FastQCFastQC Report
Wed 25 May 2016
SRR1780461_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780461_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences479828
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCGAT454.00863E-529.3928716
TTGGCCG300.0057815129.2917885
TGCGATT508.234527E-526.44528617
AACATTG1006.184564E-1126.37085342
GAACATT1051.1277734E-1025.11509941
TACTGAC651.8010762E-523.70816434
ACATGTT651.8245033E-523.6686229
GACTATC752.0479893E-623.4823738
CGTTGAT1105.113179E-922.04465116
ATTGCGA703.1602667E-522.04465115
TAACGGC703.193591E-522.01472536
TTAACGG703.193591E-522.01472535
TCTCGTT500.002568187722.01472340
GACAGTT703.2350225E-521.97800330
CTCGTTG500.002594778821.97571241
TTAGCGC500.002594778821.97571231
TACATGC602.8948736E-421.971132
AAGAACG500.002599494821.9688423
TATACCG500.002599494821.9688426
TGTACAC500.002599494821.9688423
Produced by FastQC (version 0.11.5)