##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780454_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 54457 Sequences flagged as poor quality 0 Sequence length 76 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.069339111592633 34.0 23.0 34.0 12.0 34.0 2 28.780395541436363 34.0 23.0 34.0 11.0 34.0 3 29.15290963512496 34.0 27.0 34.0 11.0 34.0 4 29.47648603485319 34.0 31.0 34.0 11.0 34.0 5 29.45487265181703 34.0 31.0 34.0 11.0 34.0 6 31.72431459683787 37.0 27.0 38.0 11.0 38.0 7 30.960317314578475 37.0 26.0 38.0 11.0 38.0 8 30.141818315368088 36.0 24.0 38.0 11.0 38.0 9 30.926565914391173 37.0 26.0 38.0 12.0 38.0 10-11 31.094974016196264 37.0 26.5 38.0 11.0 38.0 12-13 31.39220853150192 37.0 27.0 38.0 11.0 38.0 14-15 31.040343757460015 37.0 26.0 38.0 11.0 38.0 16-17 30.844611344730705 37.0 26.0 38.0 11.0 38.0 18-19 30.85925592669446 37.0 26.0 38.0 11.0 38.0 20-21 30.990230824320108 37.0 26.5 38.0 11.0 38.0 22-23 30.797537506656628 37.0 26.0 38.0 11.0 38.0 24-25 31.156343537102668 37.0 26.0 38.0 11.0 38.0 26-27 31.1282296123547 37.0 26.0 38.0 11.0 38.0 28-29 31.125631231981195 37.0 26.0 38.0 11.0 38.0 30-31 31.009759994123804 37.0 26.0 38.0 11.0 38.0 32-33 30.86765705051692 37.0 26.0 38.0 11.0 38.0 34-35 30.85606992673118 37.0 26.0 38.0 11.0 38.0 36-37 30.804745028187376 37.0 26.0 38.0 11.0 38.0 38-39 31.028123106304058 37.0 26.0 38.0 11.0 38.0 40-41 30.731696567934335 37.0 26.0 38.0 11.0 38.0 42-43 31.00523348697137 37.0 26.0 38.0 11.0 38.0 44-45 30.277475806599703 36.0 25.0 38.0 11.0 38.0 46-47 30.86842830122849 37.0 26.0 38.0 11.0 38.0 48-49 30.768835962318896 37.0 26.0 38.0 11.0 38.0 50-51 30.664157041335365 36.5 25.5 38.0 11.0 38.0 52-53 30.667976568668855 37.0 26.0 38.0 11.0 38.0 54-55 30.49410544099014 36.0 25.0 38.0 11.0 38.0 56-57 30.416980369833077 36.5 24.5 38.0 11.0 38.0 58-59 30.308895091540116 36.5 24.5 38.0 11.0 38.0 60-61 30.435609747139946 36.5 24.5 38.0 11.0 38.0 62-63 30.305103108874892 36.0 24.5 38.0 11.0 38.0 64-65 30.396826854215252 36.5 24.5 38.0 11.0 38.0 66-67 30.450116605762346 36.0 25.0 38.0 11.0 38.0 68-69 30.018537561745966 36.0 23.5 38.0 11.0 38.0 70-71 29.9476467671741 36.0 24.0 38.0 11.0 38.0 72-73 29.967524836109224 36.0 24.0 38.0 11.0 38.0 74-75 29.68710175000459 35.5 23.5 38.0 11.0 38.0 76 29.0656481260444 34.0 23.0 37.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -4.8712151444050384 1101 2 -2.9581800318136224 1101 3 -2.2142025678528583 1101 4 -3.99899020335517 1101 5 -3.7041132902404037 1101 6 -2.7681574425223623 1101 7 -3.299552273434994 1101 8 -3.697119277377343 1101 9 -2.8682061228438407 1101 10-11 -3.880163131605478 1101 12-13 -3.8216167056199133 1101 14-15 -3.032056785385805 1101 16-17 -3.4826447291235887 1101 18-19 -3.219850510647799 1101 20-21 -2.9856858663202566 1101 22-23 -2.8726061913580985 1101 24-25 -3.438275051240037 1101 26-27 -4.117440498611817 1101 28-29 -3.4689936570375366 1101 30-31 -3.5594838302745835 1101 32-33 -2.993526632076488 1101 34-35 -3.755289716962725 1101 36-37 -2.8106207642321586 1101 38-39 -3.7867057824117154 1101 40-41 -3.231704865487618 1101 42-43 -3.588722882589259 1101 44-45 -3.348500687552203 1101 46-47 -3.3640877718077853 1101 48-49 -3.162608760094191 1101 50-51 -3.348029758851304 1101 52-53 -1.7102779193677016 1101 54-55 -2.945970951451468 1101 56-57 -3.642623386348326 1101 58-59 -3.3295721393447053 1101 60-61 -3.6826954236520777 1101 62-63 -3.9649284547498205 1101 64-65 -4.129806540959272 1101 66-67 -4.024464598928002 1101 68-69 -1.9717108370638812 1101 70-71 -3.0963538802361015 1101 72-73 -3.229612483130932 1101 74-75 -3.588683365334912 1101 76 -3.7024058009278242 1102 1 0.5023688154947088 1102 2 0.45319841430166363 1102 3 0.10835382312458464 1102 4 0.31865390692051676 1102 5 0.8530796922157364 1102 6 0.7756520812871628 1102 7 0.47453293959758724 1102 8 1.7949107978106262 1102 9 1.8407161829205698 1102 10-11 -0.02665435967565344 1102 12-13 -2.800413698101117 1102 14-15 -1.350277336764254 1102 16-17 -1.5576572604519114 1102 18-19 -0.6961161748081999 1102 20-21 0.19702090059703892 1102 22-23 -0.30974904850095086 1102 24-25 -0.7093527454756234 1102 26-27 -4.86155077430104 1102 28-29 0.017297069779505847 1102 30-31 0.33407506696852707 1102 32-33 -4.586910090723109 1102 34-35 -0.052532824732150374 1102 36-37 0.60810104027912 1102 38-39 0.19156489428001322 1102 40-41 -0.7206522339086732 1102 42-43 0.05654027530547623 1102 44-45 -4.519904196324131 1102 46-47 -0.5521078219330988 1102 48-49 -1.0560924693673748 1102 50-51 -5.228581137297418 1102 52-53 -1.3721826812724665 1102 54-55 -0.7755448862885608 1102 56-57 -1.3770594765738906 1102 58-59 0.17970104361268824 1102 60-61 0.38394618035794537 1102 62-63 -4.303349507381398 1102 64-65 -0.0375759645181688 1102 66-67 -0.13920144103326137 1102 68-69 -6.098603067640326 1102 70-71 -0.23051428123860873 1102 72-73 0.2984326296510247 1102 74-75 -5.246578102177015 1102 76 -2.3095737207273253 1103 1 -0.2403925257281685 1103 2 0.5793917081872664 1103 3 0.3172295627695547 1103 4 0.35056712191065387 1103 5 0.5064130255490689 1103 6 -0.4968134019475521 1103 7 1.3266433932465027 1103 8 1.7717155315384367 1103 9 1.2174814689166276 1103 10-11 1.001649387858862 1103 12-13 -0.09189298804194834 1103 14-15 0.22311516816671428 1103 16-17 -0.16418586005743308 1103 18-19 0.59242425911685 1103 20-21 -0.2017956674502983 1103 22-23 0.48918586274164966 1103 24-25 0.4935663866742779 1103 26-27 0.19209814088633692 1103 28-29 0.2327014090299997 1103 30-31 0.7970139229843021 1103 32-33 -3.772945593681687 1103 34-35 -0.6357675387360935 1103 36-37 1.0597578450128502 1103 38-39 1.213872586587705 1103 40-41 1.3668625589907357 1103 42-43 1.2088479676131705 1103 44-45 -3.0627641568764012 1103 46-47 0.5472215666270586 1103 48-49 0.2772408639659609 1103 50-51 0.17303621970455296 1103 52-53 -0.11813928876753366 1103 54-55 0.2086760210092713 1103 56-57 0.8166683340770007 1103 58-59 0.2830541008118992 1103 60-61 1.1524471665512408 1103 62-63 0.7258220902517358 1103 64-65 0.23776920313468963 1103 66-67 0.35890506784247833 1103 68-69 -2.769036992689635 1103 70-71 0.8893870994320032 1103 72-73 1.337545055686526 1103 74-75 -0.9773473329462483 1103 76 0.822931210239144 1104 1 -2.3681015321535526 1104 2 0.24759514231802626 1104 3 -0.09962163699811555 1104 4 0.18339828319863472 1104 5 -0.28499801739571495 1104 6 0.021500752043809968 1104 7 0.4919971522765252 1104 8 1.016751288608173 1104 9 -0.6407971095129668 1104 10-11 -1.1279836030294383 1104 12-13 -0.7575916122115451 1104 14-15 0.6050805364463798 1104 16-17 -0.22589856924536633 1104 18-19 0.337278508218386 1104 20-21 0.6921129251369109 1104 22-23 -0.9383380055561652 1104 24-25 -0.026816958154562087 1104 26-27 0.14437969604660594 1104 28-29 -0.030412541672436788 1104 30-31 -1.496865628327999 1104 32-33 -0.0800389250789344 1104 34-35 0.297364925779096 1104 36-37 0.12288631635274072 1104 38-39 -0.3526682345543435 1104 40-41 0.13720052682752382 1104 42-43 0.3877059194772592 1104 44-45 -3.0745258732157517 1104 46-47 -0.10789514299649028 1104 48-49 0.771105894640808 1104 50-51 0.5469607849929687 1104 52-53 0.09121200175411914 1104 54-55 0.030181085081583348 1104 56-57 -0.2299429121567087 1104 58-59 0.7426867286842622 1104 60-61 0.06392573046019834 1104 62-63 0.7249327012075568 1104 64-65 -0.7938131833320767 1104 66-67 -0.19220757600258764 1104 68-69 -2.57113885496139 1104 70-71 -1.7216594755126344 1104 72-73 -0.30604589795633785 1104 74-75 -0.5778664457352995 1104 76 -0.27305020334491203 1105 1 0.08393744294568961 1105 2 -1.0577819778551962 1105 3 -0.556352059228356 1105 4 -0.9903657009226201 1105 5 -0.8229987391568123 1105 6 -1.1051322324383293 1105 7 0.23649372391131251 1105 8 -0.8203833198364308 1105 9 -0.4247740131578581 1105 10-11 -0.24920337928349667 1105 12-13 -1.887472521630528 1105 14-15 -1.2545910622544483 1105 16-17 -0.7356964761381839 1105 18-19 -0.7839593120631001 1105 20-21 0.1940797241264498 1105 22-23 -0.20033728379506854 1105 24-25 0.03456882315182597 1105 26-27 -0.4242958723402559 1105 28-29 -1.8046637145342217 1105 30-31 -0.6251406193059843 1105 32-33 -0.34985126719369575 1105 34-35 -0.18390537375175953 1105 36-37 -0.19836954795617245 1105 38-39 -0.7196115763082247 1105 40-41 -0.5518287044969092 1105 42-43 -0.5775773717533461 1105 44-45 0.8891154115190041 1105 46-47 -0.12602939056054652 1105 48-49 -0.5433473713281565 1105 50-51 -1.067600745140556 1105 52-53 -1.7241434655861916 1105 54-55 -1.1951527294258142 1105 56-57 -0.33470653539741946 1105 58-59 -0.891867583838291 1105 60-61 0.03473049408343698 1105 62-63 -0.021584801499045625 1105 64-65 -0.8542426311848388 1105 66-67 0.3425632648490904 1105 68-69 -0.008995224503067334 1105 70-71 0.19634846385943305 1105 72-73 -0.19392031152544575 1105 74-75 -0.6171663374711294 1105 76 -1.0597697991586976 1106 1 0.05820214882804464 1106 2 -0.36513491903166795 1106 3 -1.2291461768754175 1106 4 -0.0142627597461491 1106 5 -0.34358697445093256 1106 6 -1.0297645853795032 1106 7 0.12619960626425453 1106 8 0.21491079781062794 1106 9 0.6715495162539042 1106 10-11 0.33167897365768084 1106 12-13 -0.29666369810111703 1106 14-15 -0.53069400343092 1106 16-17 0.2665094062147553 1106 18-19 -0.11153284147486531 1106 20-21 -0.06547909940296393 1106 22-23 -0.12037404850095257 1106 24-25 -0.01726941214229427 1106 26-27 -0.0886341076343733 1106 28-29 -0.7131195968871609 1106 30-31 -0.5505082663648047 1106 32-33 0.4624649092768962 1106 34-35 0.9589255086011867 1106 36-37 0.14372604027911962 1106 38-39 -0.1065601057199892 1106 40-41 0.41747276609132555 1106 42-43 -0.06470972469452363 1106 44-45 0.2161374703425345 1106 46-47 1.1101838447335695 1106 48-49 -0.11650913603403978 1106 50-51 -0.5696228039640872 1106 52-53 -0.6013493479391308 1106 54-55 -0.048461552955227916 1106 56-57 0.40794052342610954 1106 58-59 -0.17238228972064462 1106 60-61 0.040612847024611654 1106 62-63 -0.27084950738139923 1106 64-65 -0.047992631184836654 1106 66-67 -0.0254514410332618 1106 68-69 1.0880635990263414 1106 70-71 0.49781905209472654 1106 72-73 -0.10219237034897688 1106 74-75 -1.6309531021770134 1106 76 -0.7737403873939925 1107 1 -2.0214249229951626 1107 2 -1.8837813278714464 1107 3 -0.3187180000798371 1107 4 -0.6964105498013993 1107 5 -0.23944332804209267 1107 6 -1.2314565743297798 1107 7 -1.4186898964981758 1107 8 -0.13370798671975948 1107 9 -1.2642930251825604 1107 10-11 -1.6681829047953585 1107 12-13 -1.017209278211613 1107 14-15 -2.2548998045358957 1107 16-17 -0.8707143506913191 1107 18-19 -0.959081184016302 1107 20-21 -1.2234210883532413 1107 22-23 -1.7656606507108954 1107 24-25 -1.792925489490358 1107 26-27 -1.8379780302863082 1107 28-29 -1.732629265395449 1107 30-31 -1.5604184873592821 1107 32-33 -0.6093410299496256 1107 34-35 -1.1258293256529583 1107 36-37 -2.3053243740855187 1107 38-39 -1.1883798571012036 1107 40-41 -1.0123096924722113 1107 42-43 -1.5083216031475608 1107 44-45 -0.7708618390497328 1107 46-47 -1.3446676746034498 1107 48-49 -1.4188917327191213 1107 50-51 -1.5114908978867376 1107 52-53 -0.9979653700385818 1107 54-55 -0.8565761938392029 1107 56-57 -0.19882743237499767 1107 58-59 -0.8072234499416382 1107 60-61 -0.834525274522349 1107 62-63 -1.6040677394255987 1107 64-65 -1.98711555936163 1107 66-67 -1.1678202255636485 1107 68-69 -1.35475408053167 1107 70-71 -1.6785621633748882 1107 72-73 -1.423169580293731 1107 74-75 -2.194091914331711 1107 76 -1.512483481316643 1108 1 0.5643412020824812 1108 2 0.7450721815600474 1108 3 0.29356092371629927 1108 4 0.20263812782781443 1108 5 0.4590757474425615 1108 6 1.06531677556724 1108 7 0.7585960559683969 1108 8 1.5527806202958345 1108 9 0.9334578002775729 1108 10-11 0.998690807977205 1108 12-13 1.1329590829639713 1108 14-15 1.0189731563323932 1108 16-17 0.27664254230943186 1108 18-19 0.261063312371288 1108 20-21 0.20057119645502652 1108 22-23 -0.1942460899210694 1108 24-25 -0.2549543233848972 1108 26-27 -0.21618588278230533 1108 28-29 0.6646540717518903 1108 30-31 1.048493212925134 1108 32-33 0.747764465489908 1108 34-35 0.3908596801988189 1108 36-37 0.358574413060186 1108 38-39 1.045233533333267 1108 40-41 0.8402353400558233 1108 42-43 1.1585521096250027 1108 44-45 1.52895181945496 1108 46-47 0.8342038151477702 1108 48-49 0.7299035858594536 1108 50-51 0.41563977000041064 1108 52-53 0.73097313726797 1108 54-55 0.5311612281098625 1108 56-57 0.6746564997574716 1108 58-59 -0.4891352483005278 1108 60-61 0.9423879949536058 1108 62-63 0.41812978255942923 1108 64-65 1.0395443510636824 1108 66-67 0.29677489032768634 1108 68-69 1.1214955516890655 1108 70-71 1.5757776319763828 1108 72-73 0.9410953515445115 1108 74-75 1.2031260398348174 1108 76 1.0832862398249432 1109 1 -1.398776378779317 1109 2 -1.2677422809948595 1109 3 -0.8603578333171349 1109 4 -1.1846998763105674 1109 5 -0.941439735187128 1109 6 -2.1257385117598737 1109 7 -1.2319292280915732 1109 8 0.274420000264616 1109 9 -0.21748422607738505 1109 10-11 -0.5543639711275965 1109 12-13 -0.6287572563833237 1109 14-15 -1.59737345128368 1109 16-17 -1.5633218821288004 1109 18-19 -0.5430895899411219 1109 20-21 -0.2925496515502033 1109 22-23 -0.5364975147586222 1109 24-25 -1.1341789213447449 1109 26-27 -1.2697307334012464 1109 28-29 -1.134707020200043 1109 30-31 -0.21465703937094105 1109 32-33 -0.3591800293734124 1109 34-35 -1.3038620680859303 1109 36-37 -1.0182179781257865 1109 38-39 -0.6195394001985157 1109 40-41 -1.0284436449516186 1109 42-43 -1.0601468412589377 1109 44-45 -1.1052007198415161 1109 46-47 -1.6753798055731792 1109 48-49 -1.0172376636413993 1109 50-51 -0.8334073131665392 1109 52-53 -2.0008125381231814 1109 54-55 -0.9391440682926486 1109 56-57 -0.5244214397640761 1109 58-59 -1.0149819829721807 1109 60-61 -0.604785925981524 1109 62-63 -1.1585028816145275 1109 64-65 -0.2999481526572296 1109 66-67 -0.8394468398062678 1109 68-69 -0.7153106341024937 1109 70-71 -1.436383401893007 1109 72-73 -1.9318127691219829 1109 74-75 -0.3968848506432714 1109 76 -1.1687557248173057 1110 1 0.10654552232201908 1110 2 -0.1798939551762473 1110 3 -0.6374293094055368 1110 4 0.3919872402538509 1110 5 -0.5959966130051484 1110 6 0.9082625230542334 1110 7 -0.022896779277914447 1110 8 1.0185252556419506 1110 9 -0.7008902427822399 1110 10-11 -1.4083812673061757 1110 12-13 -0.4639076740047301 1110 14-15 -0.6857391841538103 1110 16-17 0.3868407315159601 1110 18-19 0.637337640452845 1110 20-21 0.09400885240426504 1110 22-23 -0.22974904850095257 1110 24-25 -0.3583085687687948 1110 26-27 -0.649100975104254 1110 28-29 0.4227990778116322 1110 30-31 -0.2313064591358902 1110 32-33 0.2645356924094244 1110 34-35 0.6446559302879287 1110 36-37 0.2503901969056237 1110 38-39 0.37096248464145987 1110 40-41 0.7013959588624097 1110 42-43 0.32678123916089774 1110 44-45 1.3441118679328952 1110 46-47 0.5282937844926039 1110 48-49 0.9519396591466851 1110 50-51 1.2225835213371212 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0.48145255600723047 2116 44-45 1.9365870317460399 2116 46-47 0.29631323069848037 2116 48-49 0.9219777060712211 2116 50-51 1.1814188627025786 2116 52-53 1.4488699503064844 2116 54-55 0.7025252891500351 2116 56-57 1.201361576057689 2116 58-59 0.028823850630232073 2116 60-61 -0.4251766266595993 2116 62-63 0.9429662820922857 2116 64-65 0.898388947762534 2116 66-67 0.9009739975632307 2116 68-69 1.2406951779737128 2116 70-71 -1.3657774391333426 2116 72-73 0.8700115770194436 2116 74-75 0.05166751185807428 2116 76 0.3865666301498685 2117 1 1.2496844497129977 2117 2 0.799688089817888 2117 3 0.9764954160449371 2117 4 0.9672084791919033 2117 5 0.7011032910357962 2117 6 0.1509323172753625 2117 7 -0.140393314089728 2117 8 -0.637301591569905 2117 9 1.6977663304131987 2117 10-11 1.533670124100155 2117 12-13 2.0401172753502124 2117 14-15 1.2077462620558066 2117 16-17 1.0064430345333406 2117 18-19 1.1249162735693865 2117 20-21 1.057197891747478 2117 22-23 0.9826403320300194 2117 24-25 1.6675204108665618 2117 26-27 1.931664564932003 2117 28-29 1.2567365978031049 2117 30-31 0.9732196097413883 2117 32-33 1.7725589358255647 2117 34-35 1.1940748448843728 2117 36-37 0.13827803142956085 2117 38-39 1.282626841182669 2117 40-41 -0.03631595072283389 2117 42-43 0.7953013372523792 2117 44-45 1.4558775145903233 2117 46-47 0.5724349509282618 2117 48-49 0.033878032107555356 2117 50-51 1.4550176827615786 2117 52-53 1.5248674662201545 2117 54-55 0.38964685412441824 2117 56-57 0.3395776915677047 2117 58-59 -0.0111544136144488 2117 60-61 1.8485774487945221 2117 62-63 1.0958540324416077 2117 64-65 0.7461998466912689 2117 66-67 -0.35282975961733243 2117 68-69 0.4026653689378463 2117 70-71 1.7276863087318937 2117 72-73 1.4132113907129664 2117 74-75 1.417138711982279 2117 76 0.7283613825175124 2118 1 1.2740805272064222 2118 2 2.367973189076441 2118 3 1.5741195888903512 2118 4 0.9955908438574532 2118 5 0.6505571696932115 2118 6 2.7196160452511293 2118 7 1.7669653720300182 2118 8 -2.3805846976848706 2118 9 0.8358513180557061 2118 10-11 1.9873096042883134 2118 12-13 2.592018734331319 2118 14-15 2.5040469875600735 2118 16-17 2.4357661629715146 2118 18-19 1.9855955369035136 2118 20-21 -0.11469081111467716 2118 22-23 1.662142843390935 2118 24-25 2.4504670743441928 2118 26-27 2.4546654419151785 2118 28-29 2.3040538265362613 2118 30-31 