##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780460_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 82237 Sequences flagged as poor quality 0 Sequence length 76 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.099067329790728 33.0 23.0 34.0 12.0 34.0 2 29.421744470250616 34.0 27.0 34.0 12.0 34.0 3 29.610674027505866 34.0 31.0 34.0 11.0 34.0 4 29.898366915135522 34.0 31.0 34.0 11.0 34.0 5 30.013096294855114 34.0 31.0 34.0 11.0 34.0 6 31.947614820579545 37.0 31.0 38.0 11.0 38.0 7 31.568004669431033 37.0 27.0 38.0 11.0 38.0 8 30.1263056774931 35.0 25.0 38.0 11.0 38.0 9 31.18867419774554 36.0 27.0 38.0 12.0 38.0 10-11 31.616182496929607 37.0 29.0 38.0 11.0 38.0 12-13 32.008092464462464 37.0 29.5 38.0 11.0 38.0 14-15 31.65725281807459 37.0 27.5 38.0 11.0 38.0 16-17 31.50047423908946 37.0 27.0 38.0 11.0 38.0 18-19 31.502778554665177 37.0 27.5 38.0 11.0 38.0 20-21 31.6221408854895 37.0 27.5 38.0 11.0 38.0 22-23 31.567348030691782 37.0 27.5 38.0 11.0 38.0 24-25 32.00057151890269 37.0 31.0 38.0 11.0 38.0 26-27 32.05394773642035 37.0 31.0 38.0 11.0 38.0 28-29 32.114133540863605 37.0 31.0 38.0 11.0 38.0 30-31 31.930505733428994 37.0 31.0 38.0 11.0 38.0 32-33 31.80550725342607 37.0 29.0 38.0 11.0 38.0 34-35 31.704105208118 37.0 27.0 38.0 11.0 38.0 36-37 31.60945802984058 37.0 27.0 38.0 11.0 38.0 38-39 32.05576565292995 37.0 31.0 38.0 11.0 38.0 40-41 31.593072461300874 37.0 27.0 38.0 11.0 38.0 42-43 31.964237508663984 37.0 31.0 38.0 11.0 38.0 44-45 30.976987244184492 36.0 26.5 38.0 11.0 38.0 46-47 31.69198779138344 37.0 27.0 38.0 11.0 38.0 48-49 31.516014689251797 37.0 27.0 38.0 11.0 38.0 50-51 31.464334788477206 37.0 27.0 38.0 11.0 38.0 52-53 31.455938324598414 37.0 27.0 38.0 11.0 38.0 54-55 31.179007016306528 36.5 27.0 38.0 11.0 38.0 56-57 31.20704792246799 36.5 27.0 38.0 11.0 38.0 58-59 31.052859418509918 36.5 26.5 38.0 11.0 38.0 60-61 31.0992679694055 36.5 26.5 38.0 11.0 38.0 62-63 31.130902148667875 36.5 27.0 38.0 11.0 38.0 64-65 31.15696097863492 37.0 27.0 38.0 11.0 38.0 66-67 31.19883385823899 36.5 27.0 38.0 11.0 38.0 68-69 30.710525675790702 36.0 26.0 38.0 11.0 38.0 70-71 30.519772122037526 36.0 25.0 38.0 11.0 38.0 72-73 30.622560404684023 36.0 25.0 38.0 11.0 38.0 74-75 30.278578985128348 36.0 25.0 38.0 11.0 38.0 76 29.49345185257244 34.0 24.0 37.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -2.2685988669133756 1101 2 -1.2390408539351796 1101 3 -0.7742361097951154 1101 4 -3.706703880562074 1101 5 -1.440035481086273 1101 6 -3.007425051561569 1101 7 -0.2708700558780741 1101 8 0.22843799395484865 1101 9 -0.710949514873505 1101 10-11 -1.0897480884781672 1101 12-13 -1.1271681388504895 1101 14-15 -0.9446580882558351 1101 16-17 -2.819020788793381 1101 18-19 -0.8289549400798712 1101 20-21 -1.5826264734224793 1101 22-23 -0.4375668451632855 1101 24-25 -1.5160325935358898 1101 26-27 -2.1842442212297506 1101 28-29 -1.1219114361203992 1101 30-31 -2.2462678744834648 1101 32-33 -0.8247982988460265 1101 34-35 -1.5079068894706182 1101 36-37 -0.20902644577922302 1101 38-39 -1.2928855484348034 1101 40-41 0.14171619165079719 1101 42-43 -1.574486579967349 1101 44-45 -0.9883207254148516 1101 46-47 0.3039528234414419 1101 48-49 -2.113809283488731 1101 50-51 -1.200099587174467 1101 52-53 -0.42666676353278987 1101 54-55 -2.4078988981876748 1101 56-57 -0.9924381790868573 1101 58-59 -0.7123345081553651 1101 60-61 -0.3241774324281934 1101 62-63 -2.189544822284237 1101 64-65 -0.7566172200092858 1101 66-67 -2.371182134073912 1101 68-69 -0.7085064268271744 1101 70-71 0.08673594874647605 1101 72-73 -0.7680001744023848 1101 74-75 -1.321661301286941 1101 76 -2.7849857200878994 1102 1 0.2856391655855255 1102 2 0.051033805704697954 1102 3 0.5645869029409418 1102 4 1.718636479560896 1102 5 0.9962842378421399 1102 6 1.3542350274898105 1102 7 2.0038335014341797 1102 8 2.0145600844247653 1102 9 1.8755678325134078 1102 10-11 0.48578990010329903 1102 12-13 -2.982899364146931 1102 14-15 -0.980889972313804 1102 16-17 -2.0343260149681726 1102 18-19 -0.4719061917267773 1102 20-21 0.8603906543772624 1102 22-23 -0.1385989048909977 1102 24-25 1.1951004762928363 1102 26-27 -3.5962995571981295 1102 28-29 0.39752202896524125 1102 30-31 0.04347740439225234 1102 32-33 -6.280880863624244 1102 34-35 -0.914252958860164 1102 36-37 0.472681942450901 1102 38-39 0.8081245525752934 1102 40-41 0.7217557173424964 1102 42-43 0.5136776712403837 1102 44-45 -4.5195021044223225 1102 46-47 -0.05891498507854109 1102 48-49 0.12587011923415048 1102 50-51 -6.327789530081802 1102 52-53 -1.815994826773899 1102 54-55 0.7706254759888722 1102 56-57 -0.5092365980591893 1102 58-59 1.0373361110804709 1102 60-61 0.4609565157492348 1102 62-63 -3.974239596109445 1102 64-65 -0.3376448879934735 1102 66-67 0.3807941505110648 1102 68-69 -5.887140594591774 1102 70-71 0.0876142974509122 1102 72-73 0.11891242990152406 1102 74-75 -5.382706536245223 1102 76 -2.079452123249954 1103 1 0.5577741065184192 1103 2 0.018577344859490807 1103 3 -0.08074659277618679 1103 4 0.1704287906882449 1103 5 -0.2753635517084625 1103 6 -1.3232301614843252 1103 7 0.5193005578219498 1103 8 0.9296942540346365 1103 9 0.9488841155483634 1103 10-11 0.5064024929665294 1103 12-13 -1.1857370209487819 1103 14-15 -0.9021744281251145 1103 16-17 -0.18747423077623182 1103 18-19 -0.3010073974264458 1103 20-21 -0.07460508304814795 1103 22-23 -0.0676067399090634 1103 24-25 0.4268627464182728 1103 26-27 -0.6500790400057497 1103 28-29 0.3175886063208182 1103 30-31 0.004708273551507602 1103 32-33 -4.132157606205336 1103 34-35 -0.6073678859904348 1103 36-37 0.2802571464455461 1103 38-39 0.6251180166275816 1103 40-41 0.6600295776112404 1103 42-43 0.005578779509772147 1103 44-45 -4.321272087777984 1103 46-47 0.06653664484879229 1103 48-49 0.3523650650385619 1103 50-51 0.1639187474320991 1103 52-53 0.0758372683830082 1103 54-55 0.7828956014303543 1103 56-57 1.2005632639145887 1103 58-59 0.8580542534098612 1103 60-61 0.6367978667824019 1103 62-63 0.6761074992504383 1103 64-65 0.8010816171013211 1103 66-67 0.6745058380846451 1103 68-69 -2.902526459082498 1103 70-71 0.04297905204840191 1103 72-73 1.067264234391434 1103 74-75 -1.2130966633105373 1103 76 0.09961660569089759 1104 1 -1.4689210543045306 1104 2 0.19895984268620737 1104 3 0.5847851371571657 1104 4 0.4372355133773205 1104 5 0.785452153884119 1104 6 0.3514655788807879 1104 7 1.8203002680508718 1104 8 1.8853955000502864 1104 9 0.19609506889521455 1104 10-11 -0.33273833577876033 1104 12-13 -0.6502219528525792 1104 14-15 0.27924037917711786 1104 16-17 0.48135364520174306 1104 18-19 0.69422949043215 1104 20-21 1.1448421786151428 1104 22-23 0.13912681558209883 1104 24-25 0.5950567481568996 1104 26-27 1.0190386070530764 1104 28-29 -0.0978344594572178 1104 30-31 -0.33140806953745283 1104 32-33 -0.6109137638408022 1104 34-35 0.5067656984778353 1104 36-37 0.05048679002711509 1104 38-39 -0.09585054321014397 1104 40-41 1.2386118725982058 1104 42-43 0.596729895128437 1104 