##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780456_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 82214 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.9995256282385 34.0 34.0 34.0 32.0 34.0 2 33.362030797674365 34.0 34.0 34.0 33.0 34.0 3 33.52855961271803 34.0 34.0 34.0 34.0 34.0 4 33.535407594813535 34.0 34.0 34.0 34.0 34.0 5 33.546196511543044 34.0 34.0 34.0 34.0 34.0 6 37.304656141289804 38.0 38.0 38.0 36.0 38.0 7 36.91998929622692 38.0 37.0 38.0 35.0 38.0 8 36.94814751745445 38.0 37.0 38.0 35.0 38.0 9 36.716812221762716 38.0 37.0 38.0 34.0 38.0 10-11 36.46716495973922 38.0 37.0 38.0 34.0 38.0 12-13 36.307028000097304 38.0 37.0 38.0 34.0 38.0 14-15 36.50690271729876 38.0 37.0 38.0 34.0 38.0 16-17 36.5982922616586 38.0 37.0 38.0 34.0 38.0 18-19 36.644628652054394 38.0 37.0 38.0 34.0 38.0 20-21 36.38638796312064 38.0 37.0 38.0 34.0 38.0 22-23 36.42091371299292 38.0 37.0 38.0 34.0 38.0 24-25 35.69851241880945 38.0 36.5 38.0 31.5 38.0 26-27 35.88706911231664 38.0 37.0 38.0 32.0 38.0 28-29 35.81411924976281 38.0 36.5 38.0 31.5 38.0 30-31 35.501119030822 38.0 36.0 38.0 31.0 38.0 32-33 35.9510241564697 38.0 37.0 38.0 33.0 38.0 34-35 35.79317391198579 38.0 36.5 38.0 32.0 38.0 36-37 35.85430097063761 38.0 37.0 38.0 32.5 38.0 38-39 35.36337485099861 38.0 36.5 38.0 29.0 38.0 40-41 35.22656725131972 37.5 35.5 38.0 29.0 38.0 42-43 35.79547887221155 38.0 36.5 38.0 32.5 38.0 44-45 36.00674459337826 38.0 37.0 38.0 33.0 38.0 46-47 36.22487654170823 38.0 37.0 38.0 34.0 38.0 48-49 36.000048653514 38.0 37.0 38.0 32.5 38.0 50-51 36.0942053664826 38.0 37.0 38.0 33.5 38.0 52-53 35.98705816527598 38.0 37.0 38.0 33.0 38.0 54-55 36.171686087527675 38.0 37.0 38.0 34.0 38.0 56-57 36.18805191329944 38.0 37.0 38.0 34.0 38.0 58-59 36.20110321842995 38.0 37.0 38.0 34.0 38.0 60-61 35.95300070547595 38.0 37.0 38.0 32.5 38.0 62-63 36.29275427542754 38.0 37.0 38.0 34.0 38.0 64-65 36.278973167587026 38.0 37.0 38.0 34.0 38.0 66-67 35.430753886199426 38.0 36.0 38.0 31.0 38.0 68-69 34.16402924076191 37.5 34.0 38.0 23.5 38.0 70-71 33.481645461843485 37.0 34.0 38.0 22.0 38.0 72-73 32.67741503880118 37.0 32.0 38.0 15.0 38.0 74-75 33.01711995523877 37.5 33.5 38.0 11.0 38.0 76 32.65318558882915 37.0 32.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.10311144583773313 1101 2 0.34024822589187664 1101 3 0.08174081687067059 1101 4 -0.013829744995234705 1101 5 0.12730714067077287 1101 6 -1.1908626529416821 1101 7 0.0737539870323971 1101 8 0.31519566134245736 1101 9 0.590305753468769 1101 10-11 -0.27460533154019373 1101 12-13 0.49083673569170116 1101 14-15 0.5675398576538342 1101 16-17 -0.3964598991550048 1101 18-19 -0.5870581195603108 1101 20-21 -0.33402591192449904 1101 22-23 0.5309739024704712 1101 24-25 -1.6679206032438145 1101 26-27 -0.7135951479482969 1101 28-29 0.6416880837639809 1101 30-31 0.8077765402496055 1101 32-33 0.14081888943881893 1101 34-35 0.30906862015925896 1101 36-37 0.31205448272018543 1101 38-39 0.026661331771052232 1101 40-41 1.0267004822149914 1101 42-43 0.3068171822329191 1101 44-45 0.04663160245454634 1101 46-47 0.5720202826128755 1101 48-49 -1.5001854815746825 1101 50-51 -2.0195114069514304 1101 52-53 -2.0496428066393904 1101 54-55 -1.155682087242873 1101 56-57 -1.2271748462041927 1101 58-59 -1.8589253986106158 1101 60-61 -1.5027732807132281 1101 62-63 -0.8388708867070491 1101 64-65 -1.799784133484927 1101 66-67 -2.1413656544076005 1101 68-69 -1.337055134060229 1101 70-71 -1.2466575832768427 1101 72-73 -1.3545260755249053 1101 74-75 -0.5068633340804354 1101 76 -0.5121975863339472 1102 1 -0.9992836946415622 1102 2 0.11297549861915002 1102 3 0.2142767020381342 