##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780456_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 82214 Sequences flagged as poor quality 0 Sequence length 76 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.444680954581944 34.0 23.0 34.0 12.0 34.0 2 29.21903812002822 34.0 27.0 34.0 12.0 34.0 3 29.465468168438466 34.0 27.0 34.0 11.0 34.0 4 29.79018172087479 34.0 31.0 34.0 11.0 34.0 5 29.80966745323181 34.0 31.0 34.0 11.0 34.0 6 32.07233561193957 37.0 31.0 38.0 11.0 38.0 7 31.352713649743354 37.0 27.0 38.0 11.0 38.0 8 30.957111927408956 36.0 27.0 38.0 11.0 38.0 9 31.41962439487192 37.0 27.0 38.0 12.0 38.0 10-11 31.661839832631912 37.0 27.5 38.0 11.0 38.0 12-13 31.953742671564452 37.0 29.5 38.0 11.0 38.0 14-15 31.57823485051208 37.0 27.0 38.0 11.0 38.0 16-17 31.395303719561145 37.0 27.0 38.0 11.0 38.0 18-19 31.446961588050698 37.0 27.5 38.0 11.0 38.0 20-21 31.50647091736201 37.0 27.0 38.0 11.0 38.0 22-23 31.385834529398885 37.0 27.0 38.0 11.0 38.0 24-25 31.750145960542 37.0 29.0 38.0 11.0 38.0 26-27 31.78956138857129 37.0 27.5 38.0 11.0 38.0 28-29 31.79252317123604 37.0 29.0 38.0 11.0 38.0 30-31 31.655004013914905 37.0 27.0 38.0 11.0 38.0 32-33 31.4977741017345 37.0 27.0 38.0 11.0 38.0 34-35 31.44978957355195 37.0 27.0 38.0 11.0 38.0 36-37 31.393394069136644 37.0 27.0 38.0 11.0 38.0 38-39 31.721841778772472 37.0 27.0 38.0 11.0 38.0 40-41 31.316266086068065 37.0 27.0 38.0 11.0 38.0 42-43 31.66831074999392 37.0 27.0 38.0 11.0 38.0 44-45 30.857591164521857 37.0 26.0 38.0 11.0 38.0 46-47 31.475356995158975 37.0 27.0 38.0 11.0 38.0 48-49 31.29723039871555 37.0 27.0 38.0 11.0 38.0 50-51 31.23705208358674 37.0 26.5 38.0 11.0 38.0 52-53 31.26892621694602 37.0 27.0 38.0 11.0 38.0 54-55 30.974158902376722 37.0 26.0 38.0 11.0 38.0 56-57 30.9975673243 37.0 26.5 38.0 11.0 38.0 58-59 30.882775439706133 36.5 26.5 38.0 11.0 38.0 60-61 30.968436032792468 37.0 26.0 38.0 11.0 38.0 62-63 30.967438635755467 37.0 26.0 38.0 11.0 38.0 64-65 30.985257985257988 37.0 26.0 38.0 11.0 38.0 66-67 31.041270343250538 37.0 26.0 38.0 11.0 38.0 68-69 30.60667891113436 36.0 25.0 38.0 11.0 38.0 70-71 30.496101637190748 36.0 25.0 38.0 11.0 38.0 72-73 30.60827231371786 36.0 25.0 38.0 11.0 38.0 74-75 30.26932760843652 36.0 24.5 38.0 11.0 38.0 76 29.616829250492618 34.0 24.0 37.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -2.6380606429985782 1101 2 0.01562007222026196 1101 3 -0.2923825747012714 1101 4 -2.1343928985955465 1101 5 -0.5242862094580083 1101 6 -2.500381256984877 1101 7 -1.1704987878970883 1101 8 0.22988120798984824 1101 9 -0.9735030151715485 1101 10-11 -1.4556560900945108 1101 12-13 -0.9069523229572276 1101 14-15 -0.5800524812251453 1101 16-17 -1.8430769604582586 1101 18-19 -0.01388041616628044 1101 20-21 0.02586740396142062 1101 22-23 -0.023109165708500967 1101 24-25 -0.6372938853519869 1101 26-27 -0.4510259539847077 1101 28-29 -0.39409016900051697 1101 30-31 -1.395905838850414 1101 32-33 -0.34529120770330834 1101 34-35 0.12145150277611805 1101 36-37 0.1428489683302523 1101 38-39 -0.6549694669325952 1101 40-41 -0.30336412540949453 1101 42-43 -0.8080174729383742 1101 44-45 -0.6283950108044714 1101 46-47 0.10168090007627839 1101 48-49 -1.3321368051177558 1101 50-51 -0.7298090548054645 1101 52-53 -0.3463331652815427 1101 54-55 -1.5510487589747015 1101 56-57 -1.267659850971718 1101 58-59 -0.2757518018356606 1101 60-61 0.20806857986393368 1101 62-63 -0.7045837916155904 1101 64-65 -0.5616630106013147 1101 66-67 -0.18023622242972337 