535 10-11 20.08953405207809 24.654313793012108 30.06344202802575 25.192710126884055 12-13 23.771555720378267 19.549813249980133 27.20921300098011 29.469418028661494 14-15 23.539773780826998 23.89406903128394 20.752430399194726 31.81372678869434 16-17 24.077507880585944 27.017165108209053 20.91666445921962 27.988662551985378 18-19 21.892135307673968 27.375433763344013 22.059680538263887 28.67275039071813 20-21 21.637175174167574 24.654313793012108 21.983523615268467 31.72498741755185 22-23 23.64771794124659 29.57471325262907 21.919286906307118 24.85828189981722 24-25 23.579507827607216 31.189902254244924 22.262324176843 22.968265741304865 26-27 21.458371963656592 28.71910677862838 29.432332918333287 20.390188339381737 28-29 21.878890625413895 26.407909724245716 29.65087017562449 22.062329474715902 30-31 22.453047601388043 24.13181107785224 27.448279515774416 25.966861804985296 32-33 18.781621678895924 26.1681809753384 26.35956663399645 28.690630711769227 34-35 17.811448703345604 26.33903737649334 28.021112023522555 27.8284018966385 36-37 17.939259887155306 26.607242192259804 31.448173558316334 24.00532436226855 38-39 21.00937723504013 23.6497046435856 28.564806230298533 26.776111891075733 40-41 19.578951550952294 24.971523933140844 31.036263940028082 24.413260575878784 42-43 24.77351593335276 23.443749834441473 31.656115069799473 20.126619162406296 44-45 19.457100474159624 25.121851076792666 29.164790336679825 26.256258112367885 46-47 24.308627586024212 21.57293846520622 28.14627427088024 25.97215967788933 48-49 21.86498370904082 21.037191067786285 29.45617334640142 27.641651876771476 50-51 17.475033773939764 21.50207941511483 33.238854599878145 27.784032211067256 52-53 19.31869354454187 21.489496966967764 36.732139545972295 22.45966994251808 54-55 17.5723821885513 21.83385870572965 33.45540515483034 27.138353950888717 56-57 22.48351037058621 21.739821461683135 30.502503244947153 25.274164922783505 58-59 17.93594871659029 28.66215464491007 31.878625731768693 21.52327090673095 60-61 19.446504728351567 35.02556223676194 25.027151598633147 20.500781436253344 62-63 15.953219782257424 40.31548833143493 22.827872109348096 20.90341977695955 64-65 15.823421896108714 41.176392678339646 21.8067071070965 21.19347831845514 66-67 15.940637334110356 40.57707080607136 21.47029217769066 22.011999682127627 68-69 15.668459113665865 38.5950041058515 21.823925194034597 23.91261158644804 70-71 16.146592143254484 36.26394002807873 22.5543694206776 25.035098407989192 72-73 16.45254430346216 33.723609970596804 23.075547667611453 26.748298058329578 74-75 16.771078911816907 33.27130407141532 23.294084924902652 26.663532091865115 76 16.790283701094012 33.40441312812906 22.839130089269158 26.966173081507776 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.5 14 2.0 15 2.5 16 4.0 17 6.0 18 5.5 19 11.5 20 18.5 21 19.0 22 28.0 23 49.5 24 76.5 25 91.0 26 137.0 27 226.5 28 308.0 29 346.0 30 425.5 31 628.0 32 861.5 33 972.0 34 1099.0 35 1364.0 36 1696.0 37 1890.0 38 2128.5 39 2935.0 40 4655.5 41 6688.0 42 7568.0 43 7497.5 44 7011.5 45 6130.5 46 5665.0 47 5305.0 48 4665.5 49 4034.5 50 3683.0 51 3601.0 52 3114.0 53 2498.5 54 2288.0 55 2089.0 56 1693.0 57 1381.0 58 1266.0 59 1033.5 60 700.5 61 519.0 62 438.0 63 369.5 64 235.0 65 140.0 66 76.0 67 41.0 68 29.5 69 12.5 70 7.0 71 7.0 72 4.0 73 1.0 74 0.5 75 0.0 76 0.0 77 0.5 78 0.5 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 75502.