##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780458_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 142180 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.728688985792658 34.0 23.0 34.0 12.0 34.0 2 29.864108876072585 34.0 31.0 34.0 12.0 34.0 3 30.051821634547757 34.0 31.0 34.0 11.0 34.0 4 30.646609931073286 34.0 32.0 34.0 21.0 34.0 5 30.68697425798284 34.0 32.0 34.0 21.0 34.0 6 32.77418061612041 37.0 32.0 38.0 11.0 38.0 7 31.68381628921086 37.0 27.0 38.0 11.0 38.0 8 31.180292586861725 36.0 27.0 38.0 11.0 38.0 9 32.19314249542833 37.0 31.0 38.0 12.0 38.0 10-11 32.50621395414264 37.0 31.5 38.0 11.0 38.0 12-13 32.91374314249543 37.0 33.0 38.0 11.0 38.0 14-15 32.58340483893656 37.0 31.5 38.0 11.0 38.0 16-17 32.46659164439443 37.0 31.0 38.0 11.5 38.0 18-19 32.448406948937965 37.0 31.5 38.0 11.0 38.0 20-21 32.510219440146294 37.0 31.0 38.0 11.0 38.0 22-23 32.446012097341395 37.0 31.5 38.0 11.0 38.0 24-25 32.88935152623435 37.0 33.5 38.0 11.0 38.0 26-27 32.97059713039809 37.0 34.0 38.0 11.5 38.0 28-29 33.032103671402446 37.0 34.0 38.0 11.0 38.0 30-31 32.89506611337741 37.0 34.0 38.0 11.0 38.0 32-33 32.72365311576874 37.0 32.5 38.0 11.0 38.0 34-35 32.58784639189759 37.0 31.5 38.0 11.0 38.0 36-37 32.56617316078211 37.0 32.0 38.0 11.0 38.0 38-39 33.01159797439865 37.0 34.0 38.0 11.0 38.0 40-41 32.56622591081727 37.0 31.5 38.0 11.0 38.0 42-43 32.88261710507807 37.0 34.0 38.0 11.0 38.0 44-45 31.985497256998173 37.0 29.0 38.0 11.0 38.0 46-47 32.64904346602897 37.0 32.0 38.0 11.0 38.0 48-49 32.44019201012801 37.0 31.5 38.0 11.0 38.0 50-51 32.40560908707272 37.0 31.5 38.0 11.0 38.0 52-53 32.365796877197916 37.0 31.0 38.0 11.0 38.0 54-55 32.09629343086229 37.0 31.0 38.0 11.0 38.0 56-57 32.132479954986636 37.0 31.0 38.0 11.0 38.0 58-59 32.01100365733577 37.0 29.0 38.0 11.0 38.0 60-61 32.08379167252778 37.0 31.0 38.0 11.0 38.0 62-63 32.11354972569981 37.0 31.0 38.0 11.0 38.0 64-65 32.13823322548882 37.0 31.0 38.0 11.0 38.0 66-67 32.155275003516664 37.0 31.0 38.0 11.0 38.0 68-69 31.69752778168519 37.0 29.0 38.0 11.0 38.0 70-71 31.56346532564355 37.0 27.5 38.0 11.0 38.0 72-73 31.68429807286538 37.0 28.5 38.0 11.0 38.0 74-75 31.352377268251512 36.5 27.5 38.0 11.0 38.0 76 30.79004782669855 36.0 26.0 37.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -2.397240361854447 1101 2 -0.9043256022940867 1101 3 -0.1722079895953641 1101 4 -2.174119891271996 1101 5 -0.7585742666277646 1101 6 -2.0899893162153873 1101 7 -0.6202382595933287 1101 8 -0.7737248981833069 1101 9 -0.6589267887483032 1101 10-11 -0.6249424075231929 1101 12-13 -0.9077965394018221 1101 14-15 0.00439929809085271 1101 16-17 -1.287811806541388 1101 18-19 -0.08065342831422129 1101 20-21 -0.7332644338364105 1101 22-23 -0.36471463799382065 1101 24-25 -1.6547268911428965 1101 26-27 -0.9047277411766856 1101 28-29 -0.3869033856813502 1101 30-31 -1.026751461675488 1101 32-33 0.13610332824087834 1101 34-35 -0.739159195728142 1101 36-37 0.055060269384334504 1101 38-39 -0.6567612325897514 1101 40-41 0.445011449868737 1101 42-43 -0.13059902045159788 1101 44-45 0.3624277291485498 1101 46-47 0.39166285883222685 1101 48-49 -0.7882791237775173 1101 50-51 -0.952987569937612 1101 52-53 -0.5306388569546847 1101 54-55 -1.0587219235065959 1101 56-57 -0.6807592608715041 1101 58-59 -0.4816847778981028 1101 60-61 -0.2947213212201589 1101 62-63 -1.4819016601901325 1101 64-65 -0.14497686653100317 1101 66-67 -0.6335990832414211 1101 68-69 -0.03812107146318766 1101 70-71 