FastQCFastQC Report
Wed 25 May 2016
SRR1780455_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780455_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences558609
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGT291605.22010923561919No Hit
ATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTCTT250904.491513742170284Illumina PCR Primer Index 6 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTC78391.4033071432791093No Hit
CTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTC41280.7389784267707824No Hit
TCTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCG35240.6308527073498637No Hit
TATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTCT22850.4090517696635751No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCC16300.29179622956307544No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCC13910.24901138363327477No Hit
CTTAATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCG11510.20604752161171766No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCC9430.16881217452636818No Hit
CTTAACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCGTC6750.1208358619356294No Hit
CTTATAACACATCTCCGAGCCCACGAGACAACGCCATATCTCGTATGCCG6380.11421226654063933No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTT207.85127E-444.0019726
TCGCGGG207.854732E-443.9980344
CCGTCTT27050.042.29015744
GCCGTCT30100.037.93185443
CCTCGTG601.9826984E-1036.6617477
CTTTACA11000.035.6207241
GATCTAT250.00234500635.2204971
TTTACAC11350.034.503752
CTTATAA654.638423E-1033.8658641
TCGTGGA654.656613E-1033.8537339
CTATACA5600.033.41231
CTCGCTT401.8188854E-532.99852416
TTACACA12200.032.09983
CCTTGCG353.2181962E-431.4243538
TTATACA43600.029.4187472
CGTGCGA602.9325201E-729.3425310
GAGATCG300.005733612529.3425315
CTTAACT300.005743657229.33202242
CGTGGGG300.005743657229.33202222
CGTATTG300.005743657229.33202221
Produced by FastQC (version 0.11.5)