##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780464_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 14743 Sequences flagged as poor quality 0 Sequence length 76 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 18.921250763074003 12.0 12.0 23.0 12.0 32.0 2 18.199416672319067 12.0 11.0 23.0 11.0 32.0 3 18.953605100725767 21.0 11.0 23.0 11.0 32.0 4 18.483551515973684 11.0 11.0 23.0 11.0 32.0 5 19.506545479210473 21.0 11.0 27.0 11.0 33.0 6 21.475683375161093 21.0 11.0 31.0 11.0 36.0 7 22.284948789255917 23.0 11.0 34.0 11.0 36.0 8 22.138845553822154 22.0 11.0 33.0 11.0 36.0 9 22.19690700671505 21.0 12.0 33.0 12.0 36.0 10-11 20.751271790002036 21.0 11.0 31.0 11.0 35.0 12-13 20.513260530421217 21.0 11.0 28.0 11.0 34.0 14-15 20.388387709421423 21.0 11.0 29.5 11.0 34.0 16-17 19.83317506613308 16.5 11.0 27.5 11.0 34.0 18-19 20.277962422844737 21.0 11.0 28.0 11.0 34.0 20-21 20.31455606050329 21.0 11.0 29.0 11.0 34.0 22-23 19.682866445092586 16.0 11.0 27.5 11.0 34.0 24-25 19.814522146103236 16.0 11.0 27.0 10.5 34.0 26-27 19.32880010852608 11.5 11.0 26.0 10.5 34.0 28-29 18.90690497185105 11.0 11.0 26.0 11.0 34.0 30-31 18.736145967577833 11.0 11.0 26.0 11.0 34.0 32-33 19.15658278505053 11.0 11.0 26.0 11.0 34.0 34-35 19.486095095977753 16.0 11.0 26.5 11.0 34.0 36-37 19.22149494675439 11.0 11.0 26.0 11.0 34.0 38-39 18.482907142372653 11.0 11.0 26.0 11.0 34.0 40-41 18.920436817472698 11.0 11.0 26.0 11.0 34.0 42-43 19.09099233534559 11.0 11.0 26.0 11.0 34.0 44-45 18.81384385810215 11.0 11.0 26.0 11.0 34.0 46-47 19.015464966424744 11.0 11.0 26.0 11.0 34.0 48-49 19.472359763955776 16.0 11.0 26.5 11.0 34.0 50-51 19.4813131655701 16.0 11.0 26.0 11.0 34.0 52-53 19.357423862171878 15.0 11.0 26.0 11.0 34.0 54-55 19.4224377670759 20.0 11.0 26.0 11.0 34.0 56-57 18.742284473987656 15.0 11.0 26.0 11.0 33.5 58-59 18.996778131994844 16.0 11.0 26.0 11.0 33.5 60-61 19.066980940107168 15.0 11.0 26.0 11.0 33.0 62-63 18.62371973139795 15.0 11.0 25.5 11.0 33.0 64-65 19.028284609645254 15.0 11.0 26.0 11.0 33.0 66-67 19.45736959913179 19.0 11.0 26.0 11.0 33.0 68-69 18.745947229193515 15.0 11.0 25.0 11.0 33.0 70-71 18.90080037984128 15.0 11.0 25.0 11.0 33.0 72-73 18.590347961744556 11.0 11.0 25.0 11.0 33.0 74-75 18.865902462185446 15.0 11.0 25.0 11.0 33.0 76 18.634945397815912 19.0 11.0 25.0 11.0 32.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.02764536221437197 1101 2 -0.9527612222634332 1101 3 -4.0932135032163295 1101 4 -1.3238749089300725 1101 5 -2.45117265004507 1101 6 0.7240351371257887 1101 7 -1.0563076259889108 1101 8 -2.014954997679773 1101 9 0.19722504619956283 1101 10-11 1.3067202058646004 1101 12-13 0.1625422415646689 1101 14-15 -3.4758127434399633 1101 16-17 -1.0193122581141054 1101 18-19 -2.9815813896732415 1101 20-21 -0.961644205533112 1101 22-23 -0.5428445989007571 1101 24-25 -0.6341436666936495 1101 26-27 -3.3453458880240134 1101 28-29 -3.717838475797926 1101 30-31 -1.2267958062664803 1101 32-33 -2.267473056995197 1101 34-35 -1.3692193368551884 1101 36-37 -1.192499834652459 1101 38-39 -2.3172215749491443 1101 40-41 -0.6563562796092519 1101 42-43 -0.8393344368520275 1101 44-45 0.28369736598112993 1101 46-47 -1.8710562218491766 1101 48-49 -1.658435760231857 1101 50-51 -1.7139794685253094 1101 52-53 -2.218690764188839 1101 54-55 -1.3931013398920982 1101 56-57 -1.676474879711595 1101 58-59 -1.8611789670667953 1101 60-61 -2.8056951151455003 1101 62-63 -1.0855945185942062 