FastQCFastQC Report
Wed 25 May 2016
SRR1780462_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780462_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86737
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGGCA200.006932743452.49567862
ATGGTTG200.006932743452.49567818
GGACACA200.006932743452.49567824
AAATGAG200.006932743452.49567829
TACTCGG305.851588E-446.6628220
GAAACAC350.001214780440.2287031
TGTGCTA350.001249865439.99670416
CGAGGAG400.002408131334.9971249
ACTTGAA450.004288465331.10854511
TTTCGTT450.004288465331.10854521
TTACTCG450.004288465331.10854519
GTCGCCC500.007177117327.99769437
TTGGTAG500.007177117327.99769467
TGTACTG500.007177117327.9976945
AGGAACA700.001218013224.9979423
GCATGAG750.001820332423.33141155
TTTTCGT750.001820332423.33141120
TGGTCAA750.001820332423.33141117
CATGAGT800.002648766921.873256
CTTTACA900.00506828819.555621
Produced by FastQC (version 0.11.5)