FastQCFastQC Report
Wed 25 May 2016
SRR1780454_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780454_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54457
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTAT200.00676912252.7867051
GCCTCAG200.00676912252.7867051
CGTCAGA200.00676912252.7867051
CAAACAG200.006918853652.495872
GGTCGTT200.006918853652.4958746
AGCCCAT200.006918853652.495876
TGTATAA200.006918853652.4958710
GTCAGAT200.006918853652.495872
TCAGATG200.006918853652.495873
TCGTCTT200.006918853652.4958714
ATCATGC200.006918853652.495878
GTGTATA305.832188E-446.6629949
GCCGCCG350.001245746539.99685317
AGGTAAT459.091142E-538.88582653
CCGCCGA459.091142E-538.88582618
ATCTCTG400.00240024434.99724639
GGTAATA501.6900005E-434.99724254
TCTCTGT500.00715389527.99779340
TGCCGCC500.00715389527.99779316
TAGGAGG800.00263617421.873284
Produced by FastQC (version 0.11.5)