1101 68-69 0.33857421480702143 1101 70-71 0.7017447273842308 1101 72-73 -0.28155490208290246 1101 74-75 -1.2389294927903514 1101 76 -2.057920755224419 1102 1 0.9107431847526186 1102 2 0.3117923210240896 1102 3 0.980344698026002 1102 4 1.4938367664762247 1102 5 1.0838477618338587 1102 6 0.8603842932543557 1102 7 2.3524677192799466 1102 8 2.266723313253003 1102 9 1.531281673823667 1102 10-11 0.2644396036853962 1102 12-13 -2.7538422751103404 1102 14-15 -0.5467988927083951 1102 16-17 -1.6055171518458167 1102 18-19 -0.14378472238637485 1102 20-21 0.7273028106599853 1102 22-23 0.3252161931431736 1102 24-25 1.0921319519685824 1102 26-27 -4.760595331975139 1102 28-29 0.7463404530090507 1102 30-31 1.1717975104318796 1102 32-33 -6.009166805789434 1102 34-35 0.6800639429675073 1102 36-37 1.3741886812489064 1102 38-39 1.3208678536415661 1102 40-41 0.7021382669350018 1102 42-43 1.206815062946788 1102 44-45 -5.560930895971936 1102 46-47 0.2016809000762798 1102 48-49 1.4527914245473212 1102 50-51 -5.121675083513598 1102 52-53 -0.8248020648030767 1102 54-55 1.2357933462884603 1102 56-57 0.08066550787995652 1102 58-59 1.1993678153892233 1102 60-61 0.9693125990026878 1102 62-63 -4.009129246161045 1102 64-65 -0.119796981893181 1102 66-67 0.3544527727855886 1102 68-69 -5.803770282800638 1102 70-71 0.6168164977191601 1102 72-73 0.6861484471993933 1102 74-75 -5.250412746378867 1102 76 -0.9746671667076718 1103 1 -0.39482920195054305 1103 2 -0.5104429018086947 1103 3 0.12154525798087334 1103 4 0.32819041558510165 1103 5 -0.10141790332148659 1103 6 -1.154575695045093 1103 7 0.06684894825552234 1103 8 1.5367761746641762 1103 9 0.6577311838631594 1103 10-11 0.384150850838612 1103 12-13 -1.4813030469287263 1103 14-15 -0.29229104707207654 1103 16-17 -0.2765750068843644 1103 18-19 0.1402565638383173 1103 20-21 -0.34441528967222723 1103 22-23 -0.28426752010978973 1103 24-25 0.5666937037079975 1103 26-27 -0.16575819944551995 1103 28-29 0.5155053262879186 1103 30-31 -0.4483420604086774 1103 32-33 -3.6689524350073768 1103 34-35 -0.8963030364102771 1103 36-37 -0.06833236929328379 1103 38-39 0.471334830929969 1103 40-41 0.3204809676725553 1103 42-43 0.5803759998265079 1103 44-45 -3.5015506072190874 1103 46-47 -0.23971188319193715 1103 48-49 -0.16977183154845932 1103 50-51 0.7013332113402484 1103 52-53 0.24605938815444617 1103 54-55 0.6870689151232057 1103 56-57 0.8429354144844972 1103 58-59 0.8661006458775091 1103 60-61 0.8110104323116438 1103 62-63 0.6554828597289273 1103 64-65 0.6799251300560023 1103 66-67 0.17594626435492344 1103 68-69 -2.721929117760208 1103 70-71 -0.10927572768356697 1103 72-73 1.197587823727254 1103 74-75 -0.8666242755988982 1103 76 0.5855175348238468 1104 1 -0.6026346340299682 1104 2 0.7202931899922724 1104 3 0.6316202574913206 1104 4 0.7308183357683902 1104 5 0.10807159087241658 1104 6 0.18954085771892437 1104 7 1.5552269614538652 1104 8 1.6424608634064128 1104 9 -0.017283992277988602 1104 10-11 0.8489226212578593 1104 12-13 0.003951618510789956 1104 14-15 0.8635042806346647 1104 16-17 0.2607583003394751 1104 18-19 1.3144297089222228 1104 20-21 2.0109511896588543 1104 22-23 1.3922554790873427 1104 24-25 1.4135321708198276 1104 26-27 1.0981597906459228 1104 28-29 0.7660585211104056 1104 30-31 0.22445054538371778 1104 32-33 0.05422495939605554 1104 34-35 0.2709086658632067 1104 36-37 1.0374796032134235 1104 38-39 0.8350824565982009 1104 40-41 1.6181487374651837 1104 42-43 1.6784824090536006 1104 44-45 -2.213762015760807 1104 46-47 