FastQCFastQC Report
Wed 25 May 2016
SRR1780455_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780455_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences558609
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAATAAAGGTGGATGCGACAATGGATTTTACATAATGGGGGTATGAGTT5920.10597752631984088No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAC353.2072485E-431.44260610
CGTGTAT300.005724855729.35169835
AGATCGG300.005777767429.29650142
TATAGAC300.00579042229.2833883
CGTTCGG655.889269E-727.09387224
TATACGC508.430706E-526.3550473
CGTTGAG1151.2732926E-1124.84723530
CGACAAT1001.7025741E-924.24560217
GTAGCCG1102.0190782E-1023.99134625
TAAGGTG651.779958E-523.74548720
GTAACCT651.779958E-523.74548720
CTTATAA651.809892E-523.6943841
TTCGGTA651.8237568E-523.67103426
TCTCGGG1053.108653E-923.01661743
TCGGTGG2800.022.75710142
GCGTGTA1407.2759576E-1222.01377134
GCGAAAT500.002572035622.00982311
GTCCTAT803.5958092E-622.00982311
TCGGACG500.002572035622.00982311
TACGGTC602.861438E-422.0098210
Produced by FastQC (version 0.11.5)