FastQCFastQC Report
Wed 25 May 2016
SRR1780460_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780460_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82237
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGT29953.641913007527026No Hit
ATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTCTT28753.4959932876928876TruSeq Adapter, Index 5 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTC9891.2026216909663534No Hit
CTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTC4540.5520629400391551No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCG3140.38182326689932755No Hit
TATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTCT2380.2894074443377069No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATATCGTATGCCGT1920.2334715517346207No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCC1740.21158359375949998No Hit
CTTAATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCG1480.17996765446210344No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCC1410.17145567080511204No Hit
CTTATAACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCG990.12038376886316378No Hit
ATACACATCTCCGAGCCCACGAGACGTGTGGTGATATCGTATGCCGTCTT980.11916777119787929No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCC960.11673577586731035No Hit
ATACACATCTCCGAGCCCACGAGACGTGTGGTGAACTCGTATGCCGTCTT850.10335980154918102No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGTGGTGAACTCGTATGCCGT850.10335980154918102No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATGC150.002217542670.0000151
CTTTATA453.783498E-1062.2222182
TTTATAC553.45608E-1157.2727243
CTTTACA1850.051.081081
CTATACA1100.050.909091
TCTTTAT552.242814E-950.909091
TTTACAC1900.049.736842
TTACACA2000.047.2500043
TTAATAC350.001248980240.02
TAAGGCG459.127552E-538.8888855
AAGGCGA459.127552E-538.8888856
AACTCGT654.7971116E-737.69230737
TGAGAGG501.6967318E-435.0000044
CCTGTTA400.00240643335.014
GAACTCG751.4696398E-632.66666836
AGAGGGC552.96951E-431.818186
TGTGGTC450.00428545331.1111098
CACACAG450.00428545331.11110932
CTAGCAT450.00428545331.1111094
GGTGAAC853.900521E-628.8235333
Produced by FastQC (version 0.11.5)