0.18287281216714035 1101 72-73 -0.5154821152638185 1101 74-75 -1.2894658108712207 1101 76 -1.9432169425545318 1102 1 0.944592522242587 1102 2 -0.3725735807307444 1102 3 0.562976197286936 1102 4 0.5664551311898194 1102 5 0.19292618260853445 1102 6 0.8352937026525353 1102 7 1.918288245348272 1102 8 1.6740738439550533 1102 9 1.5453140019075775 1102 10-11 -0.5737654084216643 1102 12-13 -3.9826842303272443 1102 14-15 -0.8419259489891147 1102 16-17 -2.0647749691649295 1102 18-19 -0.7412733744058677 1102 20-21 0.0940131941959379 1102 22-23 -1.092764952459227 1102 24-25 0.15114911963877375 1102 26-27 -5.043029627969133 1102 28-29 -0.15224031290507156 1102 30-31 0.2176151151439143 1102 32-33 -6.277652287212852 1102 34-35 -0.5525015137874405 1102 36-37 0.32754005375091566 1102 38-39 -0.006240118483731294 1102 40-41 0.4273115397159941 1102 42-43 0.3867235312105777 1102 44-45 -4.949198506251271 1102 46-47 -0.7071960809701068 1102 48-49 -0.06281641039027619 1102 50-51 -6.112385593297898 1102 52-53 -1.6806927653104822 1102 54-55 0.8943957764035559 1102 56-57 -1.2241105636567724 1102 58-59 0.6819360666661431 1102 60-61 0.16500913700086528 1102 62-63 -4.242108308887346 1102 64-65 -0.4687953750664917 1102 66-67 -0.2241471515253366 1102 68-69 -7.9670339196213185 1102 70-71 -0.8527156873836219 1102 72-73 0.05258616865352295 1102 74-75 -5.782313969002395 1102 76 -1.6527766909822041 1103 1 -0.7120256982465989 1103 2 -0.4834491567676089 1103 3 -0.07010427063223901 1103 4 -0.2360687964953989 1103 5 -0.09928934380054244 1103 6 -1.0308105157097458 1103 7 0.4276962566917675 1103 8 1.0055874915977334 1103 9 0.7277174049561452 1103 10-11 0.5650789730033665 1103 12-13 -1.5270124827200107 1103 14-15 -0.4394729181489865 1103 16-17 -0.6917086806147665 1103 18-19 -0.23813973385287568 1103 20-21 0.011269486290935049 1103 22-23 -0.0743530417892515 1103 24-25 0.6183664184689732 1103 26-27 -0.6141710671750857 1103 28-29 0.22570722342955918 1103 30-31 0.2760482948674152 1103 32-33 -5.388456966404625 1103 34-35 -0.7743200178640066 1103 36-37 0.5671394865761528 1103 38-39 0.5126326216793373 1103 40-41 0.40937463790812245 1103 42-43 0.36004504840306595 1103 44-45 -4.791176528229293 1103 46-47 -0.2616058647347259 1103 48-49 0.2336281128284341 1103 50-51 -0.18861033629683277 1103 52-53 -0.06480478232572295 1103 54-55 0.5883199167793052 1103 56-57 1.2286544133017543 1103 58-59 0.5626095866944958 1103 60-61 0.8173274638353263 1103 62-63 0.40150034052775396 1103 64-65 0.8854123526896203 1103 66-67 -0.2961925255061928 1103 68-69 -3.2212132886393974 1103 70-71 0.735504801804602 1103 72-73 0.9061239212235357 1103 74-75 -0.7898715726890302 1103 76 0.4636997358168031 1104 1 -1.8719848587097943 1104 2 -0.43985036644502884 1104 3 -0.06081844557020588 1104 4 0.27227358357077236 1104 5 -0.03903810310575295 1104 6 -0.7327271306808001 1104 7 0.8948953882054127 1104 8 1.6110530106217205 1104 9 0.41089435905043814 1104 10-11 0.03767060348310025 1104 12-13 -0.13443274223200063 1104 14-15 0.6018835748204125 1104 16-17 0.050091102263642995 1104 18-19 0.727670077975084 1104 20-21 1.2799283727673654 1104 22-23 0.3662454642074451 1104 24-25 0.5163128101149681 1104 26-27 0.9068141220308661 1104 28-29 -0.1510051938574577 1104 30-31 -0.41909619437989676 1104 32-33 -0.3945570491176156 1104 34-35 0.1565758671649462 1104 36-37 0.5899954108937706 1104 38-39 0.48956345294484294 1104 40-41 1.433710349239803 1104 42-43 0.8609497216867652 1104 44-45 -2.3806196372036545 1104 46-47 0.3585702880775088 