0.678787343314422 1104 48-49 1.7449578373179335 1104 50-51 1.6448192199172134 1104 52-53 0.930395652278051 1104 54-55 0.8738627350068882 1104 56-57 1.8644855349145253 1104 58-59 1.3730327155115205 1104 60-61 0.7409456155023548 1104 62-63 1.2345358685212986 1104 64-65 0.9723351012702928 1104 66-67 0.8474366593262381 1104 68-69 -3.839558898244672 1104 70-71 0.48478697425701966 1104 72-73 1.224640519205746 1104 74-75 -0.09583296222627169 1104 76 1.1845149304022016 1105 1 -1.538897463082261 1105 2 -0.32393068752428533 1105 3 -1.0342896436288207 1105 4 0.293420358396304 1105 5 0.5351500380645646 1105 6 0.17361125567752822 1105 7 1.2725093600879411 1105 8 0.5230545178334935 1105 9 -0.3413735282743886 1105 10-11 1.0191291994892886 1105 12-13 -0.14247313335141598 1105 14-15 -0.11197276310138449 1105 16-17 0.7239457217703276 1105 18-19 0.7382997203675252 1105 20-21 0.22569123131222568 1105 22-23 0.04233897346789561 1105 24-25 -0.041709212372467164 1105 26-27 -0.4109026331302701 1105 28-29 -0.6649225847679716 1105 30-31 -0.4381101991234786 1105 32-33 0.5885889948952396 1105 34-35 0.24813064833876552 1105 36-37 1.0865067529591919 1105 38-39 0.6218528189075272 1105 40-41 0.6755722322209863 1105 42-43 1.0073095475416949 1105 44-45 1.1122788495787077 1105 46-47 0.7712690965087852 1105 48-49 1.0402371213301578 1105 50-51 0.7055355829395253 1105 52-53 -0.4708652066821202 1105 54-55 -0.4953231478758262 1105 56-57 0.07790280042665287 1105 58-59 0.3595047495847332 1105 60-61 0.1612506983400408 1105 62-63 0.2243195336431647 1105 64-65 -0.6513679214139145 1105 66-67 -0.3131915186199876 1105 68-69 0.4791269564938716 1105 70-71 1.168437085895576 1105 72-73 -0.311008766870394 1105 74-75 -0.256348563479623 1105 76 -0.5733138404479377 1106 1 0.03868354304793442 1106 2 -0.6202428408764327 1106 3 -1.321513541653534 1106 4 -0.9563023233201484 1106 5 -0.5572115460553135 1106 6 -1.6178843169603994 1106 7 0.10306302360842068 1106 8 0.6251076461783427 1106 9 0.8535471684269815 1106 10-11 0.027575244054819592 1106 12-13 -0.7332681124309097 1106 14-15 -0.3803584787771612 1106 16-17 -0.01180401064186043 1106 18-19 -1.0258143206950443 1106 20-21 -1.3921252520973297 1106 22-23 -1.1679072073241663 1106 24-25 -0.6311127348011922 1106 26-27 -0.8456403970691646 1106 28-29 -1.9083496549246313 1106 30-31 -1.5131029012739141 1106 32-33 -0.605572725451168 1106 34-35 -0.5327100639313507 1106 36-37 -0.8223285102499212 1106 38-39 -0.9498899075690694 1106 40-41 -1.0567912979921132 1106 42-43 -0.379865698152571 1106 44-45 0.3515315497830471 1106 46-47 -0.5981967009029141 1106 48-49 -0.7435811135632875 1106 50-51 -0.8121835958091381 1106 52-53 -0.8349500563464147 1106 54-55 -0.6802409254373707 1106 56-57 0.04470245012987206 1106 58-59 -0.6706110429617311 1106 60-61 -0.4205727910050996 1106 62-63 -0.851646215116201 1106 64-65 -0.18914159077267456 1106 66-67 -0.08653977200132701 1106 68-69 0.24377617319135325 1106 70-71 -0.919613901746736 1106 72-73 -0.4788009241147293 1106 74-75 -1.3554533605994052 1106 76 -1.3803197760322519 1107 1 -0.2829758972358647 1107 2 -0.07483487871639838 1107 3 0.0648877196787474 1107 4 0.6759996080949087 1107 5 0.09136945512718597 1107 6 -0.055822827011638765 1107 7 -0.30769771742429697 1107 8 0.9562862035295439 1107 9 -0.08188838537672183 1107 10-11 -0.16740898929165482 1107 12-13 -0.026488451413964498 1107 14-15 0.46392610931901856 1107 16-17 0.49476871304754155 1107 18-19 0.9733185133609261 1107 20-21 0.45909666355107603 1107 22-23 -0.1169316456371341 1107 