2.2694804723739317 2118 32-33 2.292729548916533 2118 34-35 2.23485456265524 2118 36-37 1.7186415808196607 2118 38-39 2.620663993379111 2118 40-41 1.3334919102354696 2118 42-43 2.3543781131433157 2118 44-45 2.6531588667389308 2118 46-47 1.5314957816705075 2118 48-49 1.8448084315335258 2118 50-51 2.2467341780179027 2118 52-53 1.099889390799607 2118 54-55 3.0082388974952217 2118 56-57 3.236093676579266 2118 58-59 3.812133476045119 2118 60-61 1.5325047389165043 2118 62-63 2.590073916042023 2118 64-65 2.0822438553016482 2118 66-67 0.09431207248024975 2118 68-69 3.3299554909182376 2118 70-71 1.4782244575001293 2118 72-73 1.4704146116330037 2118 74-75 2.2849534293545197 2118 76 3.5295253783717726 2119 1 1.4730217364569107 2119 2 1.3835764211745172 2119 3 1.3856734107534514 2119 4 0.587863528913644 2119 5 1.5373408606006151 2119 6 1.2230060331771995 2119 7 1.0011996062642545 2119 8 2.2283128596662998 2119 9 0.5701319904807107 2119 10-11 1.4906995922143835 2119 12-13 1.0925759926205387 2119 14-15 2.3171797079092826 2119 16-17 1.3955042515755842 2119 18-19 0.638144993576681 2119 20-21 0.3547528593599232 2119 22-23 2.83210662160214 2119 24-25 1.546454814661832 2119 26-27 1.235012799582119 2119 28-29 1.9527953515664436 2119 30-31 1.5513602903362234 2119 32-33 1.8077290845346283 2119 34-35 1.5880170034465415 2119 36-37 1.2348020712069534 2119 38-39 1.9030081932490859 2119 40-41 1.0347085908335956 2119 42-43 1.6969526464395024 2119 44-45 1.488721233229132 2119 46-47 1.2852804942181066 2119 48-49 1.2927047814917323 2119 50-51 1.950319206345192 2119 52-53 0.4590887963907626 2119 54-55 0.7949018491066298 2119 56-57 1.7891260904364188 2119 58-59 1.8814192566711085 2119 60-61 1.429272640839045 2119 62-63 1.568712348288706 2119 64-65 2.997819224485262 2119 66-67 2.096262476492509 2119 68-69 1.7370326711912938 2119 70-71 1.3872520417854481 2119 72-73 0.905958402846899 2119 74-75 1.2328033411219543 2119 76 1.887470952812194 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 2.0 12 22.0 13 159.0 14 582.0 15 1208.0 16 1644.0 17 1916.0 18 2034.0 19 1911.0 20 1587.0 21 1329.0 22 916.0 23 794.0 24 649.0 25 657.0 26 659.0 27 689.0 28 767.0 29 956.0 30 1208.0 31 1512.0 32 1929.0 33 2570.0 34 3703.0 35 5521.0 36 9245.0 37 10287.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 32.29867632921689 30.30947275012941 9.363676698957333 28.028174221696368 2 16.27522632535762 23.598435462842243 30.978570248085646 29.14776796371449 3 14.213048827515287 32.35213103916852 27.853168555006704 25.581651578309494 4 10.525735901720623 29.122059606662138 32.814881466110876 27.53732302550636 5 10.14194685715335 42.51611363093817 30.1963016692069 17.145637842701582 6 20.68971849349028 38.51111886442514 16.774702976660485 24.024459665424096 7 22.76291385864076 34.379418623868375 19.523660870044253 23.33400664744661 8 21.60603779128487 36.19920304093138 18.484308720641973 23.71045044714178 9 20.801733477789817 21.33977266467121 18.84422571937492 39.014268138164056 10-11 20.678700626182124 27.36103714857594 26.08021007400334 25.88005215123859 12-13 19.583340984630073 24.69930403804837 26.295058486512296 29.422296490809263 14-15 19.000312172907062 26.476853297096792 23.557118460436673 30.96571606955947 16-17 21.586756523495602 28.432524744293662 23.888572635290227 26.092146096920505 18-19 20.215032043630753 27.33716510274161 24.263180123767373 28.18462272986026 20-21 19.297794590227152 27.644747231760842 