##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780462_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 86737 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.052987767619356 34.0 34.0 34.0 32.0 34.0 2 33.3866631310744 34.0 34.0 34.0 33.0 34.0 3 33.548335773660604 34.0 34.0 34.0 34.0 34.0 4 33.54596077798402 34.0 34.0 34.0 34.0 34.0 5 33.5519905000173 34.0 34.0 34.0 34.0 34.0 6 37.32703459884478 38.0 38.0 38.0 37.0 38.0 7 36.93560994731199 38.0 37.0 38.0 35.0 38.0 8 36.9707621891465 38.0 37.0 38.0 35.0 38.0 9 36.760771066557524 38.0 37.0 38.0 34.0 38.0 10-11 36.49070754118773 38.0 37.0 38.0 34.0 38.0 12-13 36.33714562412811 38.0 37.0 38.0 34.0 38.0 14-15 36.51694202012982 38.0 37.0 38.0 34.0 38.0 16-17 36.61378073947681 38.0 37.0 38.0 34.0 38.0 18-19 36.66105583545661 38.0 37.0 38.0 34.0 38.0 20-21 36.409513817632615 38.0 37.0 38.0 34.0 38.0 22-23 36.47021455664826 38.0 37.0 38.0 34.0 38.0 24-25 35.72002144413572 38.0 36.5 38.0 31.5 38.0 26-27 35.92213242330263 38.0 37.0 38.0 32.0 38.0 28-29 35.8215294510993 38.0 37.0 38.0 31.5 38.0 30-31 35.484741229233194 38.0 36.0 38.0 29.5 38.0 32-33 35.990033088532 38.0 37.0 38.0 33.0 38.0 34-35 35.85304426023497 38.0 36.5 38.0 32.0 38.0 36-37 35.95325524286061 38.0 37.0 38.0 33.5 38.0 38-39 35.33654034610374 38.0 36.5 38.0 29.0 38.0 40-41 35.26143399010803 37.5 35.5 38.0 29.0 38.0 42-43 35.81594936416985 38.0 36.5 38.0 32.5 38.0 44-45 36.018360100072634 38.0 37.0 38.0 33.0 38.0 46-47 36.25500651394445 38.0 37.0 38.0 34.0 38.0 48-49 36.00973632936348 38.0 37.0 38.0 32.5 38.0 50-51 36.1133368689256 38.0 37.0 38.0 33.5 38.0 52-53 35.992067975604414 38.0 37.0 38.0 33.0 38.0 54-55 36.19177513633167 38.0 37.0 38.0 34.0 38.0 56-57 36.21897229556014 38.0 37.0 38.0 34.0 38.0 58-59 36.210250527456566 38.0 37.0 38.0 34.0 38.0 60-61 35.95719243229533 38.0 37.0 38.0 32.5 38.0 62-63 36.315828308564974 38.0 37.0 38.0 34.0 38.0 64-65 36.30365357344617 38.0 37.0 38.0 34.0 38.0 66-67 35.50234617291352 38.0 36.0 38.0 31.0 38.0 68-69 34.323425988908994 37.5 34.5 38.0 24.0 38.0 70-71 33.66157464519179 37.0 34.0 38.0 22.0 38.0 72-73 32.884789651475145 37.0 33.0 38.0 16.0 38.0 74-75 33.26166457221255 37.5 34.0 38.0 19.0 38.0 76 32.85339589794436 37.0 33.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.25505970144959633 1101 2 0.33676125163742654 1101 3 0.12598394741922192 1101 4 0.29538784530001294 1101 5 0.4049682825577179 1101 6 -1.11786956569307 1101 7 0.172055293929958 1101 8 0.17141672716090994 1101 9 0.16830255531985472 1101 10-11 -0.17748952233762338 1101 12-13 0.47839446919333994 1101 14-15 0.7190001202758154 1101 16-17 -0.2419422444661592 1101 18-19 -0.6034424702854011 1101 20-21 -0.11802356166547412 1101 22-23 0.4179946657870772 1101 24-25 -1.4709297263470944 1101 26-27 -0.02171235859422893 1101 28-29 0.5703296038926524 1101 30-31 0.8928585066127894 1101 32-33 0.039043448032508365 1101 34-35 -0.13231025330932056 1101 36-37 0.23544347606149785 1101 38-39 0.0936916201792215 1101 40-41 1.2028173307054075 1101 42-43 0.468147961981515 1101 44-45 -0.25263712706320973 1101 46-47 0.36525850729114495 1101 48-49 -2.5475971571523957 1101 50-51 -1.90398321423978 1101 52-53 -3.0604801761804836 1101 54-55 -1.4111634026268476 1101 56-57 -1.2173040633333585 1101 58-59 -1.4938033277187799 1101 60-61 -2.223202903764019 1101 62-63 -0.7599633682584681 1101 64-65 -1.51612211161045 1101 66-67 -1.8322206376138936 1101 68-69 -2.062139723382984 1101 70-71 -1.3767197860456832 1101 72-73 -2.0337563585755767 1101 74-75 -1.3843267826977836 1101 76 -1.5687171828524633 1102 1 -0.31966040602912926 1102 2 0.03763451630379677 1102 3 0.16928891831040005 1102 4 0.2007617817084295 