1101 64-65 -1.7675125208330584 1101 66-67 -0.9611610697673534 1101 68-69 -1.4557867898922083 1101 70-71 -0.7902814757185794 1101 72-73 -1.532540683354945 1101 74-75 -0.32457904178776076 1101 76 -4.645861800238372 1102 1 2.01488259945161 1102 2 1.850542081039869 1102 3 1.5081378481350214 1102 4 -1.0168178518730144 1102 5 0.991770292897872 1102 6 -2.942631529540879 1102 7 -2.789791109472393 1102 8 1.424984942260167 1102 9 -0.9867088877343733 1102 10-11 -0.4605470614026643 1102 12-13 -0.5457910917686668 1102 14-15 -0.5674043350315543 1102 16-17 -0.18034829415014286 1102 18-19 -0.19029009838195066 1102 20-21 -0.42635892024782507 1102 22-23 0.24994819389203826 1102 24-25 -0.5080175405675256 1102 26-27 -0.2620125546906813 1102 28-29 -2.4783489863084363 1102 30-31 -4.016210220680893 1102 32-33 -1.853058642580784 1102 34-35 -0.769369487005342 1102 36-37 -1.1782355703881962 1102 38-39 -1.398302656030225 1102 40-41 -3.719419342672314 1102 42-43 -0.7623824849000762 1102 44-45 -0.5717831144993504 1102 46-47 1.5688837180907633 1102 48-49 0.789011687215595 1102 50-51 -0.801817306363148 1102 52-53 0.5200479745499003 1102 54-55 -2.3311644029551637 1102 56-57 -1.8457691740058912 1102 58-59 -1.9291219100097372 1102 60-61 -1.823337757788142 1102 62-63 -1.4669758999755906 1102 64-65 -1.6905605688811072 1102 66-67 -2.295620529226813 1102 68-69 -1.9726786817840996 1102 70-71 -1.3717379321750371 1102 72-73 -0.348606749421009 1102 74-75 -0.23974420695292764 1102 76 -4.6788948332714035 1103 1 -0.03562245105344175 1103 2 0.08286531336310077 1103 3 0.32631966631683795 1103 4 2.031161946106785 1103 5 0.7846995858271661 1103 6 1.4513078643985153 1103 7 -0.5145385842198671 1103 8 -0.05733829006306479 1103 9 -0.27206242308790607 1103 10-11 -1.9580218088774153 1103 12-13 -0.30336684934442104 1103 14-15 -0.05351544614266501 1103 16-17 -0.6197422335440805 1103 18-19 0.22511394202208734 1103 20-21 0.1317218878329811 1103 22-23 0.9166148605587026 1103 24-25 -2.7087751163250964 1103 26-27 -0.5385277062058336 1103 28-29 -0.49855100651045703 1103 30-31 -0.3419677964384711 1103 32-33 -2.311391975914116 1103 34-35 -0.7592684769043316 1103 36-37 -0.4901042572568812 1103 38-39 -2.523302656030225 1103 40-41 -0.7383587366117084 1103 42-43 1.6744862019686089 1103 44-45 -0.6235507912670251 1103 46-47 -0.5611667869597419 1103 48-49 -2.448362050158142 1103 50-51 -1.438180942726781 1103 52-53 -0.41555808605616207 1103 54-55 -1.3614674332581913 1103 56-57 -2.990971194207912 1103 58-59 -0.44932393021175443 1103 60-61 -0.9546508891012699 1103 62-63 -0.8217738797735663 1103 64-65 -1.6400555183760552 1103 66-67 1.4089249253186402 1103 68-69 -2.5976786817840996 1103 70-71 -0.32375813419524135 1103 72-73 -0.3132532140674762 1103 74-75 -0.8899967322054536 1103 76 -0.4566726110491821 1104 1 1.2490095835785944 1104 2 -1.3002515697537795 1104 3 -1.7418621518649786 1104 4 -2.639833724888888 1104 5 -0.15902335789578004 1104 6 -1.5854886723980215 1104 7 -4.793759363440646 1104 8 -2.0432690259938013 1104 9 -2.8240104750359585 1104 10-11 -1.797848648704253 1104 12-13 -0.2660291870067617 1104 14-15 -0.9642297318569497 1104 16-17 -2.1148721036739495 1104 18-19 0.09145593336408098 1104 20-21 -1.4462001900890975 1104 22-23 -0.17862323467939234 1104 24-25 -2.3056365881865695 1104 26-27 -2.952488745166871 1104 28-29 1.0295875216280734 1104 30-31 0.18021835074767978 1104 32-33 -0.9006776901998315 1104 34-35 -1.3804805981164492 1104 36-37 -1.6583943005469273 1104 38-39 -0.11258837031594027 1104 40-41 -1.4218002950532664 1104 42-43 -0.34769994521753844 1104 44-45 -0.5975767652930024 1104 46-47 -1.5184178692108254 1104 48-49 -0.06416291595900958 1104 50-51 -0.06372206826790716 1104 52-53 -0.06328535878343544 1104 54-55 0.3176451208543618 1104 56-57 -1.6652136184503377 1104 58-59 0.3823860264981995 1104 60-61 -0.372940932391316 1104 62-63 -1.284436217435907 1104 64-65 -0.2282589815795184 1104 66-67 -1.4920491006553824 1104 68-69 -1.5143453484507639 1104 70-71 1.1183414329043266 1104 72-73 -1.594638495452756 1104 74-75 -0.12863309584181692 1104 76 -0.1471488015253719 1105 1 1.253062268482342 1105 2 -1.1482758858632032 1105 3 -0.0610110880351904 1105 4 0.6007944412711943 1105 5 0.17734948911536463 1105 6 0.6602053498917471 1105 7 -0.6934554120728649 1105 8 0.6578454623547287 1105 9 -1.8194512045192432 1105 10-11 0.3538737423798395 1105 12-13 1.0774358585859467 1105 14-15 2.534250511304144 1105 16-17 -1.0943553863487239 1105 18-19 -0.8935997910533473 1105 20-21 -0.4978719226118926 1105 22-23 -0.6897681181647002 1105 24-25 -2.025748037021426 1105 26-27 -0.16449482419422878 1105 28-29 1.500965434495349 1105 30-31 -0.2759619937305402 1105 32-33 1.4838207900433318 1105 34-35 -0.9638139314497849 1105 36-37 0.570582396514876 1105 38-39 -0.3344728687961833 1105 40-41 1.6671054800227196 1105 42-43 -0.33300896244144695 1105 44-45 0.3043989185975775 1105 46-47 -0.9961281022402062 1105 48-49 -1.68143748536124 1105 50-51 -0.2752215616822973 1105 52-53 -0.7707321672940743 1105 54-55 -0.11219277174948772 1105 56-57 1.162800565946828 1105 58-59 0.9358308086663882 1105 60-61 -0.39396423532951275 1105 62-63 1.4313692536887181 1105 64-65 3.5154086982583728 1105 66-67 0.8772518111987182 1105 68-69 0.45019365864143523 1105 70-71 -0.3791256390308284 1105 72-73 0.5586036524702429 1105 74-75 2.440515840328395 1105 76 1.7135401549082658 1106 1 0.23710482167383162 1106 2 1.028319858817646 1106 3 0.3081378481350221 1106 4 2.622071037015875 1106 5 -0.6221185959910187 1106 6 0.32403513712579013 1106 7 1.6740977794164955 1106 8 2.3472071644823878 1106 9 0.4188466678211853 1106 10-11 -0.5091581725137786 1106 12-13 -0.7999577584353297 1106 14-15 0.508984553857335 1106 16-17 -0.07201496081680858 1106 18-19 -0.1180678761597278 1106 20-21 0.4805855241966164 1106 22-23 -1.3958851394412974 1106 24-25 -1.7996842072341899 1106 26-27 -0.10784588802401274 1106 28-29 1.1230399025804516 1106 30-31 0.254623112652439 1106 32-33 -0.9238919759141169 1106 34-35 1.5236860685502158 1106 36-37 0.5564866518340281 1106 38-39 -0.6983026560302257 1106 40-41 0.6514139906610197 1106 42-43 -0.2596047071222962 1106 44-45 0.03099466327842748 1106 46-47 0.616105940312984 1106 48-49 -0.7415438683399636 1106 50-51 0.9231826936368535 1106 52-53 0.43671464121656456 1106 54-55 0.5771689303781713 1106 56-57 0.6556197148829987 1106 58-59 -0.7402330211208472 1106 60-61 0.5794400199896366 1106 62-63 -0.8308647888644778 1106 64-65 -1.5002827911033272 1106 66-67 0.1793794707731884 1106 68-69 0.052321318215899026 1106 70-71 -1.6828490432861507 1106 72-73 -0.11666230497656471 1106 74-75 0.058866904158183075 1106 76 -0.0066726110491828194 1107 1 0.09127148834049947 1107 2 0.29498652548431537 1107 3 1.8206378481350214 1107 4 1.4137377036825427 1107 5 2.2070480706756506 1107 6 1.0573684704591209 1107 7 1.765764446083164 1107 8 0.8972071644823885 1107 9 2.964680001154516 1107 10-11 1.2512584941528857 