##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780460_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 82237 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.007940464754306 34.0 34.0 34.0 32.0 34.0 2 33.36040954801367 34.0 34.0 34.0 33.0 34.0 3 33.518477084524 34.0 34.0 34.0 34.0 34.0 4 33.528898184515484 34.0 34.0 34.0 34.0 34.0 5 33.538091126865034 34.0 34.0 34.0 34.0 34.0 6 37.29891654608023 38.0 38.0 38.0 36.0 38.0 7 36.913712805671416 38.0 37.0 38.0 35.0 38.0 8 36.95218697180101 38.0 37.0 38.0 35.0 38.0 9 36.72191349392609 38.0 37.0 38.0 34.0 38.0 10-11 36.46544134635261 38.0 37.0 38.0 34.0 38.0 12-13 36.31792866957696 38.0 37.0 38.0 34.0 38.0 14-15 36.512239016501084 38.0 37.0 38.0 34.0 38.0 16-17 36.629473351411164 38.0 37.0 38.0 34.0 38.0 18-19 36.66560064204677 38.0 37.0 38.0 34.0 38.0 20-21 36.41126865036419 38.0 37.0 38.0 34.0 38.0 22-23 36.46973989809939 38.0 37.0 38.0 34.0 38.0 24-25 35.724740688497874 38.0 36.5 38.0 31.5 38.0 26-27 35.86729817478751 38.0 36.5 38.0 32.0 38.0 28-29 35.7416126561037 38.0 36.5 38.0 31.0 38.0 30-31 35.39937619319771 38.0 36.0 38.0 29.0 38.0 32-33 35.96128871432567 38.0 37.0 38.0 33.0 38.0 34-35 35.854280919780635 38.0 36.5 38.0 32.0 38.0 36-37 35.88802485499228 38.0 37.0 38.0 32.0 38.0 38-39 35.35678587497112 38.0 36.5 38.0 29.0 38.0 40-41 35.23939346036455 37.5 35.5 38.0 29.0 38.0 42-43 35.82949280737381 38.0 36.5 38.0 32.5 38.0 44-45 36.03984824349138 38.0 37.0 38.0 33.0 38.0 46-47 36.282293857995796 38.0 37.0 38.0 34.0 38.0 48-49 36.05063414278244 38.0 37.0 38.0 33.0 38.0 50-51 36.17807677809259 38.0 37.0 38.0 34.0 38.0 52-53 36.070965623746005 38.0 37.0 38.0 34.0 38.0 54-55 36.26391405328502 38.0 37.0 38.0 34.0 38.0 56-57 36.27766090689106 38.0 37.0 38.0 34.0 38.0 58-59 36.3192054671255 38.0 37.0 38.0 34.0 38.0 60-61 36.04014008293104 38.0 37.0 38.0 33.0 38.0 62-63 36.35647579556647 38.0 37.0 38.0 34.0 38.0 64-65 36.34947772900276 38.0 37.0 38.0 34.0 38.0 66-67 35.555406933618684 38.0 36.5 38.0 31.0 38.0 68-69 34.42051023262035 37.5 34.5 38.0 24.0 38.0 70-71 33.79816262752776 37.0 34.0 38.0 22.0 38.0 72-73 33.05353429721415 37.0 33.5 38.0 16.0 38.0 74-75 33.44793705996085 37.5 34.0 38.0 19.5 38.0 76 32.92762381896227 37.0 33.0 38.0 19.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6809619379217295 1101 2 0.6009128479517685 1101 3 0.15124598904392883 1101 4 0.3621795737329947 1101 5 0.25282127439641044 1101 6 -0.9853645114178349 1101 7 0.2513290583932033 1101 8 0.5680710899361969 1101 9 0.5059893756070295 1101 10-11 0.3191741454443786 1101 12-13 0.7887066585507583 1101 14-15 0.7278335192142009 1101 16-17 -0.02987910024452134 1101 18-19 -0.28015765258163583 1101 20-21 -0.24823704207914687 1101 22-23 0.4494113191115048 1101 24-25 -1.9257484377489078 1101 26-27 -0.12192893038571384 1101 28-29 0.21147972530921066 1101 30-31 0.7477951179699502 1101 32-33 0.20643982039684516 1101 34-35 0.10011906148109517 1101 36-37 -0.11342311822859585 1101 38-39 -0.23878684675623418 1101 40-41 0.9491108229741982 1101 42-43 0.4953924900353428 1101 44-45 0.13686083045500652 1101 46-47 0.3495648903819699 1101 48-49 -1.523911235975568 1101 50-51 -2.031216787298945 1101 52-53 -1.9157915114326087 1101 54-55 -1.0710843160157495 1101 56-57 -1.547021427389545 1101 58-59 -1.249906877283962 1101 60-61 -1.6012108630145434 1101 62-63 -0.9704130441709253 1101 64-65 -1.729584484607031 1101 66-67 -2.5101781965841923 1101 68-69 -2.2288736835840908 1101 70-71 -2.0897232921985136 1101 72-73 -2.3608716322540317 1101 74-75 -1.6871612064561106 1101 76 -1.294267498390898 1102 1 -0.16510745162592144 1102 2 