##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780458_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 142180 Sequences flagged as poor quality 0 Sequence length 76 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.10500773667182 34.0 34.0 34.0 32.0 34.0 2 33.411281474187646 34.0 34.0 34.0 33.0 34.0 3 33.55810943873963 34.0 34.0 34.0 34.0 34.0 4 33.570185680123785 34.0 34.0 34.0 34.0 34.0 5 33.57789421859614 34.0 34.0 34.0 34.0 34.0 6 37.34973976649318 38.0 38.0 38.0 37.0 38.0 7 36.98373188915459 38.0 37.0 38.0 36.0 38.0 8 37.02614995076664 38.0 37.0 38.0 36.0 38.0 9 36.83439302292868 38.0 37.0 38.0 35.0 38.0 10-11 36.55333028555353 38.0 37.0 38.0 34.0 38.0 12-13 36.422415248276835 38.0 37.0 38.0 34.0 38.0 14-15 36.631875087916725 38.0 37.0 38.0 34.0 38.0 16-17 36.724212266141514 38.0 37.0 38.0 34.5 38.0 18-19 36.75086158390772 38.0 37.0 38.0 34.0 38.0 20-21 36.501540301026864 38.0 37.0 38.0 34.0 38.0 22-23 36.5691447460965 38.0 37.0 38.0 34.0 38.0 24-25 35.908506822337884 38.0 36.5 38.0 32.5 38.0 26-27 36.09756998171332 38.0 37.0 38.0 33.5 38.0 28-29 35.93136868757912 38.0 37.0 38.0 32.0 38.0 30-31 35.69298072865382 38.0 36.5 38.0 31.5 38.0 32-33 36.11466802644535 38.0 37.0 38.0 34.0 38.0 34-35 36.031970037980024 38.0 37.0 38.0 33.0 38.0 36-37 36.11272682515121 38.0 37.0 38.0 34.0 38.0 38-39 35.63995639330426 38.0 36.5 38.0 32.5 38.0 40-41 35.50324588549726 38.0 36.0 38.0 31.0 38.0 42-43 35.99543536362357 38.0 36.5 38.0 32.5 38.0 44-45 36.185402306934876 38.0 37.0 38.0 34.0 38.0 46-47 36.429786186524126 38.0 37.0 38.0 34.0 38.0 48-49 36.21409480939654 38.0 37.0 38.0 34.0 38.0 50-51 36.33079898719932 38.0 37.0 38.0 34.0 38.0 52-53 36.23228302152201 38.0 37.0 38.0 34.0 38.0 54-55 36.38795892530595 38.0 37.0 38.0 34.0 38.0 56-57 36.42759881839922 38.0 37.0 38.0 34.0 38.0 58-59 36.45821493880996 38.0 37.0 38.0 34.0 38.0 60-61 36.20942818961879 38.0 37.0 38.0 33.5 38.0 62-63 36.48831762554508 38.0 37.0 38.0 34.0 38.0 64-65 36.48154803769869 38.0 37.0 38.0 34.0 38.0 66-67 35.78578210718807 38.0 37.0 38.0 31.5 38.0 68-69 34.85977985651991 38.0 35.5 38.0 26.0 38.0 70-71 34.446690814460545 38.0 35.5 38.0 24.0 38.0 72-73 33.85757842171895 38.0 34.0 38.0 22.0 38.0 74-75 34.28635532423688 38.0 35.5 38.0 23.0 38.0 76 33.85099170066113 37.0 34.0 38.0 22.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2956686881184467 1101 2 0.37511412519958753 1101 3 0.04586489156866236 1101 4 0.1515761862649896 1101 5 0.16502265061147625 1101 6 -0.7146200110110499 1101 7 0.44954281520909234 1101 8 0.5695767659445394 1101 9 0.24278747296469305 1101 10-11 0.046868278104852834 1101 12-13 0.6808324645251531 1101 14-15 0.5717835677481133 1101 16-17 -0.12205191380724756 1101 18-19 -0.336951591140803 1101 20-21 0.224505180454031 1101 22-23 0.529774113908509 1101 24-25 -1.201289050672166 1101 26-27 -0.09041841614753565 1101 28-29 0.6985289520631568 1101 30-31 0.9964209910217505 1101 32-33 0.18489055059139758 1101 34-35 0.14263618484709895 1101 36-37 0.2891800477145665 1101 38-39 0.23855064656264346 1101 40-41 1.240081613790501 1101 42-43 0.7093916676901273 1101 44-45 0.5742625386382088 1101 46-47 0.6191820300860158 1101 48-49 -1.3015897737057998 1101 50-51 -1.555732624860589 1101 52-53 -1.486718683095539 1101 54-55 -1.1274573751227877 1101 56-57 -0.9233379680499638 1101 58-59 -0.9413585978231609 1101 60-61 -1.2747533266740518 1101 62-63 -0.47387001664621664 1101 64-65 -0.9761303603860867 1101 66-67 -1.692114475229701 1101 68-69 -0.8976957607389693 1101 70-71 -0.9386664515164185 1101 72-73 -1.6849211842000642 1101 74-75 -0.6982051679918868 1101 76 -0.9570899714874699 1102 1 -0.5468470351519912 1102 2 0.19382032466050703 1102 3 0.18408591582741707 1102 4 0.3626094297510676 1102 5 0.1066039623814703 1102 6 0.18072590093863283 1102 7 0.020450272531640223 1102 8 0.40010686477652513 1102 9 0.42073895544447737 1102 10-11 0.41766791871582143 1102 12-13 0.6910391132223737 1102 14-15 -0.2433197566004921 1102 16-17 0.22331196759436978 1102 18-19 -0.08547809608240442 1102 20-21 0.6512527096543863 1102 22-23 0.5035836377180374 1102 24-25 -4.832807469360397 1102 26-27 -1.110544202311054 1102 28-29 -5.366364363936782E-4 1102 30-31 1.5160346478052205 1102 32-33 0.16930205104063134 1102 34-35 0.4161132737958866 1102 36-37 -2.349885540784989 1102 38-39 0.027355678009179485 1102 40-41 1.0019684062433356 1102 42-43 0.6908561780225568 1102 44-45 0.6909831136606712 1102 46-47 1.0437193166987697 1102 48-49 -2.9383732418100195 1102 50-51 -0.5538727865856572 1102 52-53 -0.19984536773166184 1102 54-55 0.6305390309868244 1102 56-57 0.5283781146095095 1102 58-59 0.08721283074826403 1102 60-61 -5.305409211669559 1102 62-63 -2.0994405467450505 1102 64-65 0.041039450934668764 1102 66-67 1.1833841770613986 1102 68-69 -0.8388008820327713 1102 70-71 -1.1829701352540596 1102 72-73 -5.291560896688836 1102 74-75 -1.8734073243261165 1102 76 -0.0633074018558375 1103 1 0.4787609052946564 1103 2 0.4471914696445509 1103 3 0.040597791048973875 1103 4 0.3009433538914408 1103 5 0.2214001339518319 1103 6 0.36777304734062 1103 7 0.6504963554809464 1103 8 0.5580437665843903 1103 9 0.6194202741257939 1103 10-11 0.4710532430688801 1103 12-13 -0.3793685436369003 1103 14-15 -0.23062213164480738 1103 16-17 0.2845916556482493 1103 18-19 0.6287615352540072 1103 20-21 -0.10751723008329606 1103 22-23 0.48242092945855575 1103 24-25 -2.9664622017248092 1103 26-27 -0.4997501576460408 1103 28-29 -0.07009707599683423 1103 30-31 1.084588352165376 1103 32-33 1.0030312250923927 1103 34-35 0.29267832165125895 1103 36-37 -0.9354013153330172 1103 38-39 -0.19693712596103552 1103 40-41 -0.10319288407924176 1103 42-43 -0.1129509825587931 1103 44-45 1.0685194482937774 1103 46-47 0.7976470019167792 1103 48-49 -2.0088872439369254 1103 50-51 -0.3692538571280153 1103 52-53 0.198531094515765 1103 54-55 -0.0586846485594279 1103 56-57 0.4334047009264168 1103 58-59 -0.2862717491879252 1103 60-61 -4.093147602311177 1103 62-63 -1.098848558088548 1103 64-65 0.2956052077584914 1103 66-67 -0.30210323874859313 1103 68-69 0.4797563671696423 1103 70-71 0.4604080994144937 1103 72-73 -4.3441202727966015 1103 74-75 -1.9967678347834052 1103 76 -2.9145161930646317 1104 1 0.13273629818134225 1104 2 0.2437756818033634 1104 3 0.30244901106551225 1104 4 -0.19389354643941203 1104 5 -0.5494525852375816 1104 6 0.05999673427196939 1104 7 0.420405629674498 1104 8 0.5024580552527169 1104 9 0.7386109792539983 1104 10-11 -0.7021981527127537 1104 12-13 0.2143426846509442 1104 14-15 0.5953930410185464 1104 16-17 0.10067803902293804 1104 18-19 0.619268927727127 1104 20-21 -0.3878916355837134 1104 22-23 0.13524286390850904 1104 24-25 0.5216865782586453 1104 26-27 0.9056016310222788 1104 28-29 1.2308844945159905 1104 30-31 0.6132891120909321 1104 32-33 0.5807975867549189 1104 34-35 -0.15136440477554203 1104 36-37 0.9061710068340574 1104 38-39 1.0588140113425126 1104 40-41 1.2021246562433348 1104 42-43 0.23973266611779565 1104 44-45 0.5891750779463862 1104 46-47 0.697454435746387 1104 48-49 -0.10495062276240219 1104 50-51 0.22763018960481674 1104 52-53 0.20173945369690927 1104 54-55 0.13838873336778335 1104 56-57 0.3897025193714114 1104 58-59 -0.5194835978231609 1104 60-61 