##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780462_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 86737 Sequences flagged as poor quality 0 Sequence length 76 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.4764056861547 34.0 23.0 34.0 12.0 34.0 2 29.598706434393627 34.0 31.0 34.0 12.0 34.0 3 29.753738312369578 34.0 31.0 34.0 11.0 34.0 4 30.037884639773107 34.0 31.0 34.0 11.0 34.0 5 29.894843031232345 34.0 31.0 34.0 11.0 34.0 6 32.322688126174526 37.0 31.0 38.0 11.0 38.0 7 31.45927343578865 37.0 27.0 38.0 11.0 38.0 8 31.073647924184606 36.0 27.0 38.0 11.0 38.0 9 31.60993578288389 37.0 27.0 38.0 12.0 38.0 10-11 31.84038530269666 37.0 29.5 38.0 11.0 38.0 12-13 32.15658254262887 37.0 31.0 38.0 11.0 38.0 14-15 31.80522153175692 37.0 29.0 38.0 11.0 38.0 16-17 31.645848945663328 37.0 27.5 38.0 11.0 38.0 18-19 31.67056734726818 37.0 29.0 38.0 11.0 38.0 20-21 31.75984297358682 37.0 28.5 38.0 11.0 38.0 22-23 31.62489479691481 37.0 27.5 38.0 11.0 38.0 24-25 32.00300909646402 37.0 31.0 38.0 11.0 38.0 26-27 32.04844530016025 37.0 31.0 38.0 11.0 38.0 28-29 32.08628382351246 37.0 31.0 38.0 11.0 38.0 30-31 31.930145151434797 37.0 31.0 38.0 11.0 38.0 32-33 31.780082317811313 37.0 29.0 38.0 11.0 38.0 34-35 31.74094100556856 37.0 27.0 38.0 11.0 38.0 36-37 31.67685647416904 37.0 27.0 38.0 11.0 38.0 38-39 32.025986603179724 37.0 31.0 38.0 11.0 38.0 40-41 31.611036812432985 37.0 27.0 38.0 11.0 38.0 42-43 31.93092336603756 37.0 30.5 38.0 11.0 38.0 44-45 31.114662716026608 37.0 26.5 38.0 11.0 38.0 46-47 31.763837808547677 37.0 27.0 38.0 11.0 38.0 48-49 31.578559323010943 37.0 27.0 38.0 11.0 38.0 50-51 31.499579187659247 37.0 27.0 38.0 11.0 38.0 52-53 31.48586531699275 37.0 27.0 38.0 11.0 38.0 54-55 31.29409594521369 37.0 27.0 38.0 11.0 38.0 56-57 31.26870885550572 37.0 26.5 38.0 11.0 38.0 58-59 31.128612933350244 36.5 26.5 38.0 11.0 38.0 60-61 31.184725088485884 37.0 26.5 38.0 11.0 38.0 62-63 31.250210406170375 37.0 27.0 38.0 11.0 38.0 64-65 31.283610224010516 37.0 27.0 38.0 11.0 38.0 66-67 31.303572869709583 37.0 27.0 38.0 11.0 38.0 68-69 30.840892583326607 36.5 26.0 38.0 11.0 38.0 70-71 30.747339658969068 36.0 25.0 38.0 11.0 38.0 72-73 30.83880581528068 36.5 25.5 38.0 11.0 38.0 74-75 30.54798413595121 36.0 25.0 38.0 11.0 38.0 76 29.966104430635138 35.0 24.0 37.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -2.8355613207035013 1101 2 -1.269542931748024 1101 3 -0.9884264303296959 1101 4 -2.242165116393995 1101 5 -1.4526393321993112 1101 6 -2.4773351592048876 1101 7 -1.303383682735884 1101 8 -1.48545236594504 1101 9 -1.0268954608381016 1101 10-11 -0.9877299040190302 1101 12-13 -0.9001313172331038 1101 14-15 -0.9787324759373739 1101 16-17 -2.3995366050161167 1101 18-19 -0.20782517363160835 1101 20-21 -0.6882818093825982 1101 22-23 -1.1471925113883579 1101 24-25 -1.3245317618593546 1101 26-27 -1.4088212231052282 1101 28-29 -1.8633357011535665 1101 30-31 -1.3668791778644476 1101 32-33 -0.9567709266323803 1101 34-35 -1.3997637862350736 1101 36-37 -1.084830411934817 1101 38-39 -1.1514816658137903 1101 40-41 -0.3835033073598595 1101 42-43 -0.9131522880321619 1101 44-45 -0.3232293703262421 1101 46-47 -1.3916304362829308 1101 48-49 -1.9980376432246452 1101 50-51 -2.2063943438776725 1101 52-53 -1.7799775250548642 1101 54-55 -2.698332628832304 1101 56-57 -1.4001451941735965 1101 58-59 -1.1707741188697725 1101 60-61 -0.7833497941043248 1101 62-63 -1.973459695676624 1101 64-65 -1.7099179189699072 1101 66-67 -1.6354061489270286 1101 