1107 12-13 1.287542241564669 1107 14-15 1.727734553857335 1107 16-17 1.9029850391831928 1107 18-19 1.604848790506935 1107 20-21 0.8410021908632856 1107 22-23 0.48953152722536686 1107 24-25 0.929482459432478 1107 26-27 1.2692374453093223 1107 28-29 1.2647065692471173 1107 30-31 0.5775397793191033 1107 32-33 1.9073580240858838 1107 34-35 1.5361860685502151 1107 36-37 1.7002366518340253 1107 38-39 0.9350306773031107 1107 40-41 1.103497323994354 1107 42-43 -0.6200213737889619 1107 44-45 0.7684946632784282 1107 46-47 1.676522606979649 1107 48-49 2.1605394649933736 1107 50-51 1.9585993603035163 1107 52-53 1.1762979745498967 1107 54-55 0.2105022637115077 1107 56-57 -0.2277136184503341 1107 58-59 1.34518364554582 1107 60-61 0.08985668665630087 1107 62-63 0.4045518778021915 1107 64-65 2.4413838755633357 1107 66-67 1.829379470773187 1107 68-69 1.2252379848825683 1107 70-71 0.26715095671385214 1107 72-73 -0.060412304976566134 1107 74-75 1.777616904158183 1107 76 1.25166072228415 1108 1 0.05528663985564819 1108 2 -0.39440741390962586 1108 3 -2.3327712427740686 1108 4 -3.127928962984125 1108 5 0.034699585827166146 1108 6 1.746762409853062 1108 7 -1.9009022205835038 1108 8 -0.4891564718812482 1108 9 -1.6129715139969996 1108 10-11 -0.6057490816046851 1108 12-13 -1.7010941220716944 1108 14-15 -0.37169726432448513 1108 16-17 0.9938941300922828 1108 18-19 -1.0589769670688192 1108 20-21 -0.4137326576215621 1108 22-23 -0.5720215030776608 1108 24-25 1.0980430654930835 1108 26-27 0.06374502106689306 1108 28-29 -0.6349146428740937 1108 30-31 -1.7737859782566545 1108 32-33 -0.5273010668232097 1108 34-35 -0.7024502950861482 1108 36-37 -2.103740620893248 1108 38-39 -1.1255753833029516 1108 40-41 -1.1929041911571616 1108 42-43 0.026758929241339047 1108 44-45 0.8878128450966081 1108 46-47 -0.9929849687779253 1108 48-49 1.0630015862054911 1108 50-51 -0.06318094272678465 1108 52-53 -0.9155580860561621 1108 54-55 -0.03192197871273805 1108 56-57 -1.831880285117002 1108 58-59 -0.46068756657539467 1108 60-61 1.1703491108987265 1108 62-63 -0.7195011525008432 1108 64-65 0.6781262998057613 1108 66-67 -1.670620529226813 1108 68-69 -1.370405954511373 1108 70-71 -1.7555763160134212 1108 72-73 -0.6314350322492928 1108 74-75 -0.412724004932727 1108 76 -1.2293998837764555 1109 1 -1.8083497237807151 1109 2 -0.5080437775459892 1109 3 -1.4691348791377052 1109 4 -0.8552016902568518 1109 5 -1.783482232354654 1109 6 -1.503237590146938 1109 7 -1.0372658569471405 1109 8 -1.784611017335795 1109 9 -2.476607877633363 1109 10-11 -0.04893089978650522 1109 12-13 2.0716331506555754 1109 14-15 -0.13306090068811827 1109 16-17 -1.3811058699077172 1109 18-19 0.7478412147493643 1109 20-21 -1.5841872030761088 1109 22-23 -0.32202150307766075 1109 24-25 0.5866794291294468 1109 26-27 -0.5498913425694667 1109 28-29 0.26281262985317966 1109 30-31 -0.4556041600748344 1109 32-33 -0.18639197591411616 1109 34-35 0.5134587958229417 1109 36-37 -0.6832860754387013 1109 38-39 -1.7505753833029516 1109 40-41 -0.19290419115716162 1109 42-43 -1.439150161667751 1109 44-45 0.11508557236933825 1109 46-47 0.38201503122207825 1109 48-49 -0.22108932288541538 1109 50-51 1.6754554209095787 1109 52-53 0.3571691866711113 1109 54-55 0.7408052940145353 1109 56-57 -2.0705166487533653 1109 58-59 -1.2788693847572112 1109 60-61 -0.45465088910126994 1109 62-63 1.0532261202264337 1109 64-65 -1.026419154739692 1109 66-67 -1.4319841655904462 1109 68-69 -2.074951409056826 