0.29414956292761474 1102 3 0.28760962540756907 1102 4 0.270831308471692 1102 5 0.11733598673720991 1102 6 0.2774813383845327 1102 7 -0.022276563038502672 1102 8 0.2551593639809937 1102 9 0.7031786597528367 1102 10-11 0.5189984757034907 1102 12-13 0.6651888719894927 1102 14-15 0.1514171819283021 1102 16-17 -0.04030949110968862 1102 18-19 -0.7239433354977507 1102 20-21 0.4992377053955934 1102 22-23 0.3332497029498853 1102 24-25 -5.093073865943904 1102 26-27 -1.4989601117647098 1102 28-29 0.39911696729427604 1102 30-31 1.4941719295641533 1102 32-33 0.036149965324376865 1102 34-35 0.8760303482619491 1102 36-37 -2.6843497761117803 1102 38-39 -0.9550802152235178 1102 40-41 0.19625618968478165 1102 42-43 0.4233678962716212 1102 44-45 0.7409451519306316 1102 46-47 0.9714357731224155 1102 48-49 -2.87634865363038 1102 50-51 -0.9530437526041737 1102 52-53 -0.5553391618498296 1102 54-55 0.12006632078706758 1102 56-57 0.18409407546509016 1102 58-59 -0.4334817565110143 1102 60-61 -6.766230625860402 1102 62-63 -2.9997279321814716 1102 64-65 -0.2646086391963962 1102 66-67 0.8626808284487453 1102 68-69 -0.85030539197232 1102 70-71 -1.4341457779253517 1102 72-73 -5.750858457023462 1102 74-75 -2.14302418405822 1102 76 0.020181337797069432 1103 1 0.6125891342548115 1103 2 0.345544033759424 1103 3 0.3627730241003775 1103 4 0.39095472015520016 1103 5 0.2094887898874518 1103 6 0.36121048221598073 1103 7 0.7417797841380462 1103 8 0.7310453705575384 1103 9 0.5072626121743866 1103 10-11 0.30775745755709494 1103 12-13 -0.6367028992117341 1103 14-15 -0.48469088448667463 1103 16-17 0.7603339546744152 1103 18-19 0.7746764022524246 1103 20-21 -0.08502933475379137 1103 22-23 0.6427735124736955 1103 24-25 -2.935064285300051 1103 26-27 -0.33542948212155466 1103 28-29 -0.3472801422741867 1103 30-31 1.2713712150754617 1103 32-33 1.2703987372969365 1103 34-35 0.38795540814097507 1103 36-37 -0.9377801337620895 1103 38-39 0.24141432462140955 1103 40-41 0.32533100801791903 1103 42-43 0.18266436848369239 1103 44-45 1.0392035857389317 1103 46-47 1.0326138675485907 1103 48-49 -2.3895423369007887 1103 50-51 -0.27011840855351466 1103 52-53 0.5760597745363185 1103 54-55 0.39587519506141433 1103 56-57 0.5102317769224882 1103 58-59 -0.005042264772285421 1103 60-61 -4.22719762135425 1103 62-63 -1.1736003797112033 1103 64-65 0.07573764424451213 1103 66-67 -0.6076232352057076 1103 68-69 0.14603082367335674 1103 70-71 -0.3153620325431348 1103 72-73 -4.2133883965355 1103 74-75 -2.0254177211832136 1103 76 -3.455034835628826 1104 1 0.21897134231044646 1104 2 0.3116408173004288 1104 3 0.4451766752159969 1104 4 -0.249366925744539 1104 5 -0.1894897670525637 1104 6 0.024170493806821014 1104 7 0.2548557041612298 1104 8 0.12828530101247537 1104 9 0.6945996159414065 1104 10-11 -1.1095310095538835 1104 12-13 0.040709445036306136 1104 14-15 0.5193477999526195 1104 16-17 0.09731961100592201 1104 18-19 0.6413635943706524 1104 20-21 -0.19784083866570512 1104 22-23 0.31026119720276313 1104 24-25 0.34382226932179805 1104 26-27 0.9142083040273903 1104 28-29 1.460565409739722 1104 30-31 0.1829004819546256 1104 32-33 0.46242641043516386 1104 34-35 -0.07259950347884825 1104 36-37 0.8812216048643933 1104 38-39 1.5347894332855603 1104 40-41 1.6018450619153342 1104 42-43 0.2648325805961207 1104 44-45 0.8152829830150807 1104 46-47 0.7550856148682072 1104 48-49 -0.3308818037220007 1104 50-51 0.22102546278813406 1104 52-53 0.67322364008254 1104 54-55 0.54568684385886 1104 56-57 0.48846272447391925 1104 58-59 -0.7494279500809 1104 60-61 0.412961028312516 1104 62-63 0.3895878266058048 1104 64-65 0.6116511475768789 