1102 4 0.2799501593109852 1102 5 0.16032149473775803 1102 6 0.21344356715400892 1102 7 0.20006977650608349 1102 8 0.5840951828735541 1102 9 0.244851208014218 1102 10-11 0.5849640464502386 1102 12-13 0.6654778840170579 1102 14-15 -0.12265152990597272 1102 16-17 0.1760281391225078 1102 18-19 -0.3832303683641385 1102 20-21 0.5209980115204758 1102 22-23 0.4958064383556362 1102 24-25 -5.106198115205537 1102 26-27 -1.0298630905320323 1102 28-29 0.2311617679745055 1102 30-31 1.6130396981443411 1102 32-33 0.24225429613738925 1102 34-35 0.8916045053267254 1102 36-37 -2.751821115365935 1102 38-39 -0.18051570172176667 1102 40-41 0.7226334965690597 1102 42-43 0.7348076128549295 1102 44-45 1.0695981096315776 1102 46-47 1.1607762634741192 1102 48-49 -3.186788352388078 1102 50-51 -0.5388893973820572 1102 52-53 -0.011126060227901746 1102 54-55 0.5165667165848831 1102 56-57 1.0108634313077687 1102 58-59 0.1542324961262267 1102 60-61 -5.941290027124708 1102 62-63 -2.1984402646974814 1102 64-65 -0.1478702578868436 1102 66-67 1.1516965465493385 1102 68-69 -0.41480633023248004 1102 70-71 -0.48708820528641184 1102 72-73 -4.932277271697153 1102 74-75 -1.459734147477569 1102 76 0.33852011701533513 1103 1 0.7289501491978925 1103 2 0.48998391883603176 1103 3 0.15949305773484213 1103 4 0.397937664795613 1103 5 0.04505018824604434 1103 6 0.11010264239271805 1103 7 0.7020233503971411 1103 8 0.5911781940935441 1103 9 0.3995909752568636 1103 10-11 0.33521999597072494 1103 12-13 -0.344488476903102 1103 14-15 -0.21865871738638276 1103 16-17 0.008010337572180504 1103 18-19 0.5228936156729702 1103 20-21 -0.41336399512375976 1103 22-23 0.5654142610087334 1103 24-25 -2.6797339794791597 1103 26-27 -0.38318834491450815 1103 28-29 -0.609334669626044 1103 30-31 0.916303315703523 1103 32-33 0.8783045249249142 1103 34-35 0.16030971416063977 1103 36-37 -0.9645830718649648 1103 38-39 -0.18931200769114298 1103 40-41 0.12324150726670524 1103 42-43 0.039763631325079984 1103 44-45 1.03162355832675 1103 46-47 1.040981440967279 1103 48-49 -1.9937501793453123 1103 50-51 -0.21209931777653424 1103 52-53 0.492404998462888 1103 54-55 -0.21900086453545242 1103 56-57 0.5767208099668011 1103 58-59 -0.11352204306016489 1103 60-61 -3.7024630072128772 1103 62-63 -1.0601648421196046 1103 64-65 0.1369545472781084 1103 66-67 -0.4806554728401409 1103 68-69 0.009014734935398394 1103 70-71 0.6192927729631066 1103 72-73 -3.770809688487443 1103 74-75 -1.8754797441011206 1103 76 -3.313116915223862 1104 1 -0.15383129478745872 1104 2 0.22165016122347936 1104 3 0.3166003873214649 1104 4 0.11319409262571867 1104 5 -0.34361568208117177 1104 6 0.3131496198928687 1104 7 0.7124606190833802 1104 8 0.5725947644814653 1104 9 0.8278323052742849 1104 10-11 -0.66451564544154 1104 12-13 0.40091462098789776 1104 14-15 0.633447339362597 1104 16-17 0.42722431137131167 1104 18-19 0.85825696186523 1104 20-21 -0.45401647128253586 1104 22-23 -0.005502377916592138 1104 24-25 0.34026915365965493 1104 26-27 0.9320739575508838 1104 28-29 1.3651008758338392 1104 30-31 0.3630987021566128 1104 32-33 0.6070921960236717 1104 34-35 0.3307230926633977 1104 36-37 1.0432204020026958 1104 38-39 1.0768383438078715 1104 40-41 1.492442752689385 1104 42-43 -0.19015001153625377 1104 44-45 0.44925138059945624 1104 46-47 0.8035874291788403 1104 48-49 -0.20811562082415236 1104 50-51 0.23122024280075237 1104 52-53 0.003968238911710387 1104 54-55 -0.06823814105452897 1104 56-57 -0.22923998663374334 1104 58-59 -1.3050547354055198 1104 60-61 0.5772479607311993 1104 62-63 -0.14343779725696493 1104 64-65 0.42637824555684034 1104 66-67 -0.32752331312838834 1104 68-69 1.3142901574195918 