24-25 -0.09955971942870434 1107 26-27 0.5443576320991497 1107 28-29 -0.6484166632021235 1107 30-31 0.14577569549392067 1107 32-33 1.0913724854278257 1107 34-35 0.3607467748546185 1107 36-37 0.01664468643908279 1107 38-39 0.5254944046106687 1107 40-41 0.8588571056342147 1107 42-43 0.3938335529313832 1107 44-45 0.6066852746549429 1107 46-47 0.8388352265704775 1107 48-49 0.6604590914032045 1107 50-51 0.95007497730872 1107 52-53 0.7628327580012382 1107 54-55 0.6414579314802467 1107 56-57 0.6450966164680665 1107 58-59 0.7398146566835209 1107 60-61 0.030905332763129678 1107 62-63 0.6163573323808436 1107 64-65 1.0090550982300854 1107 66-67 0.8664854546443195 1107 68-69 1.1655144467427938 1107 70-71 -0.13210897466750993 1107 72-73 0.37009541013832603 1107 74-75 0.06472795591616531 1107 76 0.33266800481126424 1108 1 -0.9751914446863417 1108 2 -0.10512165551680042 1108 3 0.11312486478351857 1108 4 -0.2880460187376741 1108 5 -0.32204326031299146 1108 6 -0.20795398627646122 1108 7 0.9787572636241215 1108 8 0.7290817409678638 1108 9 0.3892551460543032 1108 10-11 0.09009118949687078 1108 12-13 0.3452129002273203 1108 14-15 0.33503745881483127 1108 16-17 -0.34872874815535226 1108 18-19 -0.3827567208520435 1108 20-21 0.21889427493632851 1108 22-23 -0.7195115641095668 1108 24-25 -0.9802541340750608 1108 26-27 -0.7415878016418276 1108 28-29 -0.13638419520527023 1108 30-31 0.16961714582647147 1108 32-33 0.2029544063317772 1108 34-35 0.36578861137038743 1108 36-37 0.29118341203445297 1108 38-39 0.6535248701759997 1108 40-41 0.34576978531669056 1108 42-43 0.3940034020338672 1108 44-45 0.1641210895730545 1108 46-47 0.24931581013623472 1108 48-49 0.12707927305235245 1108 50-51 -0.4387364365509683 1108 52-53 -0.03442998962087529 1108 54-55 0.29097877822272267 1108 56-57 0.09789644483108262 1108 58-59 -1.3514568873342157 1108 60-61 -0.17774088049582915 1108 62-63 -0.10965818646347003 1108 64-65 -0.3873458886825958 1108 66-67 -0.26001636899867364 1108 68-69 0.03183426366333819 1108 70-71 0.9021111497693113 1108 72-73 0.36617994135480814 1108 74-75 0.4919610246424746 1108 76 0.362865387347199 1109 1 -0.08375029817099033 1109 2 -0.7657320148940734 1109 3 0.19536697157822047 1109 4 -0.47522774433057435 1109 5 -0.16761651798795185 1109 6 0.15633380653599005 1109 7 -0.7971775537773063 1109 8 0.6821044384798647 1109 9 -0.16656109139659137 1109 10-11 0.5303920042793528 1109 12-13 0.204662922051849 1109 14-15 0.32033318120679866 1109 16-17 -0.5089571782441027 1109 18-19 0.042539729024280604 1109 20-21 0.6475098722009847 1109 22-23 0.5393000901898652 1109 24-25 0.6844846990400271 1109 26-27 0.762358801006215 1109 28-29 0.2312954934314213 1109 30-31 0.511716665686798 1109 32-33 0.8466325672513193 1109 34-35 0.47807582219535405 1109 36-37 0.04999506633388151 1109 38-39 0.28250784704199816 1109 40-41 0.509317362793773 1109 42-43 0.6474498591850377 1109 44-45 0.30949065162747047 1109 46-47 0.11411284200005056 1109 48-49 0.1373731767334263 1109 50-51 0.49789058221812965 1109 52-53 0.6089753646640119 1109 54-55 0.3968641266877313 1109 56-57 0.11167317988127579 1109 58-59 -0.30533437896814775 1109 60-61 0.7153734800454217 1109 62-63 0.12826557317569964 1109 64-65 0.79046947578707 1109 66-67 0.27348428573724703 1109 68-69 1.2096041603605592 1109 70-71 -0.12538775900415544 1109 72-73 -0.36606034672900023 1109 74-75 0.8232755888793442 1109 76 -0.5967592307235101 1110 1 0.12008913673710353 1110 2 -0.666897896710875 1110 3 -0.01392210634977431 1110 4 0.33815521176846985 