44-45 -2.243088228027183 1104 46-47 0.5908955263324174 1104 48-49 1.4732901592141658 1104 50-51 1.7614991589070357 1104 52-53 0.7550425711937869 1104 54-55 0.050173202125805005 1104 56-57 0.8046273033234534 1104 58-59 1.0827717266060404 1104 60-61 0.42379801166794095 1104 62-63 0.42100028395052647 1104 64-65 0.6819494814884521 1104 66-67 0.7355750933729723 1104 68-69 -2.721546308401539 1104 70-71 0.014744482358455713 1104 72-73 0.8565644372311922 1104 74-75 -0.917512050154933 1104 76 1.0706394976062832 1105 1 -0.7897944290925452 1105 2 -0.6775240465167336 1105 3 -0.6889570900898647 1105 4 0.4946125625576485 1105 5 -0.243024386534195 1105 6 0.4819771673488482 1105 7 0.7126123087518295 1105 8 0.61115864306894 1105 9 -0.35174269255193025 1105 10-11 1.2346019694784403 1105 12-13 -1.379341511925503 1105 14-15 0.07082460755473008 1105 16-17 1.0966730346873277 1105 18-19 0.6092591682162052 1105 20-21 0.6286253894687306 1105 22-23 0.5288597155255701 1105 24-25 0.8980881218143324 1105 26-27 0.2280550004957007 1105 28-29 -1.2464353639230126 1105 30-31 0.4103598784557647 1105 32-33 0.6283810354808459 1105 34-35 0.9685378861544933 1105 36-37 0.1606125673024117 1105 38-39 0.2348094341782101 1105 40-41 0.24309887090232962 1105 42-43 0.6812055876100622 1105 44-45 1.557258012786832 1105 46-47 0.9265724624545228 1105 48-49 0.8316494017240572 1105 50-51 -0.07025607003586742 1105 52-53 -1.1191448228141283 1105 54-55 -0.33804047791941727 1105 56-57 0.019649914968447035 1105 58-59 0.13824091823973106 1105 60-61 0.23276494213713406 1105 62-63 0.16047173674871829 1105 64-65 0.09673184440535465 1105 66-67 0.48939080846623995 1105 68-69 1.1262137255159317 1105 70-71 1.6210545445404811 1105 72-73 -0.09920153224308947 1105 74-75 -0.6293902299175116 1105 76 -0.47529040629422425 1106 1 -1.001207976880977 1106 2 -0.3807408729936874 1106 3 -0.8838225401362614 1106 4 -1.350500335679282 1106 5 -0.7385393880807456 1106 6 -0.4680692291868809 1106 7 0.531588491863495 1106 8 0.26836557161827557 1106 9 0.8806266238724447 1106 10-11 -0.8282653364293786 1106 12-13 0.27434701266466277 1106 14-15 -0.3697104991572999 1106 16-17 -0.159667825195136 1106 18-19 -0.7449783820467104 1106 20-21 -1.0150878799404879 1106 22-23 -0.5589457283346206 1106 24-25 -0.47679588684635377 1106 26-27 -0.9385085627582441 1106 28-29 -1.2774050515196755 1106 30-31 -0.8022522465055673 1106 32-33 0.21150816198873557 1106 34-35 -0.043331894235244306 1106 36-37 -0.2173645437399614 1106 38-39 -0.043028487256989933 1106 40-41 -0.5019135015071967 1106 42-43 0.2978928749592775 1106 44-45 0.5636375364490647 1106 46-47 0.3432133532680126 1106 48-49 -0.2353549286192802 1106 50-51 0.05590384335726384 1106 52-53 -0.5145979623130472 1106 54-55 -0.16583200281889177 1106 56-57 0.30096757658304796 1106 58-59 -0.30036099948374684 1106 60-61 0.5103708808937029 1106 62-63 0.533963849337642 1106 64-65 0.6072097287233262 1106 66-67 0.5136329983827252 1106 68-69 0.8755177774800522 1106 70-71 0.08675977188350359 1106 72-73 0.051587208864244616 1106 74-75 -0.8648526487235699 1106 76 -0.30980942887387286 1107 1 -0.1140240733344342 1107 2 1.1952397110507818 1107 3 0.9868812882517446 1107 4 0.5855757623399747 1107 5 0.45055088045444336 1107 6 1.017995125310886 1107 7 0.13998119522214125 1107 8 0.7186513306412152 1107 9 -0.13068630183020957 1107 10-11 0.5690179955991184 1107 12-13 0.8832681744903823 1107 14-15 0.20656367292443178 1107 16-17 1.2831314388775183 1107 18-19 1.1784636441021945 1107 