FastQCFastQC Report
Wed 25 May 2016
SRR1780453_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780453_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences541769
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGT446748.24594984209137No Hit
ATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTCTT361746.6770154807676345RNA PCR Primer, Index 20 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTC101381.8712772417764767No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG58941.0879175441931894No Hit
CTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTC55031.0157465635722973No Hit
TATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTCT32530.6004404091042492No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCC26450.48821545714132775No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCC20330.37525218312601866No Hit
CTTAATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG17700.3267075081815312No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCC13420.24770704857605363No Hit
CTTATAACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG13260.2447537603665031No Hit
TTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTC9730.17959683924329373No Hit
CTTAACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCGTC8510.15707801664547066No Hit
CTTATTACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG6730.12422268531422063No Hit
CTTTATACACATCTCCGAGCCCACGAGACGAAATGCCATCTCGTATGCCG6710.12385352428802682No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT40100.042.7381544
TTTACAC11200.039.5003742
GCCGTCT44600.038.3766843
CTTTACA12200.036.2894441
CTATACA6700.034.8477861
CTTATAA1850.034.5279161
TTACACA12950.034.3324433
CGGATCC353.2155367E-431.42857244
TTATACA65750.030.8644352
CTTATAC66350.030.6411381
GCGAACT602.9426883E-729.33062723
CGTTGAT300.005744842329.33062735
AAGCGTG300.005744842329.33062737
CGCCGTC300.005744842329.33062727
TAGGTCA554.9473056E-628.0051695
CGAGCCT407.0308807E-427.49746129
TATACAC75100.027.1683313
TGCCGTC63200.027.0822842
TTAATAC3900.025.96072
TCTTTAT2650.025.7667681
Produced by FastQC (version 0.11.5)