1104 66-67 0.1350238236178285 1104 68-69 1.6043943414942845 1104 70-71 0.8039330993027107 1104 72-73 0.6675156892367369 1104 74-75 -0.003666779427199174 1104 76 -0.5844538446117298 1105 1 0.8188158872510911 1105 2 0.25073069858929387 1105 3 0.35409414271175876 1105 4 0.12683431789594124 1105 5 -0.4275479920384342 1105 6 0.03003538922796878 1105 7 0.6268879257553479 1105 8 -0.3351827600389683 1105 9 0.7184831659696727 1105 10-11 0.5528841967773772 1105 12-13 -0.3935048003000432 1105 14-15 0.4110592188333442 1105 16-17 0.4337738224248042 1105 18-19 0.594055793353121 1105 20-21 0.16567668535916624 1105 22-23 0.1290147302722957 1105 24-25 0.7014042365349127 1105 26-27 0.5583575408843657 1105 28-29 1.1018175295814387 1105 30-31 1.257130272796914 1105 32-33 0.7619953759524023 1105 34-35 -0.6556972988434637 1105 36-37 0.498660836068467 1105 38-39 -0.10105709017401665 1105 40-41 0.179219284640034 1105 42-43 0.4959306586062908 1105 44-45 1.1291608801321047 1105 46-47 0.9378866323922352 1105 48-49 -0.16253104057898327 1105 50-51 0.7280562711544434 1105 52-53 0.3750679023776229 1105 54-55 0.9235132994835098 1105 56-57 0.5227334989227543 1105 58-59 0.2539637966686783 1105 60-61 0.8055203838806335 1105 62-63 -0.04105801850442958 1105 64-65 0.5149510345186599 1105 66-67 0.575597594109631 1105 68-69 0.7347999378764172 1105 70-71 1.044570465046064 1105 72-73 0.9182929645335136 1105 74-75 0.10144203911434602 1105 76 0.4590029106172153 1106 1 -1.0291581306889022 1106 2 0.09986462992242195 1106 3 0.33530354574299537 1106 4 0.07301433645455546 1106 5 -0.20925229145073843 1106 6 0.2409076440996003 1106 7 -0.6427532743683742 1106 8 0.08965485208599944 1106 9 -0.84492543285495 1106 10-11 -0.056302317296236026 1106 12-13 1.012000921939368 1106 14-15 -0.3350357394097685 1106 16-17 -0.00877180083532636 1106 18-19 0.4569540302847628 1106 20-21 -0.012292692927253768 1106 22-23 -0.007159102081558899 1106 24-25 -0.9002815042631127 1106 26-27 0.47967674905016366 1106 28-29 0.23992455092384546 1106 30-31 0.41944082027603 1106 32-33 0.3469010363297613 1106 34-35 0.46416041844698697 1106 36-37 0.8564167902905524 1106 38-39 -0.48607100913474 1106 40-41 0.5925737541729816 1106 42-43 0.6721601668030175 1106 44-45 0.8020266394887372 1106 46-47 0.6245553611271504 1106 48-49 0.8689799399344693 1106 50-51 -0.12815313187680033 1106 52-53 0.23572364008254 1106 54-55 -0.2891374892576053 1106 56-57 -0.22193089946882338 1106 58-59 0.3101036048963266 1106 60-61 0.648932075807636 1106 62-63 0.3029336301191421 1106 64-65 -0.8152763085063981 1106 66-67 0.2600238236178285 1106 68-69 1.1836477572392425 1106 70-71 -0.049962476494300745 1106 72-73 -0.25221430425708036 1106 74-75 -1.107806337006899 1106 76 -0.27630485306976027 1107 1 0.5261147104603978 1107 2 -0.2231996462852024 1107 3 0.15713475219410356 1107 4 0.06430455801565671 1107 5 -0.044529830008634974 1107 6 0.3159683189606355 1107 7 0.6462743620522033 1107 8 -0.16140206484816133 1107 9 -0.2011651572736426 1107 10-11 0.5179460973283625 1107 12-13 0.31317331683484184 1107 14-15 0.43037763254670125 1107 16-17 0.5379674146974338 1107 18-19 0.5390510317722743 1107 20-21 0.9194774472121026 1107 22-23 0.1887130501144867 1107 24-25 0.521986169736735 1107 26-27 0.40022203989804694 1107 28-29 0.9858730360095791 1107 30-31 1.1708011115153596 1107 32-33 1.0887588614295822 1107 34-35 0.5318324610368208 1107 36-37 1.2250655024176922 1107 38-39 0.923856181032022 1107 40-41 0.7731499696274611 1107 42-43 0.9812887975084124 1107 44-45 0.8067338343529045 1107 46-47 1.0977312007889424 1107 48-49 0.873716493602366 1107 50-51 1.2600779023359934 1107 