24.691040637567255 28.366417540444754 22-23 21.444442404098645 27.526305158198213 24.30082450373689 26.72842793396625 24-25 20.462015902455146 30.03470628202068 23.13109425785482 26.372183557669356 26-27 19.16741649374736 30.09897717465156 25.203371467396295 25.530234864204786 28-29 20.55383146335641 28.01751840901996 26.67701121986154 24.75163890776209 30-31 20.21044126558569 27.709936280000736 26.990102282534846 25.089520171878732 32-33 18.183153680885837 30.40839561488881 26.368510935233303 25.03993976899205 34-35 19.3813467506473 29.22581119048056 25.89474264098279 25.498099417889343 36-37 20.011201498429955 27.648419854196888 26.338211800135884 26.00216684723727 38-39 19.621903520208605 26.839524762656776 27.782470573112732 25.756101144021887 40-41 18.80566318379639 26.962557614264465 27.281157610591844 26.9506215913473 42-43 17.470664928292045 26.584277503351267 28.6244192665773 27.320638301779386 44-45 17.5762528233285 26.670584130598453 29.15144058615054 26.601722459922506 46-47 17.8757356789364 28.117855536070074 27.66336433667239 26.343044448321134 48-49 17.30356060745175 29.16796738711277 26.977248104008666 26.551223901426813 50-51 17.488109884863288 28.262665956626332 26.358411223534166 27.89081293497622 52-53 17.0813669500707 27.944065960298953 26.80279853829627 28.171768551334083 54-55 17.097893751032924 27.999155296839707 25.995739757974178 28.907211194153188 56-57 17.585434379418626 28.3039829590319 26.011348403327396 28.099234258222083 58-59 17.855372128468332 27.428980663642875 26.032465982334685 28.683181225554105 60-61 17.36599518886461 26.771581247589843 25.594505756835666 30.26791780670988 62-63 17.79936463631856 27.04060084103054 25.86811612832143 29.291918394329468 64-65 18.11061938777384 27.794406596029898 24.564335163523516 29.53063885267275 66-67 17.62216060377913 29.919018675285088 23.546100593128525 28.912720127807262 68-69 17.418330058578327 29.669280349633652 24.656150724424776 28.256238867363244 70-71 17.65796867253062 30.252309161356667 24.61575187762822 27.473970288484495 72-73 17.80120094753659 29.99338927961511 24.368768018803827 27.83664175404448 74-75 17.698367519327178 30.15131204436528 23.899590502598382 28.250729933709167 76 17.421084525405366 29.149604274932518 23.943661971830984 29.485649227831136 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.5 3 0.5 4 0.0 5 0.5 6 0.5 7 1.0 8 2.0 9 1.0 10 0.5 11 1.5 12 2.0 13 2.0 14 3.0 15 7.0 16 10.5 17 11.0 18 14.5 19 26.0 20 46.0 21 58.0 22 68.0 23 104.0 24 146.0 25 162.0 26 191.5 27 260.5 28 352.0 29 404.0 30 451.0 31 547.0 32 764.0 33 932.0 34 1095.5 35 1307.5 36 1511.0 37 1666.0 38 1877.0 39 2280.0 40 2646.0 41 3001.5 42 3183.0 43 3331.5 44 3557.5 45 3678.5 46 3722.0 47 3666.0 48 3592.5 49 3368.0 50 3161.0 51 3001.0 52 2614.5 53 2207.5 54 2027.0 55 1953.0 56 1638.0 57 1270.5 58 1144.0 59 1001.0 60 766.0 61 584.0 62 494.0 63 411.5 64 313.5 65 240.5 66 161.0 67 139.0 68 105.5 69 58.0 70 43.0 71 42.0 72 35.0 73 22.0 74 18.5 75 21.0 76 15.0 77 10.0 78 9.5 79 8.0 80 9.5 81 9.0 82 5.5 83 4.0 84 4.0 85 4.5 86 3.0 87 1.0 88 2.0 89 3.0 90 3.0 91 3.5 92 4.0 93 3.0 94 1.5 95 1.5 96 2.0 97 3.0 98 4.5 99 4.5 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.6702535945792093 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 9.181556090126153E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 54457.