##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780454_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 54457 Sequences flagged as poor quality 0 Sequence length 76 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.891988174155756 34.0 34.0 34.0 31.0 34.0 2 33.244725196026224 34.0 34.0 34.0 32.0 34.0 3 33.4849330664561 34.0 34.0 34.0 34.0 34.0 4 33.49615292799824 34.0 34.0 34.0 34.0 34.0 5 33.49095616725123 34.0 34.0 34.0 34.0 34.0 6 37.22632535762161 38.0 37.0 38.0 36.0 38.0 7 36.81660759865582 38.0 37.0 38.0 35.0 38.0 8 36.83203261288723 38.0 37.0 38.0 35.0 38.0 9 36.65080705878032 38.0 37.0 38.0 34.0 38.0 10-11 36.34100299318729 38.0 37.0 38.0 33.0 38.0 12-13 36.16364287419432 38.0 37.0 38.0 34.0 38.0 14-15 36.322704151899664 38.0 37.0 38.0 34.0 38.0 16-17 36.43895183355675 38.0 37.0 38.0 34.0 38.0 18-19 36.46262188515709 38.0 37.0 38.0 34.0 38.0 20-21 36.21749270066291 38.0 37.0 38.0 33.5 38.0 22-23 36.21342527131498 38.0 36.5 38.0 32.5 38.0 24-25 35.411572433295994 38.0 35.5 38.0 29.0 38.0 26-27 35.68832289696458 38.0 36.0 38.0 31.0 38.0 28-29 35.35596892961419 38.0 36.0 38.0 29.0 38.0 30-31 35.0079971353545 37.0 35.5 38.0 29.0 38.0 32-33 35.44127476724755 38.0 36.0 38.0 31.0 38.0 34-35 35.344528710725896 38.0 36.0 38.0 31.0 38.0 36-37 35.5395174174119 38.0 36.0 38.0 31.0 38.0 38-39 34.9245826982757 37.5 35.5 38.0 28.5 38.0 40-41 34.83688965605891 37.0 34.5 38.0 27.0 38.0 42-43 35.494536974126376 37.5 36.0 38.0 29.5 38.0 44-45 35.74922415851039 38.0 36.5 38.0 31.5 38.0 46-47 35.94176138972033 38.0 37.0 38.0 32.5 38.0 48-49 35.7052261417265 38.0 36.5 38.0 31.5 38.0 50-51 35.81634133352921 38.0 36.5 38.0 31.0 38.0 52-53 35.62522724351323 38.0 36.0 38.0 31.0 38.0 54-55 35.87346897552197 38.0 37.0 38.0 32.0 38.0 56-57 35.86947499862276 38.0 37.0 38.0 32.0 38.0 58-59 35.90050865820739 38.0 37.0 38.0 32.0 38.0 60-61 35.617781001524136 38.0 36.5 38.0 31.0 38.0 62-63 35.97196870925684 38.0 37.0 38.0 33.0 38.0 64-65 35.99044200011018 38.0 37.0 38.0 33.0 38.0 66-67 34.981517527590576 38.0 35.5 38.0 28.0 38.0 68-69 33.41632847935068 37.0 33.5 38.0 21.5 38.0 70-71 32.432708375415466 37.0 31.0 38.0 16.0 38.0 72-73 31.457415942853995 37.0 26.0 38.0 11.0 38.0 74-75 31.817718566942723 37.0 27.5 38.0 11.0 38.0 76 31.512128835595057 37.0 27.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.15308186198394225 1101 2 0.6484142377660262 1101 3 0.18672053752131745 1101 4 0.40258305143290585 1101 5 0.2665880864665411 1101 6 -0.7201499263583457 1101 7 0.23576589953294302 1101 8 0.6588940019468126 1101 9 0.3216075849736981 1101 10-11 0.2953076869554181 1101 12-13 0.9018748997035928 1101 14-15 0.5904043896132549 1101 16-17 -0.13070444550378113 1101 18-19 -0.4267006479505113 1101 20-21 -0.38985023057070833 1101 22-23 0.701845804906938 1101 24-25 -1.4688199115573823 1101 26-27 -0.7495127550239999 1101 28-29 0.2559301178997657 1101 30-31 1.4589494238970389 1101 32-33 -0.04826053040073219 1101 34-35 0.2560533872083326 1101 36-37 0.5186358391276258 1101 38-39 -0.004435591684611495 1101 40-41 1.3154230308012131 1101 42-43 0.40809461459972596 1101 44-45 0.5496661971615353 1101 46-47 0.799317632157134 1101 48-49 -2.081878120675505 1101 50-51 -2.1512447506010446 1101 52-53 -2.7288753963961767 1101 54-55 -1.9195584246193107 1101 56-57 -1.434649967399423 1101 58-59 -2.0453418326324737 1101 60-61 -2.8800927613587533 1101 62-63 -1.4876972983534529 1101 64-65 -1.7331413788875736 1101 66-67 -2.5730804368311126 1101 68-69 -2.606863651411768 1101 70-71 -2.4771533789432283 1101 72-73 -3.6641024353267504 1101 74-75 -3.5979934629607087 1101 76 -1.5863522759416426 1102 