1104 70-71 1.0178574037422763 1104 72-73 0.8501588430495559 1104 74-75 0.13109984741591063 1104 76 -0.3881947541413595 1105 1 0.6666088800816681 1105 2 0.3912281862530307 1105 3 0.17319644338465423 1105 4 -0.10905106188578628 1105 5 0.034398089800923515 1105 6 0.29504353512261616 1105 7 0.8587352686884202 1105 8 -1.014511451628259 1105 9 0.6053244718127431 1105 10-11 0.2971519906355411 1105 12-13 -0.2547038941255195 1105 14-15 0.24982349561904726 1105 16-17 0.22847954149683147 1105 18-19 0.488123558484169 1105 20-21 0.37161161284977595 1105 22-23 0.5173975976917902 1105 24-25 0.5213769083175137 1105 26-27 0.4424497760772468 1105 28-29 1.0210758838080238 1105 30-31 0.8260984957660114 1105 32-33 0.8488787881395439 1105 34-35 -0.6772229455751599 1105 36-37 0.5093348174482202 1105 38-39 -0.2580116477488801 1105 40-41 0.49610149520772495 1105 42-43 0.397997538982537 1105 44-45 0.5997476561871977 1105 46-47 0.9415314035113553 1105 48-49 -0.08507867690610738 1105 50-51 0.6996143362769303 1105 52-53 0.5065012145013341 1105 54-55 0.5514308834684201 1105 56-57 0.4172316721838243 1105 58-59 0.256853064283014 1105 60-61 0.9092835349661428 1105 62-63 -0.5331783079798953 1105 64-65 0.45814363572889505 1105 66-67 0.6215169705648762 1105 68-69 0.3083423554795175 1105 70-71 0.4053463806077673 1105 72-73 0.8437232087737101 1105 74-75 -0.01751076856623257 1105 76 0.5902468091736495 1106 1 -1.1051922291914664 1106 2 0.104140514753567 1106 3 0.20328304453285284 1106 4 -0.22141848428532285 1106 5 -0.40255822040633404 1106 6 0.17670160654424194 1106 7 -1.0990734303482625 1106 8 -0.010385734006383984 1106 9 -0.6740761314761556 1106 10-11 0.14748769171562515 1106 12-13 1.0362467724114097 1106 14-15 -0.5626315009752929 1106 16-17 0.11792198578991275 1106 18-19 -0.015087495325303735 1106 20-21 0.1394135005602024 1106 22-23 0.5131648449429278 1106 24-25 -0.6034163192780895 1106 26-27 0.5286325142304094 1106 28-29 0.6365473248900528 1106 30-31 0.29529184012719867 1106 32-33 0.13433174133600545 1106 34-35 0.5316512394983093 1106 36-37 0.6992637850457655 1106 38-39 -0.6661171260012893 1106 40-41 0.6415719632431447 1106 42-43 0.6957400708498156 1106 44-45 0.6423476367262637 1106 46-47 0.8013348480963529 1106 48-49 1.0107080312772112 1106 50-51 -0.18493368357322026 1106 52-53 0.2862813059677123 1106 54-55 0.05129465694317048 1106 56-57 0.13004669602346297 1106 58-59 0.18030348755829095 1106 60-61 0.9147909787727002 1106 62-63 0.7545807656797336 1106 64-65 -1.4098820527015832 1106 66-67 0.21475429663279755 1106 68-69 1.4175960287304648 1106 70-71 -0.5835608880504068 1106 72-73 -0.18579902534131065 1106 74-75 -0.9037421590498411 1106 76 -0.6948169253792393 1107 1 0.649163308845587 1107 2 -0.2177357170162395 1107 3 0.03785143239252875 1107 4 0.294466419143923 1107 5 0.13877012015338153 1107 6 0.08188489389149822 1107 7 0.7491331128129488 1107 8 0.017738034223810928 1107 9 -0.4927004909558548 1107 10-11 0.7554794142225205 1107 12-13 0.28399017012524297 1107 14-15 0.4962865124263658 1107 16-17 0.6878175317102944 1107 18-19 0.6546011132675247 1107 20-21 0.654756425274428 1107 22-23 0.44196855010651603 1107 24-25 0.5958840354716202 1107 26-27 0.13896952644956428 1107 28-29 1.05408772693972 1107 30-31 0.7976962547901891 1107 32-33 1.1419785682969845 1107 34-35 0.3915842312208184 1107 36-37 1.3463765166184913 1107 38-39 0.10096998475945185 1107 40-41 0.2769681004130291 1107 42-43 0.9443506345076784 1107 44-45 1.024620005665959 1107 46-47 0.7963289356012737 1107 48-49 0.9650241526804493 1107 50-51 1.0943824378300135 1107 52-53 0.2907452397477712 1107 54-55 0.38457215004526546 1107 56-57 1.1064558406825142 