FastQCFastQC Report
Wed 25 May 2016
SRR1780462_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780462_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86737
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGT36984.2634631126278295No Hit
ATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTCTT33713.8864613717329397RNA PCR Primer, Index 35 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTC10191.1748158225440124No Hit
CTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTC5060.583372724442856No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCG3670.4231181617994627No Hit
TATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTCT3070.35394353044260235No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATATCGTATGCCGT2020.23288792556809668No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCC1710.19714769936705212No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCC1660.1913831467539804No Hit
CTTAATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCG1420.16371329421123626No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGGTTTCACCTCGTATGCCGT1330.1533370995077072No Hit
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC1110.12797306801019173No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCC1080.1245143364423487No Hit
ATACACATCTCCGAGCCCACGAGACTGGGTTTCATATCGTATGCCGTCTT1070.12336142591973438No Hit
TTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTC990.11413814173881964No Hit
ATACACATCTCCGAGCCCACGAGACTGGGTTTCAACTCGTATGCCGTCTT980.11298523121620531No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGGTTTCAACTCGTATGCCGT980.11298523121620531No Hit
CTTATAACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCG950.1095264996483623No Hit
CTTAACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTC870.10030321546744758No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAAG150.002217954370.01
ATAGATA150.002217954370.06
CAAGGAA150.002217954370.04
ACGTATG150.002217954370.040
TGAGACC150.002217954370.019
TCTGCCG308.315199E-658.33333240
GTCTGCC308.315199E-658.33333239
CTGCCGT308.315199E-658.33333241
ATGTCGA252.3786847E-456.00000469
CATGTCG252.3786847E-456.00000468
CTTTATA252.3786847E-456.0000042
TGTCGAA252.3786847E-456.00000470
TGGCCTT200.00693048652.59
ATGAGAC200.00693048652.518
CGACTAA200.00693048652.537
CGGACTA200.00693048652.524
CGTCTGC352.0713927E-550.038
CTTATAA352.0713927E-550.01
CTATACA950.047.8947331
GCTTACA451.6676913E-646.6666682
Produced by FastQC (version 0.11.5)