20-21 1.2969472723338455 1107 22-23 0.15238872715743312 1107 24-25 0.24943326840300273 1107 26-27 1.0911340427377212 1107 28-29 -0.25009157778601576 1107 30-31 0.8853700818432912 1107 32-33 1.9540901709138119 1107 34-35 0.868816778746826 1107 36-37 0.37114246322786215 1107 38-39 1.035418236300515 1107 40-41 1.0734505476590144 1107 42-43 1.3527840516601337 1107 44-45 0.884871780557738 1107 46-47 1.00709419566995 1107 48-49 0.9331646047714699 1107 50-51 1.7154983004151205 1107 52-53 1.590203180723048 1107 54-55 1.418770283253295 1107 56-57 1.467901734572557 1107 58-59 1.601551237265646 1107 60-61 0.686416054306374 1107 62-63 0.3445408476935512 1107 64-65 0.5295850516701641 1107 66-67 1.7550700139624666 1107 68-69 1.795632472539939 1107 70-71 0.5410089694095355 1107 72-73 1.2610035558461554 1107 74-75 0.8967049336768049 1107 76 -0.16859358294887627 1108 1 -0.4056840039985161 1108 2 0.3922722773779519 1108 3 0.15722132166231617 1108 4 -0.3971800710382638 1108 5 -0.10742364847444108 1108 6 0.5603960162594994 1108 7 1.1338918488838345 1108 8 1.2746140401308956 1108 9 -0.11426841819240963 1108 10-11 -0.011104509834588328 1108 12-13 0.11958510790275767 1108 14-15 0.43918029558255256 1108 16-17 -0.9297711495437397 1108 18-19 0.15372904260411246 1108 20-21 -0.2893364301324368 1108 22-23 -0.684680842277281 1108 24-25 -0.41754774505103853 1108 26-27 -0.4912427782283082 1108 28-29 0.029964465755504932 1108 30-31 0.4157090374934498 1108 32-33 0.05182507777734813 1108 34-35 -0.046507178070903876 1108 36-37 -0.3969773837301602 1108 38-39 0.4387161831669246 1108 40-41 -0.2778364779018716 1108 42-43 0.05141529593452887 1108 44-45 -0.5662428033369338 1108 46-47 0.07487634436496649 1108 48-49 -0.1372580569375046 1108 50-51 0.06072292364804355 1108 52-53 -0.20949504636107363 1108 54-55 0.4990275087387346 1108 56-57 0.21138577473709574 1108 58-59 -1.3407616365824992 1108 60-61 0.8543939961432372 1108 62-63 -0.018188400927815707 1108 64-65 0.44540298201091844 1108 66-67 0.5490920362002569 1108 68-69 1.6223016539809088 1108 70-71 1.0102568722674015 1108 72-73 0.21107630467409422 1108 74-75 0.3955104158847753 1108 76 -0.11759755269973127 1109 1 0.23932032500581357 1109 2 -0.2962125711068957 1109 3 0.6920265164675108 1109 4 0.1512355133773191 1109 5 1.099762498711705 1109 6 0.6845345443980229 1109 7 -0.26735490436292153 1109 8 0.9399127414295947 1109 9 1.5180605861365954 1109 10-11 1.3938478711177922 1109 12-13 1.030347012664663 1109 14-15 0.45511968952194337 1109 16-17 -0.22081876859136074 1109 18-19 0.6898329387080082 1109 20-21 0.964100799304795 1109 22-23 1.3106957810993407 1109 24-25 1.4606946791913842 1109 26-27 0.8960386070530717 1109 28-29 0.7066138164048539 1109 30-31 1.0705402063246225 1109 32-33 1.0884138223660962 1109 34-35 0.8728001812364568 1109 36-37 1.4087109279581504 1109 38-39 1.1112356636864078 1109 40-41 0.9421808381154406 1109 42-43 1.0704023089215404 1109 44-45 0.5636186685245406 1109 46-47 0.3413265608151761 1109 48-49 0.4619280902486409 1109 50-51 1.4428095037346225 1109 52-53 1.525232226366203 1109 54-55 1.0248283745395952 1109 56-57 0.9114204067717253 1109 58-59 0.8849786231577639 1109 60-61 1.1741255978748342 1109 62-63 0.17758649084707656 1109 64-65 0.5781908607987951 1109 66-67 0.8051613002695213 1109 68-69 1.6255743812536387 1109 70-71 0.7373824133929396 1109 72-73 -0.021676942079153605 1109 74-75 1.7562982946726535 1109 76 0.14939811829593808 1110 1 -0.6006971422430922 1110 2 -0.15053571521169928 