1107 58-59 0.7197989546454053 1107 60-61 1.0534488423911483 1107 62-63 0.8293048492429946 1107 64-65 0.9633737612519084 1107 66-67 0.017198127929596296 1107 68-69 1.0358262218719787 1107 70-71 0.3907197583823887 1107 72-73 0.8588994374099741 1107 74-75 -0.7217161440715216 1107 76 0.08320830126640999 1108 1 -0.40404415736337995 1108 2 0.2330974375285635 1108 3 0.07772127434346743 1108 4 0.34166659078091044 1108 5 0.1740093392050852 1108 6 -0.4301475741053551 1108 7 -0.33779386995183813 1108 8 -1.1801629884465754 1108 9 -0.8943489211925737 1108 10-11 -0.3317783273207766 1108 12-13 0.9207723341815992 1108 14-15 0.525956468801958 1108 16-17 -0.5073748447468276 1108 18-19 0.263197265738377 1108 20-21 0.7642197021105872 1108 22-23 -0.44336542686552605 1108 24-25 0.7656348042622909 1108 26-27 -0.905289573886563 1108 28-29 -1.0716363666024407 1108 30-31 0.3241211993657416 1108 32-33 0.8149028334761255 1108 34-35 0.338215855335811 1108 36-37 0.9764004742813697 1108 38-39 1.0464037257306344 1108 40-41 0.764131084036002 1108 42-43 -0.8708088448601288 1108 44-45 0.7882705098496174 1108 46-47 0.6890200605387022 1108 48-49 0.8384776319052207 1108 50-51 0.9736376048184994 1108 52-53 -0.2443241302014556 1108 54-55 -0.34344580032304606 1108 56-57 -0.06755903452310008 1108 58-59 0.3278944992352635 1108 60-61 0.8995029795577025 1108 62-63 0.8396656444943744 1108 64-65 0.35683367686372236 1108 66-67 0.9376372325566464 1108 68-69 1.6829870600325947 1108 70-71 -0.7140681984222965 1108 72-73 -0.7763004481653653 1108 74-75 -1.022974815315166 1108 76 -0.10922179241877927 1109 1 -0.9610061826798386 1109 2 0.2202391514817137 1109 3 0.28010742304127234 1109 4 0.24814847642036852 1109 5 -0.06987979217858964 1109 6 -0.045672090328253034 1109 7 -0.7448031817516281 1109 8 0.09364393720452568 1109 9 0.5752422325976241 1109 10-11 0.3491015650115372 1109 12-13 0.423345810101857 1109 14-15 0.5733247941329651 1109 16-17 0.748345001026486 1109 18-19 0.5815444212745362 1109 20-21 0.8389323457887485 1109 22-23 0.2794539387681141 1109 24-25 0.12207485955109831 1109 26-27 0.05042481575406299 1109 28-29 -1.0571776149656031 1109 30-31 0.316873636438352 1109 32-33 0.5646845881684044 1109 34-35 -0.07568637076632712 1109 36-37 0.9034337930650125 1109 38-39 0.5049734914806692 1109 40-41 0.2776986673329631 1109 42-43 1.0532781622692156 1109 44-45 0.5507377730534557 1109 46-47 0.7305048561155942 1109 48-49 0.7687573496412838 1109 50-51 0.07268460030808654 1109 52-53 -0.1564713002873006 1109 54-55 -0.4207682941394282 1109 56-57 -0.11567303132216722 1109 58-59 0.06473612589029187 1109 60-61 0.5381069370726195 1109 62-63 0.17058918588823246 1109 64-65 0.9590634164243212 1109 66-67 -0.1189064892533338 1109 68-69 0.35475521076735816 1109 70-71 0.3038188232567336 1109 72-73 -0.6726975818769887 1109 74-75 -0.5014867460586601 1109 76 0.8436554082214016 1110 1 -0.5865568434727919 1110 2 -0.7535380017918669 1110 3 -0.02400924109362279 1110 4 -0.13583299099570212 1110 5 0.3362800133811845 1110 6 -0.2962417394244099 1110 7 0.5571298275146574 1110 8 0.6526405952631578 1110 9 0.6426127323697628 1110 10-11 -0.32989167515252404 1110 12-13 0.6903498356221434 1110 14-15 0.8342335743243652 1110 16-17 0.2664313598294612 1110 18-19 -0.480357447174498 1110 20-21 0.5953503922721168 1110 22-23 -0.7213910407245194 1110 24-25 -1.9463115144425203 1110 26-27 0.7839239802687246 1110 28-29 0.34778592749224657 1110 30-31 -0.07835840062681854 1110 32-33 0.5765712659748772 1110 34-35 0.9789619658583106 1110 36-37 1.2413496369052055 1110 38-39 0.4044494143122037 1110 40-41 -1.5803827545111844 1110 42-43 -0.24404068931820433 1110 44-45 0.7168379553411697 