1 -0.45188054403109135 1102 2 0.1280132352597576 1102 3 0.30025437210778705 1102 4 0.46544019429005346 1102 5 0.31571089348408776 1102 6 0.3003513267744822 1102 7 0.20498895717705068 1102 8 0.7518764580871675 1102 9 0.48346222156517626 1102 10-11 0.9867111957273451 1102 12-13 1.0716743984504689 1102 14-15 0.15368759763330786 1102 16-17 0.5938569580049844 1102 18-19 0.05272291094674131 1102 20-21 0.8131572882262859 1102 22-23 0.7703671081650825 1102 24-25 -4.131025425341843 1102 26-27 -0.9381593715653622 1102 28-29 0.32485242366418277 1102 30-31 1.9856912785336363 1102 32-33 0.3719650335090421 1102 34-35 0.8011411065065701 1102 36-37 -2.1470283212733747 1102 38-39 -0.10483659419087843 1102 40-41 1.265899221277408 1102 42-43 0.7893978727450843 1102 44-45 1.1469343675876047 1102 46-47 1.2462349253902119 1102 48-49 -3.380624987843426 1102 50-51 -0.7492146754130715 1102 52-53 0.40305442816523396 1102 54-55 0.9395393197415913 1102 56-57 0.7457259724502023 1102 58-59 0.0668135558387064 1102 60-61 -5.253100280155746 1102 62-63 -2.098248676799564 1102 64-65 0.6564074932928747 1102 66-67 1.642408284973392 1102 68-69 -0.7119263080533713 1102 70-71 -1.2188325769382153 1102 72-73 -4.64781170850971 1102 74-75 -1.8710511070709863 1102 76 -0.39341994511457656 1103 1 0.7124981541937601 1103 2 0.5254885804274139 1103 3 -0.0920533201999092 1103 4 0.2910023244675699 1103 5 0.18592785600873896 1103 6 0.25155448259302204 1103 7 0.5364287993861225 1103 8 0.7769257677518553 1103 9 0.5447245489813497 1103 10-11 0.5421155349778388 1103 12-13 -0.43473585795978664 1103 14-15 -0.4523281814594 1103 16-17 0.2668352617525258 1103 18-19 0.8880878024654777 1103 20-21 -0.02707939816424698 1103 22-23 0.661649159447137 1103 24-25 -3.1064494884582103 1103 26-27 -0.08685759641743118 1103 28-29 0.08717983983774502 1103 30-31 1.2405039018077986 1103 32-33 1.4354561577693943 1103 34-35 0.7931134930943458 1103 36-37 -0.42552930746666817 1103 38-39 0.5590292835211557 1103 40-41 0.36487358025176775 1103 42-43 -0.12878753159415623 1103 44-45 1.352397878435724 1103 46-47 1.4271816709523435 1103 48-49 -1.7620845539183776 1103 50-51 -0.25637443872668086 1103 52-53 0.6041984123861397 1103 54-55 0.29646239666467267 1103 56-57 0.8961993452312669 1103 58-59 -0.26701287414157093 1103 60-61 -3.852331049386514 1103 62-63 -1.4636727399159426 1103 64-65 -0.09406587948819123 1103 66-67 -1.2592485197603267 1103 68-69 0.1747798063450503 1103 70-71 0.6134948392353508 1103 72-73 -3.3174369550580316 1103 74-75 -1.2875205350788725 1103 76 -2.821703968783215 1104 1 0.296953811797124 1104 2 0.21398460540290642 1104 3 0.5266347402059424 1104 4 -0.12327146215166351 1104 5 -0.509646979726547 1104 6 0.24313251286855575 1104 7 0.8350094070748 1104 8 0.7005472147333833 1104 9 1.0655072113402255 1104 10-11 -0.2731456549884044 1104 12-13 0.7705496540741947 1104 14-15 0.7799657162427138 1104 16-17 0.3308712729334147 1104 18-19 0.8744202524600269 1104 20-21 -0.1101555952092994 1104 22-23 0.31691107544524755 1104 24-25 0.6939643497092831 1104 26-27 0.9114725302751339 1104 28-29 1.8453023214146924 1104 30-31 1.1761411762841476 1104 32-33 0.4192656470059788 1104 34-35 0.9097505134595352 1104 36-37 1.4083909016305114 1104 38-39 1.6347544078541105 1104 40-41 1.7185781578827246 1104 42-43 0.2279663799025542 1104 44-45 0.4896542039884224 1104 46-47 1.2257441278442016 1104 48-49 0.11467153567395627 1104 50-51 0.42458900556852086 1104 52-53 0.7199460437071536 1104 54-55 0.4005209148336135 1104 56-57 0.2218609417753612 1104 58-59 -0.533902190582566 1104 60-61 0.5119917443841331 1104 62-63 0.46864293874235585 1104 64-65 1.2305792724339781 