1110 3 -0.6698075446678615 1110 4 -0.07914003247420709 1110 5 -0.6851283309826179 1110 6 -0.22150912372424614 1110 7 -0.6280885288170701 1110 8 0.940978243613003 1110 9 -0.8741315536013943 1110 10-11 -1.0733835699302432 1110 12-13 -0.05643901353621317 1110 14-15 -0.3131685200850427 1110 16-17 0.02541703926890193 1110 18-19 0.5913176548652146 1110 20-21 -0.16033588191791637 1110 22-23 -0.6202561839661627 1110 24-25 -0.17671143434573366 1110 26-27 -1.6365290785364444 1110 28-29 0.06620333605550854 1110 30-31 0.01467121069143218 1110 32-33 1.1020906782612947 1110 34-35 0.1948962511054475 1110 36-37 -0.1202235698584424 1110 38-39 -0.00434512233979234 1110 40-41 0.13425070711107878 1110 42-43 0.33951147922721603 1110 44-45 0.8706841707079462 1110 46-47 0.34372830754006856 1110 48-49 0.7697534177595529 1110 50-51 0.614608630372178 1110 52-53 -0.16384200944166238 1110 54-55 -0.46268254249096685 1110 56-57 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2116 46-47 -0.4435219240333055 2116 48-49 -0.3006779703574196 2116 50-51 0.09282970575482352 2116 52-53 0.19707061020458028 2116 54-55 -0.6099191002078754 2116 56-57 -0.38132706797574656 2116 58-59 -0.8360718818927424 2116 60-61 -2.094379452630214 2116 62-63 -0.058231690971105365 2116 64-65 0.29136257797051357 2116 66-67 -0.659060921952701 2116 68-69 -0.029213497534243515 2116 70-71 -3.5294256674151434 2116 72-73 -0.15183855824076886 2116 74-75 -2.4226714022970413 2116 76 -1.5021574372596191 2117 1 0.1942562855969463 2117 2 0.10536378357290843 2117 3 0.8938984376497778 2117 4 -0.5566068511547009 2117 5 -0.07846410227351441 2117 6 -1.8917610221044363 2117 7 -2.5700938206190784 2117 8 -3.551180854629518 2117 9 0.5994201920479263 2117 10-11 -0.3025659219856607 2117 12-13 0.9159726284282073 2117 14-15 -0.3811660247637718 2117 16-17 0.23443246293573594 2117 18-19 0.022739342648893768 2117 20-21 0.2184259224575058 2117 22-23 0.34509972198603833 2117 24-25 0.12738433436379637 2117 26-27 0.7166987055752401 2117 28-29 0.42741184596150816 2117 30-31 0.6239490733196931 2117 32-33 1.1683350046321017 2117 34-35 0.17486914675369647 2117 36-37 -0.47198857943100947 2117 38-39 0.8946051218144859 2117 40-41 -1.1243807382392355 2117 42-43 0.1727766931579957 2117 44-45 1.0287713778841407 2117 46-47 -0.5042399416478744 2117 48-49 -0.49992412650012596 2117 50-51 1.057065661370089 2117 52-53 1.4499317337553599 2117 54-55 -1.0169450244751808 2117 56-57 -1.0358899380558597 2117 58-59 -0.5169819679752408 2117 60-61 0.5103127899930655 2117 62-63 0.3538820573495407 2117 64-65 -0.7447746564425834 2117 66-67 -1.0021096356910704 2117 68-69 -0.04205123450990911 2117 70-71 1.056308521767324 2117 72-73 1.5185102500390748 2117 74-75 0.119125880879551 2117 76 0.09034393110382055 2118 1 -0.5184272478097185 2118 2 0.10203985607757105 2118 3 0.17726923491411384 2118 4 0.24063357162974697 2118 5 -0.39713070517179005 2118 6 0.054553961873754275 2118 7 -0.012558787858068854 2118 8 -3.5095241517742863 2118 9 -1.3914928119216583 2118 10-11 -1.6083074686880323 2118 12-13 -0.25477920092757245 2118 14-15 -1.9411521551382478 2118 16-17 -1.4201779918923307 2118 18-19 -0.8385165758551025 2118 20-21 -3.6642681327340405 2118 22-23 -1.5952848014249312 2118 24-25 -0.5952470683814326 2118 26-27 0.4134560827812308 2118 28-29 -0.49559977582815407 2118 30-31 -1.3596345509569332 2118 32-33 -1.3467900611290524 2118 34-35 0.8370720258966458 2118 36-37 -0.4979104312651508 2118 38-39 -0.1681429770902909 2118 40-41 -1.563760909457372 2118 42-43 -1.0302578852532172 2118 44-45 -0.10894443827157829 2118 46-47 -1.4513918857867623 2118 48-49 -1.6114311330523279 2118 50-51 -0.917442923449844 2118 52-53 -1.7595833076143812 2118 54-55 0.11240119007357663 2118 56-57 0.7754592417231834 2118 58-59 -0.39412817295874447 2118 60-61 -2.631253042901861 2118 62-63 -0.2307824411917565 2118 64-65 -0.16852758580314386 2118 66-67 -2.1552853016722295 2118 68-69 0.6335549637779074 2118 70-71 -1.744734091461428 2118 72-73 -1.6788808255743 2118 74-75 -0.004827918919581009 2118 76 -1.179611590441926 2119 1 -0.28610375876381866 2119 2 1.2767717220868207 2119 3 0.4200893436926414 2119 4 0.31288996363910115 2119 5 0.673270352114848 2119 6 0.42361307842943674 2119 7 -1.2528627577660636 2119 8 0.489001746665199 2119 9 0.08377786362350648 2119 10-11 -0.2636599822853505 2119 12-13 -0.630103249115443 2119 14-15 -0.16164470838381462 2119 16-17 -0.5200229570730386 2119 18-19 -1.261685385899323 2119 20-21 -2.688171452004106 2119 22-23 -0.3204665225655816 2119 24-25 -0.9147712893950057 2119 26-27 0.2422061463200933 2119 28-29 0.9696190520069479 2119 30-31 0.1133360178429399 2119 32-33 0.05819392707813975 2119 34-35 0.18452792992755462 2119 36-37 -0.3130377631413275 2119 38-39 -0.8739580535910818 2119 40-41 -0.4670547639787941 2119 42-43 -1.2602050209213935 2119 44-45 -0.9111352581770333 2119 46-47 -0.9416577323785376 2119 48-49 -0.6392865694372212 2119 50-51 0.9627990325304339 2119 52-53 -1.6435740563563144 2119 54-55 -1.8367423060886736 2119 56-57 -0.06757435762617803 2119 58-59 0.1310205079745188 2119 60-61 0.37759156646122705 2119 62-63 -0.3672512054879995 2119 64-65 -0.15486673082423863 2119 66-67 -0.22087011334304663 2119 68-69 -1.3834099119400811 2119 70-71 -0.8988793667117712 2119 72-73 -2.7451010258487862 2119 74-75 -0.4691572027095461 2119 76 -0.03298931625903734 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 2.0 12 12.0 13 77.0 14 339.0 15 877.0 16 1580.0 17 2135.0 18 2394.0 19 2246.0 20 1952.0 21 1585.0 22 1303.0 23 1201.0 24 1188.0 25 1301.0 26 1340.0 27 1580.0 28 1730.0 29 2211.0 30 2675.0 31 3224.0 32 3866.0 33 5027.0 34 6757.0 35 9311.0 36 14185.0 37 12139.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.56508103737642 19.66335493513335 9.40842102684095 33.36314300064928 2 19.869401850748446 26.974476209005676 28.538249206561524 24.61787273368435 3 17.279326823692497 23.023699794496395 27.24929168135997 32.447681700451135 4 12.29373639602612 18.659484173790386 32.87814487396184 36.168634556221654 5 11.826793292556879 37.32991232656833 31.52352347483493 19.31977090603986 6 23.73870642168367 34.0880625509199 17.55900628670793 24.6142247406885 7 23.77883434463806 34.12575847854372 19.470554616535136 22.624852560283085 8 21.242263214854628 33.05446453542809 17.87151768668604 27.83175456303124 9 21.862422024149712 20.639128372873525 19.00847550372703 38.48997409924973 10-11 21.252599195009545 25.98526210829675 26.258253584153117 26.50388511254058 12-13 21.566934591485584 24.288945365224897 25.595534856573078 28.54858518671644 14-15 21.031895618760412 25.73780658341136 22.927027980106278 30.30326981772195 16-17 21.50917470238457 28.206889842771503 24.330897284677214 25.953038170166714 18-19 20.472536692729552 27.138635893819085 25.09515181730851 27.293675596142858 20-21 19.543514476452206 27.518635164220484 25.507983024672594 27.429867334654716 22-23 22.02718970779576 26.946508262704132 24.92856013716454 26.09774189233557 24-25 