1110 46-47 0.3905572638324415 1110 48-49 0.986714587982469 1110 50-51 -0.6454910035199433 1110 52-53 0.02115478204598986 1110 54-55 -0.7900433207230506 1110 56-57 0.5411418706981905 1110 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38-39 -4.218793213683494 2116 40-41 -1.2008114795074931 2116 42-43 -1.5044268369058358 2116 44-45 -0.5476267707512008 2116 46-47 -0.9947261288703757 2116 48-49 -0.3398984002349721 2116 50-51 -1.3666405935543011 2116 52-53 -0.19533658654369646 2116 54-55 -0.26812227863043603 2116 56-57 -0.13746192754391018 2116 58-59 -2.297920613921626 2116 60-61 -1.6350699314309267 2116 62-63 0.5431865295608915 2116 64-65 -1.6861477698485672 2116 66-67 -0.7023704390965904 2116 68-69 -1.2401651819071162 2116 70-71 -0.16172935361176854 2116 72-73 -0.41553086021389873 2116 74-75 -1.1463370182909642 2116 76 -2.3696138542765333 2117 1 0.7720254915283036 2117 2 0.5346992856680117 2117 3 0.3693569159418786 2117 4 -0.19782593456417885 2117 5 0.22349670961575896 2117 6 0.17815377954224232 2117 7 0.6949495097759097 2117 8 0.22379294641032743 2117 9 -0.7241024389580062 2117 10-11 -0.426867774979101 2117 12-13 -1.0746550480663402 2117 14-15 0.10272680692747116 2117 16-17 -0.7311467293526661 2117 18-19 -0.7642193484243265 2117 20-21 0.19031321643939947 2117 22-23 0.3870467769644037 2117 24-25 -1.6460344398715847 2117 26-27 -2.0902562577363426 2117 28-29 -0.9325676914205872 2117 30-31 -0.057238939626557794 2117 32-33 -1.5129796340191461 2117 34-35 0.779478241497678 2117 36-37 -0.7067237978227965 2117 38-39 0.46967157230451306 2117 40-41 0.06264951769962579 2117 42-43 0.5977078704920302 2117 44-45 -0.1282195525823724 2117 46-47 -0.1698111058129399 2117 48-49 -0.28894470996239363 2117 50-51 -0.415808144269846 2117 52-53 0.7100737925509009 2117 54-55 0.18820931547205788 2117 56-57 0.5730752133337873 2117 58-59 -0.6117832219018808 2117 60-61 -0.26882472153246795 2117 62-63 -0.20839934586399522 2117 64-65 -0.7481647002865586 2117 66-67 -0.5491413152792362 2117 68-69 0.41973219625651126 2117 70-71 0.18945815856645254 2117 72-73 1.2373410356920544 2117 74-75 1.3166303548931282 2117 76 1.2600815277408302 2118 1 -0.01146489827616648 2118 2 0.3728411761574506 2118 3 -0.8845556582621157 2118 4 0.08641168426598966 2118 5 0.26569439320578425 2118 6 0.010006492398730416 2118 7 0.8355115920541252 2118 8 0.8216176796910801 2118 9 -1.293470200176813 2118 10-11 -0.7067154232833133 2118 12-13 -0.7367103430142379 2118 14-15 0.7964147972965279 2118 16-17 -2.1576380739497907 2118 18-19 -0.36499148508421086 2118 20-21 -0.12263045079460966 2118 22-23 0.8150637141556487 2118 24-25 1.044706147118326 2118 26-27 -2.0706207394499856 2118 28-29 0.05793627304452542 2118 30-31 -0.32032582575715907 2118 32-33 -0.14102563011694969 2118 34-35 0.03915070610808158 2118 36-37 -2.2851932237018318 2118 38-39 0.3247298976812445 2118 40-41 0.0878062282314076 2118 42-43 0.8455907215858858 2118 44-45 -2.0590178422581573 2118 46-47 -0.03954417899986851 2118 48-49 0.29001249041952093 2118 50-51 -0.8432439033299985 2118 52-53 -1.5849156217045746 2118 54-55 -1.187792178985994 2118 56-57 0.1195175729170046 2118 58-59 -0.5675202803102835 2118 60-61 -0.5945646858315285 2118 62-63 -0.8803242908596332 2118 64-65 0.17897009152714105 2118 66-67 0.6796145483929834 2118 68-69 -1.4998991707574802 2118 70-71 -0.5174204997423146 2118 72-73 0.8807467395402853 2118 74-75 0.0038319821918975094 2118 76 -0.08235692964635888 2119 1 0.5611613050048874 2119 2 -1.138750996301539 2119 3 -0.2469861730178451 2119 4 -0.2087480753037667 2119 5 -0.09574186114410566 2119 6 -0.516115440081947 2119 7 -0.556995299978233 2119 8 