1104 66-67 0.4536966285316808 1104 68-69 2.4498732829486727 1104 70-71 2.2213105723460345 1104 72-73 0.771411195375773 1104 74-75 0.673836418491387 1104 76 0.07124263157254163 1105 1 0.7210606324394959 1105 2 0.5331112744754449 1105 3 0.30613672504895817 1105 4 -0.30750098218053523 1105 5 -0.3470342045551291 1105 6 0.13133171893134943 1105 7 0.605381114039794 1105 8 -0.6442019732853836 1105 9 0.8791484960749827 1105 10-11 0.5547504114136146 1105 12-13 -0.47930599370639726 1105 14-15 0.5399621074372263 1105 16-17 0.37719029133832294 1105 18-19 0.7182131070251714 1105 20-21 0.43080434704981485 1105 22-23 0.6417396571846865 1105 24-25 0.9264255550503151 1105 26-27 0.6594876872581708 1105 28-29 1.1258328158210418 1105 30-31 1.250789317749323 1105 32-33 1.415494445273751 1105 34-35 -0.01081967780715587 1105 36-37 0.936305012059961 1105 38-39 -0.02267973144577695 1105 40-41 0.7608011820617264 1105 42-43 0.43724100999999393 1105 44-45 0.8990912303327008 1105 46-47 1.1485878665666789 1105 48-49 0.055649521960496884 1105 50-51 1.0401970892928034 1105 52-53 0.6234465850279776 1105 54-55 1.1577746138592389 1105 56-57 0.47749067833255765 1105 58-59 0.5413233597602698 1105 60-61 1.1316056021971974 1105 62-63 0.07214348006318261 1105 64-65 0.9187604344693412 1105 66-67 0.6194671085028105 1105 68-69 0.4867011429270178 1105 70-71 0.8845007563951199 1105 72-73 0.25905103658833184 1105 74-75 -0.8396785580513786 1105 76 0.24540358429718978 1106 1 -1.0381305440310946 1106 2 0.4338465685930899 1106 3 0.4752543721077842 1106 4 0.20794019429005317 1106 5 0.20737756015075348 1106 6 0.288684660107819 1106 7 -0.30417770948961476 1106 8 0.6352097914205004 1106 9 -0.9094544451014883 1106 10-11 0.3587945290606811 1106 12-13 1.4977160651171317 1106 14-15 0.10264593096663077 1106 16-17 0.34594029133831583 1106 18-19 0.5093895776134048 1106 20-21 0.17878228822628728 1106 22-23 0.6707837748317473 1106 24-25 -0.5051920920085067 1106 26-27 1.076215628434639 1106 28-29 0.573810756997517 1106 30-31 0.573399611866968 1106 32-33 -0.0017849664909590501 1106 34-35 1.0400994398399135 1106 36-37 1.3019300120599624 1106 38-39 0.4403717391424564 1106 40-41 0.9006908879440729 1106 42-43 1.1304395394117535 1106 44-45 0.9090177009209341 1106 46-47 1.0381099253902093 1106 48-49 1.45270834548991 1106 50-51 0.02016032458692507 1106 52-53 0.8482627614985603 1106 54-55 -0.10233568025840611 1106 56-57 0.29072597245020404 1106 58-59 0.915771889172035 1106 60-61 0.8512747198442554 1106 62-63 0.9875846565337625 1106 64-65 -1.3260925067071199 1106 66-67 0.6224082849733961 1106 68-69 1.754532025279957 1106 70-71 -0.05612424360488433 1106 72-73 0.15114662482362462 1106 74-75 -0.6450094404043192 1106 76 -0.3134199451145747 1107 1 1.0410476327644886 1107 2 -0.23942691206988798 1107 3 0.3405858638204933 1107 4 0.48466671362707103 1107 5 0.2895598805927406 1107 6 0.5208669805498047 1107 7 0.8439576496264038 1107 8 0.4283037140724346 1107 9 0.0763535659482315 1107 10-11 0.7535804406628941 1107 12-13 -0.23541584096021495 1107 14-15 0.3861922016848638 1107 16-17 0.648737252664283 1107 18-19 0.694472450541582 1107 20-21 0.9568036970660643 1107 22-23 0.5146028356052312 1107 24-25 0.44087144390309163 1107 26-27 -0.21092180250458625 1107 28-29 0.7493459779919931 1107 30-31 0.3315660483310552 1107 32-33 0.9811915528460631 1107 34-35 0.08303797575150895 1107 36-37 1.3818851225572004 1107 38-39 0.4972260209104107 1107 40-41 0.9626211365628592 1107 42-43 1.1181639869255662 1107 44-45 0.8538036125231514 1107 46-47 0.9640249806388326 1107 48-49 1.2306088979760972 1107 50-51 1.3210063190620644 1107 52-53 0.6830175957527018 