FastQCFastQC Report
Wed 25 May 2016
SRR1780460_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780460_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82237
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGATC200.006932308452.49406435
TTTGGTC200.006932308452.49406461
ATAACTT350.001249674139.99548317
CAGCAAT400.0023156935.2749821
TACCAAT400.002407767634.99604412
TTTATTG450.004287821731.10759751
GAGGGCT450.004287821731.10759738
TTTACAC500.007176053727.9968362
GAACCAA500.007176053727.99683646
GGGCTAG1000.0091919717.6374911
GACGAAT3800.017.49802270
GAATTGA2108.3568193E-716.66478370
CGAATTG2351.4942088E-716.38112869
AACACAC1554.001754E-415.80466652
AATTGAA1605.094424E-415.31076970
CGACGAA4400.015.11192969
GGCTGTC1450.00469396614.48112254
ACACCAC1500.00587797513.9984185
CCACACC2758.8771594E-713.9984183
GGCTAGG1550.007303599313.5468572
Produced by FastQC (version 0.11.5)