1102 5 0.007274596933001476 1102 6 0.010836211288570041 1102 7 -0.26168854843457723 1102 8 0.21494561206910134 1102 9 0.6005461737703754 1102 10-11 0.48244106431710776 1102 12-13 0.35933938634514817 1102 14-15 -0.093382324865253 1102 16-17 -0.08167578678591525 1102 18-19 -0.5049650856011212 1102 20-21 0.17790120322480618 1102 22-23 0.38660192060122256 1102 24-25 -4.662868821734467 1102 26-27 -1.352354991823077 1102 28-29 -0.013895653608472003 1102 30-31 1.4557895410955481 1102 32-33 0.3443502102358238 1102 34-35 0.5613417843082331 1102 36-37 -2.739802829357224 1102 38-39 -0.2490535656694206 1102 40-41 1.05041025502247 1102 42-43 0.7585868334548707 1102 44-45 0.480602908763025 1102 46-47 0.8254242036637436 1102 48-49 -2.963114398531708 1102 50-51 -0.681871705059315 1102 52-53 -0.07746405437126214 1102 54-55 0.27473001430105626 1102 56-57 0.1889543334422754 1102 58-59 -0.2240093286144358 1102 60-61 -6.205860763146013 1102 62-63 -2.2202074345370164 1102 64-65 -0.25163487470942414 1102 66-67 0.9219105760000801 1102 68-69 -0.8866359168446962 1102 70-71 -1.7205845419793988 1102 72-73 -5.499217621450633 1102 74-75 -2.442029424883188 1102 76 -1.4169034793146231 1103 1 0.64703718209703 1103 2 0.3564656851349639 1103 3 0.12049485523062486 1103 4 0.3152330247511017 1103 5 0.33037292717418865 1103 6 0.28578982909932904 1103 7 0.7985155331980778 1103 8 0.610121864388212 1103 9 0.3690062850876288 1103 10-11 0.11068781756385704 1103 12-13 -0.9555956785899227 1103 14-15 -0.36657341948491506 1103 16-17 0.2553835824163144 1103 18-19 0.3442000349925678 1103 20-21 -0.3256238431573806 1103 22-23 0.32482084453443605 1103 24-25 -3.484019100027602 1103 26-27 -0.6570395929362434 1103 28-29 -0.5455746888589346 1103 30-31 0.783850765585349 1103 32-33 0.7468548484547526 1103 34-35 0.19274252642325962 1103 36-37 -0.8943946660919195 1103 38-39 -0.247476571235282 1103 40-41 -0.32329104367882877 1103 42-43 -0.1405782871388226 1103 44-45 1.0214656174828747 1103 46-47 0.8782071350181013 1103 48-49 -2.2518899087357838 1103 50-51 -0.19490510023556595 1103 52-53 0.2571834409905236 1103 54-55 -0.1709100599104474 1103 56-57 0.39057771006565645 1103 58-59 -0.592747733067128 1103 60-61 -3.8685231008083534 1103 62-63 -1.2369514048524124 1103 64-65 0.45467310302712605 1103 66-67 -0.3176534314210713 1103 68-69 0.027093211170146958 1103 70-71 0.3101204672970397 1103 72-73 -4.706592389539686 1103 74-75 -2.5621592950130534 1103 76 -3.0376827000938427 1104 1 0.3652839744848677 1104 2 0.245555498275408 1104 3 -0.07631906020897361 1104 4 -0.04484619681094415 1104 5 -0.04644615488901849 1104 6 0.053567319842464656 1104 7 0.45961946844305857 1104 8 0.678390184374436 1104 9 0.9328054756499284 1104 10-11 -0.7265635386748386 1104 12-13 0.19618250103636115 1104 14-15 0.48715085503501854 1104 16-17 0.1568646811849348 1104 18-19 0.7144058388330947 1104 20-21 -0.23318433570883457 1104 22-23 0.14452290257283806 1104 24-25 0.7085926282079953 1104 26-27 0.8011451999682606 1104 28-29 1.0658607714011197 1104 30-31 0.6341650685217104 1104 32-33 0.22611085465469216 1104 34-35 -0.5723655421206146 1104 36-37 0.9122013900520614 1104 38-39 0.9995655367471556 1104 40-41 1.455732464458606 1104 42-43 0.2668530398223439 1104 44-45 0.5643485934580781 1104 46-47 0.6415154963373126 1104 48-49 -0.2852662972658848 1104 50-51 0.1539453260108843 1104 52-53 0.3953303453218737 1104 54-55 -0.17785916866787232 1104 56-57 -0.2584277148891374 1104 58-59 -1.5266752281157778 1104 60-61 0.37439431932426004 1104 62-63 -0.270543069366326 1104 64-65 0.35887529023112563 1104 66-67 -0.25071121149512265 1104 68-69 1.1434369638610917 1104 70-71 0.7781304561026872 1104 