0.2777572376971378 2119 9 -0.7720484077964684 2119 10-11 -0.34332454336285423 2119 12-13 -0.665632022410449 2119 14-15 -0.15912594478329112 2119 16-17 -0.5235146048848449 2119 18-19 -0.25835705655797625 2119 20-21 -1.1536784916496714 2119 22-23 -0.00533670162597133 2119 24-25 0.7553876674328777 2119 26-27 0.5461144709264758 2119 28-29 0.193438148581194 2119 30-31 1.0037578728915477 2119 32-33 0.40581759309451115 2119 34-35 0.8245599346523917 2119 36-37 -0.17292088673794126 2119 38-39 -1.3731053262533166 2119 40-41 -1.0412668499084177 2119 42-43 -0.06864301999679157 2119 44-45 0.14279442329976177 2119 46-47 -0.31075130151987196 2119 48-49 -0.36147664050649553 2119 50-51 0.9803200682883855 2119 52-53 0.12955086720038622 2119 54-55 0.2162390950231341 2119 56-57 -1.0316213071842313 2119 58-59 -0.3044141204151245 2119 60-61 0.1668015183534095 2119 62-63 -0.41223101115610206 2119 64-65 -0.0526360909648389 2119 66-67 0.18343341222449538 2119 68-69 0.38338821569346493 2119 70-71 0.5510478872961428 2119 72-73 1.609630004329901 2119 74-75 0.6853974903945428 2119 76 1.1423007284184479 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 2.0 20 1.0 21 2.0 22 6.0 23 28.0 24 46.0 25 114.0 26 230.0 27 408.0 28 659.0 29 1100.0 30 1635.0 31 2531.0 32 3865.0 33 5879.0 34 8542.0 35 11025.0 36 16461.0 37 29680.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.04043107013404 22.83309412022283 11.258423139611258 31.868051670031868 2 17.63811516286764 18.05896805896806 42.36139830199236 21.941518476171943 3 15.852531199065853 27.002700270027002 34.37614031673438 22.768628214172768 4 11.342350451261343 22.452380373172453 35.7980392633858 30.407229912180405 5 10.971367407010971 38.39127155958839 36.43296762108643 14.204393412314204 6 22.569148806772567 38.203955530688205 17.306054929817304 21.92084073272192 7 22.494952197922494 35.1971683654852 19.722918237769722 22.584961198822583 8 19.895638212469898 35.26041793368526 23.16028900187316 21.683654851971685 9 19.3993723696694 19.916315955919917 19.08677354221909 41.597538132191595 10-11 20.025786362420025 25.299219111100303 30.080643199455082 24.594351327024594 12-13 22.76011384922276 20.18147760722018 27.4977497749775 29.56065876857956 14-15 22.8020775050478 23.817719609798818 21.174617461746177 32.20558542340721 16-17 23.826233974748824 27.751964385627755 21.34733743644635 27.074464203177072 18-19 21.874619894421873 26.968642810226967 22.425012771547426 28.73172452380373 20-21 20.933174398520933 25.091833507675094 22.143430559272144 31.831561534531833 22-23 22.993042547497993 29.702362128104703 22.525360644172526 24.77923468022478 24-25 23.43092417349843 31.148587831756146 22.641520908847642 22.77896708589778 26-27 24.180796458024183 28.942759140778946 26.058213929501058 20.818230471695816 28-29 20.00024326757 23.545259931398547 31.332863016031332 25.12163378500012 30-31 27.496533437127496 22.477315299097476 26.00773590872601 24.01841535504902 32-33 27.75865424380276 23.043520568273042 29.14771206850415 20.05011311942005 34-35 27.664996229352667 25.591140195100593 28.305398107378306 18.438465468168438 36-37 19.975916510569977 27.33962585447734 30.736249300605735 21.948208334346948 38-39 20.61327754397061 24.304862918724304 28.54197581920354 26.539883718101542 40-41 19.643856277519646 24.843092417349844 30.755102537280756 24.757948767849758 42-43 24.017807186124017 23.892524387573893 31.61931057970662 20.470357846595473 44-45 19.329432943294332 25.817379035200815 29.400710341304396 25.452477680200452 46-47 23.92840635414893 22.116671126572115 27.853528596102855 26.101393923176104 48-49 21.906852847446906 21.572968107621573 29.046756026954046 