52-53 0.98572364008254 1107 54-55 0.8258413724037226 1107 56-57 1.0238218602587281 1107 58-59 0.7277325879073686 1107 60-61 1.1939293356526264 1107 62-63 0.8571886994059241 1107 64-65 0.9464182816447035 1107 66-67 -0.01072306434897996 1107 68-69 1.5457396698674444 1107 70-71 0.8108278625020162 1107 72-73 1.5555540352089778 1107 74-75 0.4817478006271685 1107 76 1.1777975504376386 1108 1 -0.7021549451951543 1108 2 0.18893593597486102 1108 3 -0.05726771946977749 1108 4 0.33620554775897205 1108 5 0.1453176668928009 1108 6 -0.42908745514078106 1108 7 -0.15992635286220747 1108 8 -0.6115825897174858 1108 9 -0.24292837331071837 1108 10-11 0.16910199537222326 1108 12-13 1.0401496599937659 1108 14-15 0.31585660723728637 1108 16-17 -0.2676135879790067 1108 18-19 0.08203570961173057 1108 20-21 0.9242016303595193 1108 22-23 -0.14943427973409484 1108 24-25 0.7531837411832711 1108 26-27 -0.9675277759845571 1108 28-29 -1.6142778504483601 1108 30-31 0.24851661869145403 1108 32-33 0.6288784928355184 1108 34-35 0.3681565795186188 1108 36-37 0.8519448471393645 1108 38-39 0.6919490839797007 1108 40-41 0.395359019222397 1108 42-43 -0.031303036660183636 1108 44-45 0.5784192720134484 1108 46-47 0.7052647460818235 1108 48-49 0.8909516788873475 1108 50-51 0.27469951861735353 1108 52-53 -0.7999906456317447 1108 54-55 -0.509396004327435 1108 56-57 0.20370007333195872 1108 58-59 0.5127193853422938 1108 60-61 1.2353692235655345 1108 62-63 1.1158062920484042 1108 64-65 0.596173091150547 1108 66-67 0.6052619188559234 1108 68-69 0.9875765328661288 1108 70-71 -1.0309209833962 1108 72-73 -0.9265281979105993 1108 74-75 -1.4844195037144061 1108 76 0.17037896625556925 1109 1 -1.8488562438964493 1109 2 0.18846840350732208 1109 3 0.193831847629788 1109 4 0.21539169494511867 1109 5 0.03399299156812674 1109 6 -0.6092432992966224 1109 7 -0.4544891234249775 1109 8 0.22976805963316593 1109 9 0.5835651331827876 1109 10-11 0.32824485251509117 1109 12-13 0.6060197898638933 1109 14-15 0.7218133171939982 1109 16-17 0.7988885765231615 1109 18-19 0.6501049736809961 1109 20-21 1.0003488165067083 1109 22-23 0.42591636961655865 1109 24-25 0.06132226932179918 1109 26-27 0.07324278678601104 1109 28-29 -0.5546414868120024 1109 30-31 0.21557289574772653 1109 32-33 0.877995375952402 1109 34-35 0.004745324107354065 1109 36-37 1.0381526393471532 1109 38-39 0.6829101229407328 1109 40-41 0.6161209239843046 1109 42-43 1.096160166803017 1109 44-45 0.6848002243943938 1109 46-47 0.5617063045233834 1109 48-49 0.7715837135193837 1109 50-51 0.2823185662363983 1109 52-53 -0.6882763599174595 1109 54-55 -0.7321752251066513 1109 56-57 0.388635138267027 1109 58-59 0.09053756716048156 1109 60-61 0.6866679248642384 1109 62-63 0.349122309364418 1109 64-65 0.9330821820596427 1109 66-67 0.34202382361782924 1109 68-69 -0.08869186540226792 1109 70-71 0.16322620275097677 1109 72-73 -0.4083463797287763 1109 74-75 -0.17978746908237042 1109 76 0.15716684504344158 1110 1 -1.0663584273025641 1110 2 -0.592439893435909 1110 3 0.0076484415162525465 1110 4 0.04234366001062284 1110 5 0.40885762038908524 1110 6 -0.01560137789924454 1110 7 0.33588642242655453 1110 8 0.7408248281921175 1110 9 0.9753555261958908 1110 10-11 -0.3733184662622122 1110 12-13 0.530281798597521 1110 14-15 0.7616211774560142 1110 16-17 0.5868449084008915 1110 18-19 -0.18882952413559906 1110 20-21 0.7435365894324732 1110 22-23 -0.700956992828857 1110 24-25 -1.214310918451126 1110 26-27 0.9838890749956164 1110 28-29 0.38909650445437194 1110 30-31 -0.18840090337890558 1110 32-33 0.832956074642361 1110 34-35 0.9992082062034271 1110 36-37 1.5546329886921342 