1110 5 -0.4381048971463919 1110 6 0.07851974000097783 1110 7 0.47640591880358585 1110 8 0.6815158010805042 1110 9 -1.4076555771933421 1110 10-11 -1.1508798322693288 1110 12-13 -0.3407570992150539 1110 14-15 -1.1025078487187656 1110 16-17 -0.2136890634028461 1110 18-19 0.08348800488635177 1110 20-21 -0.023935517438992804 1110 22-23 -0.06934148659883377 1110 24-25 0.6604455071822102 1110 26-27 -0.815018534555076 1110 28-29 -0.2262371664500904 1110 30-31 -0.5121706197453832 1110 32-33 0.6750542594889026 1110 34-35 -0.10513092244994482 1110 36-37 0.34003190364209956 1110 38-39 0.7607852281752159 1110 40-41 0.14703003178966867 1110 42-43 0.856995279206302 1110 44-45 1.668312617296614 1110 46-47 0.33859256249222724 1110 48-49 0.22961835370441008 1110 50-51 -0.11893495325202252 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2116 34-35 -1.7625197890899145 2116 36-37 -0.4105003139663985 2116 38-39 -0.9494670745880995 2116 40-41 -0.6806368526822197 2116 42-43 -0.6616059896847872 2116 44-45 0.6591647978079678 2116 46-47 -0.8078884779141511 2116 48-49 -0.796012403203882 2116 50-51 0.03382730883089735 2116 52-53 -0.4898738351380061 2116 54-55 -0.49147938098427346 2116 56-57 -0.8834493246559276 2116 58-59 -1.4944120889169987 2116 60-61 -1.9373859655906145 2116 62-63 -0.6861627389840095 2116 64-65 0.20556186978145874 2116 66-67 -1.2149252176450354 2116 68-69 -0.39467937370972805 2116 70-71 -2.899929913764094 2116 72-73 -0.500454423614002 2116 74-75 -1.4083074832209697 2116 76 -2.3622269753201124 2117 1 0.6212153751009701 2117 2 0.7562487933443407 2117 3 1.0310277610929681 2117 4 0.011665686781924478 2117 5 -1.2449101675432637 2117 6 -1.522672278656703 2117 7 -2.0966002406239284 2117 8 -2.2079135050370624 2117 9 0.7148751701106626 2117 10-11 0.49135510304671826 2117 12-13 1.2386775889265529 2117 14-15 0.30193132444287585 2117 16-17 0.24650389212568768 2117 18-19 0.6196870999089761 2117 20-21 0.6125785256570637 2117 22-23 0.44493084381757697 2117 24-25 1.1306151406065723 2117 26-27 1.211941419199384 2117 28-29 0.9476221434548258 2117 30-31 1.0201813247599674 2117 32-33 1.5114008618370605 2117 34-35 -0.11175878538773176 2117 36-37 -0.621297256961487 2117 38-39 0.4818369155394251 2117 40-41 0.03091784648857754 2117 42-43 -0.06591220978047829 2117 44-45 1.2144485233703328 2117 46-47 0.4801995950036435 2117 48-49 -0.3139062740389278 2117 50-51 0.7180594853231383 2117 52-53 1.4744574991623693 2117 54-55 -0.47413758964867014 2117 56-57 -0.6205295818600227 2117 58-59 -0.2603195560628535 2117 60-61 0.9165944193495221 2117 62-63 1.04615447940132 2117 64-65 0.16156871123721572 2117 66-67 -0.27960511978623614 2117 68-69 0.18919340985345912 2117 70-71 0.9214994308850635 2117 72-73 1.0710885844615134 2117 74-75 -0.21044652065412706 2117 76 -0.8182446418640943 2118 1 -0.27384045951233915 2118 2 -0.1335349253654421 2118 3 -0.7597896244356974 2118 4 -0.35327266682345737 2118 5 -0.203957865662737 2118 6 0.05881719786785311 2118 7 -0.1518121630781586 2118 8 -2.978327386891852 2118 9 -0.7236237298021599 2118 10-11 -1.2660134054011216 2118 12-13 -0.481433250045594 2118 14-15 -1.5620418583376505 2118 16-17 -1.0655057388261966 2118 18-19 -1.1573376831870412 2118 20-21 -3.172997878510813 2118 22-23 -0.8366292043371821 2118 24-25 -0.7828515869454229 2118 26-27 -0.2889303479972618 2118 28-29 -0.686630004828622 2118 30-31 -0.6304060802796734 2118 32-33 -0.268758131894522 2118 34-35 -0.22874164063286173 2118 36-37 -1.0644663382849089 2118 38-39 -0.7734870912686631 