20.826392013327336 28.545545192553227 25.093327820810586 25.534734973308854 26-27 19.53317849629729 29.378503593273102 26.037550007903988 25.050767902525628 28-29 20.732152194267787 27.602539003125116 27.892554446295463 23.772754356311637 30-31 21.10546347751012 27.162955847124774 27.266315648673956 24.46526502669115 32-33 19.118523292435277 29.32439169716794 26.517261086858714 25.03982392353807 34-35 20.371608886510938 28.265865729537797 26.243053613337064 25.119471770614197 36-37 20.916375840558384 27.425611342826222 26.52698906818099 25.131023748434405 38-39 20.397752836314552 26.912460328076172 27.681578851368606 25.00820798424067 40-41 19.852377883434464 26.390797329669123 27.70285881051108 26.053965976385324 42-43 19.06197940099955 25.926286221530454 28.27680970852536 26.734924668944636 44-45 18.78473193331469 26.03694200907134 28.980264357892434 26.198061699721535 46-47 18.795067913469605 26.531245060009486 28.87629655751061 25.7973904690103 48-49 18.066077313131558 27.123435923003026 28.248841762223815 26.561645001641597 50-51 17.937789559444045 27.30522757396306 27.577003052154144 27.179979814438752 52-53 17.500030399941632 26.869292410958572 28.543113197222663 27.087563991877133 54-55 17.033695295305034 27.38913141286769 27.60679499495361 27.970378296873672 56-57 17.6708780719141 27.051692060751243 27.649962912071206 27.627466955263447 58-59 18.008317424030544 26.3616133857023 27.33015552610139 28.299913664165764 60-61 17.599134209662317 25.822318421148633 27.70285881051108 28.87568855867797 62-63 17.697630020550363 26.25338959349198 27.237131704707128 28.81184868125053 64-65 18.327516811167722 27.015212130792708 25.675182703649206 28.982088354390363 66-67 18.18828507849265 27.799530624901198 24.76379245351849 29.248391843087663 68-69 17.86057370769848 27.410411372010167 25.584590877585516 29.144424042705836 70-71 18.484988508822063 27.97889028053066 25.416783199776255 28.11933801087102 72-73 18.66556416211681 27.833578559529165 25.28423945426025 28.216617824093778 74-75 18.552476379245352 28.615465058307088 24.58443279788903 28.24762576455853 76 18.31657283218016 27.582475041647918 24.85377628074954 29.247175845422372 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 0.5 8 0.0 9 0.0 10 1.5 11 1.5 12 0.0 13 1.0 14 4.0 15 5.5 16 6.0 17 7.0 18 7.0 19 14.0 20 23.5 21 26.0 22 33.0 23 62.5 24 96.5 25 108.0 26 163.0 27 253.5 28 338.0 29 387.0 30 461.0 31 609.5 32 866.5 33 1049.0 34 1215.5 35 1585.5 36 2026.5 37 2264.0 38 2548.5 39 3190.0 40 3924.0 41 4648.5 42 4996.0 43 5089.5 44 5459.5 45 5871.5 46 6007.0 47 6052.0 48 5862.5 49 5432.5 50 5237.0 51 4925.0 52 4288.0 53 3610.0 54 3257.0 55 3011.5 56 2441.5 57 1913.5 58 1710.0 59 1512.5 60 1144.5 61 846.0 62 718.0 63 663.0 64 538.0 65 410.0 66 313.0 67 274.0 68 222.0 69 142.0 70 102.0 71 90.0 72 72.0 73 43.5 74 37.0 75 41.0 76 33.5 77 19.5 78 11.5 79 10.0 80 6.0 81 4.5 82 9.5 83 12.0 84 9.5 85 7.5 86 7.5 87 7.0 88 4.0 89 5.5 90 6.5 91 2.5 92 2.0 93 3.5 94 3.0 95 1.0 96 1.0 97 1.5 98 2.5 99 5.0 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7393265804929655 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 82237.