FastQCFastQC Report
Wed 25 May 2016
SRR1780453_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780453_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences541769
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCAG300.005757825429.31707428
CGCTCTA450.001403247424.42638233
AAACTCG1158.800271E-921.03181527
TTTACAC3400.020.6772142
ACATAAG755.453815E-520.5390281
GCCGTTA654.945779E-420.29643626
CACGGTT654.973578E-420.2795778
CTTTACA3500.020.1198651
TTACAAC550.004430358820.03889720
GAATACC550.004487660219.99445525
ATCGTAC550.004487660219.99445525
TCGTACT550.004494865419.98891426
CGAAACT1553.45608E-1119.8654625
CTATACA2500.019.365371
GTTCTAA708.100281E-418.8623731
TACTGAC1051.7857928E-618.8606311
ACGCGGC1303.5013727E-818.6498615
CTTCCGA600.007336131318.36388818
TACGACT600.007336131318.36388819
CGACCGC600.007343971618.36048717
Produced by FastQC (version 0.11.5)