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.07554766761145 #Duplication Level Percentage of deduplicated Percentage of total 1 67.61254063584771 32.50509920267013 2 16.63452531819935 15.994278297263648 3 6.766213014491157 9.758681889221478 4 3.2673976527632376 6.283277264178433 5 1.84307675354014 4.430346215994279 6 1.099234117582236 3.1707769330613758 7 0.6584384814590336 2.2158353421101427 8 0.5344647087993828 2.0555746867632645 9 0.3526365088985619 1.5257873963603614 >10 1.1681084357264864 8.089851924452333 >50 0.02754972725770015 0.9469947815951896 >100 0.02754972725770015 2.5986066594262405 >500 0.0 0.0 >1k 0.008264918177310045 10.424889406903128 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTCTT 3712 4.916426054938942 RNA PCR Primer, Index 27 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGT 2936 3.8886387115573093 No Hit CTTTACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTC 1223 1.6198246404068768 No Hit CTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTC 472 0.6251490026754258 No Hit TCTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCG 300 0.3973404678021774 No Hit TATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTCT 270 0.35760642102195966 No Hit CTCTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCC 174 0.2304574713252629 No Hit TCTTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCC 142 0.18807448809303065 No Hit CTTATAACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCG 130 0.17218086938094354 No Hit CTTAATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCG 126 0.1668829964769145 No Hit CTTATACACATCTCCGAGCCCACGAGACTTGACCCTATATCGTATGCCGT 125 0.16555852825090725 No Hit TCTTAATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCC 115 0.15231384599083467 No Hit ATACACATCTCCGAGCCCACGAGACTTGACCCTAACTCGTATGCCGTCTT 108 0.14304256840878388 No Hit CTTATACACATCTCCGAGCCCACGAGACTTGACCCTACCTCGTATGCCGT 90 0.11920214034065323 No Hit CTTAACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTC 88 0.11655320388863871 No Hit ATACACATCTCCGAGCCCACGAGACTTGACCCTATATCGTATGCCGTCTT 86 0.1139042674366242 No Hit CTTATACACATCTCCGAGCCCACGAGACTTGACCCTAACTCGTATGCCGT 78 0.10330852162856613 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0026489364520145163 0.0 2 0.0 0.0 0.0 0.0026489364520145163 0.0 3 0.0 0.0 0.0 0.0026489364520145163 0.0 4 0.0 0.0 0.0 0.0026489364520145163 0.0 5 0.0 0.0 0.0 0.003973404678021774 0.0 6 0.0 0.0 0.0 0.003973404678021774 0.0 7 0.0 0.0 0.0 0.003973404678021774 0.0 8 0.0 0.0 0.0 0.003973404678021774 0.0 9 0.0 0.0 0.0 0.01324468226007258 0.0 10 0.0 0.0 0.0 0.01324468226007258 0.0 11 0.0 0.0 0.0 0.01324468226007258 0.0 12 0.0 0.0 0.0 0.01324468226007258 0.0 13 0.0 0.0 0.0 0.015893618712087096 0.0 14 0.0 0.0 0.0 0.018542555164101613 0.0 15 0.0 0.0 0.0 0.01986702339010887 0.0 16 0.0 0.0 0.0 0.01986702339010887 0.0 17 0.0 0.0 0.0 0.02119149161611613 