72-73 0.2898598622470985 1104 74-75 -0.15390130827405102 1104 76 -1.0716790834573153 1105 1 0.6552004474031534 1105 2 0.32381262391047017 1105 3 0.1980458756387833 1105 4 -0.019460852799916495 1105 5 -0.19207605241764725 1105 6 0.008238808691167776 1105 7 0.7413726760552208 1105 8 -0.4184495641832129 1105 9 0.6383940401896666 1105 10-11 0.49027965429855414 1105 12-13 -0.28824873981440646 1105 14-15 0.1987327029640582 1105 16-17 0.1288529701714154 1105 18-19 0.5686898309109409 1105 20-21 0.4845802384752673 1105 22-23 0.2635963547385103 1105 24-25 0.7261849816050514 1105 26-27 0.24500122339028252 1105 28-29 0.4483028621614764 1105 30-31 1.3858915819118778 1105 32-33 0.6529772974343402 1105 34-35 -1.1072574735767446 1105 36-37 -0.11888446201027847 1105 38-39 -0.035231673276094 1105 40-41 -0.07227063551556512 1105 42-43 0.17370742714689413 1105 44-45 0.7704452093196181 1105 46-47 0.6516765227732009 1105 48-49 -0.3743388883276211 1105 50-51 0.8601969405807637 1105 52-53 0.7326936450721533 1105 54-55 0.6943960625385301 1105 56-57 0.3150675059840182 1105 58-59 0.19092573632062937 1105 60-61 0.7233136338855246 1105 62-63 -0.3532779354646607 1105 64-65 0.6689588173128413 1105 66-67 0.6231628951095516 1105 68-69 -0.050457809238011464 1105 70-71 1.1182837326031603 1105 72-73 0.8342239369909237 1105 74-75 0.38273866417061697 1105 76 0.7296642386816643 1106 1 -0.8503636650342727 1106 2 0.2318026123586847 1106 3 0.01242785484556208 1106 4 -0.2438351308130109 1106 5 -0.10070138934487005 1106 6 0.07104204319252005 1106 7 -0.595650812585518 1106 8 0.16786156404165808 1106 9 -0.8500541692827994 1106 10-11 0.3718064159466081 1106 12-13 1.2059946179060361 1106 14-15 -0.5390512785530674 1106 16-17 -0.1597203836984349 1106 18-19 0.27779649244347127 1106 20-21 -0.04881406264140509 1106 22-23 0.4798266204296979 1106 24-25 -0.45900947353549526 1106 26-27 0.6147547851923605 1106 28-29 0.5599894235785001 1106 30-31 0.5095631260012112 1106 32-33 0.19748057044164824 1106 34-35 0.8449181136392738 1106 36-37 0.9389707555484392 1106 38-39 0.10253305525682777 1106 40-41 0.9872884711459733 1106 42-43 1.0050705384291376 1106 44-45 0.8147366995005925 1106 46-47 0.48099881772892417 1106 48-49 0.6708478656192369 1106 50-51 -0.14988199665450708 1106 52-53 -0.0724469017126026 1106 54-55 -0.2515993167452564 1106 56-57 0.3001464432192904 1106 58-59 0.2340524209567505 1106 60-61 0.7770294598385519 1106 62-63 0.5589092035418801 1106 64-65 -1.2179299004384063 1106 66-67 -0.09091103634983 1106 68-69 1.3787585943045286 1106 70-71 -0.21976979069295055 1106 72-73 -0.2618677072139235 1106 74-75 -1.2994908314011937 1106 76 -0.4739360693660828 1107 1 0.5923695319044242 1107 2 -0.20922565164071472 1107 3 0.21817537322095149 1107 4 0.24161610810492107 1107 5 0.16030489996330033 1107 6 0.3200001397296077 1107 7 0.5943355479509691 1107 8 -0.2376283200228997 1107 9 -1.0740065827100977 1107 10-11 0.22600623408709453 1107 12-13 -0.065980073311664 1107 14-15 0.03135297999053677 1107 16-17 0.584397597660967 1107 18-19 0.8005478753335211 1107 20-21 0.6354039414504342 1107 22-23 0.1274927566727797 1107 24-25 0.24158535862332542 1107 26-27 -0.3760954080333718 1107 28-29 0.5429590378847777 1107 30-31 0.2878176535453534 1107 32-33 0.9565261868696311 1107 34-35 0.2556554024170339 1107 36-37 0.8843939577913744 1107 38-39 0.07772628098994971 1107 40-41 0.4545632305609857 1107 42-43 0.515925278142717 1107 44-45 0.35647914096456645 1107 46-47 0.5842230353541424 1107 48-49 0.8061627099020328 1107 50-51 1.102931142695347 1107 52-53 0.5077038901340316 1107 54-55 0.25910387337371077 1107 56-57 0.48157025166060663 1107 58-59 0.4912699703998058 1107 60-61 