68-69 -1.1208866608148718 1101 70-71 -0.7009337256067347 1101 72-73 -0.8953269551802698 1101 74-75 -2.0251938229955933 1101 76 -3.181900011222588 1102 1 0.17628373079672244 1102 2 -0.6541376473772225 1102 3 0.07720276805812176 1102 4 0.4026400784111992 1102 5 1.1676472777424713 1102 6 0.8950786338985637 1102 7 1.3522141676895494 1102 8 1.9158239439519598 1102 9 1.137255905037403 1102 10-11 0.2391957565273195 1102 12-13 -3.5015531712411665 1102 14-15 -0.8139093680108154 1102 16-17 -1.6760032418275799 1102 18-19 -0.4650352049795714 1102 20-21 -0.10216447843767895 1102 22-23 -0.30423460722355244 1102 24-25 0.14920312394449553 1102 26-27 -5.476521626150458 1102 28-29 -0.5168511512207417 1102 30-31 0.09158477197880899 1102 32-33 -5.4934816297223925 1102 34-35 0.09136921869103176 1102 36-37 0.7103554366993023 1102 38-39 0.536225454651948 1102 40-41 0.41526516554654336 1102 42-43 -0.1749413610281323 1102 44-45 -4.608798111929463 1102 46-47 0.05932119828939264 1102 48-49 0.2399717611640675 1102 50-51 -5.223176699453131 1102 52-53 -1.7429869294435782 1102 54-55 0.5572876129948341 1102 56-57 -0.889889932194194 1102 58-59 0.5488967275252108 1102 60-61 0.28577246294896597 1102 62-63 -4.328318629845903 1102 64-65 -0.17099046711141952 1102 66-67 0.45577163880248506 1102 68-69 -6.46779664738003 1102 70-71 -0.3178392338915508 1102 72-73 0.9154657004399738 1102 74-75 -5.685292345163081 1102 76 -2.47616781238694 1103 1 0.41904810927538705 1103 2 0.024897603086600384 1103 3 0.37877976249225753 1103 4 -0.2829814429246085 1103 5 -0.2633360246694032 1103 6 -1.0069621824279693 1103 7 0.45861490980458086 1103 8 1.5329853539705134 1103 9 0.27964922600215303 1103 10-11 0.35723842814142515 1103 12-13 -0.8374993679016498 1103 14-15 -0.26001326411471126 1103 16-17 -0.6510496240168173 1103 18-19 -1.4035324220482188 1103 20-21 -1.0324056658217238 1103 22-23 -0.6138357203961 1103 24-25 -0.48280058663064196 1103 26-27 -0.8480893441467501 1103 28-29 -0.2012667356363238 1103 30-31 -0.4587862855351048 1103 32-33 -4.452618921002539 1103 34-35 -0.6984266996763147 1103 36-37 -0.626843079070639 1103 38-39 -0.44549996278775694 1103 40-41 -0.014326671188150897 1103 42-43 -0.35875397698360345 1103 44-45 -4.322666386512022 1103 46-47 -0.7568661857551326 1103 48-49 -0.8083120978340723 1103 50-51 -0.4383436753343908 1103 52-53 -0.8860296010576789 1103 54-55 -0.5588533888604523 1103 56-57 0.3349709212380887 1103 58-59 0.17658689450109222 1103 60-61 -0.15365239790446594 1103 62-63 0.11323981171253905 1103 64-65 -0.3781797064435146 1103 66-67 -0.8954342981177383 1103 68-69 -1.948965478548864 1103 70-71 -0.009026617936079617 1103 72-73 -0.3006753014153176 1103 74-75 -2.034271936999815 1103 76 -0.4937930442978882 1104 1 -1.944372195555406 1104 2 0.28226434725262095 1104 3 0.639745921107604 1104 4 0.21654494991100748 1104 5 -0.3516852884255357 1104 6 -1.190997315468529 1104 7 0.4032114826109172 1104 8 1.5802082937793465 1104 9 0.06266825639912099 1104 10-11 0.05381984551850039 1104 12-13 -1.0736571221425848 1104 14-15 -0.31830138949144526 1104 16-17 -0.5204029349614459 1104 18-19 0.43279755297976763 1104 20-21 0.4251849653674604 1104 22-23 -0.1286208749642519 1104 24-25 0.23430093752332226 1104 26-27 0.24819068685299328 1104 28-29 -0.829777886932284 1104 30-31 -0.8996887224592385 1104 32-33 -0.9299891095843016 1104 34-35 0.45508229885981066 1104 36-37 0.05180154333528719 1104 38-39 -0.007637222755029427 1104 40-41 -0.1916968597699089 1104 42-43 0.07550934859979463 1104 44-45 -2.9525553041043757 1104 46-47 -0.2738299332794618 1104 48-49 0.6147224324337266 1104 50-51 0.8468367259400402 1104 52-53 -0.3821890775064851 1104 54-55 -0.6098393528663095 1104 56-57 0.6062358829189591 1104 58-59 0.5577287183192254 1104 60-61 -0.2415194434568626 1104 62-63 0.18418616493737616 1104 64-65 -0.5342720167853727 1104 66-67 0.5465272966467474 1104 68-69 -3.207976931231201 1104 70-71 -1.3103785511144146 1104 72-73 -0.35841232027349434 1104 74-75 -1.6684147080322802 1104 76 -0.7324393889116827 1105 1 -0.4789110743980842 1105 2 0.7840812765559875 1105 3 -0.07428146199753627 1105 4 0.6353859040141678 1105 5 0.952990505942843 1105 6 0.8134459808373364 1105 7 0.8422883791923361 1105 8 0.4554343335623514 1105 9 1.1735267770225626 1105 10-11 1.2837690403863213 1105 12-13 -0.6987238576975656 1105 14-15 0.18692551139549707 1105 16-17 1.2407871106770543 1105 18-19 0.35156961876811366 1105 20-21 0.19208413009664227 1105 22-23 1.0392255040937037 1105 24-25 0.7620973725530291 1105 26-27 0.7253800436083537 1105 28-29 -0.11351163359550753 1105 30-31 0.41060146956693444 1105 32-33 1.0004423034872545 1105 34-35 0.8076957493032744 1105 36-37 1.1119324311334466 1105 38-39 0.7524592208857186 1105 40-41 0.9938365941179725 1105 42-43 1.0187970434245557 1105 44-45 1.5446805522634897 1105 46-47 1.3125215693469094 1105 48-49 0.9610756572679655 1105 50-51 0.9596155083390734 1105 52-53 -0.19623368269033037 1105 54-55 -0.148649307227803 1105 56-57 0.1104811253197191 1105 58-59 0.7684236291949702 1105 60-61 0.495327193932269 1105 62-63 0.5540561382431513 1105 64-65 1.1014121302911803 1105 66-67 0.7923208039230794 1105 68-69 0.7979732969613416 1105 70-71 1.4358713412475943 1105 72-73 0.35646755572754074 1105 74-75 -1.4261086716936937 1105 76 0.20824777202864198 1106 1 -0.9467008318104817 1106 2 0.018332335470120142 1106 3 -0.06224834686469194 1106 4 -0.20173384954763307 1106 5 0.5568411027853557 1106 6 -0.5219024981769103 1106 7 0.7768282328696579 1106 8 0.2707982149639676 1106 9 1.0261066769070446 1106 10-11 0.5818629949492795 1106 12-13 0.04192881846724106 1106 14-15 -0.2655388705837147 1106 16-17 0.22854221271787623 1106 18-19 -0.08664755489380482 1106 20-21 -0.12390547329188095 1106 22-23 0.3159197667044076 1106 24-25 0.5879252508741715 1106 26-27 0.13098266201420472 1106 28-29 -0.6323314256632742 1106 30-31 -0.07157989354434591 1106 32-33 0.5137653685623462 1106 34-35 0.7432560111438669 1106 36-37 1.1586401708330953 1106 38-39 0.5627691939315369 1106 40-41 0.030359505169187173 1106 42-43 0.954261040344079 1106 44-45 1.7894437405576689 1106 46-47 0.7739867214454783 1106 48-49 0.5236767354350853 1106 50-51 0.7051680689859801 1106 52-53 0.7667043227005017 1106 54-55 0.3702378702847149 1106 56-57 0.8860105823855662 1106 58-59 0.6659207412473407 1106 60-61 0.7890314680947661 1106 62-63 1.281501267238145 1106 64-65 1.2991210251698817 1106 66-67 0.7366893060409048 1106 68-69 0.9730695618824043 1106 70-71 0.26286402511358986 1106 72-73 0.6845480332015512 1106 74-75 -0.8433112130876097 1106 76 0.17752000922540745 1107 1 -0.6650269665380719 1107 2 -0.6839295422293574 1107 3 -0.28959168247126854 1107 4 -0.3299448065833239 1107 5 -0.2574348690264223 1107 6 0.0886529310873172 1107 7 0.2138972637099954 1107 8 1.0245680111297624 1107 9 -0.14520119609439774 1107 10-11 -0.15298204559023887 1107 12-13 -0.10563312742955588 1107 14-15 -0.5927155016362242 1107 16-17 0.4915944215532164 1107 18-19 0.15729046132199898 1107 20-21 0.07111043941555195 1107 22-23 -0.6921407773586417 1107 24-25 -0.2648348461175729 1107 26-27 -0.017403334803255177 1107 28-29 -0.7344853242765979 1107 30-31 -0.43570621013144617 1107 32-33 0.3277761285835652 1107 34-35 -0.06766692588727707 1107 36-37 -0.44077636322767333 1107 38-39 0.23065772920470096 1107 40-41 0.36727319767506117 1107 42-43 -0.28730901345602433 1107 44-45 -0.15442607834056332 1107 46-47 0.13072499529560488 1107 48-49 0.22630801527140676 1107 50-51 0.0725242863080986 1107 52-53 0.5278053305053056 1107 54-55 0.41219816429093115 1107 56-57 -0.1515547733771072 1107 58-59 0.23817201047519276 1107 60-61 -0.13392450674800216 1107 62-63 -0.32313425598682954 1107 64-65 -0.24465786397158595 1107 66-67 0.24062012365097019 1107 68-69 0.9905640682096006 1107 70-71 -0.2692266015439806 1107 72-73 -0.3364510575008808 1107 74-75 -0.3519590118297451 1107 76 -0.6593368521824878 1108 1 -0.4937592183800845 1108 2 0.44371489378240625 1108 3 -0.254901741605444 1108 4 -0.43676579130963233 1108 5 -0.02197333786239497 1108 6 -0.04114183854238718 1108 7 0.12848474750343897 1108 8 0.722588439299134 1108 9 -0.6042974235237999 1108 10-11 0.34601393834550365 1108 12-13 0.5213709518798098 1108 14-15 0.5834063084387218 1108 16-17 -0.4210640864947237 1108 18-19 0.14155033546423468 1108 20-21 0.7658025938600979 1108 22-23 -0.37021528009399063 1108 24-25 -0.6782127672509226 1108 26-27 -0.3682002231440187 1108 28-29 0.46611234197897744 1108 30-31 0.6059011642479604 1108 32-33 0.9045319708502575 1108 34-35 0.5319203997933961 1108 36-37 -0.23513489973591106 1108 38-39 1.2398045527192352 1108 40-41 0.9774698899559979 1108 42-43 0.985348545915322 1108 44-45 1.3422219310197114 1108 46-47 0.7899761732357113 1108 48-49 1.098758446919259 1108 50-51 1.1259822125010537 1108 52-53 0.9027539438563501 1108 54-55 0.8851687869390013 1108 56-57 1.2190220219933536 1108 58-59 -0.7121805809930422 1108 60-61 1.2190938659554114 1108 62-63 0.10052890057643182 1108 64-65 0.9266652236545099 1108 66-67 0.333795976669343 1108 68-69 1.1253314843307791 1108 70-71 1.8004141662516098 1108 72-73 0.9752366381636648 1108 74-75 1.5962037178290451 1108 76 1.0377190881141338 1109 1 -0.18387222203705633 1109 2 -0.8757428204468312 1109 3 -0.11646402539946621 1109 4 -1.04013283904791 1109 5 -0.406449336939918 1109 6 -0.6982268054319825 1109 7 -0.9267321997505391 1109 8 0.4446752182007252 1109 9 -0.46645272942436833 1109 10-11 -0.07135007839089624 1109 12-13 -0.660102049141507 1109 14-15 -0.17208738246498 1109 16-17 -0.6020017203365171 1109 18-19 -0.4285004008715454 1109 20-21 0.16147188519868294 1109 22-23 -0.19648451213036466 1109 24-25 0.08452073520353309 1109 26-27 -0.1295170616772765 1109 28-29 -0.11901927598300688 1109 30-31 -0.2751896669713574 1109 32-33 0.29852103288696696 1109 34-35 -0.39231278967716676 1109 36-37 -0.1812193065490746 1109 38-39 -0.2176543475542161 1109 40-41 -0.4192536629053336 1109 42-43 -0.04467319822858684 1109 44-45 -1.0950000898678418 1109 46-47 -1.0299427804097299 1109 48-49 -0.897979931494735 1109 50-51 0.19320785741259883 1109 52-53 0.1785421690729656 1109 54-55 -0.16424148552171047 1109 56-57 0.13399747746727542 1109 58-59 -0.36526264866345315 1109 60-61 0.025768659221313328 1109 62-63 -0.6169454841631357 1109 64-65 -0.32010610043207066 1109 66-67 -0.36328503673954415 1109 68-69 0.6413513176256771 1109 70-71 -0.300094844389843 1109 72-73 -0.30857385832761963 1109 74-75 1.383745311740686 1109 76 0.14357353413264917 1110 1 -0.7594305549175644 1110 2 -1.0757826690222423 1110 3 -1.2641392232838697 