0.0 18 0.0 0.0 0.0 0.023840428068130647 0.0 19 0.0 0.0 0.0 0.029138300972159678 0.0 20 0.0 0.0 0.0 0.03178723742417419 0.0 21 0.0 0.0 0.0 0.03178723742417419 0.0 22 0.0 0.0 0.0 0.03443617387618871 0.0 23 0.0 0.0 0.0 0.03840957855421048 0.0 24 0.0 0.0 0.0 0.04238298323223226 0.0 25 0.0 0.0 0.0 0.04370745145823952 0.0 26 0.0 0.0 0.0 0.05297872904029032 0.0 27 0.0 0.0 0.0 0.07946809356043548 0.0 28 0.0 0.0 0.0 0.0980106487245371 0.0 29 0.0 0.0 0.0 0.12450001324468225 0.003973404678021774 30 0.0 0.0 0.0 0.16290959179889275 0.003973404678021774 31 0.0 0.0 0.0 0.205292575031125 0.003973404678021774 32 0.0 0.0 0.0 0.25694683584540806 0.003973404678021774 33 0.0 0.0 0.0 0.3099255648856984 0.003973404678021774 34 0.0 0.0 0.0 0.3761489761860613 0.003973404678021774 35 0.0 0.0 0.0 0.4847553707186565 0.003973404678021774 36 0.0 0.0 0.0 0.6225000662234113 0.003973404678021774 37 0.0 0.0 0.0 0.897989457232921 0.003973404678021774 38 0.0 0.0 0.0 1.2039416174405977 0.003973404678021774 39 0.0 0.0 0.0 1.5615480384625573 0.003973404678021774 40 0.0 0.0 0.0 2.0502768138592353 0.003973404678021774 41 0.0 0.0 0.0 2.6356917697544437 0.003973404678021774 42 0.0 0.0 0.0 3.3111705650181453 0.003973404678021774 43 0.0 0.0 0.0 3.997245106089905 0.003973404678021774 44 0.0 0.0 0.0 4.643585600381447 0.003973404678021774 45 0.0 0.0 0.0 5.402505893883606 0.003973404678021774 46 0.0 0.0 0.0 6.103149585441446 0.003973404678021774 47 0.0 0.0 0.0 6.672670922624566 0.003973404678021774 48 0.0 0.0 0.0 7.268681624327832 0.003973404678021774 49 0.0 0.0 0.0 7.803766787634765 0.003973404678021774 50 0.0 0.0 0.0 8.41037323514609 0.003973404678021774 51 0.0 0.0 0.0 8.978570104103202 0.003973404678021774 52 0.0 0.0 0.0 9.599745702100607 0.003973404678021774 53 0.0 0.0 0.0 10.307011734788482 0.003973404678021774 54 0.0 0.0 0.0 11.026197981510423 0.003973404678021774 55 0.0 0.0 0.0 11.63147996079574 0.003973404678021774 56 0.0 0.0 0.0 12.26457577282721 0.003973404678021774 57 0.0 0.0 0.0 12.75727795290191 0.003973404678021774 58 0.0 0.0 0.0 13.381102487351328 0.003973404678021774 59 0.0 0.0 0.0 13.851288707583905 0.003973404678021774 60 0.0 0.0 0.0 14.439352599931128 0.003973404678021774 61 0.0 0.0 0.0 14.9267569071018 0.003973404678021774 62 0.0 0.0 0.0 15.494953776058912 0.003973404678021774 63 0.0 0.0 0.0 15.996927233715663 0.003973404678021774 64 0.0 0.0 0.0 16.58234218961087 0.003973404678021774 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACATATG 15 0.00221684 70.0 1 CCTTGAA 15 0.00221684 70.0 56 AGTTTGG 20 0.0069270306 52.500004 8 AGCCTTG 20 0.0069270306 52.500004 22 GAACAGT 20 0.0069270306 52.500004 13 TATAACA 20 0.0069270306 52.500004 3 CTTTACA 210 0.0 51.666668 1 TTTACAC 215 0.0 48.83721 2 TTACACA 225 0.0 46.666668 3 ACATAGA 30 5.844378E-4 46.666664 1 AAAAGTA 35 0.0012483324 40.000004 31 CCTCTAG 35 0.0012483324 40.000004 1 GTGAGTA 45 9.120466E-5 38.88889 61 CTATACA 75 3.4677214E-8 37.333336 1 CTTGGGC 50 1.6954217E-4 35.000004 47 GTTGTTG 50 1.6954217E-4 35.000004 21 TATTAAG 50 1.6954217E-4 35.000004 15 TCTTTAT 40 0.0024051927 35.000004 1 CTTTATA 40 0.0024051927 35.000004 2 TTTGATA 50 1.6954217E-4 35.000004 37 >>END_MODULE