1107 54-55 0.827274126371428 1107 56-57 1.0858917183065557 1107 58-59 0.91810269027701 1107 60-61 1.345076515424367 1107 62-63 1.1388968112298983 1107 64-65 0.9096119131823812 1107 66-67 -0.3333928199989842 1107 68-69 0.13510868273852594 1107 70-71 -1.3900848789639966 1107 72-73 -0.9470750602592481 1107 74-75 -2.798030849244096 1107 76 -1.4797182876560129 1108 1 -0.14549001148671437 1108 2 0.5195714206641 1108 3 0.055875673882930244 1108 4 0.40934551973384004 1108 5 0.24509945364187757 1108 6 -0.27507273634188323 1108 7 -0.3511451651109141 1108 8 -0.3650860665676703 1108 9 -0.06474290663994964 1108 10-11 0.14270725095416736 1108 12-13 1.1362700591999726 1108 14-15 0.4885002209074685 1108 16-17 0.00943881204837993 1108 18-19 0.4857209385601493 1108 20-21 1.0409679391138624 1108 22-23 0.25632371566015166 1108 24-25 0.9556806890565852 1108 26-27 -0.29691676801505906 1108 28-29 -0.49861897673029887 1108 30-31 0.17245656452968916 1108 32-33 0.9413733175327081 1108 34-35 0.7664862741594405 1108 36-37 1.6247665505214925 1108 38-39 1.1418695202075426 1108 40-41 1.0128025743346072 1108 42-43 -0.5015685966829153 1108 44-45 0.8849422571339574 1108 46-47 0.9774775289405042 1108 48-49 1.2024124875017463 1108 50-51 0.5335663896756841 1108 52-53 -0.05260632134167054 1108 54-55 0.14261624281851226 1108 56-57 0.49974964108925235 1108 58-59 0.3217445223081299 1108 60-61 1.1595032701401067 1108 62-63 1.3173923488414587 1108 64-65 0.7254607477307431 1108 66-67 1.4803964506538705 1108 68-69 2.269454362558072 1108 70-71 0.3501812297678981 1108 72-73 0.2831547609183005 1108 74-75 0.47283449450692316 1108 76 1.3853966229327597 1109 1 -1.230653550166064 1109 2 0.14649994282622458 1109 3 0.04810713284398105 1109 4 0.15280215748023807 1109 5 -0.1967635441437281 1109 6 -0.1985852785424882 1109 7 -0.5514936604098608 1109 8 0.20974966872110912 1109 9 0.31090598434636263 1109 10-11 0.2943390075882846 1109 12-13 0.37791161726437394 1109 14-15 0.6818062070402533 1109 16-17 0.5087853833628628 1109 18-19 0.3406779211716895 1109 20-21 0.4910713986557269 1109 22-23 0.28623622881947597 1109 24-25 -0.9931522147078979 1109 26-27 -0.46582808322179403 1109 28-29 -1.507458414781631 1109 30-31 -0.5263128114459192 1109 32-33 0.1585294506869559 1109 34-35 -0.5841145172153048 1109 36-37 0.5801700427348067 1109 38-39 0.4537728127620895 1109 40-41 -0.35810895868169723 1109 42-43 0.3629357050559321 1109 44-45 0.42523702607431346 1109 46-47 0.5202226554515619 1109 48-49 0.4275549712567752 1109 50-51 -0.6091533257198236 1109 52-53 -0.3260662262315037 1109 54-55 -1.3326079195222107 1109 56-57 -0.5051329232553172 1109 58-59 -0.5676445218709034 1109 60-61 0.3739549345681752 1109 62-63 0.12508465653376533 1109 64-65 0.7581866343971697 1109 66-67 0.4813039904948724 1109 68-69 -0.4182248765605294 1109 70-71 -0.6866648877766615 1109 72-73 -0.9647851236426312 1109 74-75 -1.4979427226129083 1109 76 -0.640413810145251 1110 1 -0.5668203030672387 1110 2 -0.2268462024912452 1110 3 0.08218208295115659 1110 4 -0.08708992619187939 1110 5 0.46189563243991216 1110 6 0.04260032275841752 1110 7 0.3908524109923164 1110 8 0.6129206347939942 1110 9 0.9329401332117655 1110 10-11 -0.665377157686315 1110 12-13 0.37990733017737455 1110 14-15 0.8815992442196432 1110 16-17 0.47297342386844576 1110 18-19 -0.294827289856471 1110 20-21 0.918578974973272 1110 22-23 -0.9488698396260844 1110 24-25 -0.7978125739362198 1110 26-27 1.0267803874707866 1110 28-29 0.6611978051902838 1110 30-31 -0.013987436325805902 1110 32-33 0.8130493708584368 1110 34-35 1.022064801285687 1110 36-37 1.860401397602125 1110 38-39 1.147412401793062 