1104 48-49 1.047510970562108 1104 50-51 1.237190299559245 1104 52-53 0.77606318707047 1104 54-55 0.08996125259402987 1104 56-57 0.6146171149146582 1104 58-59 0.7219807095232831 1104 60-61 0.36118473223896075 1104 62-63 0.830942286350755 1104 64-65 0.5619561130287494 1104 66-67 0.3682189199032351 1104 68-69 -2.9559178481927475 1104 70-71 0.45247776499733305 1104 72-73 1.2003689067487606 1104 74-75 -0.6995237904309661 1104 76 0.31680664235112843 1105 1 -1.1698912118451084 1105 2 0.08002896523813874 1105 3 -0.9934453020058491 1105 4 -0.42525632567015137 1105 5 -0.2813029545909025 1105 6 -0.2952013468524157 1105 7 0.7145117248390775 1105 8 -0.07889645307464832 1105 9 -0.06922630926639073 1105 10-11 0.32094463401115547 1105 12-13 -1.9736318965224342 1105 14-15 -0.6600801215492282 1105 16-17 0.41155459236265557 1105 18-19 0.01884685190248092 1105 20-21 -0.7758807237672869 1105 22-23 -0.14583425938992178 1105 24-25 -0.11551990440649007 1105 26-27 0.15979215420908588 1105 28-29 -0.6596943440224692 1105 30-31 -0.33202420593104875 1105 32-33 0.34347925451275074 1105 34-35 0.35980400247846944 1105 36-37 0.24864330693337422 1105 38-39 0.10296073017256191 1105 40-41 -0.03478181247636769 1105 42-43 0.47718322003660774 1105 44-45 0.9459712250075683 1105 46-47 0.7263894918728937 1105 48-49 0.6266673236974114 1105 50-51 -0.2906175593516451 1105 52-53 -1.123740855833816 1105 54-55 -1.2933411401452375 1105 56-57 -0.753459927164549 1105 58-59 -0.15263253305097635 1105 60-61 -0.2726075532395882 1105 62-63 -0.048982424870374075 1105 64-65 -0.2506341303705959 1105 66-67 -0.12737204544330183 1105 68-69 0.2183550478468561 1105 70-71 0.7260184003108563 1105 72-73 0.5173810767157541 1105 74-75 -0.9346336295398778 1105 76 -1.751984611774283 1106 1 0.04289733720478495 1106 2 0.47368984943240733 1106 3 -0.8965383271858052 1106 4 0.24303292665340948 1106 5 0.49813111694995627 1106 6 -0.6970739853697481 1106 7 0.7412568088182283 1106 8 0.8100069915873682 1106 9 1.4755487810560126 1106 10-11 0.777424404550878 1106 12-13 0.6515577115748599 1106 14-15 0.16920019506181916 1106 16-17 0.5238680869433026 1106 18-19 -0.14257858729882145 1106 20-21 -0.1408694162298474 1106 22-23 0.040271259239936796 1106 24-25 0.6213281884807955 1106 26-27 0.4151597870726462 1106 28-29 -0.6979744951573608 1106 30-31 0.10361591100544132 1106 32-33 1.054723359424635 1106 34-35 1.4967424177684734 1106 36-37 0.5757414067155011 1106 38-39 0.5051884529448429 1106 40-41 0.6024806602890109 1106 42-43 0.993058588910543 1106 44-45 1.3479921025827863 1106 46-47 1.1837828286996128 1106 48-49 0.6245333707478053 1106 50-51 0.7091066470522804 1106 52-53 0.21415801065450069 1106 54-55 0.13959275212977929 1106 56-57 1.1522324526583887 1106 58-59 -0.15286695760763536 1106 60-61 0.9292630168257787 1106 62-63 1.1864074093776793 1106 64-65 1.0577306893982907 1106 66-67 1.6148023112681429 1106 68-69 1.981386295261295 1106 70-71 1.0172047264643638 1106 72-73 0.5963545729511743 1106 74-75 -0.8921269415451718 1106 76 -0.3162307299794165 1107 1 -0.07225977799954464 1107 2 0.40868549917240315 1107 3 0.3989084006767669 1107 4 0.1820483515288025 1107 5 0.13718768381918522 1107 6 0.9494825646380107 1107 7 0.3519928458325339 1107 8 0.16947335969355493 1107 9 -0.385242898818813 1107 10-11 -0.016767829729175787 1107 12-13 0.17796952269939226 1107 14-15 0.15071327619248365 1107 16-17 0.6707213988738161 1107 18-19 1.2624070130033331 1107 20-21 0.6103328067867366 1107 22-23 -0.1428375916844118 1107 