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.60114060581003 #Duplication Level Percentage of deduplicated Percentage of total 1 83.12369148312536 60.348748130403585 2 9.175110962230969 13.322470420856792 3 3.4754208190268825 7.569585466395905 4 1.5509588811657316 4.504055352213724 5 0.9195209781425341 3.337913591205905 6 0.5543924294447701 2.4149713632549825 7 0.3584289422996399 1.821564502596155 8 0.2177372079390336 1.264637571895862 9 0.15074114395779248 0.9849581088804309 >10 0.47399715266728076 4.431095492296655 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0012159976652844826 0.0 2 0.0 0.0 0.0 0.0012159976652844826 0.0 3 0.0 0.0 0.0 0.0012159976652844826 0.0 4 0.0 0.0 0.0 0.006079988326422413 0.0 5 0.0 0.0 0.0 0.006079988326422413 0.0 6 0.0 0.0 0.0 0.006079988326422413 0.0 7 0.0 0.0 0.0 0.006079988326422413 0.0 8 0.0 0.0 0.0 0.007295985991706896 0.0 9 0.0 0.0 0.0 0.00972798132227586 0.0 10 0.0 0.0 0.0 0.010943978987560344 0.0 11 0.0 0.0 0.0 0.010943978987560344 0.0 12 0.0 0.0 0.0 0.010943978987560344 0.0 13 0.0 0.0 0.0 0.012159976652844826 0.0 14 0.0 0.0 0.0 0.01337597431812931 0.0 15 0.0 0.0 0.0 0.01337597431812931 0.0 16 0.0 0.0 0.0 0.017023967313982757 0.0 17 0.0 0.0 0.0 0.020671960309836205 0.0 18 0.0 0.0 0.0 0.024319953305689652 0.0 19 0.0 0.0 0.0 0.03283193696268103 0.0 20 0.0 0.0 0.0 0.034047934627965515 0.0 21 0.0 0.0 0.0 0.03526393229325 0.0 22 0.0 0.0 0.0 0.03647992995853448 0.0 23 0.0 0.0 0.0 0.03769592762381896 0.0 24 0.0 0.0 0.0 0.042559918284956894 0.0 25 0.0 0.0 0.0 0.04620791128081034 0.0 26 0.0 0.0 0.0 0.0559358926030862 0.0 27 0.0 0.0 0.0 0.08998382723105172 0.0 28 0.0 0.0 0.0 0.12889575252015517 0.0 29 0.0 0.0 0.0 0.15078371049527584 0.0 30 0.0 0.0 0.0 0.1751036638009655 0.0 31 0.0 0.0 0.0 0.23468754939990516 0.0 32 0.0 0.0 0.0 0.3100794046475431 0.0 33 0.0 0.0 0.0 0.35628731592835344 0.0 34 0.0 0.0 0.0 0.4146552038620086 0.0 35 0.0 0.0 0.0 0.5155830100806207 0.0 36 0.0 0.0 0.0 0.6773106995634568 0.0 37 0.0 0.0 0.0 0.9545581672483189 0.0 38 0.0 0.0 0.0 1.3230054598295171 0.0 39 0.0 0.0 0.0 1.7644126123277843 0.0 40 0.0 0.0 0.0 2.2921555990612497 0.0 41 0.0 0.0 0.0 2.9694662986247065 0.0 42 0.0 0.0 0.0 3.827960650315551 0.0 43 0.0 0.0 0.0 4.6268711164074565 0.0 44 0.0 0.0 0.0 5.497525444751146 0.0 45 0.0 0.0 0.0 6.29886790617362 0.0 46 0.0 0.0 0.0 7.089266388608534 0.0 47 0.0 0.0 0.0 7.786033050816543 0.0 48 0.0 0.0 0.0 8.486447706020405 0.0 49 0.0 0.0 0.0 9.138222454612887 0.0 50 0.0 0.0 0.0 9.808237168184638 0.0 51 0.0 0.0 0.0 10.547563748677602 0.0 52 0.0 0.0 0.0 11.31729027080268 0.0 53 0.0 0.0 0.0 12.274280433381568 0.0 54 0.0 0.0 0.0 13.146150759390542 0.0 55 0.0 0.0 0.0 13.974245169449274 0.0 56 0.0 0.0 0.0 14.719651738268663 0.0 57 0.0 0.0 0.0 15.388450454175128 0.0 58 0.0 0.0 0.0 16.026849228449482 0.0 59 0.0 0.0 0.0 16.597152133467905 0.0 60 0.0 0.0 0.0 17.172319029147463 0.0 61 0.0 0.0 0.0 17.708573999537922 0.0 62 0.0 0.0 0.0 18.289820883543904 0.0 63 0.0 0.0 0.0 18.950107615793378 0.0 64 0.0 0.0 0.0 19.635930299013825 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTAGATC 20 0.0069323084 52.494064 35 TTTGGTC 20 0.0069323084 52.494064 61 ATAACTT 35 0.0012496741 39.995483 17 CAGCAAT 40 0.00231569 35.274982 1 TACCAAT 40 0.0024077676 34.996044 12 TTTATTG 45 0.0042878217 31.107597 51 GAGGGCT 45 0.0042878217 31.107597 38 TTTACAC 50 0.0071760537 27.996836 2 GAACCAA 50 0.0071760537 27.996836 46 GGGCTAG 100 0.00919197 17.637491 1 GACGAAT 380 0.0 17.498022 70 GAATTGA 210 8.3568193E-7 16.664783 70 CGAATTG 235 1.4942088E-7 16.381128 69 AACACAC 155 4.001754E-4 15.8046665 2 AATTGAA 160 5.094424E-4 15.310769 70 CGACGAA 440 0.0 15.111929 69 GGCTGTC 145 0.004693966 14.481122 54 ACACCAC 150 0.005877975 13.998418 5 CCACACC 275 8.8771594E-7 13.998418 3 GGCTAGG 155 0.0073035993 13.546857 2 >>END_MODULE