FastQCFastQC Report
Wed 25 May 2016
SRR1780463_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780463_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85625
Sequences flagged as poor quality0
Sequence length50
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTGACGCTGCCGACGAGACCCAATGTGTAGATCTCGGTG1060.1237956204379562No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTACAC1050.039.7281382
CTTTACA1100.037.9444771
CTTATAA301.3022075E-436.613091
GATATCG301.3066983E-436.59170541
AGATATC250.002323839635.25158340
GTTATAC250.002357482435.1485671
ACGCATC250.002364257135.1280366
TTACACA1200.032.9325333
CTATACA353.2206214E-431.3826521
ATATCGG353.231691E-431.3643242
CTTATAC5700.029.6758751
TTATACA5500.029.5394862
TTATAAC300.00577307429.2733632
AAACATC300.00577307429.2733635
ATAACAC300.00577307429.2733634
TATAACA300.00577307429.2733633
TATACAC5900.027.9089283
TAAGAGT407.0383964E-427.4598181
AACATCT407.0625084E-427.443786
TTTATAC407.0625084E-427.443783
Produced by FastQC (version 0.11.5)