24-25 -0.21432303532490238 1107 26-27 0.3756870790526534 1107 28-29 -0.3530393443820685 1107 30-31 0.16802673741994312 1107 32-33 1.1273356062496163 1107 34-35 0.24592463633362627 1107 36-37 -0.14284735545005134 1107 38-39 0.842960850039276 1107 40-41 1.0379652927426335 1107 42-43 0.9901617878691695 1107 44-45 0.3639734065816569 1107 46-47 0.5204794638240813 1107 48-49 0.5129947276242532 1107 50-51 0.8898177965326113 1107 52-53 0.7933023920745086 1107 54-55 0.6575434761614289 1107 56-57 0.33097418210594043 1107 58-59 0.8989820715087582 1107 60-61 -0.2863952213526417 1107 62-63 -0.3501954759575625 1107 64-65 0.6962409445460978 1107 66-67 1.0537705240194626 1107 68-69 1.0629418673036213 1107 70-71 0.3170421818657694 1107 72-73 0.6106733357237424 1107 74-75 0.34974414237775164 1107 76 -0.6921471510306283 1108 1 -0.6438916653255689 1108 2 -0.3293820140277255 1108 3 -0.3543338717788558 1108 4 0.13878519298553016 1108 5 0.3214103701766895 1108 6 0.3715786899298905 1108 7 1.3723922075438324 1108 8 0.7239466174919507 1108 9 0.04235507787995374 1108 10-11 0.30630729241439525 1108 12-13 0.36189955738631596 1108 14-15 0.7507385366524701 1108 16-17 -0.2048985605862299 1108 18-19 0.4521253896799209 1108 20-21 0.4580553606808522 1108 22-23 -0.2555639346475189 1108 24-25 -0.4505884303430534 1108 26-27 -0.6823934956536135 1108 28-29 0.3033721916387293 1108 30-31 0.8628495753402134 1108 32-33 1.1013080908314947 1108 34-35 0.6675093913379726 1108 36-37 0.6058788396469552 1108 38-39 1.0102775114690203 1108 40-41 0.5426877665426062 1108 42-43 0.9128740219412208 1108 44-45 0.6640984766640301 1108 46-47 1.0386454312072786 1108 48-49 0.5698153598750793 1108 50-51 0.4363494121546694 1108 52-53 0.5817645229483333 1108 54-55 0.7153190770215119 1108 56-57 1.147845088833236 1108 58-59 -0.5122551365326977 1108 60-61 1.133707792035402 1108 62-63 0.02478373037110515 1108 64-65 0.59947798007709 1108 66-67 0.22916437155718228 1108 68-69 1.0501452879395643 1108 70-71 1.7032530512568727 1108 72-73 0.8183913304128829 1108 74-75 1.2122625486275567 1108 76 1.014246209781156 1109 1 -0.33452946408342754 1109 2 -0.979345800953844 1109 3 0.11170595619301693 1109 4 -0.3151857468238539 1109 5 0.284088471201553 1109 6 -0.10415214117617921 1109 7 -1.5250510853462238 1109 8 -0.6095533600751715 1109 9 0.2763107633325532 1109 10-11 0.07838284634623705 1109 12-13 -0.4171750543646695 1109 14-15 -0.617679817287069 1109 16-17 -1.1912305287187266 1109 18-19 -0.8211006504044285 1109 20-21 -0.07927935708150358 1109 22-23 0.163104065173016 1109 24-25 0.23660431935090287 1109 26-27 -0.024969174568632013 1109 28-29 -0.08735726144771405 1109 30-31 -0.006148828720782262 1109 32-33 0.682793916457527 1109 34-35 -0.3174349430785526 1109 36-37 0.0092924826821843 1109 38-39 0.011485682163986155 1109 40-41 -0.1305869849331529 1109 42-43 0.2380225596380683 1109 44-45 -0.4973381248191018 1109 46-47 -0.7827664300165225 1109 48-49 -0.7870949278425954 1109 50-51 0.16596273343833445 1109 52-53 0.3794295808572059 1109 54-55 -0.40123574572670506 1109 56-57 -0.23319656581581683 1109 58-59 0.4423246956693738 1109 60-61 0.21154026330529518 1109 62-63 -0.5884990969406019 1109 64-65 -0.23731642580417045 1109 66-67 -0.06375132568870612 1109 68-69 1.0356792865679552 1109 70-71 -0.128487547765058 1109 72-73 -0.7366653606735731 1109 74-75 0.8496831522642481 1109 76 -0.8239101922416481 1110 1 0.37989443682285184 1110 2 0.44263996860651744 1110 3 0.1862015286565999 1110 4 -0.07504707794125665 1110 5 