0.8813334683253302 1107 62-63 0.2218060740427532 1107 64-65 0.16391824686779444 1107 66-67 -0.49609782122518453 1107 68-69 0.3301585336846955 1107 70-71 -0.8664224390221165 1107 72-73 0.4115618601119877 1107 74-75 -0.9020330758506887 1107 76 0.07889790805302965 1108 1 -0.49815002664473695 1108 2 0.4886366637626338 1108 3 0.09677639146716643 1108 4 0.37628075092818847 1108 5 0.09206343014702867 1108 6 -0.06690179873289992 1108 7 0.037130349203216895 1108 8 -0.2745318396130756 1108 9 -0.035316388863840587 1108 10-11 0.39949546660773194 1108 12-13 1.0410859862019848 1108 14-15 0.4643843179407625 1108 16-17 0.058356425096690145 1108 18-19 0.20953026157138765 1108 20-21 1.0367415754510034 1108 22-23 0.011619816091560153 1108 24-25 0.9378112068983171 1108 26-27 -0.5422798307636612 1108 28-29 -0.9142714589055316 1108 30-31 0.01307300566248415 1108 32-33 0.7299980269860029 1108 34-35 0.6425336239646384 1108 36-37 1.1422837848160086 1108 38-39 1.155849798682766 1108 40-41 1.074515480505653 1108 42-43 -0.29488489167039234 1108 44-45 0.6723172967372619 1108 46-47 0.8168701592829279 1108 48-49 0.9750745778462502 1108 50-51 0.42118842378821597 1108 52-53 0.04499478600096296 1108 54-55 -0.674196256278762 1108 56-57 0.24974531411514533 1108 58-59 0.3391259612925026 1108 60-61 0.7588672254008699 1108 62-63 0.8000323650334948 1108 64-65 0.643569133880419 1108 66-67 0.8812699174460406 1108 68-69 1.413578829039345 1108 70-71 -0.32534474240888756 1108 72-73 0.2902419347412035 1108 74-75 -0.2074911285338672 1108 76 1.0068617318521618 1109 1 -1.0311286449032266 1109 2 0.07732641436389542 1109 3 -0.004959845478111902 1109 4 0.02651301791991756 1109 5 0.1547260994054227 1109 6 -0.18399071910321396 1109 7 -0.32173038943876264 1109 8 0.11696158772524967 1109 9 0.2872711642610497 1109 10-11 0.25264836747420105 1109 12-13 0.47632303031623735 1109 14-15 0.6297633579038617 1109 16-17 0.6857738138226779 1109 18-19 0.7053049790622481 1109 20-21 0.7802595143697246 1109 22-23 0.18340678937262567 1109 24-25 -0.16085284607832762 1109 26-27 0.2888488879791282 1109 28-29 -0.54376442500444 1109 30-31 0.5768146456980574 1109 32-33 0.5363433635260506 1109 34-35 0.03584729971333189 1109 36-37 0.6709712292201857 1109 38-39 1.0602085111658823 1109 40-41 0.43600363653635554 1109 42-43 1.0363920825990363 1109 44-45 0.6349486676066931 1109 46-47 0.4893078095975554 1109 48-49 0.7232872751084614 1109 50-51 0.09801608497492253 1109 52-53 -0.28076378811032754 1109 54-55 -0.3199067297080731 1109 56-57 0.22745756661078076 1109 58-59 0.19331665084543914 1109 60-61 0.8864081603990641 1109 62-63 -0.021871565385247038 1109 64-65 0.7678402108744535 1109 66-67 0.7038904162435173 1109 68-69 -0.23627456271765368 1109 70-71 -0.1378344468862096 1109 72-73 -0.2803606416103861 1109 74-75 -0.16490884671657824 1109 76 0.41192877629587343 1110 1 -0.5817816181503446 1110 2 -0.2582529295836551 1110 3 0.04850969753118051 1110 4 0.16656264750929495 1110 5 0.4373611770195822 1110 6 0.10261110306346666 1110 7 0.4649348281888024 1110 8 0.7543395514630404 1110 9 0.8585548317790312 1110 10-11 -0.45208274520670955 1110 12-13 0.415399992405753 1110 14-15 0.6527215712386507 1110 16-17 0.5433242132140919 1110 18-19 -0.09933737997342007 1110 20-21 0.9133990387226376 1110 22-23 -0.6406708066715083 1110 24-25 -1.32434068320633 1110 26-27 0.8609566964886142 1110 28-29 -0.1111900259028431 1110 30-31 -0.36314985284383994 1110 32-33 0.836774452660066 1110 34-35 0.9185928665593153 1110 36-37 1.277837863283473 1110 38-39 0.49964773303188537 1110 40-41 -1.6172304376182254 1110 42-43 -0.14769022282218458 1110 44-45 0.87335182651195 1110 46-47 0.7719610002005339 1110 48-49 