1110 40-41 -1.815385919284843 1110 42-43 0.1924500815804322 1110 44-45 0.8113520382703285 1110 46-47 0.433704804908281 1110 48-49 1.502708345489907 1110 50-51 -0.13439539830464042 1110 52-53 0.5390383639082046 1110 54-55 0.0026718498620752484 1110 56-57 0.908497056787553 1110 58-59 -0.8558772072135099 1110 60-61 -0.24273883437261645 1110 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48-49 0.3623574682969206 2116 50-51 -1.238337482430623 2116 52-53 0.003580743954699983 2116 54-55 -0.3594080486794624 2116 56-57 0.36046281455546136 2116 58-59 -2.8103794266174376 2116 60-61 -2.1752055433136377 2116 62-63 0.30929518284955293 2116 64-65 -2.172364436531687 2116 66-67 -1.1363636448511656 2116 68-69 -1.8117508694568798 2116 70-71 1.5178340897284457 2116 72-73 0.29078478271836516 2116 74-75 0.4552646824026958 2116 76 -0.5625427521321207 2117 1 0.4247566241105005 2117 2 0.44081559514176405 2117 3 0.060431363258224735 2117 4 -0.02978104464800424 2117 5 0.14874924156668357 2117 6 0.15881740347064977 2117 7 0.8512426444926859 2117 8 0.1144133312435045 2117 9 -1.1341778964289233 2117 10-11 -0.358628037311 2117 12-13 -0.9806854835554333 2117 14-15 0.35648995751530776 2117 16-17 -0.7028982042369023 2117 18-19 -1.3180440507051827 2117 20-21 0.3352811820315935 2117 22-23 0.17083908456626773 2117 24-25 -2.207957578734174 2117 26-27 -2.0311682211228828 2117 28-29 -1.523230172206027 2117 30-31 -0.7410638837082502 2117 32-33 -2.272194258526355 2117 34-35 0.20412045753902675 2117 36-37 -1.7013056074090684 2117 38-39 -0.008494411300020488 2117 40-41 0.1592915516608855 2117 42-43 -0.24906820395107587 2117 44-45 -0.36459955571623226 2117 46-47 -1.5576588799195221 2117 48-49 -1.5039288226516874 2117 50-51 0.014767625471876045 2117 52-53 0.017980681852549196 2117 54-55 -0.6388677599044215 2117 56-57 -0.6426811071958127 2117 58-59 -0.9693221373766363 2117 60-61 -0.6361976252884887 2117 62-63 -0.6050038390414514 2117 64-65 -1.7896987013973913 2117 66-67 -1.4705120690089046 2117 68-69 0.18370791023571087 2117 70-71 -0.15178242944558917 2117 72-73 0.8049906513722966 2117 74-75 0.5845536126930249 2117 76 0.2768455416110882 2118 1 -0.27332198547253483 2118 2 0.32585107309759564 2118 3 -0.507853736000321 2118 4 0.05895370780356757 2118 5 0.07787305564625058 2118 6 -0.06018921376605846 2118 7 0.5556195878076835 2118 8 0.44647105268175835 2118 9 -2.2968981387951857 2118 10-11 -1.5351806961645451 2118 12-13 -2.1738210970450282 2118 14-15 0.12440831835402832 2118 16-17 -2.63497187082384 2118 18-19 -0.7293491611253273 2118 20-21 -1.0877436126746147 2118 22-23 0.6148716126695888 2118 24-25 0.6489745746581619 2118 26-27 -2.609060272466259 2118 28-29 -1.1661385673268114 2118 30-31 -0.8713357484933937 2118 32-33 -0.5796565881125773 2118 34-35 -0.7268769115114466 2118 36-37 -3.1569382311832825 2118 38-39 -1.146818576172862 2118 40-41 -0.5076142922361058 2118 42-43 0.5014699448171598 2118 44-45 -2.2592818486286177 2118 46-47 0.1923610515163361 2118 48-49 0.2076633004448638 2118 50-51 -0.7581335943319942 2118 52-53 -1.4479365628257597 2118 54-55 -1.5822624820602087 2118 56-57 0.6346448913691205 2118 58-59 -0.8318350928099463 2118 60-61 -0.9659831630386293 2118 62-63 -1.5911315596824522 2118 64-65 -0.3009798940945103 2118 66-67 0.1449308074959177 2118 68-69 -0.7130073891344537 2118 70-71 0.7971133690077252 2118 72-73 2.640476579778582 2118 74-75 1.989759703739825 2118 76 2.334327802633169 2119 1 0.36399574462870277 2119 2 -2.0858699262522684 2119 3 -0.1729415041808764 2119 4 -0.5267247541635598 2119 5 -0.634117285210074 2119 6 -1.1244596697890898 2119 7 -1.2468323486648742 2119 8 