0.3105108659664104 1110 6 0.10890195007521442 1110 7 0.9274340479992276 1110 8 2.059640854264334 1110 9 -1.0313575739392533 1110 10-11 -0.7481836705718834 1110 12-13 -0.3104824630223817 1110 14-15 -0.3508655660730611 1110 16-17 0.10783916927395154 1110 18-19 0.6604718391434687 1110 20-21 -0.15205455249036248 1110 22-23 0.06656494444798966 1110 24-25 0.4156631108022495 1110 26-27 -1.3093182877629488 1110 28-29 -0.06544355296398408 1110 30-31 -0.34400491431116365 1110 32-33 0.9091371089579852 1110 34-35 0.687557396374558 1110 36-37 -0.11176038807530375 1110 38-39 0.18946680346030575 1110 40-41 0.24465321865561407 1110 42-43 0.7289694811369429 1110 44-45 1.5500209341021858 1110 46-47 0.40398212226995156 1110 48-49 0.739252809050079 1110 50-51 0.3208323743015171 1110 52-53 -0.08245270640031777 1110 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0.3638663883534434 2116 44-45 1.8191824461296804 2116 46-47 0.17385153807751408 2116 48-49 0.36546930389543775 2116 50-51 0.26047154955924157 2116 52-53 1.48902152040381 2116 54-55 0.3825862525940309 2116 56-57 -0.041539135085344014 2116 58-59 -0.4218734571433842 2116 60-61 -1.1724194344277024 2116 62-63 0.8264631196840853 2116 64-65 0.6372998630287441 2116 66-67 -0.6087185800967632 2116 68-69 0.2537279851405856 2116 70-71 -2.3383347350026717 2116 72-73 0.07534807341543015 2116 74-75 -0.9617425404309685 2116 76 -1.1954433576488697 2117 1 -0.13266401432429475 2117 2 0.5595901176398534 2117 3 0.539376348859502 2117 4 -0.4927642700985473 2117 5 -0.9855732489943456 2117 6 -1.104653246949585 2117 7 -2.1524355136513513 2117 8 -2.5476768191749173 2117 9 -0.10756966614849972 2117 10-11 -0.11426559788736768 2117 12-13 0.4047920864594161 2117 14-15 -0.4732711555067368 2117 16-17 0.20171991526098765 2117 18-19 0.4783401155524558 2117 20-21 0.29966685088407274 2117 22-23 0.5379512279121244 2117 24-25 0.44924688394343804 2117 26-27 1.1253258096966476 2117 28-29 0.7595482433662397 2117 30-31 -0.46879640437104797 2117 32-33 0.8058187245340207 2117 34-35 -0.8256163300057047 2117 36-37 -1.4846274339338166 2117 38-39 0.07150145029232391 2117 40-41 -1.108080654296888 2117 42-43 -1.302871012177068 2117 44-45 0.2791152489590374 2117 46-47 -0.6923836520197462 2117 48-49 -0.6111566907995254 2117 50-51 0.9996757935910736 2117 52-53 0.43934512783086177 2117 54-55 -0.9250282478480507 2117 56-57 -1.0070775966238017 2117 58-59 -0.47743490718758963 2117 60-61 0.726286135864072 2117 62-63 -0.4635634055148543 2117 64-65 -0.8174198540358013 2117 66-67 -1.7452384739959683 2117 68-69 -0.3239342606595912 2117 70-71 0.3128129219380895 2117 72-73 0.7996380822571645 2117 74-75 -5.329913593499214E-4 2117 76 -0.5666758952262363 2118 1 0.4051786028286699 2118 2 0.02325059509343319 2118 3 -0.08962453531379566 2118 4 0.180887365622052 2118 5 -0.019967590285240533 2118 6 0.7988834462422787 2118 7 1.1165300035900287 2118 8 -3.2131056432244307 2118 9 -0.8324149358213582 2118 10-11 -0.9344247490810034 2118 12-13 0.2451545975115863 2118 14-15 -0.7378600149231787 2118 16-17 -0.38232075671071897 2118 18-19 -1.037353960486449 2118 20-21 -3.01279999261725 2118 22-23 -1.0117393114335869 2118 24-25 -1.2050413565516997 2118 26-27 -0.2522603971999047 2118 28-29 -0.8598593605241192 2118 30-31 -1.2557548482260472 2118 32-33 -0.9118647414253047 2118 34-35 0.3963995851136559 2118 36-37 -0.4771119609011052 2118 38-39 -0.7178884701320811 2118 40-41 -1.5016261892217315 2118 42-43 -0.8981848936978452 2118 44-45 0.03831065125788058 