FastQCFastQC Report
Wed 25 May 2016
SRR1780465_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780465_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences542429
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGT302685.580085135566129No Hit
ATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTCTT249684.602998733474796Illumina PCR Primer Index 10 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTC70341.2967595759076305No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCG37660.6942844132596155No Hit
CTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTC36600.6747426852177889No Hit
TATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTCT21920.40410818743098176No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCC19350.3567287147257982No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCC12480.23007619430377063No Hit
CTTAATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCG8900.1640767731813749No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCC8590.1583617395087652No Hit
CTTATAACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCG8020.14785345178816028No Hit
TTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTC6050.11153533457835035No Hit
CTTAACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTC5700.10508287720604909No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGC207.854987E-443.9975143
CCGTCTT27800.042.41486744
CGTGCGA452.3481334E-839.108910
GCCGTCT30300.038.84268643
CTATACA5650.037.4474641
TTTACAC9400.036.7561572
CTTTACA9700.035.6720051
CTCGTTT250.002352305735.1980120
TTACACA9900.034.8997843
CTTATAA1050.031.4848461
CTTTATA2200.031.0096822
CAATACC300.005733529.3424934
CCGTGCG602.9417788E-729.3316739
TTGTATC300.00574384529.33167341
TAACGCT300.00574384529.33167337
TCTTTAT2500.028.2104231
TTATACA49300.027.9438172
CTTATAC49400.027.9284631
CGGTAAA804.307367E-927.49844426
TGCCGTC44500.026.4479442
Produced by FastQC (version 0.11.5)