1.2379678525505469 1110 50-51 -0.3564388046264142 1110 52-53 0.6323844304772237 1110 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2116 38-39 -4.471819359197923 2116 40-41 -1.058642133267206 2116 42-43 -1.0041635517531375 2116 44-45 -0.10063746103666915 2116 46-47 -0.5557776453347145 2116 48-49 0.20722458451914605 2116 50-51 -1.4130735540577675 2116 52-53 0.11055597644537585 2116 54-55 -0.36016983161574245 2116 56-57 0.13325325485983797 2116 58-59 -1.9728151837762269 2116 60-61 -1.3763923502030266 2116 62-63 0.33553216484664716 2116 64-65 -1.7443929640776759 2116 66-67 -0.7775116119814243 2116 68-69 -1.6833616487399183 2116 70-71 0.13600251503138594 2116 72-73 -1.035812382621664 2116 74-75 -0.9926072369016694 2116 76 -1.0602008599001387 2117 1 0.39135587027099206 2117 2 0.3758044249977175 2117 3 0.079928060827271 2117 4 -0.176808682761596 2117 5 0.1311928184494775 2117 6 0.27673537762441924 2117 7 0.5031586131374794 2117 8 0.2448781249710521 2117 9 -0.5906408051895298 2117 10-11 -0.30135210757650555 2117 12-13 -1.2637054727259098 2117 14-15 0.2280349041938976 2117 16-17 -0.31753326991413644 2117 18-19 -0.4266403535645935 2117 20-21 0.19540815042607562 2117 22-23 0.30752292099820977 2117 24-25 -1.1639406756447528 2117 26-27 -1.3833295849155718 2117 28-29 -1.017974653211482 2117 30-31 -0.25960347200489053 2117 32-33 -1.5559078286685022 2117 34-35 0.36234416620580845 2117 36-37 -1.0021390738987108 2117 38-39 0.4101564383004117 2117 40-41 0.7171331609375144 2117 42-43 0.5782970359161652 2117 44-45 0.0076076725581799565 2117 46-47 -0.5396611476661519 2117 48-49 -0.48473011905571894 2117 50-51 -0.2501229952538395 2117 52-53 0.5955311818335858 2117 54-55 -0.3315820142817145 2117 56-57 0.5996232496788778 2117 58-59 -0.5826238581896703 2117 60-61 0.04913129719539455 2117 62-63 -0.25844681524365143 2117 64-65 -1.0121747714938607 2117 66-67 -0.502235910700982 2117 68-69 0.43821521455665646 2117 70-71 -0.031203835349785436 2117 72-73 0.9563699132853714 2117 74-75 0.71301225832444 2117 76 0.7172767509354756 2118 1 0.03056132346089413 2118 2 0.4023425724752627 2118 3 -0.41881159905840804 2118 4 0.2305474432014094 2118 5 0.3879471763860636 2118 6 0.3483084951023159 2118 7 0.7346529149800602 2118 8 0.4494615026825528 2118 9 -1.257325223125413 2118 10-11 -0.9424480709378855 2118 12-13 -1.0607640874168638 2118 14-15 0.6391010454229971 2118 16-17 -2.4901199848642577 2118 18-19 -0.6394070663845639 2118 20-21 -0.31160360091414674 2118 22-23 0.7078140418133501 2118 24-25 0.866769020098495 2118 26-27 -2.19265432663461 2118 28-29 -0.15020385759959254 2118 30-31 -0.5505112719125762 2118 32-33 0.17897696559428056 2118 34-35 -0.06572769093197195 2118 36-37 -2.5814694960238924 2118 38-39 0.2504660204355318 2118 40-41 -0.12137466737220848 2118 42-43 0.3609149931001028 2118 44-45 -1.7631701880588508 2118 46-47 0.2921278252454158 2118 48-49 0.5295685282975668 2118 50-51 -0.5295989777865842 2118 52-53 -1.0930405510453198 2118 54-55 -0.6525427129716732 2118 56-57 0.5609609853269717 2118 58-59 -0.36975951338901325 2118 60-61 -0.5110898836190358 2118 62-63 -0.8815341159856516 2118 64-65 0.20985810385673176 2118 66-67 0.7997930593703728 2118 68-69 -0.8194888362829786 2118 70-71 0.15365788226301902 2118 72-73 0.9981031472116015 2118 74-75 0.44533199418555114 2118 76 0.25198048225226444 2119 1 0.72124868487996 2119 2 -0.6941836655143874 2119 3 -0.08980199078050788 2119 4 0.12348905443570146 2119 5 -0.17908903943063592 2119 6 -0.7118910614386991 2119 7 -0.64350673025276 2119 8 -0.0032362061127173547 2119 9 -0.23581746259326053 2119 10-11 0.13016833704438113 2119 12-13 0.05933938634515101 2119 14-15 -0.20474596122888755 2119 