2118 46-47 -0.6525074362814607 2118 48-49 -1.0195563371302043 2118 50-51 -0.5123489632612674 2118 52-53 -1.5929271975449133 2118 54-55 -0.009157337149552802 2118 56-57 0.5826659931197824 2118 58-59 0.236844491574562 2118 60-61 -1.2894963575046248 2118 62-63 -0.12302405980309672 2118 64-65 -0.8209565472276665 2118 66-67 -2.313231400609588 2118 68-69 0.7067023441149445 2118 70-71 -1.0200783247462617 2118 72-73 -1.0831647470973884 2118 74-75 0.08394976726134118 2118 76 -0.2702125884181008 2119 1 0.46017056817485624 2119 2 0.9301045041271792 2119 3 0.9110182369911008 2119 4 0.6700440763046487 2119 5 1.0238876462130477 2119 6 1.149844111371884 2119 7 -1.2955848570261956 2119 8 0.7814307921839401 2119 9 0.5417484121839564 2119 10-11 0.5560594261443157 2119 12-13 1.0237579650795325 2119 14-15 0.42976731687308956 2119 16-17 0.3645282616641907 2119 18-19 -0.615831568255615 2119 20-21 -0.5083145597939414 2119 22-23 0.6780252382764687 2119 24-25 0.4430058732394002 2119 26-27 0.6487142412406754 2119 28-29 1.0030380055067596 2119 30-31 0.34909039735489245 2119 32-33 0.8750841861230754 2119 34-35 0.5997950996808328 2119 36-37 -0.24973125804356044 2119 38-39 0.02153722678680481 2119 40-41 0.44019593052954065 2119 42-43 -0.45237339366290996 2119 44-45 -0.4344378990111011 2119 46-47 0.32176071155617336 2119 48-49 -0.47308110482391186 2119 50-51 0.9887549283058377 2119 52-53 0.17406784192969127 2119 54-55 -0.05097596357308731 2119 56-57 1.2378396115086687 2119 58-59 1.7914417108856817 2119 60-61 0.8936623094415097 2119 62-63 0.6694058989756329 2119 64-65 1.009826656852546 2119 66-67 0.8051015833909716 2119 68-69 -0.15740371331536096 2119 70-71 0.4098105874314797 2119 72-73 -1.212742753105914 2119 74-75 0.24124383559083284 2119 76 1.1337628185545796 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 3.0 12 20.0 13 143.0 14 603.0 15 1353.0 16 2092.0 17 2771.0 18 2990.0 19 2929.0 20 2707.0 21 2208.0 22 1847.0 23 1709.0 24 1715.0 25 1883.0 26 2079.0 27 2447.0 28 2872.0 29 3339.0 30 4136.0 31 5244.0 32 6391.0 33 8257.0 34 11494.0 35 16255.0 36 26468.0 37 28225.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.24199502589792 20.88768590883647 10.077870914256968 31.79244815100864 2 18.089745393163597 21.947531298354196 32.59389506259671 27.368828245885496 3 16.47348431565621 24.35926290617527 29.50414966943311 29.663103108735406 4 11.962301308200871 22.37023491348994 36.30960753973836 29.35785623857082 5 11.231537487691659 38.70516247010831 33.52440568293712 16.53889435926291 6 23.383738922492615 37.342804895203265 18.42664228442819 20.846813897875933 7 22.376564917709945 31.364467576311718 21.463637642425095 24.795329863553242 8 20.37980025320017 35.42833028555352 21.30960753973836 22.882261921507947 9 21.981994654663104 18.832465888310594 21.249120832747224 37.936418624279085 10-11 21.602897735265156 26.119004079336055 27.937121958081306 24.340976227317483 12-13 21.661626107750738 24.188704459136307 26.833239555493037 27.316429877619917 14-15 19.937051624701084 25.82923055282037 24.268532845688565 29.96518497678998 16-17 21.34582923055282 28.192432128288086 25.082290054860035 25.37944858629906 18-19 20.542270361513573 27.16134477422985 26.185820790547194 26.11056407370938 20-21 19.629343086228722 27.829160219440148 26.35426923617949 26.18722745815164 22-23 21.55296103530736 27.65754677169785 25.9769306512871 24.812561541707694 24-25 20.78281052187368 28.924250949500635 25.60908707272472 24.68385145590097 26-27 19.766493177662117 