FastQCFastQC Report
Wed 25 May 2016
SRR1780465_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780465_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences542429
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACAT353.221515E-431.418621
CGGTGGT1000.030.77037643
TGTCCGT300.005727843429.34841534
TCGAGAT407.070542E-427.4710169
CGACTAA450.001393790524.45475437
CGTGCGA952.66391E-823.16980210
CAGTTAC500.002583363821.9930341
CTTATAA602.8760993E-421.9930341
GGTGGTC1407.2759576E-1221.9788444
TTCGAGA500.002594456321.9768148
CGGACTA1004.663889E-821.9768149
CGAGAAT1158.609277E-921.0718114
TCGGTGG2100.020.9380242
CGAAACT956.872815E-720.83550625
AGTTCGA654.9625005E-420.286296
TCGTTTG550.004469551620.00843440
TGCGAGT550.004469551620.00843436
CACAGTA550.004469551620.00843436
GATCTAG550.004488693619.9936681
CGCTATT550.0044934919.98997927
Produced by FastQC (version 0.11.5)