16-17 -0.8634939686040894 2119 18-19 -0.8367832674193068 2119 20-21 -1.2334624331388326 2119 22-23 -0.08612535212604655 2119 24-25 0.8825857237200836 2119 26-27 0.4180995536314711 2119 28-29 0.10883161911880279 2119 30-31 1.2830622683682762 2119 32-33 0.1307138465994555 2119 34-35 0.20679632976277418 2119 36-37 -0.11707555662994906 2119 38-39 -1.8808717474875962 2119 40-41 -1.0154988358866177 2119 42-43 0.03358683345487634 2119 44-45 0.0351483633084797 2119 46-47 0.2731514763910141 2119 48-49 -0.28811439853171095 2119 50-51 0.9181282949406935 2119 52-53 0.20435412744691916 2119 54-55 0.2951845597556044 2119 56-57 -1.363318393830454 2119 58-59 -0.23764569225080123 2119 60-61 -0.030860763146016268 2119 62-63 -0.4883892527188394 2119 64-65 -0.21527123834578532 2119 66-67 0.7400923941819002 2119 68-69 0.8883640831553024 2119 70-71 -0.5546754510703096 2119 72-73 1.0939641967311857 2119 74-75 -0.3965748794286341 2119 76 0.560369247958107 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 2.0 22 5.0 23 15.0 24 48.0 25 108.0 26 214.0 27 410.0 28 697.0 29 1086.0 30 1674.0 31 2679.0 32 3980.0 33 6048.0 34 8754.0 35 11616.0 36 17164.0 37 32236.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.52736433125425 23.08818612587477 11.103681243298707 31.28076829957227 2 16.99159528229014 18.19984550998997 43.335600724027806 21.472958483692082 3 15.195360688056999 27.168336465406917 35.13840690823985 22.497895938296228 4 11.301981853188375 22.93139029479922 36.80090388184973 28.965723970162678 5 10.647128676343428 38.62134959705777 37.41655810092579 13.314963625673013 6 22.007908966185134 39.98408983478792 17.940440642401743 20.0675605566252 7 22.1819984550999 34.925118461556195 20.783517991168704 22.109365092175196 8 19.67211224736848 36.765163655648685 23.042069704970196 20.520654392012634 9 19.659430231619723 18.361252983155975 19.306639611699737 42.67267717352456 10-11 20.33907098470088 25.62401282036501 30.365357344616484 23.671558850317627 12-13 22.500201759341458 20.44225647647486 27.899281736744413 29.15826002743927 14-15 22.67832643508537 23.46461141150835 21.434912436445806 32.42214971696047 16-17 24.031266933373303 27.77534385556337 21.5467447571394 26.64664445392393 18-19 22.465037988401722 26.976953318652942 22.458120525266033 28.099888167679303 20-21 21.101144840148955 24.923043222615494 22.228114876004472 31.74769706123108 22-23 22.814369876753865 29.69205759940971 22.997106194588238 24.496466329248186 24-25 23.50611619032247 30.892237453451237 22.867403760794126 22.734242595432168 26-27 24.80948153613798 28.545488084669753 25.60614270726449 21.03888767192778 28-29 25.87419440377232 23.8865766627852 27.839330389568467 22.399898543874013 30-31 28.016878610051073 22.720984124422102 29.574460726103048 19.687676539423776 32-33 23.21500628336235 23.158513667754246 31.71195683503003 21.914523213853375 34-35 19.235735614558955 25.350773026505415 34.91647163263659 20.497019726299044 36-37 18.45002709339728 27.097432468266135 28.40598591143341 26.046554526903165 38-39 20.738554480786746 24.50799543447433 28.012266967960613 26.741183116778306 40-41 19.581608771343255 24.94206624623863 30.76368793017974 24.712637052238374 42-43 24.27798978521277 24.146557985634733 31.14818358947162 20.427268639680875 44-45 19.640983663257895 26.096706134636893 29.012993301589862 25.24931690051535 46-47 24.42902106367525 22.617222177386815 27.39373047257802 25.560026286359914 48-49 22.201597933984342 22.243679168059767 28.299918143352894 27.254804754602997 50-51 18.26498495451768 22.372228691331266 32.13276917578426 27.23001717836679 52-53 19.587949779217634 22.43218003850721 35.649146269758006 22.33072391251715 