1110 4 -0.4369703111991896 1110 5 -0.7184999084047128 1110 6 -0.37418543536550786 1110 7 -0.2306862652108812 1110 8 -0.5835267053986897 1110 9 -2.6153414975599993 1110 10-11 -2.401821559489992 1110 12-13 -1.6884579331459264 1110 14-15 -1.4279786320800802 1110 16-17 -1.576652592476929 1110 18-19 -1.2163339062782725 1110 20-21 -1.2844155606887604 1110 22-23 -1.1539099318988768 1110 24-25 -1.322441897700525 1110 26-27 -1.8413484659772976 1110 28-29 -1.5099247442943309 1110 30-31 -1.3445624141683723 1110 32-33 -0.6933734046141637 1110 34-35 -1.3367693094474937 1110 36-37 -0.6794714031275362 1110 38-39 -1.0181035496770576 1110 40-41 -1.5459902457088646 1110 42-43 -1.2094651705519404 1110 44-45 -0.5602050383363881 1110 46-47 -0.9269342129660174 1110 48-49 -0.5190109228186124 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-0.8207853311723952 2116 40-41 -1.3720229700466753 2116 42-43 -0.8096100821375316 2116 44-45 0.33885211919534086 2116 46-47 -0.6865185551774786 2116 48-49 -0.8417693790516445 2116 50-51 -0.5668978088522074 2116 52-53 -0.31972036549904814 2116 54-55 -0.5579281034920704 2116 56-57 -0.5063383143205442 2116 58-59 -1.269400653060309 2116 60-61 -2.1416928683299226 2116 62-63 -0.6993509873189403 2116 64-65 -0.5410212837524035 2116 66-67 -0.8201143396258672 2116 68-69 -0.9229969555464379 2116 70-71 -3.1725772924431688 2116 72-73 -0.5213601855384553 2116 74-75 -1.424699124824098 2116 76 -2.0700815257921796 2117 1 -0.46418867888966275 2117 2 -0.07120791335578502 2117 3 -0.14655666028646053 2117 4 -0.14422375644390328 2117 5 -0.808652841352405 2117 6 -1.0996811914289495 2117 7 -1.6735301753037 2117 8 -1.9020204387395836 2117 9 0.2759418915399827 2117 10-11 -0.7271301664579894 2117 12-13 0.43351034602759597 2117 14-15 -0.758391305287514 2117 16-17 -0.27800800562273764 2117 18-19 0.15725538652499793 2117 20-21 -0.3909298615262067 2117 22-23 -0.5745700577991819 2117 24-25 0.38360169480595374 2117 26-27 0.5236867898876483 2117 28-29 0.22923856692138145 2117 30-31 -0.07445333838244395 2117 32-33 0.05710392009896381 2117 34-35 -0.1929102572914978 2117 36-37 -1.254888707802479 2117 38-39 -0.1439849463008116 2117 40-41 -1.6718527383835848 2117 42-43 -1.7699294515402357 2117 44-45 -0.04785130764204837 2117 46-47 -0.8531142919845252 2117 48-49 -0.7623803626946035 2117 50-51 0.060239338657229524 2117 52-53 0.01056606777753899 2117 54-55 -0.8652610174140598 2117 56-57 -0.6428474550207142 2117 58-59 -1.231006011657005 2117 60-61 -0.1892374616242769 2117 62-63 -0.45606773663431 2117 64-65 -0.37594282915985033 2117 66-67 -1.2098893377755253 2117 68-69 -0.8999324155420005 2117 70-71 0.26896108369479066 2117 72-73 0.3440087730878503 2117 74-75 -0.5193534805342601 2117 76 -0.7082837314024246 2118 1 0.46789496509088124 2118 2 -0.022356420474043404 2118 3 0.23607194765900985 2118 4 0.31997193354793296 2118 5 1.0999458734557024 2118 6 0.7601192843050626 2118 7 0.893640627408697 2118 8 -1.6525796037198788 2118 9 -0.6835497121096772 2118 10-11 0.03910336923981461 2118 12-13 1.189670036445456 2118 14-15 -0.41483324088590834 2118 16-17 -0.04178299053415202 2118 18-19 -0.6406819159921362 2118 20-21 -1.8161703912240803 2118 22-23 -0.4232848659079629 2118 24-25 -0.12784787383820628 2118 26-27 0.4413830301688293 2118 28-29 0.2644127068723847 2118 30-31 0.012501253870901508 2118 32-33 0.4115257612606058 2118 34-35 0.6934017390162346 2118 36-37 0.37247664882051623 2118 38-39 0.30385294910870897 2118 40-41 -0.2668486555916658 2118 42-43 0.10393890504725789 