1110 38-39 1.0584210399713 1110 40-41 -1.276298289989498 1110 42-43 0.20272785239253466 1110 44-45 1.011149569372563 1110 46-47 0.558466129850892 1110 48-49 1.268378473344704 1110 50-51 -0.3261530494841196 1110 52-53 0.35253586715677443 1110 54-55 -0.15375601113285597 1110 56-57 0.519726841323795 1110 58-59 -0.2552440922281676 1110 60-61 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46-47 -0.8600310692139885 2116 48-49 -0.12997184203617707 2116 50-51 -0.7963683024504604 2116 52-53 -0.17336726900836652 2116 54-55 -0.5559328008642339 2116 56-57 -0.2717689021370191 2116 58-59 -2.5352604126374985 2116 60-61 -1.454746216549907 2116 62-63 0.6735263497684656 2116 64-65 -1.7371602421827816 2116 66-67 -1.0834105198165105 2116 68-69 -1.3349342896446927 2116 70-71 1.2890645865893617 2116 72-73 0.22498695360454946 2116 74-75 0.15122263192773033 2116 76 -1.491237195360597 2117 1 0.6641683866454215 2117 2 0.3116408173004288 2117 3 0.2733786456593492 2117 4 0.09422913336876348 2117 5 0.15472205560753594 2117 6 0.3031113805063299 2117 7 0.7542399406144327 2117 8 0.34811288721937217 2117 9 -1.4260900392310063 2117 10-11 -0.48607041842087284 2117 12-13 -1.1132314416631957 2117 14-15 0.08738967187380098 2117 16-17 -0.6572986155950602 2117 18-19 -0.7351758144963441 2117 20-21 0.34187591010276464 2117 22-23 0.34967006419783786 2117 24-25 -1.1521752676240098 2117 26-27 -1.97618578464256 2117 28-29 -1.627488777452399 2117 30-31 -0.04831872986802921 2117 32-33 -1.7712558555746867 2117 34-35 0.8590507428265752 2117 36-37 -0.6120049961208238 2117 38-39 0.2850973150589624 2117 40-41 0.16465294368872918 2117 42-43 -0.08399746866496116 2117 44-45 -0.33619484949723244 2117 46-47 -0.6747173407968177 2117 48-49 -0.2591453505200292 2117 50-51 -0.547139561842414 2117 52-53 0.5275955612648033 2117 54-55 -0.1833771955500012 2117 56-57 0.3449898180699762 2117 58-59 -0.8251668663370566 2117 60-61 -0.03131237070226689 2117 62-63 0.10110260493092937 2117 64-65 -0.5715385075955339 2117 66-67 -0.18948356554473378 2117 68-69 0.43413572080462615 2117 70-71 0.039462655952945624 2117 72-73 1.458156083325413 2117 74-75 1.4837100678634343 2117 76 1.4875904903636439 2118 1 0.2467165716375277 2118 2 0.28268199574033304 2118 3 -0.4222069873216654 2118 4 0.31834315125579593 2118 5 0.2709638653545312 2118 6 0.26559165215968505 2118 7 0.7539768959925013 2118 8 0.5814573800215186 2118 9 -1.4937164202152715 2118 10-11 -0.6363765067082099 2118 12-13 -1.1697860353788272 2118 14-15 0.4869783657376914 2118 16-17 -3.624956083671016 2118 18-19 -0.8699144437947339 2118 20-21 -0.37023370776513076 2118 22-23 0.5169066608786892 2118 24-25 0.7206426576713127 2118 26-27 -2.6668931355440932 2118 28-29 -0.2118065353556915 2118 30-31 -1.413747492601786 2118 32-33 -0.17489782793109754 2118 34-35 0.0642016347869685 2118 36-37 -3.154391049973235 2118 38-39 -0.22540055667091252 2118 40-41 0.10594616670275059 2118 42-43 0.31779123476417936 2118 44-45 -2.415801717353176 2118 46-47 0.1593373724845435 2118 48-49 0.250826431965983 2118 50-51 -0.8184581327927205 2118 52-53 -1.3589365540922174 2118 54-55 -0.7107577493785016 2118 56-57 0.23950892467478724 2118 58-59 -0.5433265105094236 2118 60-61 -0.4613903275629525 2118 62-63 -1.3814407974316936 2118 64-65 -0.1648595655131686 2118 66-67 -0.31036452589673047 2118 68-69 -1.7335073993828587 2118 70-71 -0.014676709870379057 2118 72-73 1.1295176979411252 2118 74-75 0.5939018513059793 2118 76 0.3452833498978123 2119 1 0.826368886993599 2119 2 -0.9613326436130905 2119 3 0.09098368009052393 2119 4 -0.3700114464161359 2119 5 -0.1268656471753289 2119 6 -0.9591071736421739 