0.17490051306997856 2119 9 -0.02588485747262581 2119 10-11 0.08695174555552398 2119 12-13 -0.5477414091096691 2119 14-15 -0.25782442985810405 2119 16-17 -0.5862117705173446 2119 18-19 -0.6349403192938112 2119 20-21 -1.3311726086809301 2119 22-23 -0.11471880248784316 2119 24-25 0.8122048152079842 2119 26-27 0.7969952676099012 2119 28-29 0.10148472606967829 2119 30-31 0.6900555397020156 2119 32-33 0.1295938994884267 2119 34-35 0.4852991821079442 2119 36-37 -0.07864344154828729 2119 38-39 -1.2232558381771383 2119 40-41 -1.3944444213342777 2119 42-43 -0.07403855337175003 2119 44-45 0.5686182163848557 2119 46-47 0.05077100786443367 2119 48-49 -0.8761576338915376 2119 50-51 0.382434809123005 2119 52-53 -0.05144729004783244 2119 54-55 0.08551870118488836 2119 56-57 -2.340975058477632 2119 58-59 -0.14366754381765645 2119 60-61 0.1230852868545611 2119 62-63 -1.0192452403734578 2119 64-65 -0.4909120943359895 2119 66-67 0.6584907592001983 2119 68-69 0.9952214582696541 2119 70-71 0.282438900725019 2119 72-73 1.0721195629679592 2119 74-75 1.087883600832793 2119 76 1.2051367559163495 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 1.0 19 1.0 20 0.0 21 1.0 22 9.0 23 14.0 24 38.0 25 110.0 26 182.0 27 352.0 28 578.0 29 944.0 30 1501.0 31 2186.0 32 3178.0 33 4542.0 34 6502.0 35 7885.0 36 10509.0 37 15924.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 32.92506013919239 23.420313274693797 10.641423508456214 33.0132030776576 2 15.388288007051434 18.016049360045542 46.31911416346842 20.276548469434598 3 14.595001560864535 27.60343022935527 37.730686596764414 20.07088161301577 4 10.60836990653176 23.8206291202233 36.123914280992345 29.447086692252604 5 10.024422939199736 41.032374166773785 36.71520649319647 12.227996400830014 6 20.102098903722204 39.895697522816164 17.132783664175406 22.869419909286226 7 20.219622821675816 38.055713682354884 19.220669519070093 22.503993976899206 8 18.16295425748756 36.44343243292874 23.793084451952918 21.600528857630792 9 17.902198064527976 19.4906072681198 18.372293736342435 44.23490093100978 10-11 18.76342802578181 24.805810088693832 31.250344308353377 25.180417577170978 12-13 21.842003782801108 18.705584222414014 28.35815413996364 31.094257854821233 14-15 22.050425106046973 23.136603191508897 21.443524248489634 33.3694474539545 16-17 24.646051012725636 27.09109939952623 20.141579594909746 28.12126999283839 18-19 22.461758818884626 26.643039462328076 20.622693134032353 30.27250858475494 20-21 21.359053932460473 23.814202030960207 20.38213636447105 34.44460767210827 22-23 22.787704060084103 30.562645757202933 20.93302972987862 25.71662045283435 24-25 24.478028536276327 31.90039847953431 20.383972675689076 23.237600308500284 26-27 22.415851038433992 28.531685550067028 20.699818205189416 28.352645206309568 28-29 21.853021650109262 22.826266595662634 25.14093688598344 30.179774868244667 30-31 22.7509778357236 20.960574398149 31.78379271718971 24.504655048937693 32-33 22.668343830912463 21.406798024129127 37.90054538443176 18.024312760526655 34-35 22.660998586040364 25.239179536147788 34.741171933819345 17.358649943992507 36-37 18.922268946140992 26.671502286207467 32.47240942394917 21.93381934370237 38-39 20.722772095414733 23.32390693574747 28.264502267844353 27.688818700993444 40-41 18.807499495014415 24.449565712396936 31.610261307086322 25.132673485502323 42-43 25.3640486989735 22.914409534127845 32.29795985823677 19.42358190866188 44-45 18.94981361441137 25.357621609710414 29.30569072846466 26.386874047413556 46-47 25.26488789320014 21.215821657454505 27.647501698587877 25.871788750757478 48-49 22.436968617441284 20.742971518813007 