54-55 17.87587765313534 22.561306017040017 32.866596723428295 26.696219606396348 56-57 22.96251887890981 22.467920264708255 29.50067445265573 25.06888640372621 58-59 18.758430658196616 28.122369922870288 31.38280088082364 21.73639853810946 60-61 20.360399829369243 34.110587177329165 24.795070154605302 20.73394283869629 62-63 16.482008831294603 38.59540911029895 23.765521057910696 21.15706100049575 64-65 16.85324601957642 39.784636314375646 22.596469787979757 20.765647878068183 66-67 16.582312046762052 38.96779920910338 22.520954148748515 21.928934595386053 68-69 16.70855574898832 37.587765313534014 22.82128733988955 22.88239159758811 70-71 17.332856796983986 35.539619770109645 23.174654415070847 23.952869017835525 72-73 17.648754280180317 33.10870793317731 23.53436249812652 25.70817528851586 74-75 18.16468173905023 32.49939472197563 23.47325824042796 25.86266529854618 76 18.09838938399991 32.29187082790504 23.82604886034795 25.7836909277471 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 0.5 11 0.0 12 0.0 13 0.5 14 1.5 15 2.0 16 4.5 17 7.0 18 10.0 19 11.0 20 15.0 21 21.0 22 30.5 23 49.0 24 65.0 25 72.0 26 129.0 27 249.0 28 331.0 29 350.0 30 450.0 31 680.5 32 974.5 33 1138.0 34 1309.5 35 1626.0 36 1984.0 37 2197.0 38 2541.5 39 3523.0 40 5394.5 41 7643.0 42 8657.0 43 8627.0 44 8250.5 45 7344.0 46 6784.0 47 6425.0 48 5674.5 49 4930.0 50 4577.0 51 4179.0 52 3411.5 53 2734.5 54 2427.0 55 2321.5 56 1849.0 57 1283.0 58 1084.0 59 879.5 60 570.0 61 411.0 62 357.0 63 284.5 64 178.0 65 128.5 66 103.5 67 94.0 68 68.5 69 29.5 70 14.0 71 12.0 72 8.0 73 3.5 74 2.0 75 1.0 76 0.5 77 0.5 78 0.5 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 86737.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.690950805308 #Duplication Level Percentage of deduplicated Percentage of total 1 60.553040499204705 22.823016705673474 2 15.906032056772299 11.990269435189136 3 8.164076838370244 9.23135455457302 4 4.27321668909825 6.442464000368932 5 2.9609690444145356 5.5800869294534055 6 1.917900403768506 4.337249386075147 7 1.2174232228068027 3.212008716003551 8 0.9788327419552184 2.95145093789271 9 0.7769484889269546 2.6355534546963812 >10 3.17508870671724 17.847054890069984 >50 0.033647375504710635 0.949998270634216 >100 0.030588523186100575 2.0913796880224123 >500 0.0030588523186100575 0.583372724442856 >1k 0.009176556955830173 9.324740306904781 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGT 3698 4.2634631126278295 No Hit ATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTCTT 3371 3.8864613717329397 RNA PCR Primer, Index 35 (95% over 21bp) CTTTACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTC 1019 1.1748158225440124 No Hit CTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTC 506 0.583372724442856 No Hit TCTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCG 367 0.4231181617994627 No Hit TATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTCT 307 0.35394353044260235 No Hit CTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATATCGTATGCCGT 202 0.23288792556809668 No Hit CTCTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCC 171 0.19714769936705212 No Hit TCTTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCC 166 0.1913831467539804 No Hit CTTAATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCG 142 0.16371329421123626 No Hit CTTATACACATCTCCGAGCCCACGAGACTGGGTTTCACCTCGTATGCCGT 133 0.1533370995077072 No Hit ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC 111 0.12797306801019173 No Hit TCTTAATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCC 108 0.1245143364423487 No Hit ATACACATCTCCGAGCCCACGAGACTGGGTTTCATATCGTATGCCGTCTT 107 0.12336142591973438 