0.2649479311875851 1104 62-63 0.4118168937311353 1104 64-65 0.5567165342679985 1104 66-67 -0.22311879912906818 1104 68-69 1.3519357251100885 1104 70-71 0.8163453409364081 1104 72-73 0.8036548771206924 1104 74-75 -0.08583291956421846 1104 76 -0.12925978280821937 1105 1 0.7075094004915741 1105 2 0.31145823130831474 1105 3 0.3138454632107326 1105 4 -0.1615348418189484 1105 5 0.01647666393733971 1105 6 0.2719140197505183 1105 7 0.6836327336348944 1105 8 -0.2874037434271557 1105 9 0.5984758719932728 1105 10-11 0.5601007334258554 1105 12-13 -0.06273033515103776 1105 14-15 0.45284714580403573 1105 16-17 0.20162172714174886 1105 18-19 0.44339743432778533 1105 20-21 0.36188920187503726 1105 22-23 0.39322295879300384 1105 24-25 0.5152406211629383 1105 26-27 0.6882181739265647 1105 28-29 1.033006503591892 1105 30-31 1.0604193719919195 1105 32-33 0.8827391090321441 1105 34-35 -0.41602251121118883 1105 36-37 0.6397467081541919 1105 38-39 0.09908835127651372 1105 40-41 0.38479018842155455 1105 42-43 0.22154217519370434 1105 44-45 0.6558133824018313 1105 46-47 0.8762652855813755 1105 48-49 0.08352916271614674 1105 50-51 0.5875982176576287 1105 52-53 0.3113851838949273 1105 54-55 0.6397681681862579 1105 56-57 0.2468858939588756 1105 58-59 0.3321562536619851 1105 60-61 0.8163093173261942 1105 62-63 -0.4104165014833825 1105 64-65 0.6118810350930843 1105 66-67 0.40453693519435774 1105 68-69 -0.10269762885030076 1105 70-71 0.711817700672384 1105 72-73 0.6609284951074841 1105 74-75 -0.46163221824408396 1105 76 -0.09749410624056765 1106 1 -0.8812202942049154 1106 2 0.18199382247188822 1106 3 0.09282447531806781 1106 4 0.1338469944148173 1106 5 -0.11921378533043026 1106 6 -0.08072256702794078 1106 7 -0.9150531098798211 1106 8 0.32872604503916136 1106 9 -0.9173271010616943 1106 10-11 0.11894129714796975 1106 12-13 0.9978994395255967 1106 14-15 -0.469758276298073 1106 16-17 0.11292995406472528 1106 18-19 0.3475143432331578 1106 20-21 0.07585676854813528 1106 22-23 0.3043794992381308 1106 24-25 -0.41423206148136416 1106 26-27 0.8294279425357374 1106 28-29 0.43472400821143253 1106 30-31 0.5445543453778399 1106 32-33 -0.14651569667126552 1106 34-35 0.5026114831074651 1106 36-37 1.0971021233614522 1106 38-39 0.429389104193028 1106 40-41 0.8553388797809376 1106 42-43 0.7353766714567058 1106 44-45 0.897942922653904 1106 46-47 0.7168152180119449 1106 48-49 1.2033856121493898 1106 50-51 -0.13388472450845512 1106 52-53 0.2874049307164128 1106 54-55 0.12739537798244527 1106 56-57 0.2915058503834871 1106 58-59 0.4890035191684845 1106 60-61 0.9193699367586206 1106 62-63 0.5987329510663315 1106 64-65 -1.112804560207394 1106 66-67 0.31980280022096963 1106 68-69 1.1780912787312587 1106 70-71 0.5605276761267532 1106 72-73 0.8571418490334111 1106 74-75 0.38047250058435367 1106 76 1.0482007557247428 1107 1 0.6510222142426798 1107 2 -0.3793515881724261 1107 3 0.07430383350296665 1107 4 0.18372323120384948 1107 5 0.11996957981488521 1107 6 0.11045955711296784 1107 7 0.47827109577619353 1107 8 0.09652332966756205 1107 9 -0.2222634658630298 1107 10-11 0.3433337782799839 1107 12-13 0.28338778150324373 1107 14-15 0.36433157511863357 1107 16-17 0.5383482872069578 1107 18-19 0.7150303784938714 1107 20-21 0.8292914505744875 1107 22-23 -0.2339466286257874 1107 24-25 0.2165387776129748 1107 26-27 -0.05570158729894814 1107 28-29 0.6660735330551262 1107 30-31 0.7961072870304022 1107 32-33 1.0636119784014042 1107 34-35 0.09497525926803974 1107 36-37 1.0273057425079912 1107 38-39 0.7327067675975627 1107 40-41 0.8543170745242108 1107 42-43 0.8031564201533072 1107 44-45 0.79652790784953 1107 46-47 