2118 44-45 0.7082196264297345 2118 46-47 0.08816450944977916 2118 48-49 0.027684251925339964 2118 50-51 0.3093251482926185 2118 52-53 -0.32195958280647474 2118 54-55 0.7732521362764295 2118 56-57 0.897323667214522 2118 58-59 0.8496912581977938 2118 60-61 -0.3981521490244262 2118 62-63 0.8066776746625983 2118 64-65 0.5788801906860641 2118 66-67 -1.0236075186254538 2118 68-69 1.170895148628837 2118 70-71 0.18681708539891062 2118 72-73 -1.1669437600182704 2118 74-75 0.8756832645930182 2118 76 -0.2846674428377902 2119 1 0.989920094433085 2119 2 1.1867714435318675 2119 3 0.7408391316944858 2119 4 0.7208218965930158 2119 5 1.0994654595606548 2119 6 1.840533179353109 2119 7 -1.4068767414013585 2119 8 1.0703693984974159 2119 9 0.45998317776467346 2119 10-11 0.8891957565273252 2119 12-13 0.9052650105770184 2119 14-15 0.34972699562554865 2119 16-17 0.6808149399906043 2119 18-19 0.1758738859295228 2119 20-21 -0.07716447843768393 2119 22-23 1.2071290291400807 2119 24-25 0.40829403303540346 2119 26-27 0.6189329193040862 2119 28-29 1.028603394233805 2119 30-31 0.8143120447060852 2119 32-33 0.5701547339139736 2119 34-35 0.7481874005092131 2119 36-37 0.071719073062944 2119 38-39 0.9271345455610387 2119 40-41 0.23344698372836348 2119 42-43 -0.37948681557358555 2119 44-45 -0.5565253846567373 2119 46-47 -0.027042438074243336 2119 48-49 -0.6395736933813829 2119 50-51 1.206368755092324 2119 52-53 -0.5225323839890343 2119 54-55 -0.7745305688233479 2119 56-57 1.3010191587148938 2119 58-59 1.4761694547979403 2119 60-61 1.1471360993126005 2119 62-63 0.44213591560864174 2119 64-65 0.733554987434033 2119 66-67 0.9194080024388462 2119 68-69 -0.03597846556184692 2119 70-71 -0.5360210520733695 2119 72-73 -1.4845342995600248 2119 74-75 -0.20120143607217145 2119 76 1.0147412785221483 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 3.0 12 23.0 13 159.0 14 579.0 15 1220.0 16 1896.0 17 2481.0 18 2624.0 19 2508.0 20 2170.0 21 1679.0 22 1398.0 23 1121.0 24 1115.0 25 987.0 26 1244.0 27 1294.0 28 1473.0 29 1892.0 30 2091.0 31 2646.0 32 3626.0 33 4551.0 34 6133.0 35 9047.0 36 15229.0 37 17548.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.84005805515239 22.390711175616836 10.597387518142234 28.171843251088536 2 17.178366786953664 27.44388208031175 31.97712625523133 23.400624877503258 3 15.701488407484696 30.40916794447583 28.067606673046104 25.82173697499337 4 11.487600447329282 26.486966346541845 34.12730437990707 27.898128826221797 5 10.767031370695321 35.92584479518545 32.56626353228726 20.740860301831976 6 22.058891348459696 37.253274303634015 17.644346061612247 23.043488286294043 7 21.885700450788015 31.717721387643106 20.24510877710781 26.151469384461073 8 19.530304253086918 38.39999077671582 18.14104707333664 23.928657896860624 9 21.266587500144112 20.671685670475114 19.82314352583096 38.23858330354981 10-11 21.266587500144112 27.329167483311622 26.372251749541718 25.03199326700255 12-13 20.946654830118636 24.94610143306778 25.543309083782006 28.56393465303158 14-15 20.231850306097744 26.613786504029424 22.755571440100532 30.398791749772304 16-17 21.328268213103982 28.53511188996622 24.105053206820617 26.03156669010918 18-19 20.11425343279108 27.528044548462592 24.85271568073602 27.504986338010305 20-21 19.573538397684956 27.955197897091207 25.14209622191222 27.329167483311622 22-23 22.079965873848533 27.457140551321814 24.745495002132884 25.717398572696776 24-25 20.502784278912113 29.280468542836392 24.405386397961653 25.811360780289842 