FastQCFastQC Report
Wed 25 May 2016
SRR1780452_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780452_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95622
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCCG150.00221977169.9911117
GACGTCG150.00221977169.9911123
CAATTGG150.00221977169.9911123
GGTTCTG150.00221977169.9911123
CGAAGTG150.00221977169.9911131
ACTGGGG252.3814206E-455.992893
TGTTTAT200.00693613852.4933339
CTTTTTA200.00693613852.4933344
ACCTAGT200.00693613852.4933346
CCGAGCT200.00693613852.4933368
GCTCGGA200.00693613852.4933311
CAATCCC200.00693613852.4933367
ATTGGGC305.8559125E-446.6607452
TACAAGG305.8559125E-446.660742
GAGACCT350.001250784539.9949244
ACAAGGA350.001250784539.994923
CAGATAC501.6130069E-435.3094981
GTGGGGC400.00240989434.9955529
GCTGAGG400.00240989434.99555224
GGGCCTT400.00240989434.99555212
Produced by FastQC (version 0.11.5)