0.8766248857060361 1107 48-49 0.8031279688437394 1107 50-51 1.047652637143159 1107 52-53 0.8279028162877111 1107 54-55 0.7615317670643051 1107 56-57 0.8497824729630281 1107 58-59 0.7414501188838614 1107 60-61 1.226963669686377 1107 62-63 0.7746756760152422 1107 64-65 0.8688602741792195 1107 66-67 0.11151977028174542 1107 68-69 1.0630150985967717 1107 70-71 -0.36599676963663086 1107 72-73 0.9134996359988179 1107 74-75 -0.3431409805004577 1107 76 0.04376281606183596 1108 1 -0.5114271878237489 1108 2 0.2699962606837687 1108 3 0.14512553778307336 1108 4 0.35288205788629057 1108 5 0.11425209033493644 1108 6 -0.3312842771784119 1108 7 -0.020931043353492385 1108 8 -0.4353202526353286 1108 9 -0.015146177234548475 1108 10-11 0.43987802413202814 1108 12-13 0.8567170485186324 1108 14-15 0.3642484004333042 1108 16-17 -0.19709152204576696 1108 18-19 0.36252990100956595 1108 20-21 0.9525286093272314 1108 22-23 0.15636444469732425 1108 24-25 1.0498751914902016 1108 26-27 -0.12034063997008815 1108 28-29 -0.48622547685805984 1108 30-31 0.31650356819780257 1108 32-33 0.8324972527854513 1108 34-35 0.42156220145854917 1108 36-37 1.1623136595058128 1108 38-39 1.1785261614697404 1108 40-41 0.9440040296530086 1108 42-43 -0.22453458897125955 1108 44-45 0.48285152514740304 1108 46-47 0.7655404726420656 1108 48-49 1.06016547138519 1108 50-51 0.607381302836373 1108 52-53 -0.10663562581962793 1108 54-55 -0.49570597083069146 1108 56-57 0.28718582089813083 1108 58-59 0.3945192647722564 1108 60-61 0.8554377530705395 1108 62-63 0.7503486208303727 1108 64-65 0.5926679496624061 1108 66-67 0.7810359400566753 1108 68-69 1.4546015170948223 1108 70-71 -0.036270753211169904 1108 72-73 0.2897295431512248 1108 74-75 -0.21673702261764305 1108 76 0.7883610823317255 1109 1 -0.7340510653786936 1109 2 0.11474151933484933 1109 3 0.14802258369356025 1109 4 0.19778755137755155 1109 5 0.044408928196510544 1109 6 -0.47010533331829407 1109 7 -0.4082506990408703 1109 8 0.4682824675113224 1109 9 0.7347080090608884 1109 10-11 0.3505214631562623 1109 12-13 0.30569186601114495 1109 14-15 0.6264823304811884 1109 16-17 0.6929521259247053 1109 18-19 0.5968284890201545 1109 20-21 0.8802883339796779 1109 22-23 0.42628964707392214 1109 24-25 -0.17212377018802982 1109 26-27 0.23794446267634584 1109 28-29 -0.7185179246695697 1109 30-31 0.4928644426954705 1109 32-33 0.7337317019942162 1109 34-35 -0.14714329331458487 1109 36-37 0.8589089896216393 1109 38-39 0.6341314966489833 1109 40-41 0.34423540876222347 1109 42-43 0.8812268149423161 1109 44-45 0.655168072278876 1109 46-47 0.6463353656372419 1109 48-49 0.40668613206547377 1109 50-51 -0.15114158831288904 1109 52-53 -0.48801377363306386 1109 54-55 -0.5991119225936004 1109 56-57 0.004239935408357098 1109 58-59 -0.26251728799947927 1109 60-61 0.7027627926485422 1109 62-63 0.26195923734994864 1109 64-65 0.7385457481638937 1109 66-67 0.20823484276849058 1109 68-69 -0.5797688561241685 1109 70-71 0.013514211633307127 1109 72-73 -0.5034104828709189 1109 74-75 -0.3863616244340733 1109 76 -0.03910445115415229 1110 1 -0.7666408495849808 1110 2 -0.5050161996399325 1110 3 -0.03126018137434983 1110 4 0.13758733844031212 1110 5 0.31633886665245825 1110 6 0.09175682877368274 1110 7 0.4216137482312021 1110 8 0.6441569384142269 1110 9 0.7197374826904266 1110 10-11 -0.810203951681828 1110 12-13 0.30793160217672266 1110 14-15 0.6345226587161434 1110 16-17 0.2982456936621105 1110 18-19 -0.1681290533725317 1110 20-21 0.6499493223200687 1110 22-23 -0.7017550957134802 1110 24-25 -1.209037218992215 1110 26-27 0.8788887873796654 1110 28-29 0.39431608815859676 1110 30-31 -0.1719476791461787 1110 32-33 0.6021812852085233 1110 34-35 0.6463562782141494 1110 36-37 1.1563589363873277 1110 38-39 0.6885927914112457 1110 40-41 -0.8858666453030608 1110 42-43 0.2465115535748481 1110 44-45 0.8365574877402011 1110 46-47 0.3558253549903796 1110 48-49 0.8840420748321094 1110 50-51 -0.12446925197593117 1110 52-53 0.49519145111958807 1110 54-55 -0.1736951369071349 1110 56-57 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2116 32-33 -0.006983663245080152 2116 34-35 -1.0757914881088766 2116 36-37 0.6484001735007325 2116 38-39 -4.117310988657479 2116 40-41 -0.9166982604233382 2116 42-43 -1.3430485838822008 2116 44-45 -0.43273117205361444 2116 46-47 -0.4472330642536093 2116 48-49 -0.25799228942906893 2116 50-51 -0.8570156437285092 2116 52-53 0.12891653703024275 2116 54-55 -0.3224133499655508 2116 56-57 -0.24152664729525242 2116 58-59 -2.3200252644898285 2116 60-61 -1.4432187354790855 2116 62-63 0.41036897706447206 2116 64-65 -1.6649605490653343 2116 66-67 -0.5381396324624035 2116 68-69 -1.1529913582232467 2116 70-71 0.6378870076030836 2116 72-73 0.2129152937873542 2116 74-75 0.0953545804357887 2116 76 -0.6402597828082222 2117 1 0.6305534953519825 2117 2 0.44120190859381125 2117 3 0.19127803405402233 2117 4 -0.04953153273472566 2117 5 0.11136334093394851 2117 6 0.2667205958988461 2117 7 0.6105374267567285 2117 8 0.36064494738356956 2117 9 -0.6033913969617402 2117 10-11 -0.2924874431636795 2117 12-13 -0.928354596516165 2117 14-15 0.21722590463482305 2117 16-17 -1.0708032275553094 2117 18-19 -0.9572742309819873 2117 20-21 -0.12646991255099493 2117 22-23 0.01540629781656122 2117 24-25 -1.322712736507043 2117 26-27 -1.9875999052288265 2117 28-29 -0.731900789524687 2117 30-31 -0.0908202214634457 2117 32-33 -1.332384193749057 2117 34-35 0.5106983438981985 2117 36-37 -0.6379340440059025 2117 38-39 0.2328994445874386 2117 40-41 0.16398432136269747 2117 42-43 0.48780199083044096 2117 44-45 -0.3716277237777561 2117 46-47 -0.6103276707964866 2117 48-49 -0.7296881338145624 2117 50-51 -0.1564533095110079 2117 52-53 0.5873391718666667 2117 54-55 -0.0150464180469001 2117 56-57 0.2177377205208444 2117 58-59 -0.8498466614836389 2117 60-61 -0.6155228910935833 2117 62-63 -0.4272048514059108 2117 64-65 -0.772461875325277 2117 66-67 -0.5184119578381825 2117 68-69 0.24393879208621883 2117 70-71 -0.032447209903551766 2117 72-73 0.9648127296847946 2117 74-75 0.6489496290653207 2117 76 0.47540423133855114 2118 1 0.05212732382236851 2118 2 0.3648093356495181 2118 3 -0.6263170145755126 2118 4 0.2879574150990507 2118 5 0.24257465835216152 2118 6 0.28944384965658543 2118 7 0.7324649245462922 2118 8 0.5998138244834834 2118 9 -1.434425879720358 2118 10-11 -0.5165811655332675 2118 12-13 -0.6690707768875157 2118 14-15 0.5281454448647054 2118 16-17 -2.6326953584129598 2118 18-19 -0.34807882868313555 2118 20-21 -0.322446924045245 2118 22-23 0.6977228318572273 2118 24-25 0.7350679885150626 2118 26-27 -2.1284929202597738 2118 28-29 -0.24702015291990875 2118 30-31 -0.5040386122680403 2118 32-33 0.1652727470113291 2118 34-35 -0.29938123169861797 2118 36-37 -2.3435485444479838 2118 38-39 -0.17956739891389617 2118 40-41 -0.382390568115639 2118 42-43 0.6094642366306147 2118 44-45 -1.633961941284383 2118 46-47 0.3991771921566496 2118 48-49 0.2940077105709378 2118 50-51 -0.6266566693695381 2118 52-53 -0.9366219245082235 2118 54-55 -0.5247723243245304 2118 56-57 0.47083232706372513 2118 58-59 -0.9772560337206002 2118 60-61 -0.7917828380431828 2118 62-63 -0.8171694844739861 2118 64-65 0.20180868170390198 2118 66-67 0.2882193418965713 2118 68-69 -0.6867862300181145 