1109 70-71 -2.357849043286148 1109 72-73 -0.5746168504311129 1109 74-75 0.1327305405218162 1109 76 0.5206001162235445 1110 1 0.8871048216738302 1110 2 -0.09390236340457392 1110 3 2.3914711814683542 1110 4 -0.4445956296507916 1110 5 0.46954807067564985 1110 6 0.8129240260146773 1110 7 0.9935422238609419 1110 8 -1.0527928355176108 1110 9 2.8855133344878503 1110 10-11 1.2172307163751093 1110 12-13 1.1403200193424468 1110 14-15 0.4659289983017807 1110 16-17 1.64465170584986 1110 18-19 0.08748767939582791 1110 20-21 -0.14302558691449363 1110 22-23 -1.0944962505524103 1110 24-25 -0.09690642945641414 1110 26-27 0.9324318897537651 1110 28-29 0.04942879146933876 1110 30-31 1.3699008904302161 1110 32-33 -0.5197253092474483 1110 34-35 -0.3415917092275613 1110 36-37 1.0273199851673596 1110 38-39 -1.087191544919115 1110 40-41 1.3250251017721268 1110 42-43 1.7681730706554823 1110 44-45 0.7698835521673182 1110 46-47 2.2911059403129848 1110 48-49 -0.38876609056218214 1110 50-51 -0.3407061952520358 1110 52-53 0.7644924189943403 1110 54-55 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-0.740478128445357 2116 56-57 0.903413832530056 2116 58-59 0.30535521417326805 2116 60-61 0.4912047258719916 2116 62-63 -0.31174714180565033 2116 64-65 0.9033936794849069 2116 66-67 0.579379470773187 2116 68-69 1.328791906451194 2116 70-71 0.5245038978903231 2116 72-73 0.8899553420822564 2116 74-75 0.0037198453346540816 2116 76 2.4845038595390534 2117 1 -0.29067295610394694 2117 2 -0.5383468078490203 2117 3 -0.7696399296427572 2117 4 0.427626592571432 2117 5 0.10288140400898271 2117 6 0.16847958157023157 2117 7 -1.484235553916836 2117 8 1.0083182755934992 2117 9 -2.7089311099565947 2117 10-11 -1.2661026169582215 2117 12-13 2.093097797120226 2117 14-15 2.1687067760795564 2117 16-17 2.4168739280720786 2117 18-19 1.4347099016180493 2117 20-21 0.5319744130855035 2117 22-23 0.9027259716698133 2117 24-25 1.8114269038769208 2117 26-27 0.8352096675315437 2117 28-29 0.45220656924711733 2117 30-31 2.4004564459857747 2117 32-33 0.369163579641441 2117 34-35 -0.0054805981164527395 2117 36-37 1.2939866518340253 2117 38-39 1.1294751217475536 2117 40-41 0.19724732399435396 2117 42-43 -0.06793804045563334 2117 44-45 2.4837724410562068 2117 46-47 -1.3894496152425724 2117 48-49 -1.1276549794510764 2117 50-51 -0.6629284174742587 2117 52-53 0.31171464121656456 2117 54-55 0.01605781926706129 2117 56-57 -0.19299139622811623 2117 58-59 2.0708780899902663 2117 60-61 -1.3650044244548063 2117 62-63 1.991357433357745 2117 64-65 0.5733283200077821 2117 66-67 -0.17062052922681303 2117 68-69 -0.22267868178409955 2117 70-71 0.8782620678249629 2117 72-73 -0.5152734160876804 2117 74-75 -0.8508553180640384 2117 76 0.23777183339526076 2118 1 0.9081574532527803 2118 2 0.023056700922911233 2118 3 3.3897167955034426 2118 4 0.35891314227903237 2118 5 1.6291971934826677 2118 6 1.9082456634415799 2118 7 3.4280451478375475 2118 8 0.2787861118508097 2118 9 0.3977940362422352 2118 10-11 2.4809734064335913 2118 12-13 -0.5348261794879612 2118 14-15 -0.3232522882479252 2118 16-17 2.5148271444463504 2118 18-19 1.8082479133139557 2118 20-21 3.3865065768281966 2118 22-23 -0.2018061920728762 2118 24-25 1.8911052664500225 2118 26-27 1.0099172698707228 2118 28-29 0.7526451657383504 2118 30-31 1.1000178494945416 2118 32-33 -0.5284972390720135 2118 34-35 1.6019755422344275 2118 36-37 -0.14022387448176232 2118 38-39 -0.4377763402407524 2118 40-41 -1.0966123251284543 2118 42-43 0.8772373981408599 2118 44-45 0.8704683474889556 2118 46-47 0.09373751926035467 2118 48-49 2.5492456053442503 2118 50-51 2.4350247989000096 2118 52-53 1.5814514833218283 2118 54-55 2.1666426145886994 2118 56-57 1.5365407675145768 2118 58-59 2.0058196104581008 2118 60-61 3.8557558094633215 2118 62-63 0.1572931058723661 2118 64-65 -0.15620384373490737 2118 66-67 1.2635899970889746 2118 68-69 0.007584476110636729 2118 70-71 1.760572009345431 2118 72-73 2.069521905549749 2118 74-75 1.2924195357371318 2118 76 2.069643178424503 2119 1 -1.4816451783261684 2119 2 -2.4029301411823525 2119 3 -1.7106121518649786 2119 4 -2.440428962984125 2119 5 0.3528814040089827 2119 6 -3.1197148628742113 2119 7 -0.7759022205835038 2119 8 -2.1965428355176115 2119 9 -4.306153332178816 2119 10-11 -1.2591581725137786 2119 12-13 -2.571832758435331 2119 14-15 -1.694140446142665 2119 16-17 -1.3938899608168072 2119 18-19 -1.0774428761597292 2119 20-21 0.002460524196617797 2119 22-23 0.44786486055870256 2119 24-25 -0.47155920723418987 2119 26-27 -3.1890958880240134 2119 28-29 0.12928990258045303 2119 30-31 -1.464126887347561 2119 32-33 -1.1238919759141162 2119 34-35 1.6143110685502151 2119 36-37 -0.4091383481659747 2119 38-39 0.711072343969775 2119 40-41 -0.9642110093389817 2119 42-43 -0.5627297071222976 2119 44-45 0.2997446632784282 2119 46-47 -0.20889405968701524 2119 48-49 -0.9540438683399621 2119 50-51 -0.708067306363148 2119 52-53 -1.5476603587834354 2119 54-55 -0.013456069621827993 2119 56-57 -1.941255285117002 2119 58-59 -2.805858021120848 2119 60-61 1.5013150199896366 2119 62-63 -1.2933647888644764 2119 64-65 0.9622172088966714 2119 66-67 -1.404995529226813 2119 68-69 0.9960713182159004 2119 70-71 0.31402595671385214 2119 72-73 0.40833769502343387 2119 74-75 1.949491904158183 2119 76 -0.2379226110491821 >>END_MODULE >>Per sequence quality scores fail #Quality Count 11 3.0 12 42.0 13 218.0 14 712.0 15 1086.0 16 1481.0 17 1795.0 18 2029.0 19 1891.0 20 1596.0 21 1246.0 22 820.0 23 534.0 24 384.0 25 257.0 26 179.0 27 114.0 28 102.0 29 68.0 30 43.0 31 33.0 32 19.0 33 17.0 34 20.0 35 16.0 36 21.0 37 17.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 5.983080911447674 26.865875289944057 30.98649201801064 36.16455178059763 2 17.391304347826086 23.27884419724615 33.55490741368785 25.774944041239912 3 4.1714712066743544 26.493929322390287 43.99375975039001 25.340839720545343 4 17.900020348640034 25.56467476090348 26.046259241674015 30.489045648782476 5 4.205385606728617 49.37258359899614 21.24398019399037 25.17805060028488 6 6.029980329647969 33.22254629315607 11.191752017906802 49.55572135928916 7 4.286780166858848 35.569422776911075 10.628772977006037 49.51502407922404 8 3.5406633656650617 48.92491351827986 12.812860340500576 34.721562775554496 9 8.498948653598317 33.91440005426304 10.927219697483553 46.659431594655096 10-11 6.694702570711524 32.69009021230415 23.065183476904295 37.55002374008004 12-13 6.338816347295236 28.804476852636935 23.479735458707818 41.37697134136001 14-15 11.235840737977346 32.62226141219562 18.23238146917181 37.909516380655226 16-17 15.07495082411992 30.899409889439056 18.130638269009022 35.895001017432 18-19 11.090008817744014 29.159601166655364 16.9029369870447 42.847453028555925 20-21 9.594383775351014 30.187207488299535 17.74401410839042 42.474394627959036 22-23 17.313301227701285 30.088855728142168 14.410228583056368 38.18761446110018 24-25 