28.519749527149862 28.300310336595846 50-51 17.395376168353014 21.07350753805755 33.334557540812014 28.196558752777424 52-53 19.262904677084673 20.84855941384946 37.57919092127734 22.309344987788528 54-55 17.669904695447787 20.585048754062836 34.06632756119507 27.678718989294303 56-57 23.6682152891272 20.881613015773915 30.466239418256606 24.983932276842282 58-59 18.056448206842095 28.948528196558755 32.48526360247534 20.509759994123804 60-61 20.1966689314505 37.14123069577832 23.68198762326239 18.980112749508788 62-63 15.485612501606772 43.15698624602898 21.651027416126485 19.706373836237763 64-65 14.84933066456103 44.50667499127752 20.776943276346476 19.867051067814973 66-67 15.141304148227041 43.87498393227684 20.678700626182124 20.30501129331399 68-69 15.413078208494776 41.20406926565914 21.320491396881945 22.062361128964135 70-71 15.633435554657805 38.76912059055769 21.776814734561214 23.8206291202233 72-73 15.866647079347008 35.22871256220505 22.3580072350662 26.546633123381753 74-75 16.100776759645225 34.54652294470867 22.34148043410397 27.011219861542134 76 16.271553702921572 35.290228988008884 22.008189948032392 26.43002736103715 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 1.0 5 0.5 6 0.5 7 0.5 8 0.0 9 0.0 10 0.0 11 1.0 12 2.0 13 1.5 14 1.0 15 2.0 16 6.5 17 10.0 18 11.5 19 17.0 20 28.5 21 36.0 22 49.0 23 74.5 24 88.5 25 90.0 26 142.5 27 233.5 28 324.5 29 377.0 30 412.5 31 487.5 32 661.0 33 795.0 34 866.0 35 1029.5 36 1243.0 37 1364.0 38 1544.5 39 2109.0 40 3339.5 41 4904.0 42 5622.0 43 5686.0 44 5431.0 45 4575.0 46 4038.0 47 3741.5 48 3185.5 49 2675.0 50 2424.0 51 2261.5 52 1919.0 53 1606.5 54 1474.0 55 1410.0 56 1182.5 57 901.5 58 784.0 59 691.0 60 524.0 61 374.0 62 298.0 63 245.5 64 179.0 65 137.0 66 77.0 67 45.0 68 39.0 69 22.0 70 8.5 71 6.0 72 3.5 73 0.5 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.5 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 54457.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.44267954532934 #Duplication Level Percentage of deduplicated Percentage of total 1 68.85422469823584 34.04337367096976 2 16.846796657381617 16.659015369924894 3 6.677808727948004 9.905062710028096 4 2.9935004642525533 5.920267366913345 5 1.6490250696378832 4.076610904016013 6 0.9545032497678737 2.831591898194906 7 0.5756731662024142 1.9923976715573757 8 0.3714020427112349 1.4690489744201847 9 0.3045496750232126 1.3551976789026203 >10 0.6945218198700093 4.912132508217493 >50 0.033426183844011144 1.0797509961988356 >100 0.033426183844011144 3.3145417485355417 >500 0.0037140204271123496 1.8234570394990541 >1k 0.007428040854224699 10.617551462621885 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTCTT 3035 5.573204546706576 Illumina PCR Primer Index 2 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGT 2747 5.044346915915309 No Hit CTTTACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTC 993 1.8234570394990541 No Hit CTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTC 466 0.8557210275997577 No Hit TCTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCG 308 0.5655838551517711 No Hit TATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTCT 259 0.47560460546853484 No Hit TCTTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCC 146 0.2681014378316837 No Hit CTTAATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCG 143 0.26259250417760804 No Hit CTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATATCGTATGCCGT 130 0.23872045834328004 No Hit CTCTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCC 129 0.2368841471252548 No Hit CTTATAACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCG 114 0.20933947885487633 No Hit TCTTAATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCC 110 0.20199423398277538 No Hit ATACACATCTCCGAGCCCACGAGACCCGTTTGTAACTCGTATGCCGTCTT 85 0.15608645353214465 No Hit CTTAACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTC 72 0.13221440769781662 No Hit TTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTC 71 0.1303780964797914 No Hit ATACACATCTCCGAGCCCACGAGACCCGTTTGTACCTCGTATGCCGTCTT 70 0.12854178526176616 No Hit CTTATACACATCTCCGAGCCCACGAGACCCGTTTGTACCTCGTATGCCGT 66 0.12119654038966524 No Hit CTTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCG 62 0.11385129551756432 No Hit CTTATACACATCTCCGAGCCCACGAGACCCGTTTGTAACTCGTATGCCGT 57 0.10466973942743817 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0018363112180252309 0.0 9 0.0 0.0 0.0 0.005508933654075693 0.0 10 0.0 0.0 0.0 0.0073452448721009235 0.0 11 0.0 0.0 0.0 0.011017867308151385 0.0 12 0.0 0.0 0.0 0.011017867308151385 0.0 13 0.0 0.0 0.0 0.02019942339827754 0.0 14 0.0 0.0 0.0 0.02019942339827754 0.0 15 0.0 0.0 0.0 0.02203573461630277 0.0 16 0.0 0.0 0.0 0.02203573461630277 0.0 17 0.0 0.0 0.0 0.023872045834328 0.0 18 0.0 0.0 0.0 0.025708357052353234 0.0 19 0.0 0.0 0.0 0.029380979488403694 0.0 20 0.0 0.0 0.0 0.033053601924454154 0.0 21 0.0 0.0 0.0 0.034889913142479384 0.0 22 0.0 0.0 0.0 0.03672622436050462 0.0 23 0.0 0.0 0.0 0.04407146923260554 0.0 24 0.0 0.0 0.0 0.04407146923260554 0.0 25 0.0 0.0 0.0 0.04407146923260554 0.0 26 0.0 0.0 0.0 0.047744091668656 0.0 27 0.0 0.0 0.0 0.06243458141285785 0.0 28 0.0 0.0 0.0 0.08997924968323631 0.0 29 0.0 0.0 0.0 0.11936022917164 0.0 30 0.0 0.0 0.0 0.15975907596819508 0.0 31 0.0 0.0 0.0 0.21117579007290155 0.0 32 0.0 0.0 0.0 0.2368841471252548 0.0 33 0.0 0.0 0.0 0.2772829939218099 0.0 34 0.0 0.0 0.0 0.32319077437244065 0.0 35 0.0 0.0 0.0 0.4168426464917274 0.0 36 0.0 0.0 0.0 0.5692564775878216 0.0 37 0.0 0.0 0.0 0.8024680022770259 0.0 38 0.0 0.0 0.0 1.1146409093413152 0.0 39 0.0 0.0 0.0 1.480066841728336 0.0 40 0.0 0.0 0.0 1.8914005545659878 0.0 41 0.0 0.0 0.0 2.3100795122757405 0.0 42 0.0 0.0 0.0 2.681014378316837 0.0 43 0.0 0.0 0.0 3.086839157500413 0.0 44 0.0 0.0 0.0 3.4724645132857117 0.0 45 0.0 0.0 0.0 3.8268725783645814 0.0 46 0.0 0.0 0.0 4.322676607231394 0.0 47 0.0 0.0 0.0 4.787263345391777 0.0 48 0.0 0.0 0.0 5.279394751822539 0.0 49 0.0 0.0 0.0 5.72561837780267 0.0 50 0.0 0.0 0.0 6.20673191692528 0.0 51 0.0 0.0 0.0 6.682336522393816 0.0 52 0.0 0.0 0.0 7.1616137502984 0.0 53 0.0 0.0 0.0 7.607837376278532 0.0 54 0.0 0.0 0.0 8.083441981747066 0.0 55 0.0 0.0 0.0 8.533338230163247 0.0 56 0.0 0.0 0.0 8.970380300053254 0.0 57 0.0 0.0 0.0 9.339478854876324 0.0 58 0.0 0.0 0.0 9.752648878932002 0.0 59 0.0 0.0 0.0 10.186018326385955 0.0 60 0.0 0.0 0.0 10.617551462621885 0.0 61 0.0 0.0 0.0 11.076629267128192 0.0 62 0.0 0.0 0.0 11.500817158492021 0.0 63 0.0 0.0 0.0 11.923168738637825 0.0 64 0.0 0.0 0.0 12.288594671024846 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTACAC 95 0.0 70.00001 2 TCCATAT 15 0.0022135081 70.0 12 ACGTTTG 15 0.0022135081 70.0 29 TCTTAAT 20 7.8610894E-5 70.0 1 CACGTTT 15 0.0022135081 70.0 28 CTTATAA 35 2.9232433E-7 60.0 1 GTCGCCT 20 0.006916701 52.5 39 ACACGTT 20 0.006916701 52.5 27 CTGTATC 20 0.006916701 52.5 30 GGGCTTG 20 0.006916701 52.5 1 ATCGTAT 40 7.3523006E-7 52.5 39 AATTGGT 20 0.006916701 52.5 60 AGACACG 20 0.006916701 52.5 25 CGAGACA 20 0.006916701 52.5 23 CTTTACA 120 0.0 52.5 1 GTCCATA 20 0.006916701 52.5 11 AAAGCGA 20 0.006916701 52.5 20 TTACACA 130 0.0 51.15385 3 TTGTATA 30 5.829927E-4 46.666664 33 ATATCGT 30 5.829927E-4 46.666664 37 >>END_MODULE