FastQCFastQC Report
Wed 25 May 2016
SRR1780459_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780459_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences574371
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT265774.627148654789326No Hit
ATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGTCTT208783.6349328221654646TruSeq Adapter, Index 18 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGTC65011.131846837671122No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG33900.5902108567459011No Hit
CTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGTC33390.5813315783700779No Hit
TATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGTCT20220.3520372720767587No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCC18150.3159978480807701No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCC11970.20840188658549963No Hit
CTTAATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG9690.16870628914064253No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCC7150.12448400075909126No Hit
CTTATAACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG6960.12117603430535316No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT22200.042.01735744
GCCGTCT24600.037.918143
GTTCGCG250.002351950435.19944419
TTTACAC9500.034.967872
CTTTACA10250.032.4318281
CTATACA5200.031.752381
CGGTATG353.216074E-431.42807443
GCTCTAC502.3461089E-630.8209781
TCGGTCA502.3588946E-630.79951529
TTACACA11050.030.4610543
GAAGACG300.0057429829.33286929
TTAGAAC300.0057429829.33286929
CTTATAA1300.028.7888241
TTAATAC1650.027.9995572
TTCGGTC554.955711E-627.99955628
TTGTTCG407.028259E-427.4995659
TTATACA44050.027.4184072
CTTATAC46350.026.265991
TAACGGC609.742549E-625.6662636
CTTTATA2000.025.29962
Produced by FastQC (version 0.11.5)