12.527979380044767 36.51224309841959 14.033778742454045 36.925998779081596 26-27 10.059011056094418 36.4749372583599 19.49060571118497 33.97544597436071 28-29 13.42331954147731 31.194465169911144 23.604422437767074 31.777792850844467 30-31 13.145221461032355 30.39408532863054 24.693074679508918 31.76761853082819 32-33 9.095842094553348 36.970087499152136 21.549209794478735 32.384860611815775 34-35 9.173845214678153 34.71477989554364 20.26046259241674 35.85091229736146 36-37 10.35067489656108 30.702706369124332 23.329715797327548 35.616902936987046 38-39 11.181577697890525 25.391711320626737 27.548667164077866 35.87804381740487 40-41 8.366682493386692 28.186257885098016 24.852472359763954 38.59458726175134 42-43 4.8429763277487625 25.842772841348438 28.908634606253813 40.40561622464899 44-45 6.545479210472767 28.19304076510887 29.98711252797938 35.274367496438984 46-47 7.108699338646769 32.24351365100899 25.06020010174665 35.58758690859759 48-49 7.0609780912975655 34.9148748558638 19.697483551515973 38.32666350132266 50-51 6.959234891134776 33.04279997286848 18.842840670148544 41.155124465848196 52-53 5.9587600895340165 31.798141490877025 21.077799633724478 41.16529878586448 54-55 6.525130570440209 31.39795157023672 19.92810147188496 42.1488163874381 56-57 10.303194736485112 31.286034050057655 18.198467069117548 40.21230414433969 58-59 8.824526894119243 30.628094689004953 18.530828189649327 42.01655022722648 60-61 6.453910330326257 28.912026046259243 19.16163603065862 45.472427592755885 62-63 8.481991453571187 30.53652580885844 17.89323746862918 43.08824526894119 64-65 9.095842094553348 32.164417011463065 14.193176422709083 44.5465644712745 66-67 6.9185376110696595 38.86929390219087 12.38893033982229 41.82323814691718 68-69 7.8240520925184835 37.926473580682355 15.787153225259445 38.462321101539715 70-71 6.810011530896018 40.619276944990844 16.09577426575324 36.4749372583599 72-73 7.464559451943295 39.30000678288001 16.37726378620362 36.85816997897307 74-75 6.911754731058807 40.16143254425829 13.867598182188157 39.05921454249474 76 5.412738248660381 36.1391846978227 13.626805941802889 44.82127111171403 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 1.0 9 1.0 10 1.0 11 2.0 12 3.0 13 2.0 14 3.0 15 5.0 16 5.5 17 6.0 18 8.5 19 12.0 20 14.5 21 16.0 22 19.5 23 27.0 24 37.5 25 44.0 26 49.5 27 68.5 28 82.0 29 82.0 30 110.0 31 160.5 32 195.0 33 207.0 34 212.5 35 280.0 36 365.5 37 389.0 38 459.0 39 545.0 40 636.5 41 738.0 42 764.0 43 820.0 44 882.0 45 936.0 46 984.0 47 971.5 48 947.5 49 891.0 50 846.0 51 831.0 52 755.5 53 648.0 54 601.0 55 586.5 56 495.0 57 390.0 58 362.0 59 318.0 60 249.0 61 214.5 62 205.0 63 174.5 64 134.5 65 100.0 66 69.0 67 63.0 68 56.0 69 43.5 70 32.5 71 27.0 72 25.0 73 23.0 74 20.0 75 17.0 76 12.0 77 7.0 78 6.5 79 6.0 80 5.0 81 4.0 82 6.0 83 8.0 84 8.0 85 5.0 86 3.0 87 4.0 88 5.0 89 4.5 90 2.0 91 2.0 92 3.0 93 3.5 94 2.5 95 2.5 96 4.0 97 5.0 98 3.0 99 1.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.5765448009224716 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.003391440005426304 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.003391440005426304 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 14743.