FastQCFastQC Report
Wed 25 May 2016
SRR1780456_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780456_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82214
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCTAT150.002218689869.99087513
AGACTAA200.00693278552.49315664
GCCTTGT200.00693278552.49315611
CATGAGG200.00693278552.4931564
GAACTAA200.00693278552.49315611
GGCGGCA200.00693278552.49315620
GTAGATC404.5610053E-543.74429737
AGATCTC350.001249781139.9947939
CGACAAT350.001249781139.9947917
TAGATCT350.001249781139.9947938
ATAAAGG350.001249781139.994794
GGTTAAC350.001249781139.9947962
GCGACAA400.002407972734.99543816
ATGGATT400.002407972734.99543822
GACAATG400.002407972734.99543818
TAAGTAA400.002407972734.99543826
GTTAACG400.002407972734.99543863
TGCGACA400.002407972734.99543815
ATAAGTA400.002407972734.99543825
TTAACGA400.002407972734.99543864
Produced by FastQC (version 0.11.5)