FastQCFastQC Report
Wed 25 May 2016
SRR1780459_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780459_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences574371
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT300.00577837129.2960154
ACGTCTG407.080089E-427.4650155
TACGGAC508.307342E-526.41240138
CGCTACG701.1148368E-625.18321616
ATTCCGC701.119939E-625.17003312
GGGTCTA450.001394208924.4537961
TAAACGG450.001400596224.43461826
TCGTTGT752.0527204E-623.4776934
CATCGTT752.0730495E-623.45110132
ATGACGG602.8611277E-422.01033434
AAACGGC500.00258482821.99115627
CGGTGGT1105.305992E-921.97583843
GATTCCG956.851151E-720.84280811
CCTGCAA755.4136483E-520.55911313
ACGAGAG654.912237E-420.3172324
CCTTACG550.00447088420.0076481
GTAAACG550.004477657420.00241725
AATTAGT550.004491230519.99195926
CGGCTTC1452.910383E-1019.74545712
TCGGTGG1800.019.53748142
Produced by FastQC (version 0.11.5)