2118 40-41 -0.6763240481521322 2118 42-43 -0.9810498244593759 2118 44-45 -1.0012100759903717 2118 46-47 -1.3291013307300936 2118 48-49 -0.9478055641713539 2118 50-51 -0.7807989147764935 2118 52-53 -2.3765118229348516 2118 54-55 -0.17668854651694943 2118 56-57 0.1306742871258173 2118 58-59 -0.462642192951062 2118 60-61 -2.2966875765822294 2118 62-63 -0.45849494564745896 2118 64-65 -0.8072448551208709 2118 66-67 -1.841510968928997 2118 68-69 0.4856046348939351 2118 70-71 -0.9187284739677715 2118 72-73 -1.8078706915565874 2118 74-75 0.1423597394645988 2118 76 -0.6848642607772923 2119 1 1.0954368939472339 2119 2 1.9787261131847451 2119 3 0.6547265774895514 2119 4 1.103589989659575 2119 5 1.3588613145243649 2119 6 1.5245604075212142 2119 7 -0.6149115439250892 2119 8 1.3009468030118825 2119 9 0.6234635391452805 2119 10-11 0.6951087530478297 2119 12-13 1.2834806560419914 2119 14-15 0.3606656935220727 2119 16-17 -0.1628980649436116 2119 18-19 -0.07907357146832794 2119 20-21 -0.41960835439999755 2119 22-23 1.3598050162982425 2119 24-25 1.1487704133257424 2119 26-27 1.3861272739126207 2119 28-29 1.2648546067319124 2119 30-31 1.2863472075587126 2119 32-33 1.4180611386798887 2119 34-35 1.5064009453480622 2119 36-37 0.010894081112205356 2119 38-39 0.866251689406532 2119 40-41 1.0034676090991894 2119 42-43 0.148373504505237 2119 44-45 0.2977911442383103 2119 46-47 1.0045069306700007 2119 48-49 -0.12986820257692244 2119 50-51 0.8904398433018734 2119 52-53 0.08988669471326816 2119 54-55 -0.18318513439601247 2119 56-57 1.8157987956448274 2119 58-59 2.556734586549098 2119 60-61 1.4068759430996067 2119 62-63 0.9176744401707708 2119 64-65 1.2886221851466786 2119 66-67 1.1121542364761687 2119 68-69 0.5473504657792745 2119 70-71 0.453429974985994 2119 72-73 -1.3657524649556017 2119 74-75 0.6982755888793442 2119 76 2.055950128882401 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 3.0 12 24.0 13 175.0 14 646.0 15 1318.0 16 2010.0 17 2436.0 18 2647.0 19 2527.0 20 2072.0 21 1835.0 22 1382.0 23 1065.0 24 1085.0 25 1008.0 26 1067.0 27 1230.0 28 1401.0 29 1690.0 30 2044.0 31 2622.0 32 3166.0 33 4126.0 34 5715.0 35 8516.0 36 14083.0 37 16321.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.067512926703756 27.20366603768961 9.36849070012498 28.36033033548166 2 18.212226628068212 23.33909066582334 29.425645267229427 29.023037438879022 3 16.090933417666093 25.107645899725107 27.371250638577372 31.43017004403143 4 11.872673753861873 20.281217310920283 32.34242343153234 35.5036855036855 5 11.207336949911207 37.167635682487166 31.245286690831247 20.379740676770382 6 24.44109275792444 36.32106453888632 18.071131437468072 21.166711265721165 7 22.020580436422023 30.735641131680737 19.6998078186197 27.543970613277548 8 19.685211764419684 37.097088087187096 18.625781497068626 24.591918651324594 9 20.93925608777094 22.317366871822315 19.467487289269467 37.27588975113728 10-11 20.841340890845842 26.916340282676916 25.863599873500863 26.378718952976378 12-13 20.928917216045928 24.594959495949595 25.447004159875448 29.02911912812903 14-15 20.452112778845454 26.18836207945119 22.967499452647967 30.39202568905539 16-17 20.964191013695967 28.51035103510351 24.174106599849175 26.351351351351347 18-19 19.751502177244753 27.48619456540249 24.914248181574912 27.84805507577785 20-21 19.24246478701924 27.896708589777898 25.246308414625247 27.614518208577614 22-23 21.377137713771376 27.451528936677448 24.868027343274868 26.303306006276305 24-25 20.46062714379546 