27.47342301797747 50-51 17.786508380567785 22.144038728197142 32.65319775220765 27.416255139027417 52-53 19.13785973191914 21.886783272921885 36.37397523536137 22.6013817597976 54-55 17.7980635901428 22.206071958547206 33.2048069671832 26.79105748412679 56-57 22.57158148247257 21.93057143552193 30.17186853820517 25.325978543800325 58-59 18.404408008368403 27.894884083002896 32.05111051645705 21.64959739217165 60-61 19.704673170019703 34.26910258593427 25.444571484175444 20.58165275987058 62-63 15.746101637190746 39.88979979078989 23.485659376748487 20.87843919527088 64-65 16.064782153891063 40.34592648454034 22.595908239472596 20.99338312209599 66-67 15.741844454715743 39.771815019339776 22.25411730362225 22.23222322232223 68-69 15.757048677840757 38.097525968813095 22.493127691147492 23.652297662198652 70-71 16.562264334541563 35.763981803585764 22.973581141897974 24.7001727199747 72-73 16.895540905441894 33.24008076483324 23.25090617169825 26.613472158026614 74-75 17.325516335417326 32.54920086603255 23.46923881577347 26.656043982776655 76 17.505534337217505 32.57960931228258 23.24908166492325 26.665774685576665 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 0.5 11 0.0 12 0.0 13 0.0 14 0.5 15 1.5 16 3.0 17 4.0 18 3.0 19 4.5 20 11.5 21 16.0 22 18.0 23 37.5 24 68.5 25 82.0 26 124.0 27 193.5 28 278.0 29 335.0 30 409.5 31 597.0 32 880.0 33 1050.0 34 1175.5 35 1500.5 36 1893.5 37 2087.0 38 2353.5 39 3115.0 40 4101.0 41 5748.5 42 6905.0 43 7657.0 44 8560.5 45 8219.5 46 7727.0 47 7139.5 48 5924.5 49 4932.5 50 4568.0 51 4119.5 52 3240.5 53 2584.0 54 2358.0 55 2240.5 56 1684.5 57 1106.5 58 967.0 59 792.0 60 539.5 61 368.5 62 275.0 63 232.5 64 141.5 65 92.0 66 62.5 67 34.0 68 27.5 69 17.5 70 8.0 71 2.0 72 2.0 73 1.0 74 0.5 75 1.0 76 0.5 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 82214.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.75130756318875 #Duplication Level Percentage of deduplicated Percentage of total 1 60.557456291785684 23.46680614007347 2 16.434916350167928 12.737489965212736 3 8.29592893687812 9.644342812659644 4 4.676857402931668 7.249373586007249 5 2.5926739696788976 5.023475320505024 6 1.8581876392856023 4.32043204320432 7 1.271226341065319 3.4483178047534486 8 0.9102608368122038 2.8219038120028217 9 0.6811262123732698 2.3755078210523752 >10 2.642895257227157 15.015690758265016 >50 0.03766596566119464 1.0655119566010653 >100 0.02824947424589598 2.0264188581020264 >500 0.00313883047176622 0.6884472231006885 >1k 0.00941649141529866 10.116281898460116 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTCTT 3543 4.309485002554309 No Hit CTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGT 3538 4.303403313304304 No Hit CTTTACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTC 1236 1.5033935826015035 No Hit CTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTC 566 0.6884472231006885 No Hit TCTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCG 395 0.48045345075048046 No Hit TATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTCT 290 0.3527379765003527 No Hit TCTTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCC 183 0.22258982655022258 No Hit CTCTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCC 164 0.19947940740019948 No Hit CTTAATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCG 147 0.1788016639501788 No Hit CTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATATCGTATGCCGT 140 0.17028729900017028 No Hit TCTTAATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCC 120 0.14596054200014597 No Hit CTTAACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTC 116 0.14109519060014108 No Hit