2119 7 -0.7263006417495816 2119 8 0.17912931617766503 2119 9 -0.5217751809533411 2119 10-11 -0.29574467628072654 2119 12-13 -0.285477592335063 2119 14-15 0.12005415489033311 2119 16-17 -0.10049362242971682 2119 18-19 -0.05441858652842768 2119 20-21 -1.4053579897760144 2119 22-23 -0.35348677174469856 2119 24-25 0.7054060389553101 2119 26-27 0.008855352231030622 2119 28-29 -0.3082331098486506 2119 30-31 0.7559393878943226 2119 32-33 0.44131474768014556 2119 34-35 0.5603893031649463 2119 36-37 -0.41867458578373373 2119 38-39 -1.8345349032372766 2119 40-41 -1.2653241021937092 2119 42-43 -0.11318014733311088 2119 44-45 0.24022430816403073 2119 46-47 0.09616703750767641 2119 48-49 -0.3047827786272208 2119 50-51 0.8144651630950435 2119 52-53 -0.0064229567760989426 2119 54-55 0.18867294243261057 2119 56-57 -0.9748203591151778 2119 58-59 -0.2347818045672625 2119 60-61 0.21677263690612136 2119 62-63 -0.2700190518921204 2119 64-65 -0.46998588076758097 2119 66-67 0.5440552372303955 2119 68-69 0.8537060403568901 2119 70-71 0.31752986767244806 2119 72-73 0.8497688035172999 2119 74-75 1.1941915885092484 2119 76 1.3057113476612443 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 0.0 21 7.0 22 4.0 23 18.0 24 36.0 25 91.0 26 207.0 27 347.0 28 593.0 29 968.0 30 1732.0 31 2396.0 32 3713.0 33 5576.0 34 8323.0 35 11001.0 36 16652.0 37 30572.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.92453518489244 20.87381592227343 10.540267762685895 30.66138113014823 2 19.389082772961075 18.783515935649405 39.10283692255311 22.72456436883641 3 17.1224631248708 24.785680411493612 34.17683038048567 23.91502608314992 4 12.461544073835379 21.02581563043399 34.781181220132055 31.731459075598572 5 11.790313362598344 37.25330447365541 36.37778615465058 14.578596009095662 6 24.27860938506998 37.73848754210392 17.790045843111983 20.19285722971412 7 24.25064143876844 34.2996461446794 19.8341379184552 21.615574498096965 8 21.908629935430525 35.18124445201065 22.115349538528886 20.79477607402994 9 20.98811970281017 19.64201028734025 18.950107615793378 40.4197623940562 10-11 20.946167783357854 25.206415603682043 29.315879713510952 24.53153689944915 12-13 23.306419251675038 20.5625205199606 27.521675158383697 28.609385069980664 14-15 22.964115908897455 23.50827486411226 21.686102362683464 31.84150686430682 16-17 23.944210027116746 27.680362853703322 22.09224558288848 26.28318153629145 18-19 22.228437321400342 26.84010846699174 23.120979607719153 27.810474603888764 20-21 21.263543173997107 25.10852779162664 23.07720369176891 30.550725342607343 22-23 23.03585977114924 28.92068047229349 23.259603341561586 24.783856414995682 24-25 23.71134647421477 30.226661964809026 22.97566788671766 23.086323674258548 26-27 24.453713048870945 28.614249060641804 25.48183907486898 21.450198815618275 28-29 25.61012682855649 24.149105633717184 27.89681043812396 22.343957099602367 30-31 28.800296703430327 22.879604071160184 28.11933801087102 20.200761214538467 32-33 27.345963495750087 23.54901078589929 29.53597529092744 19.56905042742318 34-35 24.89451220253657 25.677006700147135 30.096550214623583 19.331930882692706 36-37 22.714836387514136 27.15383586463514 28.008074224497488 22.123253523353235 38-39 21.363862981383075 24.66043265196931 28.121162007368945 25.85454235927867 40-41 20.083417439838517 25.14622371925046 30.34886973017012 24.421489110740907 42-43 23.736882425185744 23.948466018945243 31.21830806084852 21.096343495020488 44-45 19.87791383440544 25.394287242968495 29.508615343458537 25.219183579167527 46-47 23.736274426353102 22.25458127120396 28.25552974938288 25.753614553060057 