FastQCFastQC Report
Wed 25 May 2016
SRR1780457_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780457_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences603411
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACAAT953.092282E-1127.82935717
CTTAGAC508.356881E-526.3896481
CGTATTT450.001408059324.4125865
CGTTAAT551.5876052E-424.01050624
CCCAACG703.1744974E-522.03340521
CTCGCTA602.84435E-422.02974313
CGTGTAT602.8587677E-422.01328335
AGCCTCG703.209286E-522.00232510
TGTAACC500.002598554421.9713293
GTATAGA1700.020.6977631
CCAACGT755.4693417E-520.53209522
AATCGTC755.490342E-520.5218828
CGTCCCA755.4973574E-520.51847631
GCGTATC755.5149307E-520.50997244
ATCGGTT654.9302226E-420.30646722
AACAACG550.004512831619.9755927
TCTCGGG1352.6248017E-919.53492743
CGAAACT808.988932E-519.24724225
CTATACA1605.2750693E-1119.2424531
TATACCG809.069426E-519.2249136
Produced by FastQC (version 0.11.5)