CTTATACACATCTCCGAGCCCACGAGACTGCTGGGTAACTCGTATGCCGT 111 0.135013501350135 No Hit ATACACATCTCCGAGCCCACGAGACTGCTGGGTAACTCGTATGCCGTCTT 99 0.12041744715012041 No Hit CTTATACACATCTCCGAGCCCACGAGACTGCTGGGTACCTCGTATGCCGT 92 0.11190308220011191 No Hit CTTATAACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCG 91 0.11068674435011068 No Hit ATACACATCTCCGAGCCCACGAGACTGCTGGGTACCTCGTATGCCGTCTT 83 0.10095604155010095 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.023110419150023112 0.0 2 0.0 0.0 0.0 0.024326757000024325 0.0 3 0.0 0.0 0.0 0.025543094850025542 0.0 4 0.0 0.0 0.0 0.02675943270002676 0.0 5 0.0 0.0 0.0 0.02675943270002676 0.0 6 0.0 0.0 0.0 0.02675943270002676 0.0 7 0.0 0.0 0.0 0.02675943270002676 0.0 8 0.0 0.0 0.0 0.027975770550027975 0.0 9 0.0 0.0 0.0 0.03405745980003406 0.0 10 0.0 0.0 0.0 0.03649013550003649 0.0 11 0.0 0.0 0.0 0.037706473350037704 0.0 12 0.0 0.0 0.0 0.037706473350037704 0.0 13 0.0 0.0 0.0 0.037706473350037704 0.0 14 0.0 0.0 0.0 0.04257182475004257 0.0 15 0.0 0.0 0.0 0.04378816260004379 0.0 16 0.0 0.0 0.0 0.04865351400004865 0.0 17 0.0 0.0 0.0 0.04865351400004865 0.0 18 0.0 0.0 0.0 0.06081689250006082 0.0 19 0.0 0.0 0.0 0.06446590605006447 0.0 20 0.0 0.0 0.0 0.06811491960006812 0.0 21 0.0 0.0 0.0 0.07054759530007054 0.0 22 0.0 0.0 0.0 0.07054759530007054 0.0 23 0.0 0.0 0.0 0.07298027100007298 0.0 24 0.0 0.0 0.0 0.07662928455007663 0.0 25 0.0 0.0 0.0 0.08514364950008514 0.0 26 0.0 0.0 0.0 0.09000900090009001 0.0 27 0.0 0.0 0.0 0.10217237940010217 0.0 28 0.0 0.0 0.0 0.1337971635001338 0.0 29 0.0 0.0 0.0 0.16055659620016055 0.0 30 0.0 0.0 0.0 0.20191208310020192 0.0 31 0.0 0.0 0.0 0.2396185564502396 0.0 32 0.0 0.0 0.0 0.30894981390030896 0.0 33 0.0 0.0 0.0 0.3576033279003576 0.0 34 0.0 0.0 0.0 0.433016274600433 0.0 35 0.0 0.0 0.0 0.5303233026005303 0.0 36 0.0 0.0 0.0 0.6446590605006447 0.0 37 0.0 0.0 0.0 0.8575181842508576 0.0 38 0.0 0.0 0.0 1.1202471598511203 0.0 39 0.0 0.0 0.0 1.4851485148514851 0.0 40 0.0 0.0 0.0 1.9461405600019461 0.0 41 0.0 0.0 0.0 2.4606514705524605 0.0 42 0.0 0.0 0.0 3.087065463303087 0.0 43 0.0 0.0 0.0 3.7694309971537696 0.0 44 0.0 0.0 0.0 4.283941907704284 0.0 45 0.0 0.0 0.0 4.92008660325492 0.0 46 0.0 0.0 0.0 5.4552752572554555 0.0 47 0.0 0.0 0.0 6.112097696256112 0.0 48 0.0 0.0 0.0 6.655800715206656 0.0 49 0.0 0.0 0.0 7.225046829007225 0.0 50 0.0 0.0 0.0 7.765100834407765 0.0 51 0.0 0.0 0.0 8.296640474858297 0.0 52 0.0 0.0 0.0 8.98022234655898 0.0 53 0.0 0.0 0.0 9.722188435059723 0.0 54 0.0 0.0 0.0 10.370496509110371 0.0 55 0.0 0.0 0.0 11.002992191111003 0.0 56 0.0 0.0 0.0 11.581969007711582 0.0 57 0.0 0.0 0.0 12.153647797212153 0.0 58 0.0 0.0 0.0 12.704648843262705 0.0 59 0.0 0.0 0.0 13.304303403313304 0.0 60 0.0 0.0 0.0 13.784756854063785 0.0 61 0.0 0.0 0.0 14.283455372564283 0.0 62 0.0 0.0 0.0 14.863648527014863 0.0 63 0.0 0.0 0.0 15.487629844065488 0.0 64 0.0 0.0 0.0 16.036198214416036 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGCGTTC 15 0.0022175426 70.00001 37 AAGCGTT 15 0.0022175426 70.00001 36 GAAAGCG 15 0.0022175426 70.00001 34 CGAGACC 15 0.0022175426 70.00001 23 CGTCCTC 15 0.0022175426 70.00001 48 GCCGTCC 15 0.0022175426 70.00001 46 AAAGCGT 15 0.0022175426 70.00001 35 GATGTGC 20 7.8849735E-5 70.0 8 TGCAGGA 20 7.8849735E-5 70.0 12 GTGCAGG 30 8.311366E-6 58.333336 11 TACCAGC 25 2.3779547E-4 56.000004 17 TGTGCAG 25 2.3779547E-4 56.000004 10 GCCGATT 25 2.3779547E-4 56.000004 41 GGCCTCT 25 2.3779547E-4 56.000004 7 TTTACAC 200 0.0 54.250004 2 CATGGTT 20 0.0069292104 52.5 14 TAATACA 20 0.0069292104 52.5 4 CACGGTT 20 0.0069292104 52.5 59 GTCCTCT 20 0.0069292104 52.5 49 TAAAAGC 20 0.0069292104 52.5 14 >>END_MODULE