48-49 21.51403869304571 21.716502304315576 29.447815460194317 27.321643542444395 50-51 17.814365796417672 22.093461580553768 32.75289711443754 27.339275508591022 52-53 18.982939552756058 22.49595680776293 35.510171820470106 23.010931819010906 54-55 17.581502243515693 22.552500699198657 32.91523280275302 26.950764254532633 56-57 21.279959142478447 22.3299731264516 30.41757359825869 25.972494132811264 58-59 17.70553400537471 27.159307854128922 31.978306601651322 23.156851538845043 60-61 19.191483152352347 32.605761396938114 26.146381798946944 22.05637365176259 62-63 16.175200943614186 36.91525712270632 24.355217237982902 22.554324695696586 64-65 16.58073616498656 37.889879251431836 22.956819922905748 22.57256466067585 66-67 16.180672933107967 37.54453591449104 22.443060909323055 23.831730243077935 68-69 16.306528691464912 35.997178885416545 22.641268528764424 25.05502389435412 70-71 17.185695003465593 33.97254277271788 22.94952393691404 25.89223828690249 72-73 17.839293748556003 31.794690954193367 23.088147670756474 27.277867626494157 74-75 18.540924401425148 31.251139997811205 23.080851684764767 27.127083915998877 76 19.260187020440924 31.418947675620462 23.052883738463223 26.267981565475395 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 0.5 14 1.5 15 4.0 16 4.0 17 3.0 18 8.0 19 11.0 20 20.5 21 32.0 22 29.5 23 44.5 24 71.5 25 81.0 26 121.5 27 201.5 28 287.5 29 334.0 30 428.5 31 615.0 32 829.0 33 951.0 34 1106.5 35 1477.0 36 1870.5 37 2049.0 38 2331.5 39 2989.5 40 3878.0 41 5348.0 42 6305.0 43 7105.0 44 7993.5 45 7562.0 46 7042.0 47 6685.0 48 5760.0 49 4863.0 50 4534.0 51 4235.0 52 3518.5 53 2947.0 54 2793.0 55 2552.5 56 2008.5 57 1530.5 58 1356.0 59 1117.5 60 778.0 61 591.5 62 506.0 63 410.0 64 264.0 65 195.0 66 156.5 67 137.0 68 111.0 69 72.0 70 50.5 71 42.0 72 28.0 73 9.5 74 4.5 75 4.0 76 4.5 77 3.0 78 0.5 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 82237.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.77380011430378 #Duplication Level Percentage of deduplicated Percentage of total 1 64.12081927582818 29.350535646971558 2 17.315304306245515 15.851745564648517 3 7.741147092420901 10.630251589916947 4 3.7005552161092368 6.775538990965137 5 2.173046781606142 4.9734304510135345 6 1.346863958770555 3.699064897795396 7 0.9191615971096884 2.945146345319017 8 0.5791249369072603 2.120699928256138 9 0.4489546529235183 1.849532448897698 >10 1.5992349175145446 10.39799603584761 >50 0.029221900486146164 1.0457579921446551 >100 0.018595754854820283 2.019772122037526 >500 0.0026565364078314693 1.2026216909663534 >1k 0.0053130728156629385 7.1379062952199135 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGT 2995 3.641913007527026 No Hit ATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTCTT 2875 3.4959932876928876 TruSeq Adapter, Index 5 (95% over 21bp) CTTTACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTC 989 1.2026216909663534 No Hit CTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTC 454 0.5520629400391551 No Hit TCTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCG 314 0.38182326689932755 No Hit TATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTCT 238 0.2894074443377069 No Hit CTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATATCGTATGCCGT 192 0.2334715517346207 No Hit CTCTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCC 174 0.21158359375949998 No Hit CTTAATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCG 148 0.17996765446210344 No Hit TCTTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCC 141 0.17145567080511204 No Hit CTTATAACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCG 99 0.12038376886316378 No Hit ATACACATCTCCGAGCCCACGAGACGTGTGGTGATATCGTATGCCGTCTT 98 0.11916777119787929 No Hit TCTTAATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCC 96 0.11673577586731035 No Hit ATACACATCTCCGAGCCCACGAGACGTGTGGTGAACTCGTATGCCGTCTT 85 0.10335980154918102 No Hit CTTATACACATCTCCGAGCCCACGAGACGTGTGGTGAACTCGTATGCCGT 85 0.10335980154918102 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.002431995330568965 0.0 2 0.0 0.0 0.0 0.002431995330568965 0.0 3 0.0 0.0 0.0 0.002431995330568965 0.0 4 0.0 0.0 0.0 0.007295985991706896 0.0 5 0.0 0.0 0.0 0.007295985991706896 0.0 6 0.0 0.0 0.0 0.007295985991706896 0.0 7 0.0 0.0 0.0 0.008511983656991379 0.0 8 0.0 0.0 0.0 0.00972798132227586 0.0 9 0.0 0.0 0.0 0.012159976652844826 0.0 10 0.0 0.0 0.0 0.01337597431812931 0.0 11 0.0 0.0 0.0 0.01337597431812931 0.0 12 0.0 0.0 0.0 0.01337597431812931 0.0 13 0.0 0.0 0.0 0.014591971983413792 0.0 14 0.0 0.0 0.0 0.017023967313982757 0.0 15 0.0 0.0 0.0 0.017023967313982757 0.0 16 0.0 0.0 0.0 0.02310395564040517 0.0 17 0.0 0.0 0.0 0.0279679463015431 0.0 18 0.0 0.0 0.0 0.034047934627965515 0.0 19 0.0 0.0 0.0 0.04377591595024138 0.0 20 0.0 0.0 0.0 0.04620791128081034 0.0 21 0.0 0.0 0.0 0.04742390894609482 0.0 22 0.0 0.0 0.0 0.04985590427666379 0.0 23 0.0 0.0 0.0 0.05107190194194827 0.0 24 0.0 0.0 0.0 0.0559358926030862 0.0 25 0.0 0.0 0.0 0.05958388559893965 0.0 26 0.0 0.0 0.0 0.0705278645865 0.0 27 0.0 0.0 0.0 0.10943978987560343 0.0 28 0.0 0.0 0.0 0.15199970816056033 0.0 29 0.0 0.0 0.0 0.17631966146624997 0.0 30 0.0 0.0 0.0 0.2042876077677931 0.0 31 0.0 0.0 0.0 0.2723834770237241 0.0 32 0.0 0.0 0.0 0.36115130658949135 0.0 33 0.0 0.0 0.0 0.40979121320087064 0.0 34 0.0 0.0 0.0 0.47180709413037925 0.0 35 0.0 0.0 0.0 0.5897588676629741 0.0 36 0.0 0.0 0.0 0.7672945267945086 0.0 37 0.0 0.0 0.0 1.0567019711322154 0.0 38 0.0 0.0 0.0 1.4871651446429224 0.0 39 0.0 0.0 0.0 1.9711322154261464 0.0 40 0.0 0.0 0.0 2.5487311064362754 0.0 41 0.0 0.0 0.0 3.2698177219499738 0.0 42 0.0 0.0 0.0 4.226807884528862 0.0 43 0.0 0.0 0.0 5.119350170847672 0.0 44 0.0 0.0 0.0 6.072692340430707 0.0 45 0.0 0.0 0.0 6.965234626749517 0.0 46 0.0 0.0 0.0 7.834672957427922 0.0 47 0.0 0.0 0.0 8.622639444532266 0.0 48 0.0 0.0 0.0 9.393581964322628 0.0 49 0.0 0.0 0.0 10.11588457750161 0.0 50 0.0 0.0 0.0 10.862507143986283 0.0 51 0.0 0.0 0.0 11.685737563383878 0.0 52 0.0 0.0 0.0 12.547879908070577 0.0 53 0.0 0.0 0.0 13.594853897880515 0.0 54 0.0 0.0 0.0 14.55914004645111 0.0 55 0.0 0.0 0.0 15.463842309422766 0.0 56 0.0 0.0 0.0 16.301664700803773 0.0 57 0.0 0.0 0.0 17.05436725561487 0.0 58 0.0 0.0 0.0 17.754781910818732 0.0 59 0.0 0.0 0.0 18.39804467575422 0.0 60 0.0 0.0 0.0 19.016987487384025 0.0 61 0.0 0.0 0.0 19.605530357381713 0.0 62 0.0 0.0 0.0 20.237849143329644 0.0 63 0.0 0.0 0.0 20.956503763512774 0.0 64 0.0 0.0 0.0 21.692182351009887 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTATGC 15 0.0022175426 70.00001 51 CTTTATA 45 3.783498E-10 62.222218 2 TTTATAC 55 3.45608E-11 57.272724 3 CTTTACA 185 0.0 51.08108 1 CTATACA 110 0.0 50.90909 1 TCTTTAT 55 2.242814E-9 50.90909 1 TTTACAC 190 0.0 49.73684 2 TTACACA 200 0.0 47.250004 3 TTAATAC 35 0.0012489802 40.0 2 TAAGGCG 45 9.127552E-5 38.888885 5 AAGGCGA 45 9.127552E-5 38.888885 6 AACTCGT 65 4.7971116E-7 37.692307 37 TGAGAGG 50 1.6967318E-4 35.000004 4 CCTGTTA 40 0.002406433 35.0 14 GAACTCG 75 1.4696398E-6 32.666668 36 AGAGGGC 55 2.96951E-4 31.81818 6 TGTGGTC 45 0.004285453 31.111109 8 CACACAG 45 0.004285453 31.111109 32 CTAGCAT 45 0.004285453 31.111109 4 GGTGAAC 85 3.900521E-6 28.82353 33 >>END_MODULE