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.34096626696292 #Duplication Level Percentage of deduplicated Percentage of total 1 83.89098693863805 64.04319003984796 2 9.268034541649628 14.15061424610243 3 3.499867702595435 8.015498466680134 4 1.476920116421716 4.5099803514699675 5 0.7817573906141005 2.9840057292910003 6 0.44259495345536765 2.027287584699855 7 0.20686503259326966 1.105459353251189 8 0.1635677001900272 0.9989533026057256 9 0.1226757751425204 0.842866849073581 >10 0.14672984869987732 1.3221440769781663 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0018363112180252309 0.0 9 0.0 0.0 0.0 0.0036726224360504617 0.0 10 0.0 0.0 0.0 0.005508933654075693 0.0 11 0.0 0.0 0.0 0.009181556090126155 0.0 12 0.0 0.0 0.0 0.009181556090126155 0.0 13 0.0 0.0 0.0 0.011017867308151385 0.0 14 0.0 0.0 0.0 0.011017867308151385 0.0 15 0.0 0.0 0.0 0.011017867308151385 0.0 16 0.0 0.0 0.0 0.011017867308151385 0.0 17 0.0 0.0 0.0 0.011017867308151385 0.0 18 0.0 0.0 0.0 0.012854178526176617 0.0 19 0.0 0.0 0.0 0.016526800962227077 0.0 20 0.0 0.0 0.0 0.02203573461630277 0.0 21 0.0 0.0 0.0 0.023872045834328 0.0 22 0.0 0.0 0.0 0.027544668270378464 0.0 23 0.0 0.0 0.0 0.034889913142479384 0.0 24 0.0 0.0 0.0 0.04039884679655508 0.0 25 0.0 0.0 0.0 0.04039884679655508 0.0 26 0.0 0.0 0.0 0.04223515801458031 0.0 27 0.0 0.0 0.0 0.06243458141285785 0.0 28 0.0 0.0 0.0 0.08447031602916062 0.0 29 0.0 0.0 0.0 0.12303285160769047 0.0 30 0.0 0.0 0.0 0.167104320840296 0.0 31 0.0 0.0 0.0 0.21852103494500247 0.0 32 0.0 0.0 0.0 0.24422939199735572 0.0 33 0.0 0.0 0.0 0.28279192757588556 0.0 34 0.0 0.0 0.0 0.33971757533466773 0.0 35 0.0 0.0 0.0 0.43704206989000494 0.0 36 0.0 0.0 0.0 0.5876195897680739 0.0 37 0.0 0.0 0.0 0.7969590686229502 0.0 38 0.0 0.0 0.0 1.0926051747250123 0.0 39 0.0 0.0 0.0 1.4598674183300586 0.0 40 0.0 0.0 0.0 1.8675285087316598 0.0 41 0.0 0.0 0.0 2.258662798171034 0.0 42 0.0 0.0 0.0 2.6277613529941055 0.0 43 0.0 0.0 0.0 3.0335861321776814 0.0 44 0.0 0.0 0.0 3.4100299318728537 0.0 45 0.0 0.0 0.0 3.769946930605799 0.0 46 0.0 0.0 0.0 4.25106046972841 0.0 47 0.0 0.0 0.0 4.7009567181445915 0.0 48 0.0 0.0 0.0 5.169216078741025 0.0 49 0.0 0.0 0.0 5.584222414014727 0.0 50 0.0 0.0 0.0 6.021264483904732 0.0 51 0.0 0.0 0.0 6.463815487448813 0.0 52 0.0 0.0 0.0 6.911875424646969 0.0 53 0.0 0.0 0.0 7.345244872100924 0.0 54 0.0 0.0 0.0 7.804322676607232 0.0 55 0.0 0.0 0.0 8.237692124061185 0.0 56 0.0 0.0 0.0 8.656371081770939 0.0 57 0.0 0.0 0.0 9.003433901977708 0.0 58 0.0 0.0 0.0 9.412931303597334 0.0 59 0.0 0.0 0.0 9.837119194961161 0.0 60 0.0 0.0 0.0 10.255798152670915 0.0 61 0.0 0.0 0.0 10.691003911342895 0.0 62 0.0 0.0 0.0 11.09682869052647 0.0 63 0.0 0.0 0.0 11.495308224837945 0.0 64 0.0 0.0 0.0 11.849716289916815 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTGTAT 20 0.006769122 52.786705 1 GCCTCAG 20 0.006769122 52.786705 1 CGTCAGA 20 0.006769122 52.786705 1 CAAACAG 20 0.0069188536 52.49587 2 GGTCGTT 20 0.0069188536 52.49587 46 AGCCCAT 20 0.0069188536 52.49587 6 TGTATAA 20 0.0069188536 52.49587 10 GTCAGAT 20 0.0069188536 52.49587 2 TCAGATG 20 0.0069188536 52.49587 3 TCGTCTT 20 0.0069188536 52.49587 14 ATCATGC 20 0.0069188536 52.49587 8 GTGTATA 30 5.832188E-4 46.662994 9 GCCGCCG 35 0.0012457465 39.996853 17 AGGTAAT 45 9.091142E-5 38.885826 53 CCGCCGA 45 9.091142E-5 38.885826 18 ATCTCTG 40 0.002400244 34.997246 39 GGTAATA 50 1.6900005E-4 34.997242 54 TCTCTGT 50 0.007153895 27.997793 40 TGCCGCC 50 0.007153895 27.997793 16 TAGGAGG 80 0.002636174 21.87328 4 >>END_MODULE