2118 70-71 -0.19575401803794534 2118 72-73 0.695581960454021 2118 74-75 -0.07761977853856905 2118 76 -0.12477260332104123 2119 1 0.42721291035209674 2119 2 -0.716012295035874 2119 3 0.021213202709475354 2119 4 -0.23234580574912655 2119 5 -0.09084251484702577 2119 6 -0.4382931726308499 2119 7 -0.5455131373554778 2119 8 0.10079195625180404 2119 9 -0.16909366425190342 2119 10-11 0.022533625216397013 2119 12-13 2.333522258766152E-4 2119 14-15 -0.05045093604775275 2119 16-17 -0.11264365716235147 2119 18-19 -0.028090007331918798 2119 20-21 -1.3215421521595516 2119 22-23 0.10992851899479916 2119 24-25 0.8894346481950706 2119 26-27 0.35907432629930014 2119 28-29 0.21726406422533984 2119 30-31 0.8789034683579686 2119 32-33 0.23607900705464857 2119 34-35 0.5579233166141009 2119 36-37 0.04292878385494703 2119 38-39 -1.3260775644976306 2119 40-41 -0.48936674362042965 2119 42-43 0.1748733053094469 2119 44-45 0.7140154219518351 2119 46-47 0.29512842530134265 2119 48-49 -0.13451181713115545 2119 50-51 0.7044339475521397 2119 52-53 0.31065677317919693 2119 54-55 0.29064023768203384 2119 56-57 -0.654525739030035 2119 58-59 -0.0026664997305161364 2119 60-61 0.37785665053363715 2119 62-63 0.056312664621238184 2119 64-65 -0.08291150274380499 2119 66-67 0.6437422930598444 2119 68-69 1.2092139097149968 2119 70-71 0.8841104408455891 2119 72-73 1.1533476280289534 2119 74-75 0.7606915468299675 2119 76 0.7046412162835125 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 2.0 22 11.0 23 34.0 24 60.0 25 124.0 26 271.0 27 451.0 28 912.0 29 1444.0 30 2373.0 31 3642.0 32 5600.0 33 8365.0 34 12641.0 35 17788.0 36 29063.0 37 59399.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.0524687016458 21.98832465888311 10.836263890842595 29.1229427486285 2 18.194542129694753 20.113236742157827 40.203263468842316 21.488957659305104 3 16.868757912505274 25.534533689689127 34.6940497960332 22.9026586017724 4 11.905331270220847 21.77380784920523 38.1523421015614 28.16851877901252 5 11.069770713180475 38.10732873821916 37.80700520467013 13.015895343930229 6 23.560275706850472 40.27641018427345 18.606695737797157 17.556618371078912 7 22.84146856097904 34.32128288085525 21.93346462230975 20.90378393585596 8 20.681530454353634 37.009424672949784 22.348431565621045 19.96061330707554 9 20.559150372766915 18.51596567731045 21.038824025882686 39.886059924039955 10-11 21.364467576311718 25.937543958362642 29.96729497819665 22.730693487128992 12-13 22.88507525671684 21.577226051484033 28.27577718385146 27.261921507947672 14-15 21.896891264594174 24.11415107610072 23.22126881417921 30.767688845125896 16-17 23.22513715009143 28.161837107891408 23.523350682233787 25.089675059783374 18-19 22.125826417217613 27.264383176255453 24.356449570966383 26.253340835560557 20-21 21.02088901392601 26.244549163032776 24.286116190744128 28.448445632297087 22-23 22.26121817414545 29.244267829511887 24.693346462230974 23.801167534111688 24-25 22.93501195667464 30.035166690111126 24.563581375720915 22.46623997749332 26-27 23.762484174989453 30.646715431143623 24.465114643409763 21.12568575045717 28-29 22.440216626811086 28.602475734983823 26.982697988465326 21.974609649739765 30-31 26.729849486566327 25.329160219440144 27.696230130820087 20.244760163173442 32-33 24.62266141510761 25.065058376705586 30.518357012238006 19.7939231959488 34-35 26.330355886903924 26.22204248136165 27.952243634829088 19.495357996905334 36-37 23.270853847235898 27.66704177802785 27.486988324658885 21.575116050077366 38-39 21.2234491489661 25.886200590800396 28.342242228161485 24.548108032072022 40-41 20.252848501899003 