29.215826988104215 24.529277252049532 25.794268616050793 26-27 18.750456126693752 30.010095604155012 25.735276230325738 25.504172038825505 28-29 20.58773444912059 27.72216410830272 27.47889653830248 24.21120490427421 30-31 20.20641253314521 27.740409176052736 27.16264869730216 24.890529593499892 32-33 18.79241978251879 29.785681270829784 26.341620648551338 25.08027829810008 34-35 19.483299681319483 28.895321964628895 26.308171357676308 25.313206996375314 36-37 20.186951127545186 27.939888583452937 26.361082054151364 25.51207823485051 38-39 20.076264383195074 27.315907266402316 27.514778504877512 25.093049845525094 40-41 19.344028997494345 26.823290437151826 27.852312258252855 25.98036830710098 42-43 18.286423236918285 26.839710998126844 28.14909869365315 26.724767071301724 44-45 18.1988469117182 27.085411243827085 28.496971318753495 26.218770525701217 46-47 18.56070742209356 27.302527550052304 27.99036660422799 26.146398423626145 48-49 18.131948329968132 28.102269686428098 27.283674313377283 26.482107670226483 50-51 18.17026297224317 27.525117376602527 27.122509548252122 27.18211010290218 52-53 17.181988469117183 27.536064417252536 27.61208553287761 27.66986158075267 54-55 17.305446760892305 27.723380446152724 26.97776534410198 27.99340744885299 56-57 18.02126158561802 27.695404675602695 26.784367625951784 27.4989661128275 58-59 17.93976694966794 27.354830077602355 26.533193859926534 28.17220911280317 60-61 17.30787943659231 26.62989271900163 27.16143235945216 28.9007954849539 62-63 17.62169460189262 27.024594351327025 26.643272435351644 28.710438611428714 64-65 18.464616731943465 27.380373172452384 25.45977570730046 28.695234388303696 66-67 17.796847252292796 28.93606928260394 24.577322597124578 28.68976086797869 68-69 17.54263264164254 28.486024278103482 25.286447563675285 28.684895516578685 70-71 18.001800180018 29.027294621354027 25.082710973800083 27.88819422482789 72-73 18.24993310141825 28.810786484053814 24.954995499549955 27.984284914977987 74-75 17.936726105042936 29.156226433454158 24.664898922324667 28.242148539178242 76 17.781643029167782 28.053616172428054 24.77923468022478 29.385506118179382 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1.0 1 0.5 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 0.5 12 0.0 13 2.5 14 5.0 15 4.5 16 6.0 17 8.0 18 9.5 19 15.5 20 28.0 21 36.0 22 43.5 23 63.5 24 88.0 25 100.0 26 149.5 27 246.0 28 354.5 29 416.0 30 504.5 31 697.0 32 934.5 33 1068.0 34 1231.0 35 1647.5 36 2065.5 37 2230.0 38 2619.0 39 3428.5 40 4188.5 41 4811.0 42 5094.0 43 5301.0 44 5721.0 45 6040.0 46 6146.0 47 6155.5 48 5944.0 49 5477.0 50 5231.0 51 4878.0 52 4084.0 53 3400.5 54 3158.0 55 2969.0 56 2383.5 57 1748.5 58 1510.0 59 1292.0 60 955.5 61 734.5 62 632.0 63 545.5 64 382.5 65 258.5 66 183.5 67 156.0 68 134.0 69 100.0 70 83.0 71 78.0 72 67.0 73 48.0 74 36.0 75 32.0 76 26.5 77 18.5 78 12.5 79 9.0 80 11.5 81 11.5 82 5.5 83 2.0 84 4.0 85 6.0 86 6.0 87 6.0 88 6.0 89 4.5 90 4.0 91 5.0 92 5.0 93 3.5 94 3.5 95 3.5 96 2.0 97 2.0 98 5.0 99 6.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7298027100007298 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 82214.