26-27 19.302027969609277 29.563508076138213 25.79925521980239 25.33520873445012 28-29 20.54601842351015 28.14081649123212 27.396612748884557 23.916552336373172 30-31 20.73336638343498 27.71135732155827 26.919884247783532 24.635392047223213 32-33 18.960766454915433 29.365207466248545 26.35611100222512 25.317915076610902 34-35 20.024326412027165 28.883867323057054 26.03214314537049 25.059663119545295 36-37 20.719531457163608 27.7136631426035 26.23044375526015 25.336361644972733 38-39 20.12924126958507 27.206382512653192 27.469246111809266 25.19513010595248 40-41 19.879636141439065 26.778076253501965 27.380472001567956 25.96181560349101 42-43 18.824146557985635 26.832263048064842 28.00073786273447 26.34285253121505 44-45 18.67715046635231 27.267486770351752 28.163298246423096 25.89206451687285 46-47 18.953848991779747 27.76381475033723 27.65889989277932 25.623436365103704 48-49 18.772842039729294 28.112570183428065 26.938907271406663 26.17568050543597 50-51 18.49095541695009 27.918304760367548 26.92391943461268 26.666820388069684 52-53 17.95369911341181 27.832989381694084 27.438693982959983 26.774617521934125 54-55 18.045355499959648 27.647947242814485 26.862238721652815 27.44445853557305 56-57 18.44656836182944 27.62258321131697 26.699101882702887 27.23174654415071 58-59 18.625845948095968 27.296309533417112 26.424132723059362 27.653711795427554 60-61 18.38949929096003 27.046704405271104 26.260419428848124 28.303376874920737 62-63 18.49556705904055 27.027104926386663 26.50022481755191 27.97710319702088 64-65 18.90715611561387 27.684840379538144 25.383054521138614 28.024948983709375 66-67 18.686950205794528 28.670578876373405 24.668249997117726 27.974220920714345 68-69 18.200998420512583 28.72995376828804 25.45107624197286 27.617971569226512 70-71 18.69502057945283 29.234928577193127 24.936878148886866 27.133172694467184 72-73 18.999388957423015 28.460749161257592 25.139213945605682 27.400647935713707 74-75 18.76016002398054 29.167483311620185 24.543159205414067 27.52919745898521 76 18.549177398342113 27.83702456852324 24.91900803578634 28.69478999734831 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 1.0 8 2.0 9 2.0 10 3.5 11 4.5 12 4.0 13 4.5 14 4.5 15 6.0 16 11.5 17 15.0 18 17.0 19 24.0 20 26.0 21 23.0 22 38.0 23 70.0 24 107.0 25 127.0 26 169.5 27 278.5 28 417.0 29 489.0 30 583.5 31 809.0 32 1073.0 33 1206.0 34 1415.0 35 1870.5 36 2274.0 37 2431.0 38 2831.5 39 3675.0 40 4522.0 41 5146.0 42 5366.0 43 5575.0 44 6011.5 45 6280.0 46 6321.0 47 6368.5 48 6187.5 49 5661.5 50 5364.0 51 5038.5 52 4289.5 53 3550.0 54 3234.0 55 2999.5 56 2389.5 57 1802.5 58 1591.0 59 1382.5 60 1014.5 61 742.5 62 630.0 63 563.5 64 414.0 65 294.0 66 222.0 67 187.0 68 149.0 69 101.0 70 70.5 71 50.0 72 48.0 73 38.0 74 25.5 75 21.0 76 18.5 77 16.0 78 15.0 79 14.0 80 11.0 81 7.5 82 6.5 83 6.0 84 5.5 85 5.5 86 5.5 87 5.0 88 5.0 89 4.0 90 4.5 91 5.5 92 5.0 93 5.5 94 4.0 95 1.0 96 0.0 97 0.5 98 3.0 99 6.5 100 8.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.705581239839976 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0011529105226143399 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 86737.