29.11731607821072 26.721761147840766 24.3944295962864 28-29 20.570052046701363 28.03101702067801 28.054578703052467 23.344352229568152 30-31 21.019130679420453 27.604796736531156 27.60796173864116 23.768110845407232 32-33 19.182726121817414 29.38387958925306 27.34491489660993 24.088479392319595 34-35 20.309818539879025 28.703404135602756 27.20108313405542 23.785694190462795 36-37 20.915037276691518 27.971585314390207 26.98691799127866 24.126459417639612 38-39 20.38261358840906 27.67372344914897 28.077788718525813 23.865874243916164 40-41 20.087565058376704 27.01153467435645 28.08552539035026 24.815374876916586 42-43 19.321282880855254 26.882824588549724 28.77127584751723 25.024616683077788 44-45 18.954845969897313 27.44795329863553 28.825080883387255 24.7721198480799 46-47 19.444014629343087 27.739133492755663 28.420663947109297 24.396187930791953 48-49 18.99282599521733 28.062315374876917 28.39112392741595 24.553734702489802 50-51 18.86200590800394 27.9149669433113 27.766211844141232 25.456815304543532 52-53 18.46040230693487 27.598818399212266 28.16992544661697 25.770853847235898 54-55 18.291953861302574 27.77219018146012 27.834083556055706 26.1017724011816 56-57 18.591222394148264 27.695526797017866 27.92903361935575 25.784217189478127 58-59 19.06702771135181 27.591433394288927 27.15536643691096 26.186172457448304 60-61 18.674919116612745 27.004853003235336 27.366718244478825 26.95350963567309 62-63 18.82930088620059 27.023843015895345 27.52250668167112 26.624349416232945 64-65 19.09551273034182 27.628006752004502 26.44359262906175 26.832887888591927 66-67 18.995002795761696 28.236138121177806 25.855696496330342 26.913162586730156 68-69 18.749824166549445 28.419960613307076 26.275144183429454 26.555071036714022 70-71 19.003024335349558 28.841257560838372 26.16261077507385 25.99310732873822 72-73 19.159867773245182 28.43262062174708 25.998030665353777 26.40948093965396 74-75 19.016739344492898 28.73962582641722 25.84048389365593 26.403150935433956 76 18.71571247714165 27.99127866085244 26.10564073709383 27.18736812491208 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 0.5 11 1.5 12 3.0 13 4.0 14 6.5 15 14.0 16 30.5 17 41.0 18 65.0 19 88.5 20 117.5 21 147.0 22 202.0 23 293.0 24 394.0 25 459.0 26 570.5 27 821.5 28 1096.5 29 1232.0 30 1442.0 31 1819.5 32 2326.0 33 2665.0 34 2984.5 35 3673.5 36 4348.5 37 4654.0 38 5150.5 39 6156.5 40 7345.5 41 8209.0 42 8393.0 43 8685.5 44 9333.0 45 9670.5 46 9653.0 47 9602.0 48 9242.0 49 8565.5 50 8198.0 51 7604.0 52 6460.0 53 5476.5 54 5043.0 55 4697.5 56 3863.5 57 3075.0 58 2775.0 59 2352.5 60 1684.5 61 1276.5 62 1114.0 63 970.5 64 711.5 65 505.5 66 349.5 67 284.0 68 237.0 69 171.0 70 131.0 71 110.0 72 86.0 73 50.5 74 40.0 75 41.0 76 30.0 77 20.0 78 22.0 79 23.0 80 17.0 81 11.0 82 10.0 83 9.0 84 9.0 85 7.0 86 5.0 87 5.0 88 5.0 89 5.0 90 3.5 91 3.0 92 4.0 93 4.5 94 4.5 95 2.0 96 0.0 97 2.0 98 3.5 99 7.5 100 12.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.737797158531439 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 3.516669011112674E-4 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 142180.