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.90587977716692 #Duplication Level Percentage of deduplicated Percentage of total 1 81.22023052030688 54.34110978665434 2 8.531796531287496 11.416547060111416 3 4.092280842089954 8.213929501058214 4 2.108860851543468 5.643807624005643 5 1.2980402137948588 4.342326124504342 6 0.8417263571246774 3.378986547303379 7 0.5526669817838054 2.5883669448025883 8 0.3599607315565575 1.9266791544019266 9 0.27087954041377305 1.631109056851631 >10 0.7217394466058248 6.440508915756441 >50 0.0018179834927098864 0.07662928455007663 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.014596054200014596 0.0 2 0.0 0.0 0.0 0.015812392050015812 0.0 3 0.0 0.0 0.0 0.01702872990001703 0.0 4 0.0 0.0 0.0 0.01702872990001703 0.0 5 0.0 0.0 0.0 0.018245067750018246 0.0 6 0.0 0.0 0.0 0.018245067750018246 0.0 7 0.0 0.0 0.0 0.018245067750018246 0.0 8 0.0 0.0 0.0 0.019461405600019462 0.0 9 0.0 0.0 0.0 0.024326757000024325 0.0 10 0.0 0.0 0.0 0.025543094850025542 0.0 11 0.0 0.0 0.0 0.025543094850025542 0.0 12 0.0 0.0 0.0 0.025543094850025542 0.0 13 0.0 0.0 0.0 0.025543094850025542 0.0 14 0.0 0.0 0.0 0.027975770550027975 0.0 15 0.0 0.0 0.0 0.02919210840002919 0.0 16 0.0 0.0 0.0 0.03405745980003406 0.0 17 0.0 0.0 0.0 0.03649013550003649 0.0 18 0.0 0.0 0.0 0.04743717615004744 0.0 19 0.0 0.0 0.0 0.04986985185004987 0.0 20 0.0 0.0 0.0 0.05351886540005352 0.0 21 0.0 0.0 0.0 0.05473520325005474 0.0 22 0.0 0.0 0.0 0.05838421680005838 0.0 23 0.0 0.0 0.0 0.06081689250006082 0.0 24 0.0 0.0 0.0 0.06324956820006325 0.0 25 0.0 0.0 0.0 0.06933125745006934 0.0 26 0.0 0.0 0.0 0.07298027100007298 0.0 27 0.0 0.0 0.0 0.08635998735008636 0.0 28 0.0 0.0 0.0 0.1192011093001192 0.0 29 0.0 0.0 0.0 0.14109519060014108 0.0 30 0.0 0.0 0.0 0.17515265040017516 0.0 31 0.0 0.0 0.0 0.20921011020020922 0.0 32 0.0 0.0 0.0 0.2761086919502761 0.0 33 0.0 0.0 0.0 0.3198968545503199 0.0 34 0.0 0.0 0.0 0.388011774150388 0.0 35 0.0 0.0 0.0 0.4768044372004768 0.0 36 0.0 0.0 0.0 0.5862748437005862 0.0 37 0.0 0.0 0.0 0.7906196025007907 0.0 38 0.0 0.0 0.0 1.0484832267010484 0.0 39 0.0 0.0 0.0 1.4036538789014037 0.0 40 0.0 0.0 0.0 1.8427518427518428 0.0 41 0.0 0.0 0.0 2.321988955652322 0.0 42 0.0 0.0 0.0 2.9265088671029265 0.0 43 0.0 0.0 0.0 3.545624832753546 0.0 44 0.0 0.0 0.0 4.024861945654025 0.0 45 0.0 0.0 0.0 4.628165519254628 0.0 46 0.0 0.0 0.0 5.147541781205147 0.0 47 0.0 0.0 0.0 5.75935971975576 0.0 48 0.0 0.0 0.0 6.262923589656263 0.0 49 0.0 0.0 0.0 6.793246892256794 0.0 50 0.0 0.0 0.0 7.302892451407303 0.0 51 0.0 0.0 0.0 7.802807307757803 0.0 52 0.0 0.0 0.0 8.468144111708469 0.0 53 0.0 0.0 0.0 9.148076969859147 0.0 54 0.0 0.0 0.0 9.76111124625976 0.0 55 0.0 0.0 0.0 10.346169752110345 0.0 56 0.0 0.0 0.0 10.8996034738609 0.0 57 0.0 0.0 0.0 11.414114384411414 0.0 58 0.0 0.0 0.0 11.938355997761938 0.0 59 0.0 0.0 0.0 12.493006057362493 0.0 60 0.0 0.0 0.0 12.945483737562945 0.0 61 0.0 0.0 0.0 13.408908458413409 0.0 62 0.0 0.0 0.0 13.970856545113971 0.0 63 0.0 0.0 0.0 14.56078040236456 0.0 64 0.0 0.0 0.0 15.066776947965067 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCCTAT 15 0.0022186898 69.990875 13 AGACTAA 20 0.006932785 52.493156 64 GCCTTGT 20 0.006932785 52.493156 11 CATGAGG 20 0.006932785 52.493156 4 GAACTAA 20 0.006932785 52.493156 11 GGCGGCA 20 0.006932785 52.493156 20 GTAGATC 40 4.5610053E-5 43.744297 37 AGATCTC 35 0.0012497811 39.99479 39 CGACAAT 35 0.0012497811 39.99479 17 TAGATCT 35 0.0012497811 39.99479 38 ATAAAGG 35 0.0012497811 39.99479 4 GGTTAAC 35 0.0012497811 39.99479 62 GCGACAA 40 0.0024079727 34.995438 16 ATGGATT 40 0.0024079727 34.995438 22 GACAATG 40 0.0024079727 34.995438 18 TAAGTAA 40 0.0024079727 34.995438 26 GTTAACG 40 0.0024079727 34.995438 63 TGCGACA 40 0.0024079727 34.995438 15 ATAAGTA 40 0.0024079727 34.995438 25 TTAACGA 40 0.0024079727 34.995438 64 >>END_MODULE