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.24363305163887 #Duplication Level Percentage of deduplicated Percentage of total 1 81.20524738438347 52.16920114829888 2 8.154621969384277 10.47765082951912 3 3.797354772715037 7.318675997555829 4 2.19837410046121 5.649261560810266 5 1.3333811890960645 4.283062591512272 6 0.8649929113651456 3.3342172314006713 7 0.5922150637977136 2.663223307239125 8 0.45582614001399785 2.3427141819523385 9 0.3158480340254473 1.8262102678211143 >10 1.0803438436552233 9.85853787887522 >50 0.001794591102417314 0.07724500501516077 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0034587315678430196 0.0 2 0.0 0.0 0.0 0.0034587315678430196 0.0 3 0.0 0.0 0.0 0.0046116420904573595 0.0 4 0.0 0.0 0.0 0.00807037365830038 0.0 5 0.0 0.0 0.0 0.00807037365830038 0.0 6 0.0 0.0 0.0 0.00807037365830038 0.0 7 0.0 0.0 0.0 0.00807037365830038 0.0 8 0.0 0.0 0.0 0.00807037365830038 0.0 9 0.0 0.0 0.0 0.011529105226143399 0.0 10 0.0 0.0 0.0 0.011529105226143399 0.0 11 0.0 0.0 0.0 0.011529105226143399 0.0 12 0.0 0.0 0.0 0.011529105226143399 0.0 13 0.0 0.0 0.0 0.01268201574875774 0.0 14 0.0 0.0 0.0 0.01614074731660076 0.0 15 0.0 0.0 0.0 0.017293657839215097 0.0 16 0.0 0.0 0.0 0.02075238940705812 0.0 17 0.0 0.0 0.0 0.02536403149751548 0.0 18 0.0 0.0 0.0 0.027669852542744157 0.0 19 0.0 0.0 0.0 0.03228149463320152 0.0 20 0.0 0.0 0.0 0.034587315678430194 0.0 21 0.0 0.0 0.0 0.03919895776888756 0.0 22 0.0 0.0 0.0 0.03919895776888756 0.0 23 0.0 0.0 0.0 0.04265768933673058 0.0 24 0.0 0.0 0.0 0.044963510381959254 0.0 25 0.0 0.0 0.0 0.047269331427187936 0.0 26 0.0 0.0 0.0 0.057645526130716995 0.0 27 0.0 0.0 0.0 0.0749391839699321 0.0 28 0.0 0.0 0.0 0.0956915733769902 0.0 29 0.0 0.0 0.0 0.11298523121620531 0.0 30 0.0 0.0 0.0 0.1325847101006491 0.0 31 0.0 0.0 0.0 0.1498783679398642 0.0 32 0.0 0.0 0.0 0.1936889677992091 0.0 33 0.0 0.0 0.0 0.237499567658554 0.0 34 0.0 0.0 0.0 0.2674752412465269 0.0 35 0.0 0.0 0.0 0.33434405155815855 0.0 36 0.0 0.0 0.0 0.4381059985934492 0.0 37 0.0 0.0 0.0 0.6525473557997163 0.0 38 0.0 0.0 0.0 0.8969643865939565 0.0 39 0.0 0.0 0.0 1.1978740329962991 0.0 40 0.0 0.0 0.0 1.522994800373543 0.0 41 0.0 0.0 0.0 1.8769383308161454 0.0 42 0.0 0.0 0.0 2.3046681347060654 0.0 43 0.0 0.0 0.0 2.713951370234156 0.0 44 0.0 0.0 0.0 3.124387516284861 0.0 45 0.0 0.0 0.0 3.5578818727878527 0.0 46 0.0 0.0 0.0 4.0029053345169885 0.0 47 0.0 0.0 0.0 4.43639969101998 0.0 48 0.0 0.0 0.0 4.962126889332119 0.0 49 0.0 0.0 0.0 5.394468335312497 0.0 50 0.0 0.0 0.0 5.829115602338103 0.0 51 0.0 0.0 0.0 6.24301047995665 0.0 52 0.0 0.0 0.0 6.701868867957158 0.0 53 0.0 0.0 0.0 7.151503971776751 0.0 54 0.0 0.0 0.0 7.587304149324971 0.0 55 0.0 0.0 0.0 7.993128653285218 0.0 56 0.0 0.0 0.0 8.39203569410978 0.0 57 0.0 0.0 0.0 8.842823708451988 0.0 58 0.0 0.0 0.0 9.271706422864522 0.0 59 0.0 0.0 0.0 9.697130405709213 0.0 60 0.0 0.0 0.0 10.043003562493515 0.0 61 0.0 0.0 0.0 10.433840229659776 0.0 62 0.0 0.0 0.0 10.885781154524597 0.0 63 0.0 0.0 0.0 11.268547448032558 0.0 64 0.0 0.0 0.0 11.672066130947577 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGTGGCA 20 0.0069327434 52.495678 62 ATGGTTG 20 0.0069327434 52.495678 18 GGACACA 20 0.0069327434 52.495678 24 AAATGAG 20 0.0069327434 52.495678 29 TACTCGG 30 5.851588E-4 46.66282 20 GAAACAC 35 0.0012147804 40.228703 1 TGTGCTA 35 0.0012498654 39.996704 16 CGAGGAG 40 0.0024081313 34.99712 49 ACTTGAA 45 0.0042884653 31.108545 11 TTTCGTT 45 0.0042884653 31.108545 21 TTACTCG 45 0.0042884653 31.108545 19 GTCGCCC 50 0.0071771173 27.997694 37 TTGGTAG 50 0.0071771173 27.997694 67 TGTACTG 50 0.0071771173 27.997694 5 AGGAACA 70 0.0012180132 24.99794 23 GCATGAG 75 0.0018203324 23.331411 55 TTTTCGT 75 0.0018203324 23.331411 20 TGGTCAA 75 0.0018203324 23.331411 17 CATGAGT 80 0.0026487669 21.8732 56 CTTTACA 90 0.005068288 19.55562 1 >>END_MODULE