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.7181038120692 #Duplication Level Percentage of deduplicated Percentage of total 1 80.44050600398126 52.86397524265016 2 9.766904257368521 12.837248558165706 3 4.200646417945589 8.281755521170348 4 1.8461439671225839 4.85300323533549 5 1.0445429054560242 3.43226895484597 6 0.6410668036559002 2.52778168518779 7 0.46233866307069926 2.1268814179209454 8 0.3702990218112545 1.9468279645519764 9 0.24615252894967785 1.4559009706006472 >10 0.9792589738650228 9.59769306512871 >50 0.0021404567734754597 0.0766633844422563 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.004220002813335209 0.0 2 0.0 0.0 0.0 0.004220002813335209 0.0 3 0.0 0.0 0.0 0.004923336615557744 0.0 4 0.0 0.0 0.0 0.006330004220002813 0.0 5 0.0 0.0 0.0 0.008440005626670417 0.0 6 0.0 0.0 0.0 0.008440005626670417 0.0 7 0.0 0.0 0.0 0.008440005626670417 0.0 8 0.0 0.0 0.0 0.009846673231115488 0.0 9 0.0 0.0 0.0 0.012660008440005626 0.0 10 0.0 0.0 0.0 0.014066676044450697 0.0 11 0.0 0.0 0.0 0.01477000984667323 0.0 12 0.0 0.0 0.0 0.01477000984667323 0.0 13 0.0 0.0 0.0 0.01477000984667323 0.0 14 0.0 0.0 0.0 0.0161766774511183 0.0 15 0.0 0.0 0.0 0.016880011253340835 0.0 16 0.0 0.0 0.0 0.01758334505556337 0.0 17 0.0 0.0 0.0 0.018286678857785905 0.0 18 0.0 0.0 0.0 0.021100014066676043 0.0 19 0.0 0.0 0.0 0.02180334786889858 0.0 20 0.0 0.0 0.0 0.02461668307778872 0.0 21 0.0 0.0 0.0 0.028133352088901394 0.0 22 0.0 0.0 0.0 0.0323533549022366 0.0 23 0.0 0.0 0.0 0.03305668870445914 0.0 24 0.0 0.0 0.0 0.03727669151779434 0.0 25 0.0 0.0 0.0 0.03798002532001688 0.0 26 0.0 0.0 0.0 0.040090026726684484 0.0 27 0.0 0.0 0.0 0.05626670417780279 0.0 28 0.0 0.0 0.0 0.07244338162892108 0.0 29 0.0 0.0 0.0 0.09213672809115206 0.0 30 0.0 0.0 0.0 0.11675341116894078 0.0 31 0.0 0.0 0.0 0.15473343648895765 0.0 32 0.0 0.0 0.0 0.18708679139119427 0.0 33 0.0 0.0 0.0 0.24194682796455197 0.0 34 0.0 0.0 0.0 0.3108735405823604 0.0 35 0.0 0.0 0.0 0.37558025038683357 0.0 36 0.0 0.0 0.0 0.47193698129132083 0.0 37 0.0 0.0 0.0 0.6526937684625123 0.0 38 0.0 0.0 0.0 0.9656773104515403 0.0 39 0.0 0.0 0.0 1.3482908988605993 0.0 40 0.0 0.0 0.0 1.7351244900829934 0.0 41 0.0 0.0 0.0 2.1627514418342946 0.0 42 0.0 0.0 0.0 2.594598396398931 0.0 43 0.0 0.0 0.0 3.034885356590238 0.0 44 0.0 0.0 0.0 3.4765789843859896 0.0 45 0.0 0.0 0.0 3.9140526093684063 0.0 46 0.0 0.0 0.0 4.3473062315374875 0.0 47 0.0 0.0 0.0 4.809396539597693 0.0 48 0.0 0.0 0.0 5.253903502602335 0.0 49 0.0 0.0 0.0 5.721620481080321 0.0 50 0.0 0.0 0.0 6.2027008018005345 0.0 51 0.0 0.0 0.0 6.701364467576312 0.0 52 0.0 0.0 0.0 7.1634547756365174 0.0 53 0.0 0.0 0.0 7.6065550710367145 0.0 54 0.0 0.0 0.0 8.001125334083556 0.0 55 0.0 0.0 0.0 8.44070896047264 0.0 56 0.0 0.0 0.0 8.825432550288367 0.0 57 0.0 0.0 0.0 9.251652834435223 0.0 58 0.0 0.0 0.0 9.687016458010971 0.0 59 0.0 0.0 0.0 10.05556337037558 0.0 60 0.0 0.0 0.0 10.49374032916022 0.0 61 0.0 0.0 0.0 10.938247292164862 0.0 62 0.0 0.0 0.0 11.356027570685047 0.0 63 0.0 0.0 0.0 11.816711211140808 0.0 64 0.0 0.0 0.0 12.190181460120973 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGTGC 15 0.00222177 69.992966 8 TTAAACG 15 0.00222177 69.992966 52 AGATACG 20 0.0069423313 52.494724 44 GCCGGAA 20 0.0069423313 52.494724 66 AGCTACG 20 0.0069423313 52.494724 53 TCGCCAG 35 2.0794034E-5 49.994976 48 CGCCAGA 45 1.6764698E-6 46.661976 49 GTTTGCG 35 0.0012526795 39.99598 12 GCGGTTT 35 0.0012526795 39.99598 16 TGCGGTT 35 0.0012526795 39.99598 15 TTTGCGG 35 0.0012526795 39.99598 13 TTCGATG 35 0.0012526795 39.99598 21 CTCTAGC 45 9.166692E-5 38.88498 2 TTAGCCC 50 1.7039744E-4 34.996483 10 TCGATGA 40 0.0024135213 34.996483 22 GCTCGCC 50 1.7039744E-4 34.996483 46 CTCGCCA 55 2.9821272E-4 31.814983 47 TTTCGAT 45 0.004298007 31.107983 20 CACGATT 45 0.004298007 31.107983 25 AACGCCT 45 0.004298007 31.107983 55 >>END_MODULE