FastQCFastQC Report
Wed 25 May 2016
SRR1780454_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780454_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54457
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTCTT30355.573204546706576Illumina PCR Primer Index 2 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGT27475.044346915915309No Hit
CTTTACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTC9931.8234570394990541No Hit
CTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTC4660.8557210275997577No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCG3080.5655838551517711No Hit
TATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTCT2590.47560460546853484No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCC1460.2681014378316837No Hit
CTTAATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCG1430.26259250417760804No Hit
CTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATATCGTATGCCGT1300.23872045834328004No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCC1290.2368841471252548No Hit
CTTATAACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCG1140.20933947885487633No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCC1100.20199423398277538No Hit
ATACACATCTCCGAGCCCACGAGACCCGTTTGTAACTCGTATGCCGTCTT850.15608645353214465No Hit
CTTAACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTC720.13221440769781662No Hit
TTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTC710.1303780964797914No Hit
ATACACATCTCCGAGCCCACGAGACCCGTTTGTACCTCGTATGCCGTCTT700.12854178526176616No Hit
CTTATACACATCTCCGAGCCCACGAGACCCGTTTGTACCTCGTATGCCGT660.12119654038966524No Hit
CTTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCG620.11385129551756432No Hit
CTTATACACATCTCCGAGCCCACGAGACCCGTTTGTAACTCGTATGCCGT570.10466973942743817No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTACAC950.070.000012
TCCATAT150.002213508170.012
ACGTTTG150.002213508170.029
TCTTAAT207.8610894E-570.01
CACGTTT150.002213508170.028
CTTATAA352.9232433E-760.01
GTCGCCT200.00691670152.539
ACACGTT200.00691670152.527
CTGTATC200.00691670152.530
GGGCTTG200.00691670152.51
ATCGTAT407.3523006E-752.539
AATTGGT200.00691670152.560
AGACACG200.00691670152.525
CGAGACA200.00691670152.523
CTTTACA1200.052.51
GTCCATA200.00691670152.511
AAAGCGA200.00691670152.520
TTACACA1300.051.153853
TTGTATA305.829927E-446.66666433
ATATCGT305.829927E-446.66666437
Produced by FastQC (version 0.11.5)