FastQCFastQC Report
Wed 25 May 2016
SRR1780463_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780463_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85625
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGT3333438.93021897810219No Hit
ATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTCTT2190225.57897810218978RNA PCR Primer, Index 32 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTC62467.294598540145985No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCG47035.492554744525548No Hit
CTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTC32193.7594160583941605No Hit
TATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTCT19782.31007299270073No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCC12271.432992700729927No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCC10381.2122627737226277No Hit
CTTAATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCG8420.9833576642335766No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCC7930.9261313868613138No Hit
CTTAACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTC6160.7194160583941606No Hit
CTTATAACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCG5760.6727007299270074No Hit
TTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTC4950.5781021897810219No Hit
CTTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCG4110.48No Hit
CTTATTACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCG3750.43795620437956206No Hit
CTTATCACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTC3430.40058394160583943No Hit
CTTATACACATCTCCGAGCCCACGAGACCAATCTTATCTCGTATGCCGTC3010.3515328467153285No Hit
ACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTCTTCT2580.3013138686131387RNA PCR Primer, Index 32 (95% over 23bp)
CTTACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTCT2540.29664233576642335No Hit
ATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTCTTCTGCTT2360.2756204379562044TruSeq Adapter, Index 8 (96% over 26bp)
TCTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGT2200.2569343065693431No Hit
ATACACATCTCCGAGCCCACGAGACCAATCTTATCTCGTATGCCGTCTTC1820.21255474452554746TruSeq Adapter, Index 10 (95% over 23bp)
TCTTTACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGT1580.18452554744525548No Hit
CTTATACACATCTCCGAGCCCACGAGACCAATCTTAATATCGTATGCCGT1570.18335766423357663No Hit
CTTATATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCC1520.17751824817518247No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGC1410.16467153284671532No Hit
ATACACATCTCCGAGCCCACGAGACCAATCTTAATATCGTATGCCGTCTT1150.1343065693430657No Hit
CCTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCG1100.12846715328467154No Hit
TCTTAACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGT970.11328467153284671No Hit
TACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTCTTC880.10277372262773722TruSeq Adapter, Index 8 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATCA408.123607E-944.0210381
CTTACAC302.4920319E-644.0210381
CTTATAT207.7972637E-444.0210381
CTTATAA800.044.0210381
TATATAC207.8197574E-443.9953273
TTATATA207.8197574E-443.9953272
TTATAAC700.043.9953272
ATAACAC700.043.9953274
ATATACA207.8197574E-443.9953274
CTTTACA6700.043.0354961
TTTACAC6700.043.0103572
CCGTCTT24050.041.891644
TCTTTAT900.041.5754241
TATAACA750.041.0623053
CTATACA3700.040.4517671
TTACACA7400.038.941813
CTTATTA404.0500345E-738.518411
ATTACAC404.068679E-738.495914
GCCGTCT26500.037.85258543
CTTATAC41600.037.3543931
Produced by FastQC (version 0.11.5)