FastQCFastQC Report
Wed 25 May 2016
SRR1780458_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780458_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142180
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTGC150.0022217769.9929668
TTAAACG150.0022217769.99296652
AGATACG200.006942331352.49472444
GCCGGAA200.006942331352.49472466
AGCTACG200.006942331352.49472453
TCGCCAG352.0794034E-549.99497648
CGCCAGA451.6764698E-646.66197649
GTTTGCG350.001252679539.9959812
GCGGTTT350.001252679539.9959816
TGCGGTT350.001252679539.9959815
TTTGCGG350.001252679539.9959813
TTCGATG350.001252679539.9959821
CTCTAGC459.166692E-538.884982
TTAGCCC501.7039744E-434.99648310
TCGATGA400.002413521334.99648322
GCTCGCC501.7039744E-434.99648346
CTCGCCA552.9821272E-431.81498347
TTTCGAT450.00429800731.10798320
CACGATT450.00429800731.10798325
AACGCCT450.00429800731.10798355
Produced by FastQC (version 0.11.5)