FastQCFastQC Report
Wed 25 May 2016
SRR1780456_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780456_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82214
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTCTT35434.309485002554309No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGT35384.303403313304304No Hit
CTTTACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTC12361.5033935826015035No Hit
CTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTC5660.6884472231006885No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCG3950.48045345075048046No Hit
TATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTCT2900.3527379765003527No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCC1830.22258982655022258No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCC1640.19947940740019948No Hit
CTTAATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCG1470.1788016639501788No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATATCGTATGCCGT1400.17028729900017028No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCC1200.14596054200014597No Hit
CTTAACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTC1160.14109519060014108No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCTGGGTAACTCGTATGCCGT1110.135013501350135No Hit
ATACACATCTCCGAGCCCACGAGACTGCTGGGTAACTCGTATGCCGTCTT990.12041744715012041No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCTGGGTACCTCGTATGCCGT920.11190308220011191No Hit
CTTATAACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCG910.11068674435011068No Hit
ATACACATCTCCGAGCCCACGAGACTGCTGGGTACCTCGTATGCCGTCTT830.10095604155010095No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTTC150.002217542670.0000137
AAGCGTT150.002217542670.0000136
GAAAGCG150.002217542670.0000134
CGAGACC150.002217542670.0000123
CGTCCTC150.002217542670.0000148
GCCGTCC150.002217542670.0000146
AAAGCGT150.002217542670.0000135
GATGTGC207.8849735E-570.08
TGCAGGA207.8849735E-570.012
GTGCAGG308.311366E-658.33333611
TACCAGC252.3779547E-456.00000417
TGTGCAG252.3779547E-456.00000410
GCCGATT252.3779547E-456.00000441
GGCCTCT252.3779547E-456.0000047
TTTACAC2000.054.2500042
CATGGTT200.006929210452.514
TAATACA200.006929210452.54
CACGGTT200.006929210452.559
GTCCTCT200.006929210452.549
TAAAAGC200.006929210452.514
Produced by FastQC (version 0.11.5)