25.665002110001407 30.32951188634126 23.752637501758333 42-43 23.170277113518075 25.448375298916865 30.658320438880292 20.723027148684768 44-45 19.731326487550994 26.891616261077505 29.523491348994234 23.85356590237727 46-47 22.881910254606836 24.257279504853003 28.422422281614857 24.438387958925308 48-49 21.782247854831905 23.914052609368404 29.13103108735406 25.172668448445634 50-51 19.01216767477845 23.969264312842874 31.315937543958363 25.702630468420313 52-53 19.44682796455198 24.35574623716416 33.8015192010128 22.395906597271065 54-55 18.559220706147137 24.491489660993107 31.951399634266426 24.99788999859333 56-57 21.870867913911944 23.966802644535097 29.670839780559856 24.491489660993107 58-59 18.960824307216203 27.786608524405683 31.33809255872837 21.91447460964974 60-61 20.258475172316782 31.965114643409763 26.479814319876215 21.296595864397244 62-63 17.606203404135602 35.369601913067946 25.22436348290899 21.799831199887468 64-65 17.725770150513434 36.06555071036714 24.566394710929806 21.64228442818962 66-67 17.414896609931073 35.64108876072584 24.235827823885217 22.70818680545787 68-69 17.495779997186663 34.82275988183992 24.594176396117597 23.087283724855816 70-71 18.234632156421437 33.489238992825996 24.470741313827542 23.805387536925025 72-73 18.723097482064986 31.67639611759741 24.643761429174287 24.956744971163314 74-75 18.993529329019555 31.333169222112815 24.536854691236464 25.13644675763117 76 18.96187930791954 31.260374173582782 24.84737656491771 24.93036995357997 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 1.0 8 1.0 9 1.0 10 0.5 11 1.5 12 3.0 13 3.0 14 7.0 15 20.0 16 29.5 17 30.0 18 41.0 19 77.0 20 125.5 21 149.0 22 197.0 23 289.0 24 404.5 25 476.0 26 624.5 27 871.5 28 1117.0 29 1264.0 30 1470.5 31 1843.0 32 2402.0 33 2795.0 34 3014.5 35 3590.5 36 4201.0 37 4455.0 38 4925.0 39 5904.5 40 7098.0 41 8899.0 42 10016.0 43 11164.5 44 12547.0 45 12125.0 46 11469.0 47 10686.0 48 9216.5 49 7947.5 50 7365.0 51 6740.5 52 5520.0 53 4451.5 54 3979.0 55 3799.0 56 3128.5 57 2352.0 58 2066.0 59 1697.0 60 1138.0 61 822.0 62 696.0 63 594.0 64 407.0 65 272.5 66 173.5 67 124.0 68 100.0 69 63.5 70 42.0 71 33.0 72 20.5 73 4.5 74 1.0 75 1.0 76 3.0 77 2.5 78 0.0 79 0.0 80 0.0 81 1.0 82 1.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 142180.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.074834716556474 #Duplication Level Percentage of deduplicated Percentage of total 1 61.31356357193006 25.797580531720353 2 17.73929323660192 14.92755661837108 3 8.645648757982013 10.91292727528485 4 4.095483267025509 6.892671261780841 5 2.0878606532713717 4.39231959487973 6 1.3406439102671257 3.3844422562948373 7 0.9076928220387149 2.673371782247855 8 0.6803517100732172 2.290054860036573 9 0.5182040052154726 1.962301308200872 >10 2.61943766507305 17.712055141370094 >50 0.02340276152586005 0.6857504571669715 >100 0.021731135702584333 1.6247010831340554 >500 0.001671625823275718 0.3847235898157266 >1k 0.005014877469827154 6.35954423969616 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGT 4205 2.957518638345759 No Hit ATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTCTT 3589 2.5242650161766775 No Hit CTTTACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTC 1248 0.8777605851737234 No Hit CTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTC 547 0.3847235898157266 No Hit TCTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCG 408 0.2869601913067942 No Hit TATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTCT 264 0.1856801237867492 No Hit CTCTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCC 245 0.17231678154452104 No Hit CTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATATCGTATGCCGT 205 0.14418342945561963 No Hit TCTTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCC 192 0.13504009002672668 No Hit CTTATACACATCTCCGAGCCCACGAGACAGGTTGGGAACTCGTATGCCGT 156 0.10972007314671542 No Hit CTTATACACATCTCCGAGCCCACGAGACAGGTTGGGACCTCGTATGCCGT 145 0.10198340132226755 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.004923336615557744 0.0 2 0.0 0.0 0.0 0.004923336615557744 0.0 3 0.0 0.0 0.0 0.006330004220002813 0.0 4 0.0 0.0 0.0 0.008440005626670417 0.0 5 0.0 0.0 0.0 0.010550007033338022 0.0 6 0.0 0.0 0.0 0.010550007033338022 0.0 7 0.0 0.0 0.0 0.010550007033338022 0.0 8 0.0 0.0 0.0 0.012660008440005626 0.0 9 0.0 0.0 0.0 0.016880011253340835 0.0 10 0.0 0.0 0.0 0.018286678857785905 0.0 11 0.0 0.0 0.0 0.019693346462230976 0.0 12 0.0 0.0 0.0 0.019693346462230976 0.0 13 0.0 0.0 0.0 0.019693346462230976 0.0 14 0.0 0.0 0.0 0.021100014066676043 0.0 15 0.0 0.0 0.0 0.02180334786889858 0.0 16 0.0 0.0 0.0 0.02321001547334365 0.0 17 0.0 0.0 0.0 0.023913349275566185 0.0 18 0.0 0.0 0.0 0.027430018286678858 0.0 19 0.0 0.0 0.0 0.02883668589112393 0.0 20 0.0 0.0 0.0 0.03305668870445914 0.0 21 0.0 0.0 0.0 0.03657335771557181 0.0 22 0.0 0.0 0.0 0.040793360528907016 0.0 23 0.0 0.0 0.0 0.04220002813335209 0.0 24 0.0 0.0 0.0 0.04712336474890983 0.0 25 0.0 0.0 0.0 0.0485300323533549 0.0 26 0.0 0.0 0.0 0.05134336756224504 0.0 27 0.0 0.0 0.0 0.07033338022225348 0.0 28 0.0 0.0 0.0 0.08791672527781685 0.0 29 0.0 0.0 0.0 0.10690673793782529 0.0 30 0.0 0.0 0.0 0.13504009002672668 0.0 31 0.0 0.0 0.0 0.17864678576452384 0.0 32 0.0 0.0 0.0 0.21381347587565058 0.0 33 0.0 0.0 0.0 0.272190181460121 0.0 34 0.0 0.0 0.0 0.3376002250668167 0.0 35 0.0 0.0 0.0 0.4065269376846251 0.0 36 0.0 0.0 0.0 0.514137009424673 0.0 37 0.0 0.0 0.0 0.7005204670136447 0.0 38 0.0 0.0 0.0 1.0275706850471233 0.0 39 0.0 0.0 0.0 1.4270642847095232 0.0 40 0.0 0.0 0.0 1.847657898438599 0.0 41 0.0 0.0 0.0 2.2970881980587987 0.0 42 0.0 0.0 0.0 2.7556618371078914 0.0 43 0.0 0.0 0.0 3.228302152201435 0.0 44 0.0 0.0 0.0 3.7114924743283164 0.0 45 0.0 0.0 0.0 4.176396117597411 0.0 46 0.0 0.0 0.0 4.6504431002954005 0.0 47 0.0 0.0 0.0 5.175833450555634 0.0 48 0.0 0.0 0.0 5.654100436066957 0.0 49 0.0 0.0 0.0 6.175270783513856 0.0 50 0.0 0.0 0.0 6.693627795751864 0.0 51 0.0 0.0 0.0 7.246448164298776 0.0 52 0.0 0.0 0.0 7.750738500492334 0.0 53 0.0 0.0 0.0 8.23533549022366 0.0 54 0.0 0.0 0.0 8.671402447601631 0.0 55 0.0 0.0 0.0 9.158109438739626 0.0 56 0.0 0.0 0.0 9.573076382050921 0.0 57 0.0 0.0 0.0 10.04149669433113 0.0 58 0.0 0.0 0.0 10.512730341820228 0.0 59 0.0 0.0 0.0 10.91292727528485 0.0 60 0.0 0.0 0.0 11.380644253762837 0.0 61 0.0 0.0 0.0 11.87297791531861 0.0 62 0.0 0.0 0.0 12.325924883949922 0.0 63 0.0 0.0 0.0 12.82740188493459 0.0 64 0.0 0.0 0.0 13.226895484596989 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTATGAC 15 0.0022208847 70.00001 32 CCCCCGG 15 0.0022208847 70.00001 6 TGTAGCG 15 0.0022208847 70.00001 16 CGGTCGT 15 0.0022208847 70.00001 10 CCCGGTC 15 0.0022208847 70.00001 8 CTATACA 105 0.0 56.66667 1 GGCGTTG 25 2.3838911E-4 55.999996 50 CCCCGGT 20 0.006939574 52.5 7 GTCCGGG 20 0.006939574 52.5 45 ACGTCCG 20 0.006939574 52.5 43 CCGGTCG 20 0.006939574 52.5 9 CCAACCG 20 0.006939574 52.5 13 CGTCCGG 20 0.006939574 52.5 44 GTTGGAC 20 0.006939574 52.5 45 CGCCAAC 20 0.006939574 52.5 48 TTATCAA 35 2.0781674E-5 50.000004 32 TCCGCGC 30 5.861937E-4 46.666668 13 CTCCGCG 30 5.861937E-4 46.666668 12 GCGCCCA 30 5.861937E-4 46.666668 16 GTTATAG 30 5.861937E-4 46.666668 2 >>END_MODULE