FastQCFastQC Report
Wed 25 May 2016
SRR1780464_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780464_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14743
Sequences flagged as poor quality0
Sequence length76
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGT414528.11503764498406No Hit
ATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTCTT287119.473648511157837Illumina PCR Primer Index 1 (95% over 22bp)
CTTTACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTC9406.3759072102014525No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCG4943.350742725361188No Hit
CTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTC4433.0048158448077054No Hit
TATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTCT2591.7567659228108254Illumina PCR Primer Index 1 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTGGTCACAATATCGTATGCCGT2071.40405616224649No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCC1200.813945601302313No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCC1160.7868140812589025No Hit
CTTAATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCG1010.6850708810961134No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGTCACAAACTCGTATGCCGT970.657939361052703No Hit
CTTAACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTC970.657939361052703No Hit
ATACACATCTCCGAGCCCACGAGACTGGTCACAATATCGTATGCCGTCTT910.6172420809875874No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGTCACAACCTCGTATGCCGT900.6104592009767348No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCC890.6036763209658821No Hit
CTTATAACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCG660.4476700807162721No Hit
ATACACATCTCCGAGCCCACGAGACTGGTCACAAACTCGTATGCCGTCTT640.4341043206945669No Hit
TTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTC640.4341043206945669No Hit
ATACACATCTCCGAGCCCACGAGACTGGTCACAACCTCGTATGCCGTCTT570.38662416061859867No Hit
CTTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCG560.3798412806077461No Hit
CTTATCACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTC520.3527097605643356No Hit
CTTATACACATCTCCGAGCCCACGCGACTGGTCACAATCTCGTATGCCGT510.345926880553483No Hit
CTTATTACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCG490.3323611205317778No Hit
CTTATACACATCTCCGAGCCCACGAGCCTGGTCACAATCTCGTATGCCGT440.29844672047751475No Hit
CTTACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTCT430.29166384046666216Illumina PCR Primer Index 1 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACTGGTCACAATATCGTATGCCG390.2645323204232517No Hit
ATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTCTTCTGCTT390.2645323204232517Illumina PCR Primer Index 1 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTCTTCT360.2441836803906939Illumina PCR Primer Index 1 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACTGGTCACCATCTCGTATGCCGT350.2374008003798413No Hit
ATACACATCTCCGAGCCCACGAGACTGGTCACACTCTCGTATGCCGTCTT340.23061792036898868No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGTAACAATCTCGTATGCCGT330.22383504035813606No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGTCCCAATCTCGTATGCCGT330.22383504035813606No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGGTCACAAACTCGTATGCCG290.19670352031472563No Hit
ATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTCTGCCGTCTT250.1695720002713152No Hit
CTTATACACATATCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGT250.1695720002713152No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCAGT250.1695720002713152No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTAGTATGCCGT240.1627891202604626No Hit
TCTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGT230.15600624024961No Hit
CTTTACACATCTCCGAGCCCACGAGACTGGTCACAACCTCGTATGCCGTC230.15600624024961No Hit
CTTTACACATCTCCGAGCCCACGAGACTGGTCACAATATCGTATGCCGTC230.15600624024961No Hit
ATACACATCTCCGAGCCCACGAGACTGGTCACCATCTCGTATGCCGTCTT230.15600624024961Illumina PCR Primer Index 1 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACTGGTAACAATCTCGTATGCCGTCTT220.14922336023875737TruSeq Adapter, Index 4 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGAATGGTCACAATCTCGTATGCCGTCTT220.14922336023875737TruSeq Adapter, Index 1 (95% over 22bp)
CTTATACACATCTCCGAGCACACGAGACTGGTCACAATCTCGTATGCCGT210.14244048022790476No Hit
CTTATACACATCTCCGAGCCCACGAGAATGGTCACAATCTCGTATGCCGT210.14244048022790476No Hit
TCTTTACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGT190.12887472020619956No Hit
CTTTACACATCTCCGAGCCCACGAGACTGGTCACAAACTCGTATGCCGTC190.12887472020619956No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGGTCACAACCTCGTATGCCG180.12209184019534695No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTCTGCCGT180.12209184019534695No Hit
ATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTATT180.12209184019534695No Hit
CTTATATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCC180.12209184019534695No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGTCACACTCTCGTATGCCGT170.11530896018449434No Hit
CTATACACATCTCCGAGCCCACGAGACTGGTCACAATATCGTATGCCGTC160.10852608017364172No Hit
CTTACACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGT150.10174320016278912No Hit
CTTATACACATCTCCGAGCCCAAGAGACTGGTCACAATCTCGTATGCCGT150.10174320016278912No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCTGG150.00218134870.0000125
TAAAAAA150.00218134870.0000161
TTATTAC150.00218134870.000012
GAGCCTG150.00218134870.0000124
CACGAGC150.00218134870.0000121
ATTACAC150.00218134870.000014
CTTATTA150.00218134870.000011
CGAGCCT150.00218134870.0000123
TATTACA150.00218134870.000013
GCCTGGT150.00218134870.0000126
CCTGGTC150.00218134870.0000127
CTTTACA1100.070.01
CTTACAC207.671557E-570.01
TTTACAC1100.070.02
CTATACA501.2732926E-1162.9999961
TCTTATA1300.056.538461
CTTATAC6750.056.01
GAAAAAC252.313972E-455.99999660
TTATACA6900.055.2898562
TTACACA1450.053.1034473
Produced by FastQC (version 0.11.5)