FastQCFastQC Report
Wed 25 May 2016
SRR1780461_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780461_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences479828
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGT284055.919829605608676No Hit
ATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGTCTT230294.799428128412681RNA PCR Primer, Index 45 (95% over 23bp)
CTTTACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGTC62241.2971314721108396RNA PCR Primer, Index 45 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGTC36500.7606892469801679No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCG33100.6898305226039331No Hit
TATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGTCT21030.4382820510682995RNA PCR Primer, Index 45 (95% over 22bp)
CTCTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCC17280.3601290462415699No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCC11840.24675508723959416No Hit
CTTAATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCG9410.1961119401118734No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCC7630.1590153138207858No Hit
CTTATAACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCG6750.14067540868811323No Hit
TTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGTC6020.1254616237485099No Hit
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC5730.11941779137524279No Hit
CTTAACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGTC5120.10670490259009478RNA PCR Primer, Index 45 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAA800.044.047781
CCGACGT207.8542746E-443.99728813
CCGTCTT25800.041.78472544
GCCGTCT28350.038.0263143
CGGTATG301.299639E-436.66823243
CTATACA5150.035.9224661
TTTACAC7750.034.353432
CTTTACA7800.034.1652681
TTTAACG502.3586672E-630.79810534
TTATACA42250.030.7784842
TTACACA8900.030.408963
CTTATAC43400.030.3462851
ACAGTTC300.00574040829.33458543
CGACGTT300.005743332729.33152814
TTTGTCT300.005743332729.33152828
TCGGATA300.005743332729.33152837
TTAACGG454.0661973E-529.33152835
CGGCTAC454.0661973E-529.33152812
TGTCGGA300.005743332729.33152835
CCCGACG300.005743332729.33152812
Produced by FastQC (version 0.11.5)