No Hit TTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTC 99 0.11413814173881964 No Hit ATACACATCTCCGAGCCCACGAGACTGGGTTTCAACTCGTATGCCGTCTT 98 0.11298523121620531 No Hit CTTATACACATCTCCGAGCCCACGAGACTGGGTTTCAACTCGTATGCCGT 98 0.11298523121620531 No Hit CTTATAACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCG 95 0.1095264996483623 No Hit CTTAACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTC 87 0.10030321546744758 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0034587315678430196 0.0 2 0.0 0.0 0.0 0.0034587315678430196 0.0 3 0.0 0.0 0.0 0.0046116420904573595 0.0 4 0.0 0.0 0.0 0.00807037365830038 0.0 5 0.0 0.0 0.0 0.00807037365830038 0.0 6 0.0 0.0 0.0 0.00807037365830038 0.0 7 0.0 0.0 0.0 0.00807037365830038 0.0 8 0.0 0.0 0.0 0.00807037365830038 0.0 9 0.0 0.0 0.0 0.011529105226143399 0.0 10 0.0 0.0 0.0 0.011529105226143399 0.0 11 0.0 0.0 0.0 0.011529105226143399 0.0 12 0.0 0.0 0.0 0.011529105226143399 0.0 13 0.0 0.0 0.0 0.011529105226143399 0.0 14 0.0 0.0 0.0 0.01498783679398642 0.0 15 0.0 0.0 0.0 0.01498783679398642 0.0 16 0.0 0.0 0.0 0.018446568361829438 0.0 17 0.0 0.0 0.0 0.023058210452286797 0.0 18 0.0 0.0 0.0 0.026516942020129816 0.0 19 0.0 0.0 0.0 0.03228149463320152 0.0 20 0.0 0.0 0.0 0.036893136723658876 0.0 21 0.0 0.0 0.0 0.04265768933673058 0.0 22 0.0 0.0 0.0 0.04265768933673058 0.0 23 0.0 0.0 0.0 0.047269331427187936 0.0 24 0.0 0.0 0.0 0.04957515247241662 0.0 25 0.0 0.0 0.0 0.0518809735176453 0.0 26 0.0 0.0 0.0 0.06110425769856002 0.0 27 0.0 0.0 0.0 0.07724500501516077 0.0 28 0.0 0.0 0.0 0.10145612599006192 0.0 29 0.0 0.0 0.0 0.12220851539712003 0.0 30 0.0 0.0 0.0 0.14296090480417814 0.0 31 0.0 0.0 0.0 0.16025456264339324 0.0 32 0.0 0.0 0.0 0.20406516250273815 0.0 33 0.0 0.0 0.0 0.2547932254977691 0.0 34 0.0 0.0 0.0 0.28707472013097063 0.0 35 0.0 0.0 0.0 0.36547263566874577 0.0 36 0.0 0.0 0.0 0.474999135317108 0.0 37 0.0 0.0 0.0 0.695205045136447 0.0 38 0.0 0.0 0.0 0.9430808074985301 0.0 39 0.0 0.0 0.0 1.2601312012174735 0.0 40 0.0 0.0 0.0 1.594475252775632 0.0 41 0.0 0.0 0.0 1.9553362463539206 0.0 42 0.0 0.0 0.0 2.3957480659925983 0.0 43 0.0 0.0 0.0 2.8211720488372896 0.0 44 0.0 0.0 0.0 3.2500547632498242 0.0 45 0.0 0.0 0.0 3.6950782249789595 0.0 46 0.0 0.0 0.0 4.186218107612668 0.0 47 0.0 0.0 0.0 4.6589114218845475 0.0 48 0.0 0.0 0.0 5.223837577965574 0.0 49 0.0 0.0 0.0 5.681543055443467 0.0 50 0.0 0.0 0.0 6.1507776381475034 0.0 51 0.0 0.0 0.0 6.596954010399253 0.0 52 0.0 0.0 0.0 7.086940982510347 0.0 53 0.0 0.0 0.0 7.568857580963141 0.0 54 0.0 0.0 0.0 8.039245074189791 0.0 55 0.0 0.0 0.0 8.476198162260626 0.0 56 0.0 0.0 0.0 8.900469234582705 0.0 57 0.0 0.0 0.0 9.378927101467655 0.0 58 0.0 0.0 0.0 9.82395056319679 0.0 59 0.0 0.0 0.0 10.286267682765141 0.0 60 0.0 0.0 0.0 10.636752481639899 0.0 61 0.0 0.0 0.0 11.04027116455492 0.0 62 0.0 0.0 0.0 11.517576120917255 0.0 63 0.0 0.0 0.0 11.93031808801319 0.0 64 0.0 0.0 0.0 12.356894981380496 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACAAG 15 0.0022179543 70.0 1 ATAGATA 15 0.0022179543 70.0 6 CAAGGAA 15 0.0022179543 70.0 4 ACGTATG 15 0.0022179543 70.0 40 TGAGACC 15 0.0022179543 70.0 19 TCTGCCG 30 8.315199E-6 58.333332 40 GTCTGCC 30 8.315199E-6 58.333332 39 CTGCCGT 30 8.315199E-6 58.333332 41 ATGTCGA 25 2.3786847E-4 56.000004 69 CATGTCG 25 2.3786847E-4 56.000004 68 CTTTATA 25 2.3786847E-4 56.000004 2 TGTCGAA 25 2.3786847E-4 56.000004 70 TGGCCTT 20 0.006930486 52.5 9 ATGAGAC 20 0.006930486 52.5 18 CGACTAA 20 0.006930486 52.5 37 CGGACTA 20 0.006930486 52.5 24 CGTCTGC 35 2.0713927E-5 50.0 38 CTTATAA 35 2.0713927E-5 50.0 1 CTATACA 95 0.0 47.894733 1 GCTTACA 45 1.6676913E-6 46.666668 2 >>END_MODULE