FastQCFastQC Report
Wed 25 May 2016
SRR1780457_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780457_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences603411
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGT250364.149079151689313No Hit
ATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGTCTT196163.2508522383582665Illumina PCR Primer Index 9 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGTC57830.9583849150910407No Hit
CTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGTC31080.5150718167219358RNA PCR Primer, Index 47 (95% over 22bp)
TCTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCG28680.4752979312607824No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCC14940.24759243699567957No Hit
TATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCGTCT14630.24245497679028058No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCC11060.18329132216681499No Hit
CTTAATACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCG8240.13655700674995983No Hit
CTTATAACACATCTCCGAGCCCACGAGACCCTCGCAGATCTCGTATGCCG6430.10656086813134No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCGTA254.4043434E-544.0606651
TCGACTT207.8579504E-443.9949417
CCGTCTT21000.042.1094544
GCCGTCT22950.038.43567343
CACCGTT250.00235223135.19887524
TTTACAC8800.035.001782
CTTTACA9350.033.457831
CTATACA5250.031.0522771
TTACACA10050.030.8672433
GGACCGT300.005743661429.3323946
AGACTCG300.005745988329.32996431
GTTGTGC300.005745988329.3299648
TTATACA38600.028.669852
CTTATAC39500.028.0538161
TATACAC43700.025.726713
TGCCGTC35300.025.05094542
GCACTAG450.001386229624.4781481
ACGGGCA450.001397663724.4436635
TCGGCTA551.5935703E-423.99724242
CACCCGT651.8118879E-523.6915524
Produced by FastQC (version 0.11.5)