FastQCFastQC Report
Wed 25 May 2016
SRR1780464_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780464_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14743
Sequences flagged as poor quality0
Sequence length76
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAACA150.002123608570.47261
CGCCGAC200.00681959252.494922
TTATACA1001.0921212E-831.496942
TCTGACG901.5686965E-731.10808811
TGCCGAC604.754478E-429.16383216
GCTGCCG604.754478E-429.16383217
CTGACGC853.6241272E-628.82072812
TATACAC1252.9922376E-927.997283
ACACATC1252.9922376E-927.997286
CTTATAC905.3592903E-627.4060121
ATCTGAC1154.2553438E-827.38864310
CTGCCGA700.00117055724.9975715
ACATCTG1051.870474E-523.3310668
GACGCTG750.001749758323.33106411
CATCTGA1102.6805672E-522.2705639
CGCTGCC800.00254658121.87287513
GCCGACG800.00254658121.87287517
CCGACGA800.00254658121.87287518
TACACAT1355.3918047E-620.7387245
TGACGCT850.003620258320.58623510
Produced by FastQC (version 0.11.5)