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.70155327952249 #Duplication Level Percentage of deduplicated Percentage of total 1 99.82992040274848 99.53198127925117 2 0.08844139057078712 0.1763548802821678 3 0.06803183890060549 0.20348640032557824 4 0.006803183890060549 0.02713152004341043 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.006803183890060549 0.06104592009767348 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.020348640032557826 0.0 2 0.0 0.0 0.0 0.020348640032557826 0.0 3 0.0 0.0 0.0 0.020348640032557826 0.0 4 0.0 0.0 0.0 0.020348640032557826 0.0 5 0.0 0.0 0.0 0.02713152004341043 0.0 6 0.0 0.0 0.0 0.02713152004341043 0.0 7 0.0 0.0 0.0 0.02713152004341043 0.0 8 0.0 0.0 0.0 0.02713152004341043 0.0 9 0.0 0.0 0.0 0.03391440005426304 0.0 10 0.0 0.0 0.0 0.03391440005426304 0.0 11 0.0 0.0 0.0 0.03391440005426304 0.0 12 0.0 0.0 0.0 0.03391440005426304 0.0 13 0.0 0.0 0.0 0.03391440005426304 0.0 14 0.0 0.0 0.0 0.03391440005426304 0.0 15 0.0 0.0 0.0 0.03391440005426304 0.0 16 0.0 0.0 0.0 0.03391440005426304 0.0 17 0.0 0.0 0.0 0.03391440005426304 0.0 18 0.0 0.0 0.0 0.04069728006511565 0.0 19 0.0 0.0 0.0 0.04748016007596825 0.0 20 0.0 0.0 0.0 0.04748016007596825 0.0 21 0.0 0.0 0.0 0.04748016007596825 0.0 22 0.0 0.0 0.0 0.04748016007596825 0.0 23 0.0 0.0 0.0 0.04748016007596825 0.0 24 0.0 0.0 0.0 0.04748016007596825 0.0 25 0.0 0.0 0.0 0.04748016007596825 0.0 26 0.0 0.0 0.0 0.04748016007596825 0.0 27 0.0 0.0 0.0 0.05426304008682086 0.0 28 0.0 0.0 0.0 0.05426304008682086 0.0 29 0.0 0.0 0.0 0.06104592009767347 0.0 30 0.0 0.0 0.0 0.06104592009767347 0.0 31 0.0 0.0 0.0 0.06104592009767347 0.0 32 0.0 0.0 0.0 0.06104592009767347 0.0 33 0.0 0.0 0.0 0.06782880010852609 0.0 34 0.0 0.0 0.0 0.06782880010852609 0.0 35 0.0 0.0 0.0 0.07461168011937869 0.0 36 0.0 0.0 0.0 0.0813945601302313 0.0 37 0.0 0.0 0.0 0.14244048022790476 0.0 38 0.0 0.0 0.0 0.18313776029302042 0.0 39 0.0 0.0 0.0 0.23061792036898868 0.0 40 0.0 0.0 0.0 0.2577494404123991 0.0 41 0.0 0.0 0.0 0.31201248049922 0.0 42 0.0 0.0 0.0 0.3527097605643356 0.0 43 0.0 0.0 0.0 0.36627552058604085 0.0 44 0.0 0.0 0.0 0.38662416061859867 0.0 45 0.0 0.0 0.0 0.4476700807162721 0.0 46 0.0 0.0 0.0 0.4748016007596826 0.0 47 0.0 0.0 0.0 0.5087160008139456 0.0 48 0.0 0.0 0.0 0.535847520857356 0.0 49 0.0 0.0 0.0 0.5494132808790613 0.0 50 0.0 0.0 0.0 0.569761920911619 0.0 51 0.0 0.0 0.0 0.5765448009224717 0.0 52 0.0 0.0 0.0 0.6104592009767347 0.0 53 0.0 0.0 0.0 0.6172420809875874 0.0 54 0.0 0.0 0.0 0.6782880010852608 0.0 55 0.0 0.0 0.0 0.6986366411178186 0.0 56 0.0 0.0 0.0 0.7189852811503764 0.0 57 0.0 0.0 0.0 0.7528996812046395 0.0 58 0.0 0.0 0.0 0.7732483212371973 0.0 59 0.0 0.0 0.0 0.7868140812589025 0.0 60 0.0 0.0 0.0 0.813945601302313 0.0 61 0.0 0.0 0.0 0.8410771213457234 0.0 62 0.0 0.0 0.0 0.8614257613782812 0.0 63 0.0 0.0 0.0 0.8817744014108391 0.0 64 0.0 0.0 0.0 0.9156888014651021 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGTAACA 15 0.0021236085 70.4726 1 CGCCGAC 20 0.006819592 52.4949 22 TTATACA 100 1.0921212E-8 31.49694 2 TCTGACG 90 1.5686965E-7 31.108088 11 TGCCGAC 60 4.754478E-4 29.163832 16 GCTGCCG 60 4.754478E-4 29.163832 17 CTGACGC 85 3.6241272E-6 28.820728 12 TATACAC 125 2.9922376E-9 27.99728 3 ACACATC 125 2.9922376E-9 27.99728 6 CTTATAC 90 5.3592903E-6 27.406012 1 ATCTGAC 115 4.2553438E-8 27.388643 10 CTGCCGA 70 0.001170557 24.99757 15 ACATCTG 105 1.870474E-5 23.331066 8 GACGCTG 75 0.0017497583 23.331064 11 CATCTGA 110 2.6805672E-5 22.270563 9 CGCTGCC 80 0.002546581 21.872875 13 GCCGACG 80 0.002546581 21.872875 17 CCGACGA 80 0.002546581 21.872875 18 TACACAT 135 5.3918047E-6 20.738724 5 TGACGCT 85 0.0036202583 20.586235 10 >>END_MODULE