FastQCFastQC Report
Wed 25 May 2016
SRR1780458_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780458_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142180
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGT42052.957518638345759No Hit
ATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTCTT35892.5242650161766775No Hit
CTTTACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTC12480.8777605851737234No Hit
CTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTC5470.3847235898157266No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCG4080.2869601913067942No Hit
TATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTCT2640.1856801237867492No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCC2450.17231678154452104No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATATCGTATGCCGT2050.14418342945561963No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCC1920.13504009002672668No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGTTGGGAACTCGTATGCCGT1560.10972007314671542No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGTTGGGACCTCGTATGCCGT1450.10198340132226755No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGAC150.002220884770.0000132
CCCCCGG150.002220884770.000016
TGTAGCG150.002220884770.0000116
CGGTCGT150.002220884770.0000110
CCCGGTC150.002220884770.000018
CTATACA1050.056.666671
GGCGTTG252.3838911E-455.99999650
CCCCGGT200.00693957452.57
GTCCGGG200.00693957452.545
ACGTCCG200.00693957452.543
CCGGTCG200.00693957452.59
CCAACCG200.00693957452.513
CGTCCGG200.00693957452.544
GTTGGAC200.00693957452.545
CGCCAAC200.00693957452.548
TTATCAA352.0781674E-550.00000432
TCCGCGC305.861937E-446.66666813
CTCCGCG305.861937E-446.66666812
GCGCCCA305.861937E-446.66666816
GTTATAG305.861937E-446.6666682
Produced by FastQC (version 0.11.5)